Article
Food Science & Technology
Phillip Luu, Vijay Singh Chhetri, Marlene E. Janes, Joan M. King, Achyut Adhikari
Summary: The study showed that low concentrations of gaseous chlorine dioxide can effectively reduce pathogen levels on strawberries and blueberries, with significant disinfection effects on E. coli O157:H7, Listeria monocytogenes, and Salmonella. The reduction in pathogen levels varied with treatment time depending on the type of pathogen.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Martha Sanchez-Tamayo, Blanca Ruiz-Llacsahuanga, Rawane Raad, William Kerr, Faith Critzer
Summary: This study evaluated the efficacy of incorporating natural antimicrobials into carnauba wax and applying it to apples. The results showed that carnauba wax containing 2% essential oils was the most effective treatment for inactivating bacterial foodborne pathogens on apples.
Article
Food Science & Technology
Amin N. Olaimat, Anas A. Al-Nabulsi, Tareq M. Osaili, Murad Al-Holy, Mahmoud Abu Ghoush, Hana Alkhalidy, Ziad W. Jaradat, Mutamed Ayyash, Richard A. Holley
Summary: The study demonstrates that low dose gamma irradiation can effectively reduce the risk of Salmonella enterica, Listeria monocytogenes, or Escherichia coli O157:H7 in hummus, regardless of whether they are unstressed or subjected to cold, heat, or desiccation stresses.
JOURNAL OF FOOD SCIENCE
(2022)
Article
Food Science & Technology
Gokhan Kursad Incili, Mehmet Emin Aydemir, Muzeyyen Akgol, Busra Kaya, Hilal Kanmaz, Gulsum Oksuztepe, Ali Adnan Hayaloglu
Summary: The study evaluated the efficacy of Rheum Ribes L. juice as a marinade against bacteria in raw beef, showing significant reductions in E. coli O157:H7, S. Typhimurium, and L. monocytogenes counts at 100% concentration for 24 hours. The juice contained 22 volatile compounds, with ethanol, ethylbenzene, and xylene as major components, exhibiting strong antimicrobial activity. The results suggest that Rheum Ribes L. juice can be used as an acidulant for decontaminating meat and as a key ingredient in marinades.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Wei Fan, Xiao-yue Gao, He-nan Li, Wen-ping Guo, Ying-ying Li, Shou-wei Wang
Summary: This study aimed to develop a method combining IMS and qPCR techniques for rapid and accurate detection of three pathogenic bacteria in meat samples. The method showed high capture efficiency, good repeatability, and low detection limits.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Eun-Jung Lee, Soo-Hwan Kim, Sang-Hyun Park
Summary: The objective of this study was to evaluate the survival and cell injury of Salmonella Typhimurium, Escherichia coli O157:H7, and Listeria monocytogenes in apple puree after high hydrostatic pressure (HHP) treatment. The results showed that increasing pressure level and lowering pH of apple puree led to larger reductions in microbial levels. E. coli O157:H7 exhibited higher resistance compared to the other two pathogens.
Article
Multidisciplinary Sciences
Alka Rani, Vivek B. Ravindran, Aravind Surapaneni, Esmaeil Shahsavari, Nagalakshmi Haleyur, Nitin Mantri, Andrew S. Ball
Summary: The study presents a rapid, simple, specific, sensitive, and portable method for detecting E. coli O157:H7 in food and water. Evaluating the effect of gene selection using rfbE, fliC, and stx gene targets, the method showed differences in detection limits and specificity. This RPA-LFA method could be a potential rapid and effective alternative to conventional methods for monitoring E. coli O157:H7 in food and water.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Tareq M. Osaili, Anas A. Al-Nabulsi, Fayeza Hasan, Dinesh K. Dhanasekaran, Ayman Z. S. Hussain, Leila Cheikh Ismail, Farah Naja, Hadia Radwan, MoezAlIslam Ezzat Faris, Amin N. Olaimat, Mutamed Ayyash, Reyad S. Obaid, Richard Holley
Summary: This study aimed to investigate the inhibitory effect of a yogurt-based marinade individually combined with three essential oils (eugenol, vanillin, and beta-resorcylic acid) on camel meat cubes inoculated with Listeria monocytogenes, Salmonella spp., and Escherichia coli O157:H7. The results showed that the combination of essential oils and marinade effectively suppressed the growth of these pathogens, with a more pronounced effect at 10℃.
JOURNAL OF FOOD PROTECTION
(2023)
Article
Food Science & Technology
Berta Polanco-Estibalez, Rodrigo Garcia-Santa-Cruz, Rui P. Queiros, Vinicio Serment-Moreno, Mario Gonzalez-Angulo, Carole Tonello-Samson, Maria D. Rivero-Perez
Summary: Cold brew coffee (CBC) is popular for its unique sensory experience, but improper storage can pose a risk for bacterial pathogens. High-Pressure Processing (HPP) is a nonthermal technology that can improve the safety of CBC while maintaining its quality. This study shows that HPP can effectively control pathogens and spoilage microorganisms in CBC while preserving its quality attributes.
Article
Microbiology
Hongzhi Zhang, Fengxia Que, Biyao Xu, Linjun Sun, Yanqi Zhu, Wenjie Chen, Yulong Ye, Qingli Dong, Hong Liu, Xi Zhang
Summary: Listeria monocytogenes was found in a ready-to-eat meat processing plant in Shanghai, with the majority of isolates originating from processing facilities, notably the ST5 (1/2b) strain. The study revealed the pathogen's ability to form biofilms, aiding its persistence in the environment. While standard sanitation procedures were effective, they were insufficient in completely removing L. monocytogenes, especially its biofilms.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Environmental Sciences
Anna Lepecka, Dorota Zielinska, Piotr Szymanski, Izabela Buras, Danuta Kolozyn-Krajewska
Summary: This study assessed the microbiological quality of ready-to-eat salads and found variations in quality among the tested products. The salads had high counts of yeast, molds, or Enterobacteriaceae, with some contaminated by E. coli and S. aureus. Salmonella spp. and L. monocytogenes were also detected in some salads. The results indicate unsatisfactory microbiological quality and highlight the need for manufacturers to improve hygiene practices and implement hurdle technology for reducing microbial contamination.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Food Science & Technology
Cristina Rodriguez-Melcon, Carlos Alonso-Calleja, Rosa Capita
Summary: The study investigated the effects of low doses of four biocides on antibiotic resistance and cell surface hydrophobicity of Listeria monocytogenes and Salmonella enterica. The results showed that the use of subinhibitory concentrations of biocides could increase bacterial resistance to antibiotics and modify the hydrophobicity of the cell surface.
Article
Multidisciplinary Sciences
Alan Gutierrez, Keith R. Schneider
Summary: The use of poultry litter as a biological soil amendment increases the risk of preharvest contamination of fresh produce by Salmonella. This study showed that the presence of indigenous microflora in poultry litter does not significantly affect the survival of Salmonella.
Article
Food Science & Technology
Yangjin Jung, Mengqi Guo, Jingwen Gao, Hyein Jang, Karl R. Matthews
Summary: This study compared the efficacy of commercially available antimicrobials and tap water in preventing cross-contamination during the soaking step of crisping. The results showed that electrolyzed water was the most effective in completely eliminating cross-contamination of foodborne pathogens.
FOOD QUALITY AND SAFETY
(2022)
Article
Food Science & Technology
Yangjin Jung, Mengqi Guo, Jingwen Gao, Hyein Jang, Karl R. Matthews
Summary: The study compared the efficacy of commercially available antimicrobials, including electrolyzed water, lactic acid and phosphoric acid-based, and citric acid-based, with tap water in preventing cross-contamination during the crisping process in a retail setting. The results showed that electrolyzed water was the most effective in reducing contamination.
FOOD QUALITY AND SAFETY
(2022)
Article
Biotechnology & Applied Microbiology
Xingjian Bai, Dongqi Liu, Luping Xu, Shivendra Tenguria, Rishi Drolia, Nicholas L. F. Gallina, Abigail D. Cox, Ok-Kyung Koo, Arun K. Bhunia
Summary: Environmental signals play a role in promoting microbial biofilm formation and genetic heterogeneity. Biofilms produced by pathogens in food production facilities are a major source of contamination, but the mechanisms of biofilm-isolated sessile cells are not well understood. Studies show that the virulence of biofilm-isolated Lm is temporarily attenuated in mice during the early stage of infection (12-24 hours), but fully restored at a later stage (48 hours).
NPJ BIOFILMS AND MICROBIOMES
(2021)
Review
Microbiology
Leonardo Lopes-Luz, Marcelo Mendonca, Matheus Bernardes Fogaca, Andre Kipnis, Arun K. Bhunia, Samira Buhrer-Sekula
Summary: Listeria monocytogenes is a highly invasive foodborne pathogen responsible for numerous global outbreaks. Traditional bacterial culture methods for detection are time-consuming, while molecular methods are costly and require specific knowledge. Immunological approaches offer faster, simpler alternatives for detecting L. monocytogenes in various samples, with improved sensitivity and specificity but still facing challenges.
CRITICAL REVIEWS IN MICROBIOLOGY
(2021)
Article
Engineering, Environmental
Qiyuan Sun, Shanbin Wu, Ran Yin, Xingjian Bai, Arun K. Bhunia, Changqing Liu, Yuyi Zheng, Feifeng Wang, Ernest R. Blatchley
Summary: The molecular weight of fulvic acid has a significant impact on the degradation of MC-LR in the chlorine/UV process, with small molecular weight FA inhibiting further degradation of MC-LR and reducing its cytotoxicity.
Review
Biochemical Research Methods
Matheus Bernardes Torres Fogaca, Arun K. Bhunia, Leonardo Lopes-Luz, Eduardo Pimenta Ribeiro Pontes de Almeida, Jose Daniel Goncalves Vieira, Samira Buhrer-Sekula
Summary: The use of lateral flow immunoassay (LFIA) and nucleic acid-based lateral flow immunoassay (NALFIA) can improve the sensitivity and specificity of Listeria monocytogenes detection, accelerating the testing process. By improving the ligands and configuration of assay kits, the detection sensitivity and automated interpretation of results in LFIA can be enhanced. Integration of surface-enhanced Raman spectroscopy and immunomagnetic separation technologies can significantly improve the sensitivity of LFIA for rapid food safety monitoring.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Xingjian Bai, Luping Xu, Atul Kumar Singh, Xiaoling Qiu, Mai Liu, Ahmed Abuzeid, Talaat El-Khateib, Arun K. Bhunia
Summary: This study aims to control mixed-culture biofilms in the food processing environment using food-grade natural nanoparticulated antimicrobials. The results showed that ChNP-PL exhibited a synergistic antimicrobial and anti-biofilm effect against mixed-culture biofilms of foodborne pathogens, making it a promising method to prevent or eliminate these biofilms.
Article
Biotechnology & Applied Microbiology
Yezhi Fu, Arun K. Bhunia, Yuan Yao
Summary: The study evaluated the efficacy of an alginate-based antimicrobial seed coating in reducing foodborne pathogens from alfalfa seeds and sprouts. Results showed that the coating reduced both pathogens to an undetectable level one day after treatment, indicating its potential in reducing bacterial load. Further research is needed to optimize antimicrobial compounds and coating materials to minimize impact on seed germination rate.
Article
Chemistry, Applied
Harrison Helmick, Christabel Hartanto, Sarah Ettestad, Andrea Liceaga, Arun K. Bhunia, Jozef L. Kokini
Summary: This study investigated the modification of pea protein structure and its impact on gel formation. The results showed that applying shear forces at low temperatures in ethanol led to the formation of hydrophobically bound gels, which may be used to produce low-fat food products with desired textures.
FOOD HYDROCOLLOIDS
(2023)
Article
Nanoscience & Nanotechnology
Jose Waimin, Sarath Gopalakrishnan, Ulisses Heredia-Rivera, Nicholas A. Kerr, Sina Nejati, Nicholas L. F. Gallina, Arun K. Bhunia, Rahim Rahimi
Summary: Contamination of meat with pathogenic microorganisms can lead to severe illnesses and food waste, causing negative impacts on health and the economy. Wireless pH monitoring is crucial for detecting spoilage on a large commercial scale. To address this need, we have developed a cost-effective sensor that uses pH-responsive polymer to remotely assess the risk of microbial spoilage throughout the supply chain.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Chemistry, Analytical
Rashad R. Al-Hindi, Addisu D. Teklemariam, Mona G. Alharbi, Ibrahim Alotibi, Sheren A. Azhari, Ishtiaq Qadri, Turki Alamri, Steve Harakeh, Bruce M. Applegate, Arun K. Bhunia
Summary: Foodborne microorganisms, especially bacterial pathogens, are a major cause of human illness worldwide. Conventional detection methods for these pathogens have limitations, but phage-based biosensors offer increased accuracy, specificity, and shorter assay times, making them a promising tool for food safety.
Article
Food Science & Technology
Manoj Sawale, Xiang Cheng, Rishi Drolia, Patnarin Benyathiar, Ferhan Ozadali, Arun Bhunia, Dharmendra Mishra
Summary: This study aims to develop a methodology for estimating the kinetic inactivation parameters of B. atrophaeus using liquid hydrogen peroxide as a sterilant. The log-linear and Weibull models were found to be suitable for estimating the sterilization process at different temperatures and peroxide concentrations, with reasonably accurate parameter estimates.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Editorial Material
Immunology
Arun K. Bhunia, Rana Al-Sadi
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Immunology
Arun K. K. Bhunia, Atul K. K. Singh, Kyle Parker, Bruce M. M. Applegate
Summary: Classical microbiology played a crucial role in the development of modern biotechnology and microbial biosensing platforms. The invention of the Petri plate revolutionized the field of microbiology and continues to have a profound impact on isolating, identifying, and studying microorganisms. The use of sensing devices to detect and identify bacteria directly on the plate has the potential to further revolutionize microbiology, particularly in gene sequencing, pathogenicity study, and characterizing industrially beneficial traits.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Food Science & Technology
Chuyan Chen, Claudia P. Coronel-Aguilera, Bruce M. Applegate, Andrew G. Gehring, Arun K. Bhunia, George C. Paoli
Summary: The USDA-FSIS has zero tolerance for E. coli O157:H7 in raw ground beef and currently collects samples from beef processing facilities for testing. This study assessed the potential of using a phage-based luminescence detection assay during sample transportation, and the results suggest that this approach is feasible.
Article
Cell Biology
Dongqi Liu, Xingjian Bai, Harrison D. B. Helmick, Manalee Samaddar, Mary Anne Roshni Amalaradjou, Xilin Li, Shivendra Tenguria, Nicholas L. F. Gallina, Luping Xu, Rishi Drolia, Uma K. Aryal, Gustavo Marcal Schmidt Garcia Moreira, Michael Hust, Mohamed N. Seleem, Jozef L. Kokini, Raluca Ostafe, Abigail Cox, Arun K. Bhunia
Summary: In this study, it is discovered that internalin B (InlB) is the main ligand of listeria adhesion protein (LAP) and their interaction is critical for listeria monocytogenes (Lm) to cross the intestinal epithelium. Deletion or deficiency of InlB reduces LAP-InlB interaction and LAP-mediated pathology in mice. InlB overexpression in non-pathogenic listeria innocua also leads to LAP-InlB interaction. In silico analysis reveals that a pocket region in the C-terminal domain of tetrameric LAP is the binding site for InlB. Mutations in negatively charged amino acids in the C terminus of LAP confirm altered binding conformation and weaker affinity for InlB. InlB transforms the housekeeping enzyme, AdhE (LAP), into a moonlighting pathogenic factor by binding to the cell surface.
Review
Food Science & Technology
Addisu D. Teklemariam, Rashad R. Al-Hindi, Raed S. Albiheyri, Mona G. Alharbi, Mashail A. Alghamdi, Amani A. R. Filimban, Abdullah S. Al Mutiri, Abdullah M. Al-Alyani, Mazen S. Alseghayer, Abdulaziz M. Almaneea, Abdulgader H. Albar, Mohsen A. Khormi, Arun K. Bhunia
Summary: Salmonella is a common zoonotic foodborne pathogen that poses a global public health threat. Transmission of Salmonella to humans occurs through contaminated animal- and plant-derived foods along the farm-to-fork continuum. The virulence factors of Salmonella, including capsule, adhesion proteins, flagella, plasmids, and type III secretion systems, play crucial roles in attaching, invading, and evading the host defense system. The increasing global prevalence of non-typhoidal Salmonella (NTS) serovars and the emergence of antibiotic-resistant variants highlight the need for effective control measures to ensure food safety.
Article
Biotechnology & Applied Microbiology
Lihan Huang, Nurul Hawa Ahmad, Vijay Juneja, Erika Stapp-Kamotani, Jose Gabiola, Udit Minocha, Robert Phillips, Marie Hooker, Isabel Walls, Kim Cook, James Lindsay
Summary: This study investigated the growth kinetics of foodborne pathogen B. cereus during the commercial production of liquid egg yolk using PLA(2) treatment. The results showed that B. cytotoxicus NVH391-98 can grow prolifically at 50 degrees C, with estimated minimum, optimum, and maximum temperatures of 16.7-18.5, 47.8-48.1, and 52.1-52.4 degrees C, respectively. The dynamic model was validated with isothermal curves and it was found that holding the temperature below 15 degrees C or above 55 degrees C can effectively prevent the growth of B. cytotoxicus in egg yolk.
Article
Biotechnology & Applied Microbiology
Giacomo Mantegazza, Robin Duncan, Nicolo Telesca, Giorgio Gargari, Susanna Perotti, Patrizia Riso, Simone Guglielmetti
Summary: This study investigates whether salad-associated LAB can survive the gastrointestinal transit (GIT) and contribute to the gut microbiota. The results suggest that salad-associated LAB can survive in the gastrointestinal tract, but their quantity does not significantly alter the composition of the gut microbiota.
Article
Biotechnology & Applied Microbiology
Itsaso Baztarrika, Adrian Salazar-Sanchez, Lorena Laorden, Ilargi Martinez-Ballesteros, Rodrigo Alonso, Irati Martinez-Malaxetxebarria
Summary: The infection mechanisms of Arcobacter are still uncertain. This study found that Arcobacter isolates from food and water sources were able to adhere and invade Caco-2 cells, and carried at least two virulence markers. A. butzleri showed higher virulent activity compared to A. cryaerophilus, while A. lanthieri, A. skirrowii, and A. vitoriensis exhibited even higher adhesion and invasion values. Sewage isolates were more adherent and invasive, and had a higher gene content. The association between cadF and hecA with high adhesion capability was identified for the first time.
Article
Biotechnology & Applied Microbiology
Balaji Sundararaman, Karen Shapiro, Andrea Packham, Lauren E. Camp, Rachel S. Meyer, Beth Shapiro, Richard E. Green
Summary: Pathogenic bacteria, viruses, fungi, and protozoa can cause food and waterborne diseases, and surveillance methods are needed. In this study, CNER method was used to prepare enrichment baits for difficult-to-grow pathogens. This method allows direct sequencing of pathogens from samples.
Article
Biotechnology & Applied Microbiology
Guanghui Zhou, Pengcheng Dong, Xin Luo, Lixian Zhu, Yanwei Mao, Yunge Liu, Yimin Zhang
Summary: Interactions between bacterial species in the formation of dual-species biofilms in the food industry are affected by environmental stresses, such as temperature and pH. This study found that the combination of cold and acid stresses significantly increased biofilm formation, and different temperatures resulted in variations in the presence of specific bacterial species and the secretion of extracellular substances. The findings provide valuable insights for addressing mixed biofilms in food processing under diverse stressful conditions.
Article
Biotechnology & Applied Microbiology
Carmen Sanchez, Sonia Garde, Jose Maria Landete, Javier Calzada, Dave J. Baker, Rhiannon Evans, Arjan Narbad, Melinda J. Mayer, Marta Avila
Summary: This study sequenced and compared the genomes of bacteriophages active against C. tyrobutyricum, identifying novel species and phage proteins. A phage showed high lytic activity against C. tyrobutyricum cells and remained stable under different environmental conditions. Additionally, engineered strains successfully delivered endolysin and fluorescent protein to dairy products without affecting their technological properties.
Article
Biotechnology & Applied Microbiology
Danai Etter, Michael Biggel, Mariella Greutmann, Nicole Cernela, Sophia Johler
Summary: Bacillus cytotoxicus is a thermotolerant bacterium that is linked to rare but sometimes fatal cases of diarrheal disease. It is mostly found in dehydrated foods containing potato starch or insects and may be missed in routine screening.
Article
Biotechnology & Applied Microbiology
Tong Jin, Xiangjun Zhan, Liuxin Pang, Bo Peng, Xinpeng Zhang, Wenxiu Zhu, Baowei Yang, Xiaodong Xia
Summary: The study found that CpxAR TCS is an important regulatory mechanism for the virulence of C. sakazakii. Mutants showed weakened adhesion, invasion, and survival ability, and exhibited attenuated virulence in animal models. In addition, CpxAR TCS is involved in regulating the expression of genes associated with bacterial virulence and cellular invasion.
Article
Biotechnology & Applied Microbiology
Soo-Hwan Kim, Sang-Hyun Park, Jun-Bae Ahn, Dong-Hyun Kang
Summary: In this study, a semi continuous SHS system was developed to inactivate foodborne pathogens on radish seed surfaces and enhance the seeds' salinity tolerance. The results showed that higher temperature and more treatments led to a significant reduction in microbial count on the seed surface.
Article
Biotechnology & Applied Microbiology
Yujun Zhai, Weina Tian, Kewei Chen, Linshu Lan, Jianquan Kan, Hui Shi
Summary: This research investigates the role of flagella-related genes in the adhesion of E. coli O157:H7 and the effects of certain cations on bacterial adhesion and motility. The results demonstrate that flagellar-mediated motility is critical in the early stage of adhesion, and the presence of Fe2+, Ca2+, and Mn2+ affects the adhesion and motility of wildtype strains but not flagellar function-impaired strains. These findings provide new insights into the mechanisms of bacterial adhesion and have implications for the development of anti-adhesion agents.
Article
Biotechnology & Applied Microbiology
Mariana Marggraf, Andrea Barac, Jens Andre Hammerl, Stefan Hertwig
Summary: Isolating Yersinia enterocolitica from food is challenging due to overgrowth by other microflora and the lack of a selective medium. The authors improved the standard isolation procedure, resulting in a more sensitive method, reduced background bacteria, and time savings.
Article
Biotechnology & Applied Microbiology
Rafael D. Chaves, Samuel H. Kumazawa, Amin Mousavi Khaneghah, Veronica O. Alvarenga, Humberto M. Hungaro, Anderson S. Sant'Ana
Summary: This study investigated the susceptibility to sanitizers and biofilm-forming ability of 43 Salmonella enterica and Listeria monocytogenes strains on stainless steel surfaces. The results showed variations in susceptibility to sanitizers and biofilm resistance among different strains of the pathogens, with chlorine compounds being more effective in inactivating planktonic cells and biofilms.
Article
Biotechnology & Applied Microbiology
So Yeong Mun, Wooje Lee, Soo-Young Lee, Ji Yoon Chang, Hae Choon Chang
Summary: This study reveals that lactobacilli with a strong defense system dominate the final stage of kimchi fermentation and proposes a model for the succession mechanism of kimchi lactobacilli.
