Review
Endocrinology & Metabolism
Federica Marelli, Giuditta Rurale, Luca Persani
Summary: This review provides an update on recent advancements in understanding the early steps of thyroid differentiation in zebrafish, highlighting the physiological processes and molecular mechanisms involved. The study reveals a complex crosstalk between intrinsic and extrinsic factors that are crucial for proper thyroid development, with various signaling pathways playing important roles in the commitment of endodermal cells to a thyroid fate during specific developmental windows in the zebrafish embryo.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Multidisciplinary Sciences
Rui Guo, Kangkang Ge, Yuying Wang, Minxia Lu, Fei Li, Lili Tian, Lin Gan, Donglai Sheng
Summary: LHX4 is expressed in the photoreceptors and bipolar cells of mouse and chicken retina, but its function in zebrafish retina was unknown. This study found that lhx4 is expressed in zebrafish retina and regulates neural differentiation and visual function during embryonic development.
SCIENTIFIC REPORTS
(2021)
Article
Developmental Biology
Benoit Haerlingen, Robert Opitz, Isabelle Vandernoot, Angelo Molinaro, Meghna Parakkal Shankar, Pierre Gillotay, Achim Trubiroha, Sabine Costagliola
Summary: Thyroid tissue is derived from ventral pharyngeal endoderm and defects in morphogenesis are a main cause of congenital thyroid diseases. Recent studies have revealed the importance of FGF and BMP signaling in thyroid specification, but the relationship between signaling cues and thyroid transcription factors is still unclear. By analyzing zebrafish embryos, researchers identified a population of Pax2a-expressing thyroid progenitor cells that show enhanced FGF signaling but lack Nkx2.4b expression and BMP signaling. Manipulation of FGF/BMP activities suggests that FGF signaling mainly regulates Pax2a expression, while BMP signaling regulates both Pax2a and Nkx2.4b expression. Similar expression dynamics were observed in mouse embryos, indicating that this refined model of thyroid cell specification is evolutionarily conserved in mammals.
Review
Biotechnology & Applied Microbiology
Daniela M. Figiel, Randa Elsayed, Andrew C. Nelson
Summary: Research has shown that zebrafish and humans share a high degree of genetic homology in the endodermal organ system, making zebrafish an attractive model organism for studying endoderm development and disease. Advances in biological and technological methods offer the potential for enhanced analysis of endoderm formation, while integration with human data allows for modeling the relationship between non-coding sequence variants and human disease.
BRIEFINGS IN FUNCTIONAL GENOMICS
(2021)
Article
Cell Biology
Carlos Camacho-Macorra, Marcos Sintes, Noemi Tabanera, Irene Grasa, Paola Bovolenta, Marcos J. Cardozo
Summary: Hedgehog (Hh) signaling pathway is highly regulated and mutations in its components can lead to a variety of congenital malformations. Mosmo, a modulator of the Hh pathway, plays a role in down-modulating pathway activation. Studies in zebrafish embryos suggest that MOSMO may be a candidate gene for uncharacterized forms of human congenital craniofacial malformations.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Immunology
Juan Hu, Xing Peng, Weina Du, Yichuan Huang, Chun Zhang, Xian Zhang
Summary: This study demonstrates that circSLC6A6 plays a role in promoting EC progression by regulating the PI4KB/hedgehog pathway through sponging miR-497-5p. The findings suggest that circSLC6A6 may serve as a potential novel biomarker for EC diagnosis or treatment.
JOURNAL OF IMMUNOLOGY RESEARCH
(2021)
Review
Cell Biology
Fatemeh Mirzadeh Azad, Isabelle Laurence Polignano, Valentina Proserpio, Salvatore Oliviero
Summary: Long noncoding RNAs play a crucial role in stem cell maintenance and differentiation, exhibiting diverse functions through interactions with proteins, DNA, and RNA. This heterogeneity in function enhances the capabilities of the lncRNome toolkit but also poses challenges in predicting the functions of novel lncRNAs.
TRENDS IN CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Qingxia Jin, Yuqing Hu, Yuqi Gao, Jiayi Zheng, Jun Chen, Ce Gao, Jinrong Peng
Summary: Genetic studies in zebrafish demonstrate that simultaneous loss-of-function of Hhex and Prox1a genes blocks hepatic differentiation from prospective hepatoblasts, resulting in a liverless phenotype.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Developmental Biology
Philipp M. Tschaikner, Dominik Regele, Ruth Roeck, Willi Salvenmoser, Dirk Meyer, Michel Bouvier, Stephan Geley, Eduard Stefan, Pia Aanstad
Summary: In this study, Gpr161 was found to play a crucial role in negatively regulating the Hh signaling pathway by activating PKA to inhibit the expression of Hh target genes. Additionally, PKA phosphorylation-deficient forms of Gpr161 were shown to increase sensitivity to Shh, resulting in excessive high-level Hh signaling. These findings suggest that PKA-mediated phosphorylation of Gpr161 may provide a mechanism for fine-tuning Gpr161 ciliary localization and PKA activity.
Review
Biochemistry & Molecular Biology
Matthew R. Hawkins, Rebecca A. Wingert
Summary: Retinoic acid, a metabolite of vitamin A, plays a significant role in development, influencing differentiation, patterning, and organogenesis. The conservation of RA and its pathways from zebrafish to humans makes zebrafish a valuable translational model for investigating the functions of RA and associated diseases. This review explores both foundational and recent studies using zebrafish to understand RA at the molecular and organismal level.
Article
Biotechnology & Applied Microbiology
Ping Wu, Pengzheng Yong, Zhanxiong Zhang, Rui Xu, Renjie Shang, Jun Shi, Jianshe Zhang, Pengpeng Bi, Elizabeth Chen, Shaojun Du
Summary: Research indicates that in zebrafish, slow-twitch muscle fibers remain mononucleated while fast-twitch fibers can fuse to form multinucleated fibers. Myomixer plays a crucial role in fusion of specific muscle types, and its genetic deletion leads to severe myopathy and uncontrolled adipose infiltration in both muscle tissues.
MARINE BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Tanveer Teranikar, Cameron Villarreal, Nabid Salehin, Toluwani Ijaseun, Jessica Lim, Cynthia Dominguez, Vivian Nguyen, Hung Cao, Cheng-Jen Chuong, Juhyun Lee
Summary: Quantitative assessment of structural and functional biomarkers in vivo is crucial for understanding congenital disorders. This study demonstrates the potential of LSFM as a non-invasive tool for evaluating cardiac mechano-transduction in zebrafish.
Article
Multidisciplinary Sciences
Jian Heng, Boyang Shi, Jia-Yi Zhou, Yifan Zhang, Dongyuan Ma, Yun-Gui Yang, Feng Liu
Summary: Cpeb1b-mediated cytoplasmic polyadenylation plays a crucial role in hematopoietic stem and progenitor cell development by regulating Hedgehog signaling. Cpeb1b controls hemogenic endothelium specification through the Hedgehog-Vegf-Notch axis, leading to the production of HSPCs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Neurosciences
Haritha Kunhiraman, Leon McSwain, Shubin W. Shahab, Timothy R. Gershon, Tobey J. MacDonald, Anna Marie Kenney
Summary: This study found that the Sonic Hedgehog (SHH) subtype of medulloblastoma (MB) has a unique tumor microenvironment compared to other subtypes. The SHH MB cells were found to produce elevated levels of IGFBP2, which is required for SHH MB cell proliferation, colony formation, and cell migration. These findings suggest that targeting the IGFBP2-STAT3 axis could be a novel therapeutic approach for SHH medulloblastoma.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Ana R. Lopez-Perez, Piotr J. Balwierz, Boris Lenhard, Ferenc Muller, Fiona C. Wardle, Isabelle Manfroid, Marianne L. Voz, Bernard Peers
Summary: In this study, the gene regulatory network triggered by retinoic acid (RA) in the endoderm of zebrafish was investigated using RNA-seq, RAR ChIP-seq, and ATAC-seq assays. The findings identified a set of genes and regulatory regions regulated by RA signaling, defined direct RAR target genes in zebrafish, revealed conserved direct target genes between zebrafish and mice, and unveiled the role of the RAR-direct targets in chromatin accessibility upon RA treatment.
SCIENTIFIC REPORTS
(2021)
Editorial Material
Cell Biology
Eduard Stefan, Jonathan R. Hart, Klaus Bister
Review
Endocrinology & Metabolism
O. Torres-Quesada, R. Roeck, E. Stefan
HORMONE AND METABOLIC RESEARCH
(2017)
Article
Biochemistry & Molecular Biology
Jessica G. H. Bruystens, Jian Wu, Audrey Fortezzo, Jason Del Rio, Cole Nielsen, Donald K. Blumenthal, Ruth Rock, Eduard Stefan, Susan S. Taylor
JOURNAL OF MOLECULAR BIOLOGY
(2016)
Article
Multidisciplinary Sciences
Verena A. Bachmann, Johanna E. Mayrhofer, Ronit Ilouz, Philipp Tschaikner, Philipp Raffeiner, Ruth Roeck, Mathieu Courcelles, Federico Apelt, Tsan-Wen Lu, George S. Baillie, Pierre Thibault, Pia Aanstad, Ulrich Stelzl, Susan S. Taylor, Eduard Stefan
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Cell Biology
L. Rinaldi, R. Delle Donne, M. Sepe, M. Porpora, C. Garbi, F. Chiuso, A. Gallo, S. Parisi, L. Russo, V. Bachmann, R. G. Huber, E. Stefan, T. Russo, A. Feliciello
CELL DEATH & DISEASE
(2016)
Article
Oncology
Philipp Raffeiner, Andrea Schraffl, Thomas Schwarz, Ruth Roeck, Karin Ledolter, Markus Hartl, Robert Konrat, Eduard Stefan, Klaus Bister
Article
Biochemistry & Molecular Biology
Kristofer J. Haushalter, Darren E. Casteel, Andrea Raffeiner, Eduard Stefan, Hemal H. Patel, Susan S. Taylor
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Multidisciplinary Sciences
Monia Porpora, Simona Sauchella, Laura Rinaldi, Rossella Delle Donne, Maria Sepe, Omar Torres-Quesada, Daniela Intartaglia, Corrado Garbi, Luigi Insabato, Margherita Santoriello, Verena A. Bachmann, Matthis Synofzik, Herbert H. Lindner, Ivan Conte, Eduard Stefan, Antonio Feliciello
NATURE COMMUNICATIONS
(2018)
Article
Multidisciplinary Sciences
Laura Rinaldi, Rossella Delle Donne, Bruno Catalanotti, Omar Torres-Quesada, Florian Enzler, Federica Moraca, Robert Nistico, Francesco Chiuso, Sonia Piccinin, Verena Bachmann, Herbert H. Lindner, Corrado Garbi, Antonella Scorziello, Nicola Antonino Russo, Matthis Synofzik, Ulrich Stelzl, Lucio Annunziato, Eduard Stefan, Antonio Feliciello
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Ruth Roeck, Johanna E. Mayrhofer, Omar Torres-Quesada, Florian Enzler, Andrea Raffeiner, Philipp Raffeiner, Andreas Feichtner, Roland G. Huber, Shohei Koide, Susan S. Taylor, Jakob Troppmair, Eduard Stefan
Article
Physiology
Nadine J. Ortner, Alexandra Pinggera, Nadja T. Hofer, Anita Siller, Niels Brandt, Andrea Raffeiner, Kristina Vilusic, Isabelle Lang, Kerstin Blum, Gerald J. Obermair, Eduard Stefan, Jutta Engel, Joerg Striessnig
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2020)
Review
Cell Biology
Philipp Tschaikner, Florian Enzler, Omar Torres-Quesada, Pia Aanstad, Eduard Stefan
Review
Oncology
Andreas Feichtner, Valentina Kugler, Selina Schwaighofer, Thomas Nuener, Jakob Fleischmann, Eduard Stefan
Summary: This article introduces a cell-based reporter system for identifying promising lead molecules. By analyzing the kinase activity states in cells, the specificity and efficacy of kinase inhibitors can be predicted.
MEMO-MAGAZINE OF EUROPEAN MEDICAL ONCOLOGY
(2022)
Article
Oncology
Omar Torres-Quesada, Carolina Doerrier, Sophie Strich, Erich Gnaiger, Eduard Stefan
Summary: This study examines the impact of a newly formulated human plasma-like media (HPLM) on cell growth, mitochondrial bioenergetics, and drug efficacy. It reveals that cells grown in HPLM display altered mitochondrial profiles and that an FDA-approved cancer drug has context-dependent side effects in HPLM. These findings emphasize the importance of selecting a more physiological cell culture medium for analyzing bioenergetic profiles and assessing drug efficacies.
Review
Cell Biology
Omar Torres-Quesada, Johanna E. Mayrhofer, Eduard Stefan
CELLULAR SIGNALLING
(2017)