Article
Biotechnology & Applied Microbiology
J. N. O'Sullivan, P. M. O'Connor, M. C. Rea, D. Field, C. Hill, R. P. Ross
Summary: This study aimed to investigate the expression of natural nisin variants produced by non-GRAS strains in a GRAS host. It successfully demonstrated the heterologous production of three natural nisin variants by a genetically modified GRAS strain, showcasing the potential for expanding lantibiotic production and development for future biotherapeutics.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Dusit Promrug, Kanjana Wittayacom, Nantipan Nathapanan, Ha Thanh Dong, Panumart Thongyoo, Sasimanus Unajuk, Onrapak Reamtong, Usa Boonyue, Amornrat Aroonnual, Tatsuo Shioda, Krit Thirapanmethee, Dumrongkiet Arthan
Summary: LAB isolated from red sea bass showed antimicrobial activity against Aeromonas species, and the cocultured Enterococcus faecium induced the highest antimicrobial activity. This finding is important for improving bacteriocin production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Mduduzi P. Mokoena, Cornelius A. Omatola, Ademola O. Olaniran
Summary: Lactic acid bacteria (LAB) are Gram-positive microorganisms used in fermented food production, producing bacteriocins and acids to control spoilage and pathogens. LAB and their bacteriocins are seen as alternatives to chemical food preservatives, with potential applications in food safety and novel food packaging materials.
Article
Immunology
Diogo Contente, Patricia Diaz-Rosales, Javier Feito, Lara Diaz-Formoso, Felix Docando, Rocio Simon, Juan Borrero, Pablo E. Hernandez, Patricia Poeta, Estefania Munoz-Atienza, Luis M. Cintas, Carolina Tafalla
Summary: Lactic Acid Bacteria (LAB) are frequently used as probiotics in aquaculture and have been shown to positively affect the growth, survival rate, and immunological status of fish. LAB produce antimicrobial peptides called bacteriocins, which have been well-studied for their antimicrobial properties. However, the direct immunomodulatory effects of bacteriocins in fish have not been extensively explored. In this study, the immunomodulatory effects of different bacteriocin-producing LAB strains were compared, and it was found that these strains had varying effects on immune functions, with nisin Z being the most potent immunomodulator.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Microbiology
Gee-Hyeun Choi, Wilhelm Heinrich Holzapfel, Svetoslav Dimitrov Todorov
Summary: It has been almost a century since the discovery of penicillin, and during this time, there has been great enthusiasm and abuse in the development and use of antibiotics. However, the emergence of antibiotic resistance has led to the realization that new types of antimicrobials are needed. Bacteriocins, produced by various types of bacteria and other organisms, have been explored as potential antimicrobials, particularly in the food industry. In recent decades, bacteriocins have shown promise as alternative therapies for treating antibiotic-resistant pathogens. They are highly selective antimicrobials with low cytotoxicity. The application of natural or bioengineered bacteriocins, along with synergistic combinations of bacteriocins and conventional antibiotics, may be the next step in combating drug-resistant pathogens. This overview focuses on the diversity of lactic acid bacteria and their bacteriocins, but also discusses examples from non-lactic acid bacteria, archaea, and eukaryotes. The therapeutic properties, bioengineering, and combined applications of bacteriocins with conventional antibiotics are evaluated for their potential in human and veterinary medicine.
CRITICAL REVIEWS IN MICROBIOLOGY
(2023)
Article
Biochemical Research Methods
Si Chen, Lihua Fan, Jun Song, Hong Zhang, Leslie Campbell
Summary: In this study, changes in intracellular proteins of Lactococcus spp. 7.17 producing bacteriocin-like substance (BLS) were investigated using quantitative proteomic analysis. A total of 1053 proteins in vegetable medium and 1113 proteins in fruit medium were identified and quantified. Proteins involved in stress responses, DNA processing, metabolism, and biosynthesis were found to be increased in abundance. Key proteins related to BLS production were also identified.
JOURNAL OF PROTEOMICS
(2023)
Review
Food Science & Technology
Svetoslav Dimitrov Todorov, Igor Popov, Richard Weeks, Michael Leonidas Chikindas
Summary: This review critically revisits various research approaches for the application of beneficial organisms and bacteriocins in the food industry. Most studies propose potential applications of these microorganisms and bacteriocins as biopreservatives, but few have evaluated the possible adverse effects of bacteriocins.
Article
Microbiology
Javier Feito, Diogo Contente, Manuel Ponce-Alonso, Lara Diaz-Formoso, Carlos Araujo, Nuria Pena, Juan Borrero, Beatriz Gomez-Sala, Rosa del Campo, Estefania Munoz-Atienza, Pablo E. Hernandez, Luis M. Cintas
Summary: Lactococcus lactis subsp. cremoris WA2-67 is a strain with multiple probiotic properties, including strong antimicrobial activity against fish pathogens, resistance to different environmental conditions, and absence of harmful genes and antibiotic resistance. These findings support its use as a probiotic in aquaculture.
Article
Microbiology
Dengwei Zhang, Jian Zhang, Shanthini Kalimuthu, Jing Liu, Zhi-Man Song, Bei-bei He, Peiyan Cai, Zheng Zhong, Chenchen Feng, Prasanna Neelakantan, Yong-Xin Li
Summary: This study systematically investigates the biosynthetic potential of lactic acid bacteria (LAB) and their profiles in the human microbiome, linking them to their antagonistic contributions to microbiome homeostasis. The research discovers diverse and prevalent antibacterial secondary metabolites produced by LAB, suggesting their potential therapeutic applications.
Article
Biotechnology & Applied Microbiology
Fanqiang Meng, Haizhen Zhao, Ting Nie, Fengxia Lu, Chong Zhang, Yingjian Lu, Zhaoxin Lu
Summary: This study confirmed that acetate activates three histidine kinases, leading to an upregulation of bacteriocin synthesis both in vitro and in vivo. The increased yield of bacteriocins reduces pathogens and increases probiotics in dairy products. The activation of bacteriocin synthesis by acetate may have broad applications in the preservation of dairy products.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Review
Agriculture, Dairy & Animal Science
Juan Carlos Hernandez-Gonzalez, Abigail Martinez-Tapia, Gebim Lazcano-Hernandez, Blanca Estela Garcia-Perez, Nayeli Shantal Castrejon-Jimenez
Summary: Bacteriocins are powerful antimicrobial peptides that have great potential in preventing and treating animal diseases, with lower propensity for resistance development. Their application in animal production and veterinary medicine has shown promising advancements, serving as antimicrobials, probiotics, and immunomodulators.
Article
Biotechnology & Applied Microbiology
Ronaldo Rwubuzizi, Kayque Ordonho Carneiro, Wilhelm Heinrich Holzapfel, Manuela Vaz-Velho, Svetoslav Dimitrov Todorov
Summary: Nine bacteria strains were isolated from home-made fermented vegetables in Northwest Bulgaria, and their identification and characterization were conducted through biochemical, physiological, and biomolecular analyses. These strains were found to produce bacteriocins, which exhibited inhibitory effects on different strains of Listeria spp., Enterococcus spp. (including vancomycin-resistant enterococci), and Staphylococcus spp.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Food Science & Technology
Ana Ruiz de la Bastida, Angela Peiroten, Susana Langa, Juan Luis Arques, Jose Maria Landete
Summary: Isoflavones in soybeans can be transformed into equol and 5-hydroxy-equol by intestinal microbiota, with certain lactic acid bacteria strains being able to enhance their production. Among these, L. fermentum strains have shown particular effectiveness in producing these compounds.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Microbiology
Jorge Enrique Vazquez Bucheli, Joanna Ivy Irorita Fugaban, Wilhelm Heinrich Holzapfel, Svetoslav Dimitrov Todorov
Summary: Antibiotics have been a crucial discovery in medical microbiology, but the rise of antibiotic resistance poses a dangerous threat, driving the demand for new antimicrobials. Bacteriocins, as a type of antimicrobial peptides, can be a potential alternative to conventional antibiotics, especially in controlling resistant pathogens like VRE. The study suggests that bacteriocins show promise in inhibiting VRE strains and could be used as a novel approach to combat antibiotic-resistant infections.
Article
Biochemistry & Molecular Biology
Peijun Tian, Ying Chen, Xin Qian, Renying Zou, Huiyue Zhu, Jianxin Zhao, Hao Zhang, Gang Wang, Wei Chen
Summary: The Pediococcus acidilactici strain CCFM6432 has shown psychobiotic potential by improving gut microbial composition, regulating anxiety-like behaviors, and reducing HPA axis hyperactivity. Its key compound for antimicrobial activity was confirmed to be lactic acid, paving the way for potential mental health management during daily life, especially amid the COVID-19 pandemic.
Article
Pediatrics
Mari Hagbo, Anuradha Ravi, Inga Leena Angell, Marianne Sunde, Jane Ludvigsen, Dzung B. Diep, Steven L. Foley, Maximo Vento, Maria Carmen Collado, Gaspar Perez-Martinez, Knut Rudi
PEDIATRIC RESEARCH
(2020)
Review
Engineering, Biomedical
Raj Kumar Thapa, Dzung B. Diep, Hanne Hjorth Tonnesen
ACTA BIOMATERIALIA
(2020)
Article
Multidisciplinary Sciences
Cristina Campanero, Estefania Munoz-Atienza, Dzung B. Diep, Javier Feito, Sara Arbulu, Rosa del Campo, Ingolf F. Nes, Pablo E. Hernandez, Carmen Herranz, Luis M. Cintas
Article
Pharmacology & Pharmacy
Raj Kumar Thapa, Hanne Cecilie Winther-Larsen, Dzung B. Diep, Hanne Hjorth Tonnesen
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2020)
Article
Microbiology
Kirill Ovchinnikov, Christian Kranjec, Tage Thorstensen, Harald Carlsen, Dzung B. Diep
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2020)
Article
Biotechnology & Applied Microbiology
Christian Kranjec, Kirill V. Ovchinnikov, Torstein Gronseth, Kumar Ebineshan, Aparna Srikantam, Dzung B. Diep
NPJ BIOFILMS AND MICROBIOMES
(2020)
Article
Pharmacology & Pharmacy
Raj Kumar Thapa, Hanne Cecilie Winther-Larsen, Dzung B. Diep, Hanne Hjorth Tonnesen
Summary: The study revealed that combinations such as GakA+GakB exhibited the highest photostability, while GakA+GakB+GakC combination enhanced stability under indoor conditions but reduced under outdoor conditions. Adding antioxidants like glutathione or scavengers like trehalose improved the photostability of peptides.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Microbiology
Ingvild Reinseth, Hanne H. Tonnesen, Harald Carlsen, Dzung B. Diep
Summary: The membrane-bound protease Eep is a crucial virulence factor in pathogenic enterococci, involved in stress response and serving as a receptor for bacteriocins. The bacteriocins have potential for treating VRE infections due to their dual function targeting wildtype enterococcal cells and reducing virulence in bacteriocin-resistant mutants.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Infectious Diseases
Christian Kranjec, Danae Morales Angeles, Marita Torrissen Marli, Lucia Fernandez, Pilar Garcia, Morten Kjos, Dzung B. Diep
Summary: This article outlines the mechanisms by which staphylococci form biofilms and the challenges in eradicating them, highlighting the need for new treatment strategies. It also explores promising therapeutic approaches such as bacteriocins and phage-derived anti-biofilm methods.
Article
Microbiology
Aleksandra Tymoszewska, Kirill Ovchinnikov, Dzung B. Diep, Malgorzata Slodownik, Edyta Maron, Beatriz Martinez, Tamara Aleksandrzak-Piekarczyk
Summary: Resistance of Lactococcus lactis to aureocin A53- and enterocin L50-like bacteriocins is associated with mutations in genes encoding ABC transporter and TCS, leading to upregulation of other operons. Protein modification, rather than absence, is critical for the development of resistance to bacteriocins.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Multidisciplinary Sciences
Christian Kranjec, Sofie S. Kristensen, Karolina T. Bartkiewicz, Mikkel Bronner, Jorunn P. Cavanagh, Aparna Srikantam, Geir Mathiesen, Dzung B. Diep
Summary: A novel hybrid bacteriocin H1 showed superior antimicrobial effects against Staphylococcus haemolyticus and high activity towards various strains without strain-dependency. Combination with broad-spectrum bacteriocins micrococcin P1 and garvicin KS effectively eradicated biofilm-associated cells and prevented development of resistant mutants, indicating its potential as a treatment option for S. haemolyticus.
SCIENTIFIC REPORTS
(2021)
Correction
Biotechnology & Applied Microbiology
Ingvild S. Reinseth, Kirill V. Ovchinnikov, Hanne H. Tonnesen, Harald Carlsen, Dzung B. Diep
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2022)
Article
Microbiology
Thomas F. Oftedal, Kirill Ovchinnikov, Kai A. Hestad, Oliver Goldbeck, Davide Porcellato, Judith Narvhus, Christian U. Riedel, Morten Kjos, Dzung B. Diep
Summary: Bovine mastitis infection in dairy cattle poses a significant economic burden globally, prompting the search for alternative treatments to reduce antibiotic usage. A novel bacteriocin, ubericin K, was discovered from a Streptococcus uberis strain in Norwegian bovine raw milk, showing potent antimicrobial activity. Whole-genome sequencing revealed a gene cluster encoding multiple bacteriocins, with ubericin K targeting bacteria causing mastitis by membrane disruption. The study suggests that ubericin K and the methodology employed could potentially aid in reducing antibiotic use in the dairy industry.
MICROBIOLOGY SPECTRUM
(2021)
Article
Microbiology
Zuzana Rosenbergova, Thomas F. Oftedal, Kirill V. Ovchinnikov, Thasanth Thiyagarajah, Martin Rebros, Dzung B. Diep
Summary: Infections caused by multiresistant pathogens have become a major problem in both human and veterinary medicine. A novel two-peptide lantibiotic with broad-spectrum antimicrobial activity against multiresistant Enterococcus faecium has been isolated from Vagococcus fluvialis, offering potential therapeutic options.
MICROBIOLOGY SPECTRUM
(2022)
Review
Pharmacology & Pharmacy
Raj Kumar Thapa, Dzung B. Diep, Hanne Hjorth Tonnesen
Summary: Nanomedicine-based AMP delivery systems are a recent approach for treating bacterial infections, with advantages such as enhancing AMP stability, controlled release, and targetability. Future studies are needed to address issues like nanoparticle instability, short residence time, and toxicity. Rigorous research on AMP loaded nanoparticle preparation, characterization, in vitro and in vivo antimicrobial effects, and toxicities evaluations are essential.
JOURNAL OF PHARMACEUTICAL INVESTIGATION
(2021)