Article
Veterinary Sciences
Robin B. Guevarra, Eun Sol Kim, Jin Ho Cho, Minho Song, Jae Hyoung Cho, Jun Hyung Lee, Hyeri Kim, Sheena Kim, Gi Beom Keum, Chan Ho Lee, Won Tak Cho, Suphot Watthanaphansak, Hyeun Bum Kim
Summary: This study evaluated the effects of probiotics, prebiotics, and their synbiotic combination on gut microbiota in weaned piglets. The results showed that they all improved intestinal health in the piglets by modulating the gut microbiota.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Food Science & Technology
Chao Liao, Luxin Wang
Summary: The study analyzed the native bacterial populations in three brands of Spring Mix salads and their interaction with Escherichia coli O157:H7 (ECO157) during storage at 4 degrees C. Results showed that the brand factor significantly impacted the diversity and abundance of bacterial populations, while inoculation of ECO157 had a significant effect on the relative abundance and diversity of bacterial populations.
Article
Public, Environmental & Occupational Health
Dmitriy V. Volokhov, Lauren R. Lock, Kristin E. Dyer, Isabella K. DeAnglis, Benjamin R. Andrews, Molly C. Simonis, Sebastian Stockmaier, Gerald G. Carter, Cynthia J. Downs, M. Brock Fenton, Nancy B. Simmons, Daniel J. Becker
Summary: This study uncovered additional hemoplasma diversity in bats by targeting rare or undersampled host species.
Article
Multidisciplinary Sciences
Nimra Khalid, Syed Mohsin Bukhari, Mohammad Y. Alshahrani, Khalil Ur Rehman, Shahbaz Ahmad, Shahla Andleeb, Arshad Javid, Sheikh Muhammad Azam
Summary: The aim of this study was to examine the prevalence and seasonal effect of Escherichia coli in captive avian species, as well as analyze its nucleotide sequences. The research found that regardless of bird species, the prevalence of E. coli in fecal samples was similar, but the incidence of E. coli varied significantly depending on the season in the case of turkey and pheasants, and this impact was statistically significant.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2023)
Article
Veterinary Sciences
Lica Lozica, Maja Mauric Maljkovic, Marin Mazic, Zeljko Gottstein
Summary: Kurthia gibsonii was isolated from 10 unrelated laying hen farms, and coinfection with E. coli and other avian pathogens was observed. Phylogenetic analysis based on the 16S rRNA gene sequences showed some variability among the strains from the same farm, but overall phylogenetic diversity was low. Results from embryo lethality assay indicated that K. gibsonii cannot cause primary infection and may act as an opportunistic pathogen for poultry.
Article
Pediatrics
Xueli Zhang, Shujuan Zeng, Guoqiang Cheng, Liufang He, Mingqiu Chen, Mingbang Wang, Wenhao Zhou, Huixian Qiu, Zhangxing Wang
Summary: This study found that the diversity of gut microbiota and the relative abundance of probiotic Lactobacillus were significantly decreased in the neonatal jaundice group, while potentially harmful gut bacteria such as Escherichia coli and Staphylococcus were significantly increased. The gut microbiota showed a positive correlation with clinical indicators, suggesting that gut microbiota composition may be a potential biomarker for neonatal jaundice.
Article
Agriculture, Dairy & Animal Science
Jixuan Ye, Huaao Yang, Weidong Hu, Keyi Tang, Anfang Liu, Shicheng Bi
Summary: A previous study identified genes and metabolites associated with amino acid metabolism, glycerophospholipid metabolism, and inflammatory response in the liver of broilers with immune stress. The present research aimed to investigate the effect of immune stress on the cecal microbiome in broilers. The results showed that immune stress significantly changed microbiota composition at different taxonomic levels and identified potential microbiota involvement in growth depression mediated by immune stress.
Article
Environmental Sciences
Adriana Osinska, Ewa Korzeniewska, Agnieszka Korzeniowska-Kowal, Anna Wzorek, Monika Harnisz, Piotr Jachimowicz, Martyna Buta-Hubeny, Wiktor Zielinski
Summary: This study aimed to determine the applicability of various methods for identifying E. coli strains in environmental samples. The results of gene sequencing and analysis could help in the selection of more accurate and reliable methods for the preliminary screening and precise identification of E. coli isolated from environmental samples.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Microbiology
Mohamed Rhouma, Charlotte Braley, William Theriault, Alexandre Thibodeau, Sylvain Quessy, Philippe Fravalo
Summary: The study revealed that the ETEC:F4 strain challenge significantly affected the diversity and composition of piglet fecal microbiota, while treatment with colistin sulfate only reduced the populations of E. coli/Shigella during the treatment period.
Article
Veterinary Sciences
Jingyi Sun, Weihao Chen, Zehu Yuan
Summary: This study investigated the intestinal microbiota in lambs with different susceptibilities to E. coli F17 and found that lambs with higher susceptibility had lower bacterial richness and diversity, as well as differences in the abundance of specific bacteria compared to resistant lambs. The Bacteroidetes:Firmicutes ratio was identified as a potential bacterial biomarker for E. coli F17 infection.
VETERINARY SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Ruijuan Gao, Chunjie Wang, Aricha Han, Yanping Tian, Shunan Ren, Wenting Lv, Aorigele Chen, Jian Zhang
Summary: Emodin protects against intestinal damage induced by E. coli O-1 and improves intestinal mucosal barrier function in mice by altering intestinal microbiota composition, increasing beneficial bacteria, and inhibiting harmful bacteria, thereby alleviating diarrhea.
Article
Nutrition & Dietetics
Jianghui Zhang, Yuan Wang, Guimei Chen, Hongli Wang, Liang Sun, Dongmei Zhang, Fangbiao Tao, Zhihua Zhang, Linsheng Yang
Summary: This study on older Chinese adults found associations between individual essential metal(loid)s (EMs) and the compositions of the gut microbiota. The analysis of urine samples and 16S rRNA gene sequencing revealed significant associations between certain EMs and the gut microbiota, particularly in specific populations, suggesting that EMs play an important role in maintaining gut microbiota homeostasis.
Article
Ecology
Robert Starke, Victor Satler Pylro, Daniel Kumazawa Morais
Summary: Sequencing 16S rRNA gene amplicons is commonly used to study prokaryotic communities, but gene copy normalization (GCN) does not improve the accuracy of results. The study questions the use of GCN for metataxonomic surveys until a more comprehensive catalogue of copy numbers becomes available.
Article
Plant Sciences
Maria C. Della Lucia, Giovanni Bertoldo, Chiara Broccanello, Laura Maretto, Samathmika Ravi, Francesco Marinello, Luigi Sartori, Giovanni Marsilio, Andrea Baglieri, Alessandro Romano, Mauro Colombo, Francesco Magro, Giovanni Campagna, Giuseppe Concheri, Andrea Squartini, Piergiorgio Stevanato
Summary: The study found that foliar application of leonardite-based product can significantly increase the abundance of endophytic bacteria with growth-promoting activity, the expression level of genes related to auxin transport proteins, and sugar yield in sugar beet plants.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Chemistry, Medicinal
Yu Ma, Qian Zhang, Wenqiang Liu, Zhaohua Chen, Chao Zou, Linglin Fu, Yanbo Wang, Yixiang Liu
Summary: DESP can prevent bacterial diarrhea by regulating intestinal flora, as shown by improvements in inflammation-related factors and increased richness and diversity of the intestinal microbiota, specifically with increased Firmicutes and Actinobacteria and decreased Proteobacteria.
Article
Biochemistry & Molecular Biology
Keiichi Izumikawa, Yuko Nobe, Hideaki Ishikawa, Yoshio Yamauchi, Masato Taoka, Ko Sato, Hiroshi Nakayama, Richard J. Simpson, Toshiaki Isobe, Nobuhiro Takahashi
NUCLEIC ACIDS RESEARCH
(2019)
Article
Plant Sciences
Hiromi Suzuki, Tomokazu Koshiba, Chiharu Fujita, Yoshio Yamauchi, Taro Kimura, Toshiaki Isobe, Tatsuya Sakai, Masato Taoka, Takashi Okamoto
JOURNAL OF EXPERIMENTAL BOTANY
(2019)
Article
Multidisciplinary Sciences
Yae Kurosawa, Shigehiro Sato, Tsuneo Okuyama, Masato Taoka
Article
Multidisciplinary Sciences
Steffi Jimmy, Chayan Kumar Saha, Tatsuaki Kurata, Constantine Stavropoulos, Sofia Raquel Alves Oliveira, Alan Koh, Albinas Cepauskas, Hiraku Takada, Dominik Rejman, Tanel Tenson, Henrik Strahl, Abel Garcia-Pino, Vasili Hauryliuk, Gemma C. Atkinson
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Chemistry, Analytical
Yuka Yamaki, Yuko Nobe, Masami Koike, Yoshio Yamauchi, Kouji Hirota, Nobuhiro Takahashi, Hiroshi Nakayama, Toshiaki Isobe, Masato Taoka
ANALYTICAL CHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Aldema Sas-Chen, Justin M. Thomas, Donna Matzov, Masato Taoka, Kellie D. Nance, Ronit Nir, Keri M. Bryson, Ran Shachar, Geraldy L. S. Liman, Brett W. Burkhart, Supuni Thalalla Gamage, Yuko Nobe, Chloe A. Briney, Michaella J. Levy, Ryan T. Fuchs, G. Brett Robb, Jesse Hartmann, Sunny Sharma, Qishan Lin, Laurence Florens, Michael P. Washburn, Toshiaki Isobe, Thomas J. Santangelo, Moran Shalev-Benami, Jordan L. Meier, Schraga Schwartz
Article
Biochemistry & Molecular Biology
Donna Matzov, Masato Taoka, Yuko Nobe, Yoshio Yamauchi, Yehuda Halfon, Nofar Asis, Ella Zimermann, Haim Rozenberg, Anat Bashan, Shashi Bhushan, Toshiaki Isobe, Michael W. Gray, Ada Yonath, Moran Shalev-Benami
NUCLEIC ACIDS RESEARCH
(2020)
Article
Oncology
Ania Naila Guerrieri, Federico Zacchini, Carmine Onofrillo, Sara Di Viggiano, Marianna Penzo, Alessio Ansuini, Ilaria Gandin, Yuko Nobe, Masato Taoka, Toshiaki Isobe, Davide Trere, Lorenzo Montanaro
Article
Multidisciplinary Sciences
Takafumi Tozawa, Kohichi Matsunaga, Tetsuro Izumi, Naotake Shigehisa, Takamasa Uekita, Masato Taoka, Tohru Ichimura
Summary: In this study, we investigated the formation of cytoplasmic bodies (CBs) and found that they exist in a liquid droplet state through liquid-liquid phase separation (LLPS). The CB droplets contain various endogenous proteins and enzymes, including ubiquitin. Furthermore, we demonstrated that the activation of the intracellular ubiquitination cascade promotes the assembly of TRIM proteins into CBs. These findings provide a new mechanical basis for understanding the organization of CBs.
Article
Chemistry, Analytical
Hiroshi Nakayama, Yuko Nobe, Masami Koike, Masato Taoka
Summary: Researchers developed an analytical platform based on LC-MS that can quantitatively characterize long, modified mRNAs and confirm their primary structures and quality by comparing them to a stable isotope-labeled reference. This platform is valuable for analyzing mRNA vaccines and other mRNA medicines.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Hiroki Hamaguchi, Kitora Dohi, Takaomi Sakai, Masato Taoka, Toshiaki Isobe, Tsubasa S. Matsui, Shinji Deguchi, Yasuro Furuichi, Nobuharu L. Fujii, Yasuko Manabe
Summary: Myokines, such as platelet-derived growth factor sub-unit B (PDGF-B), play a crucial role in regulating muscle mass and function by acting locally on muscle cells or satellite cells. This study found that PDGF-B is continuously secreted by muscle cells and its secretion increases during myoblast to myotube differentiation. Treatment with PDGF-B enhances myoblast proliferation and promotes myotube maturation, as evidenced by increased myotube diameter, myosin heavy chain expression, and contractile force. These findings suggest that PDGF-B acts as a paracrine or autocrine myokine to contribute to skeletal muscle regeneration.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Yoriko Watanabe, Yuko Nobe, Masato Taoka, Takashi Okamoto
Summary: The presence of feeder cells and 2,4-D in the culture medium has significant effects on the growth and development of rice zygotes. Removal of 2,4-D decreases the division rates of multicellular embryos, while removal of feeder cells results in defective karyogamy. Using a conditioned medium can correct developmental disorders. Hydrolytic enzymes released by feeder cells, including alpha-amylase, are suggested to be involved in zygotic development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Masato Taoka, Kota Kamei, Akiko Kashima, Yuko Nobe, Takahiro Takekiyo, Takamasa Uekita, Tohru Ichimura
Summary: Many ion channels and receptor proteins are potential targets for new drugs. This study presents a novel proteomic sample preparation platform that allows for the processing of sub-microgram level starting materials using an ionic liquid-based sample preparation strategy and an in-StageTip technique. This approach enables the rapid solubilization and analysis of integral membrane proteins from human samples, surpassing the performance of conventional methods.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Patrick Cottilli, Yuzuru Itoh, Yuko Nobe, Anton S. Petrov, Purificacion Lison, Masato Taoka, Alexey Amunts
Summary: Protein synthesis in crop plants plays a crucial role in balancing food and fuel, affecting human metabolism and lifespan. This study reveals the structure and modifications of a plant ribosome, providing a reference model for further studies.
PLANT COMMUNICATIONS
(2022)
Article
Cell Biology
Yuichi Wakana, Kaito Hayashi, Takumi Nemoto, Chiaki Watanabe, Masato Taoka, Jessica Angulo-Capel, Maria F. Garcia-Parajo, Hidetoshi Kumata, Tomonari Umemura, Hiroki Inoue, Kohei Arasaki, Felix Campelo, Mitsuo Tagaya
Summary: The study reveals that in cholesterol-fed cells, SCAP interacts with the VAP-OSBP complex at ER-Golgi membrane contact sites to facilitate CARTS biogenesis. Knockdown of SCAP inhibits PI4P turnover and alters the subcellular distribution of the VAP-OSBP complex, ultimately leading to decreased CARTS formation.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ankita Chadda, Alexander G. Kozlov, Binh Nguyen, Timothy M. Lohman, Eric A. Galburt
Summary: In this study, it was found that the DNA damage response in Mycobacterium tuberculosis differs from well-studied model bacteria. The DNA repair helicase UvrD1 in Mtb is activated through a redox-dependent process and is closely associated with the homo-dimeric Ku protein. Additionally, Ku protein is shown to stimulate the helicase activity of UvrD1.
JOURNAL OF MOLECULAR BIOLOGY
(2024)