Article
Immunology
Ming Fu, Nini Chen, Yanhe Zhou, Sidong Chen, Wanfu Xu, Sitang Gong, Lanlan Geng
Summary: Human norovirus (HNV) is a global cause of epidemic viral gastroenteritis and lacks suitable animal models for research. This study investigated the effects of Jatrorrhizine (JAT), a natural alkaloid, on norovirus gastroenteritis. The results showed that JAT could inhibit NLRP3 inflammasome activation, decrease pyroptosis, and reduce replication of murine norovirus (MNV), suggesting its potential as a therapeutic agent.
Article
Agriculture, Dairy & Animal Science
Silvia Tofani, Giovanni Ianiro, Luca De Sabato, Marina Monini, Giorgia Angeloni, Eleonora Ponterio, Claudia D'Agostino, Michele Angelo Di Bari, Mauro Valeri, Ilaria Di Bartolo
Summary: Noroviruses are a major cause of acute viral gastroenteritis worldwide, with the ability to infect multiple species of mice. A study in an Italian research facility found that two variants of murine norovirus were circulating among healthy mice in 2011 and 2014, with full genome sequencing showing correlation with strains circulating in Europe. Understanding the molecular epidemiology of this virus can provide insights into its natural history and evolution in mice.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Di Liu, Yating Zheng, Yupeng Yang, Xinyan Xu, Hongtao Kang, Qian Jiang, Mingfa Yang, Liandong Qu, Jiasen Liu
Summary: A rapid and reliable diagnosis tool, the ERA-LFD assay, was developed for the detection of feline calicivirus (FCV). It showed high sensitivity and specificity, and was able to detect different strains and mutations of FCV.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Infectious Diseases
Francesco Pellegrini, Michele Camero, Cristiana Catella, Giuseppe Fracchiolla, Sabina Sblano, Giovanni Patruno, Claudia Maria Trombetta, Michela Galgano, Annamaria Pratelli, Maria Tempesta, Vito Martella, Gianvito Lanave
Summary: Norovirus (NoV) is a common cause of acute gastrointestinal illness worldwide, and there is currently no in vitro cell culture system for studying human NoV. This study used cultivatable caliciviruses as surrogates to evaluate antivirals. Lemon essential oil (LEO) was found to have virucidal efficacy against FCV in vitro.
Article
Veterinary Sciences
Jingfei Ye, Zhijie Li, Fei Yan Sun, Li Guo, Erkai Feng, Xue Bai, Yuening Cheng
Summary: In this study, a novel triple NanoPCR molecular detection method was established for FCV, FPV, and FHV-1, with higher sensitivity compared to conventional PCR. The clinical results showed positive rates of FCV, FPV, and FHV-1 were higher in NanoPCR tests than in conventional PCR, indicating the potential for wider clinical applications.
BMC VETERINARY RESEARCH
(2022)
Article
Virology
Shigeru Fujita, Ryota Koba, Yukinobu Tohya
Summary: The study identified several amino acid substitution sites in the P1 domain of VP1 that are involved in decreasing the binding of 1D7, which is a monoclonal antibody with unique ability to neutralize various FCV strains. These substitution sites are highly conserved among FCV strains compared to other neutralization-resistant variants found in the hypervariable region (HVR).
Article
Virology
Irene A. Owusu, Karla D. Passalacqua, Carmen Mirabelli, Jia Lu, Vivienne L. Young, Myra Hosmillo, Osbourne Quaye, Ian Goodfellow, Vernon K. Ward, Christiane E. Wobus
Summary: Akt activation plays a role in the late stage of murine norovirus infection, affecting viral replication, assembly, and cellular egress in a strain-dependent manner.
JOURNAL OF VIROLOGY
(2022)
Review
Biochemistry & Molecular Biology
Jolanta Sarowska, Dorota Wojnicz, Agnieszka Jama-Kmiecik, Magdalena Frej-Madrzak, Irena Choroszy-Krol
Summary: This review focuses on the antiviral activity of plant metabolites against human noroviruses. The chemical compositions of plant preparations greatly impact their biological activity. New strategies are essential in combating intestinal viruses to ensure food safety, reduce infections, and lower direct health costs.
Article
Multidisciplinary Sciences
Liya Hu, Wilhelm Salmen, Rong Chen, Yi Zhou, Frederick Neill, James E. Crowe, Robert L. Atmar, Mary K. Estes, B. V. Venkataram Prasad
Summary: This study reveals the structure and features of human norovirus GII.4 virus-like particles using X-ray crystallography and cryo-EM. It uncovers the adaptability and stability of the capsid protein VP1, as well as its potential antigen presentation mechanism, providing valuable insights for vaccine development.
NATURE COMMUNICATIONS
(2022)
Article
Virology
Hongxia Wu, Jiapei Huang, Yongxiang Liu, Yudi Pan, Yin Li, Qian Miao, Liandong Qu, Jin Tian
Summary: This study reveals that FCV infection induces degradation of host mRNAs and gene shutoff through PP protein, which is essential for mRNA degradation. The PP protein from different strains of FCV is a key factor in mRNA degradation, and the GST-PP fusion protein demonstrates RNase activity in vitro, potentially inhibiting the host immune response.
JOURNAL OF VIROLOGY
(2021)
Article
Food Science & Technology
Hyojin Kwon, Zhaoqi Wang, Hyelim Gu, Sumin Hwang, Youngmin Hwang, Jihoon An, Dong-Un Lee, Myeong-In Jeong, Changsun Choi
Summary: This study investigated effective washing conditions to reduce the microbial contamination of potatoes by using soaking and shaking in the washing process. The combined process of soaking-shaking I-shaking II was effective to eliminate E. coli, L. monocytogenes, and MNV-1 from potatoes during the handling and washing process. The reduction of E. coli in the washing process was higher than that of L. monocytogenes by approximately 0.5 logs, and MNV-1 showed a reduction in the soaking and shaking steps with no significant reduction observed after the combination process.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Nan Cao, Zhihui Tang, Xiyu Zhang, Wanyan Li, Bingxin Li, Yunbo Tian, Danning Xu
Summary: Felines are crucial companions in human families, and the detection and intervention of common viruses like FCV, FHV-1, and FPV are essential for preventing severe infectious diseases in baby cats. A triplex TaqMan qRT-PCR assay with high sensitivity, excellent repeatability, and good specificity was developed in this study, showing higher accuracy compared to commercial kits for clinical diagnosis.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Gianvito Lanave, Alessio Buonavoglia, Francesco Pellegrini, Barbara Di Martino, Federica Di Profio, Georgia Diakoudi, Cristiana Catella, Ahmed H. Omar, Violetta I. Vasinioti, Roberta Cardone, Giacinto Santo, Vito Martella, Michele Camero
Summary: Feline calicivirus (FCV) is a highly contagious and genetically variable viral pathogen in domestic cats, causing upper respiratory tract disease, oral ulcerations, salivation, and gingivitis-stomatitis. In this study, a small outbreak of FCV infection in two household cats was monitored, and the complete genome sequencing revealed multiple nucleotide mutations. The findings suggest that a respiratory-origin FCV strain transmitted between the animals and retained the limping disease pathotype.
Article
Immunology
Emily W. Helm, Amy M. Peiper, Matthew Phillips, Caroline G. Williams, Michael B. Sherman, Theresa Kelley, Hong Q. Smith, Sorin O. Jacobs, Dhairya Shah, Sarah M. Tatum, Neha Iyer, Marco Grodzki, Joyce C. Morales C. Aparicio, Elizabeth A. Kennedy, Mikayla S. Manzi, Megan T. Baldridge, Thomas J. Smith, Stephanie M. Karst
Summary: Noroviruses are a major cause of severe childhood diarrhea and foodborne disease worldwide. However, the pathogenic mechanisms underlying norovirus diarrhea are poorly understood due to the lack of animal models. By studying a mouse model, researchers have found that the virulence of noroviruses is regulated by the responsiveness of the viral capsid to environmental cues, which affects receptor binding and infectivity. This study provides a link between environmentally-influenced biophysical features and the severity of norovirus disease.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Virology
Bo Chen, Haoyang Zhang, Hanhong Wang, Shoujun Li, Pei Zhou
Summary: Feline calicivirus (FCV) and feline herpesvirus type I (FHV-1) are common viral pathogens responsible for cat respiratory diseases. A dual enzymatic recombinase amplification (ERA) method was developed for the differential detection of FCV and FHV-1 using the Exo probe. This method demonstrated high performance with a detection limit of 10(1) copies for both viruses and no cross-reactions with other viruses. Clinical testing of 50 cat nasopharyngeal swabs showed positive rates of 40% for FCV, 14% for FHV-1, and a coinfection rate of 10%. These results were consistent with quantitative real-time PCR. The dual ERA method is an efficient clinical diagnostic tool for detecting FCV and FHV-1.
Article
Food Science & Technology
Kye-Hwan Byun, Sang Ha Han, Min Woo Choi, Si Hong Park, Sang-Do Ha
Summary: The study found that sublethal concentrations of bactericidal antibiotics increased the mutation frequency in L. monocytogenes, facilitated the adaptation of the bacterium to food-associated stress, and led to an increase in its pathogenicity.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Food Science & Technology
Jung Kyu Chae, Sangha Han, Duk Hyun Kim, Si Hong Park, Sang-Do Ha
Summary: Bacteria from the Propionibacterium genus were mixed to study the influence of different temperatures and pH levels on propionic acid production. The results showed that both temperature and pH had a significant impact on the production of propionic acid.
FOOD SCIENCE AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Md Iqbal Hossain, Md Furkanur Rahaman Mizan, Sazzad Hossen Toushik, Pantu Kumar Roy, Iqbal Kabir Jahid, Si Hong Park, Sang-Do Ha
Summary: Listeria monocytogenes is a foodborne pathogen with potent biofilm-forming capability. This study found that combining nisin with thymol and eugenol can significantly inhibit the formation of L. monocytogenes biofilms, with better results than using any individual compound alone.
Article
Food Science & Technology
Kye-Hwan Byun, Sang Ha Han, Min Woo Choi, Si Hong Park, Sang-Do Ha
Summary: This study evaluated the efficacy of a bacteriophage cocktail as a biocontrol agent against L. monocytogenes in celery and enoki mushroom. The results showed that the phage cocktail can effectively inhibit the growth of L. monocytogenes in these foods, making it a potential candidate for biocontrol purposes.
Article
Biotechnology & Applied Microbiology
Shamsun Nahar, Ha Lim Jeong, Ah Jin Cho, Jun-Ha Park, Sangha Han, Younsoo Kim, Si-Hong Park, Sang-Do Ha
Summary: This study investigated the biofilm reduction effects of a food-grade enzyme (ficin) and a common sanitizer (PAA) on Salmonella. Results showed that ficin could enhance the efficacy of PAA in reducing biofilm. The combined use of enzyme and sanitizer proved promising in addressing food safety issues.
Article
Biotechnology & Applied Microbiology
Kye-Hwan Byun, Kyung Won Na, Md Ashrafudoulla, Min Woo Choi, Sang Ha Han, Iksoon Kang, Si Hong Park, Sang-Do Ha
Summary: The combination treatment of PAA or LA with UV-C significantly reduced Salmonella Enteritidis biofilms on food contact surfaces and chicken skin.
Article
Food Science & Technology
Sazzad Hossen Toushik, Jun-Ha Park, Kyeongjun Kim, Md Ashrafudoulla, Mevo Senakpon Isaie Ulrich, Md Furkanur Rahman Mizan, Pantu Kumar Roy, Won-Bo Shim, Young-Mog Kim, Si Hong Park, Sang-Do Ha
Summary: The study shows that bioactive substances from lactic acid bacteria and plant-derived essential oils can act as biocidal agents, efficiently inhibiting biofilm formation on seafood processing surfaces and potentially serving as preservatives in the seafood industry.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Food Science & Technology
Ae Min, Md Iqbal Hossain, Soontag Jung, Daseul Yeo, Zhaoqi Wang, Mengxiao Song, Ziwei Zhao, Sunho Park, Changsun Choi
Summary: This study evaluated the antiviral efficacy of three disinfectants on food-contact surfaces using a carrier testing method. Ethanol and peracetic acid showed effective antiviral activity, while quaternary ammonium compounds were ineffective. Comparison with suspension testing showed that ethanol and peracetic acid exhibited the best antiviral efficacy.
Review
Food Science & Technology
Sangha Han, Seok-Woo Hyun, Jeong Won Son, Min Su Song, Dong Jae Lim, Changsun Choi, Si Hong Park, Sang-Do Ha
Summary: Various foodborne viruses have caused gastroenteritis and had a significant economic impact globally in the past decade. The emergence of new variants of infectious viruses continues to grow. Inactivating foodborne viruses in the food industry is challenging because they can survive in the food matrix during processing and storage. Conventional methods have limitations, necessitating more effective and environmentally friendly techniques. Nonthermal techniques are considered a new platform for safe and effective treatment to inactivate foodborne viruses.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2023)
Article
Biotechnology & Applied Microbiology
Soontag Jung, Daseul Yeo, Zhaoqi Wang, Seoyoung Woo, Yeeun Seo, Md Iqbal Hossain, Changsun Choi
Summary: Since the first SARS-CoV-2 outbreak in Wuhan, there has been concern over the link between SARS-CoV-2 transmission and food. This study evaluated the viability of SARS-CoV-2 on different types of food and found that the virus can persist longer at lower temperatures. Disinfectants such as 70% ethanol and ClO2 were effective in reducing the virus on food, while peracetic acid was able to eliminate it completely.
Article
Food Science & Technology
Yoonjeong Yoo, Miseon Sung, Jeongeun Hwang, Daseul Yeo, Ziwei Zhao, Changsun Choi, Yohan Yoon
Summary: This study estimated the risk of hepatitis A virus (HAV) foodborne illness outbreaks through the consumption of fermented clams in South Korea. The results showed that while there is a low likelihood of HAV foodborne illness from consuming fermented clams across the country, regular consumers should be aware of the possibility of foodborne illness.
Article
Food Science & Technology
Eun-Ji Han, Eun-Young Choi, Su -Ji Jeon, Sang-Woo Lee, Jun-Mo Moon, Soo-Hyun Jung, Bumseok Kim, Sung-Dae Cho, Jeong-Seok Nam, Changsun Choi, Jeong-Hwan Che, Ji- Youn Jung
Summary: This study found that Piperlongumine (PL) induces apoptosis in cervical cancer cells and demonstrated the relationship between apoptosis and autophagy. The anti-cancer effect of PL is mediated through inhibiting the PI3K/protein kinase B/mammalian target of rapamycin pathway, while inhibiting the PI3K pathway increases autophagy.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Food Science & Technology
Zhaoqi Wang, Md Iqbal Hossain, Daseul Yeo, Seoyoung Woo, Yeeun Seo, Soontag Jung, Hyojin Kwon, Myeong-In Jeong, Changsun Choi
Summary: This study aimed to efficiently detect foodborne viruses by inoculating murine norovirus 1 (MNV-1) and hepatitis A virus (HAV) into post-washing water (PWW) containing various PCR inhibitors. The paper filtration-coupled ultrafiltration (PFC-UF) method combined with the OneStep PCR Inhibitor Removal Kit successfully recovered viruses from samples with high soil content and PCR inhibitors, providing a promising platform for foodborne virus surveillance in washing water.
Article
Food Science & Technology
Sun Min Park, Changsun Choi, Min Suk Rhee
Summary: This study developed a method to detect Coxiella burnetii in meat and successfully detected the pathogen in high and low contaminated meat samples. The method has the potential to reveal the risk of meat-borne Q fever in humans.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Food Science & Technology
Sunho Park, Md Iqbal Hossain, Soontag Jung, Zhaoqi Wang, Daseul Yeo, Seoyoung Woo, Yeeun Seo, Myeong-In Jeong, Changsun Choi
Summary: Fresh root vegetables can be a potential reservoir for human pathogenic viruses, including adenovirus and norovirus. Prevalence of these viruses, as well as rotavirus, astrovirus, and hepatitis A virus, in pre- and post-washed root vegetables was investigated. Results showed that adenovirus and norovirus GII were only detected in pre-washed samples. Hepatitis A virus was detected in both pre- and post-washed sweet potatoes, while norovirus GI and astrovirus were not detected in any of the root vegetables. Further research is needed to confirm the hypothesis that root vegetables can serve as a potential source of viral foodborne diseases. Washing produce can help reduce the risk of contracting diseases from certain root vegetables.
Article
Biotechnology & Applied Microbiology
Lihan Huang, Nurul Hawa Ahmad, Vijay Juneja, Erika Stapp-Kamotani, Jose Gabiola, Udit Minocha, Robert Phillips, Marie Hooker, Isabel Walls, Kim Cook, James Lindsay
Summary: This study investigated the growth kinetics of foodborne pathogen B. cereus during the commercial production of liquid egg yolk using PLA(2) treatment. The results showed that B. cytotoxicus NVH391-98 can grow prolifically at 50 degrees C, with estimated minimum, optimum, and maximum temperatures of 16.7-18.5, 47.8-48.1, and 52.1-52.4 degrees C, respectively. The dynamic model was validated with isothermal curves and it was found that holding the temperature below 15 degrees C or above 55 degrees C can effectively prevent the growth of B. cytotoxicus in egg yolk.
Article
Biotechnology & Applied Microbiology
Giacomo Mantegazza, Robin Duncan, Nicolo Telesca, Giorgio Gargari, Susanna Perotti, Patrizia Riso, Simone Guglielmetti
Summary: This study investigates whether salad-associated LAB can survive the gastrointestinal transit (GIT) and contribute to the gut microbiota. The results suggest that salad-associated LAB can survive in the gastrointestinal tract, but their quantity does not significantly alter the composition of the gut microbiota.
Article
Biotechnology & Applied Microbiology
Itsaso Baztarrika, Adrian Salazar-Sanchez, Lorena Laorden, Ilargi Martinez-Ballesteros, Rodrigo Alonso, Irati Martinez-Malaxetxebarria
Summary: The infection mechanisms of Arcobacter are still uncertain. This study found that Arcobacter isolates from food and water sources were able to adhere and invade Caco-2 cells, and carried at least two virulence markers. A. butzleri showed higher virulent activity compared to A. cryaerophilus, while A. lanthieri, A. skirrowii, and A. vitoriensis exhibited even higher adhesion and invasion values. Sewage isolates were more adherent and invasive, and had a higher gene content. The association between cadF and hecA with high adhesion capability was identified for the first time.
Article
Biotechnology & Applied Microbiology
Balaji Sundararaman, Karen Shapiro, Andrea Packham, Lauren E. Camp, Rachel S. Meyer, Beth Shapiro, Richard E. Green
Summary: Pathogenic bacteria, viruses, fungi, and protozoa can cause food and waterborne diseases, and surveillance methods are needed. In this study, CNER method was used to prepare enrichment baits for difficult-to-grow pathogens. This method allows direct sequencing of pathogens from samples.
Article
Biotechnology & Applied Microbiology
Guanghui Zhou, Pengcheng Dong, Xin Luo, Lixian Zhu, Yanwei Mao, Yunge Liu, Yimin Zhang
Summary: Interactions between bacterial species in the formation of dual-species biofilms in the food industry are affected by environmental stresses, such as temperature and pH. This study found that the combination of cold and acid stresses significantly increased biofilm formation, and different temperatures resulted in variations in the presence of specific bacterial species and the secretion of extracellular substances. The findings provide valuable insights for addressing mixed biofilms in food processing under diverse stressful conditions.
Article
Biotechnology & Applied Microbiology
Carmen Sanchez, Sonia Garde, Jose Maria Landete, Javier Calzada, Dave J. Baker, Rhiannon Evans, Arjan Narbad, Melinda J. Mayer, Marta Avila
Summary: This study sequenced and compared the genomes of bacteriophages active against C. tyrobutyricum, identifying novel species and phage proteins. A phage showed high lytic activity against C. tyrobutyricum cells and remained stable under different environmental conditions. Additionally, engineered strains successfully delivered endolysin and fluorescent protein to dairy products without affecting their technological properties.
Article
Biotechnology & Applied Microbiology
Danai Etter, Michael Biggel, Mariella Greutmann, Nicole Cernela, Sophia Johler
Summary: Bacillus cytotoxicus is a thermotolerant bacterium that is linked to rare but sometimes fatal cases of diarrheal disease. It is mostly found in dehydrated foods containing potato starch or insects and may be missed in routine screening.
Article
Biotechnology & Applied Microbiology
Tong Jin, Xiangjun Zhan, Liuxin Pang, Bo Peng, Xinpeng Zhang, Wenxiu Zhu, Baowei Yang, Xiaodong Xia
Summary: The study found that CpxAR TCS is an important regulatory mechanism for the virulence of C. sakazakii. Mutants showed weakened adhesion, invasion, and survival ability, and exhibited attenuated virulence in animal models. In addition, CpxAR TCS is involved in regulating the expression of genes associated with bacterial virulence and cellular invasion.
Article
Biotechnology & Applied Microbiology
Soo-Hwan Kim, Sang-Hyun Park, Jun-Bae Ahn, Dong-Hyun Kang
Summary: In this study, a semi continuous SHS system was developed to inactivate foodborne pathogens on radish seed surfaces and enhance the seeds' salinity tolerance. The results showed that higher temperature and more treatments led to a significant reduction in microbial count on the seed surface.
Article
Biotechnology & Applied Microbiology
Yujun Zhai, Weina Tian, Kewei Chen, Linshu Lan, Jianquan Kan, Hui Shi
Summary: This research investigates the role of flagella-related genes in the adhesion of E. coli O157:H7 and the effects of certain cations on bacterial adhesion and motility. The results demonstrate that flagellar-mediated motility is critical in the early stage of adhesion, and the presence of Fe2+, Ca2+, and Mn2+ affects the adhesion and motility of wildtype strains but not flagellar function-impaired strains. These findings provide new insights into the mechanisms of bacterial adhesion and have implications for the development of anti-adhesion agents.
Article
Biotechnology & Applied Microbiology
Mariana Marggraf, Andrea Barac, Jens Andre Hammerl, Stefan Hertwig
Summary: Isolating Yersinia enterocolitica from food is challenging due to overgrowth by other microflora and the lack of a selective medium. The authors improved the standard isolation procedure, resulting in a more sensitive method, reduced background bacteria, and time savings.
Article
Biotechnology & Applied Microbiology
Rafael D. Chaves, Samuel H. Kumazawa, Amin Mousavi Khaneghah, Veronica O. Alvarenga, Humberto M. Hungaro, Anderson S. Sant'Ana
Summary: This study investigated the susceptibility to sanitizers and biofilm-forming ability of 43 Salmonella enterica and Listeria monocytogenes strains on stainless steel surfaces. The results showed variations in susceptibility to sanitizers and biofilm resistance among different strains of the pathogens, with chlorine compounds being more effective in inactivating planktonic cells and biofilms.
Article
Biotechnology & Applied Microbiology
So Yeong Mun, Wooje Lee, Soo-Young Lee, Ji Yoon Chang, Hae Choon Chang
Summary: This study reveals that lactobacilli with a strong defense system dominate the final stage of kimchi fermentation and proposes a model for the succession mechanism of kimchi lactobacilli.
