期刊
FOOD MICROBIOLOGY
卷 29, 期 1, 页码 141-147出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fm.2011.09.014
关键词
Escherichia coli O157; Oxidative stress; Stress response; Shiga toxin; Transcriptomic analysis
资金
- Dairy Farmers of Ontario
- Natural Science and Engineering Research Council of Canada
Targeted microarray analysis was used to examine growth phase-related transcriptional responses of Escherichia coli O157:H7 stress and virulence genes. Logarithmic and stationary phase cells were exposed to hydrogen peroxide (H2O2; 1 and 2.5 mM) and paraquat (PQ; 350 mu M) for 40 min. For both oxidants, more genes were significantly induced in logarithmic cells compared to cells in stationary phase. In total, 14 genes at 1 mM H2O2 and 15 genes at 2.5 mM H2O2 were significantly induced in logarithmic cells, with 11 affected genes common between treatments, including stx2a and stx2b. At 2.5 mM H2O2, additional virulence genes were induced, including stx1a, stx1b, lpfA, a putative enterotoxin, and ler. Stx2 ELISA showed transcriptional induction of stx2a and stx2b correlated with significantly increased Stx2 in cultures exposed to 1 and 2.5 mM H2O2. Logarithmic cells significantly induced 15 genes following PQ exposure, 12 of which were induced in H2O2-treated cells, including several virulence genes (stx1a, stx2a, and stx2b). Exposure of stationary phase cells to H2O2 and PQ had little effect on the queried transcriptome, demonstrating the importance of considering this inherently stress resistant state when evaluating food safety interventions. (C) 2011 Elsevier Ltd. All rights reserved.
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