Article
Multidisciplinary Sciences
Nanaka Kaneshiro, Masato Komai, Ryosuke Imaoka, Atsuya Ikeda, Yuji Kamikubo, Takashi Saito, Takaomi C. Saido, Taisuke Tomita, Tadafumi Hashimoto, Takeshi Iwatsubo, Takashi Sakurai, Takashi Uehara, Nobumasa Takasugi
Summary: This study investigates the pathological mechanisms of vesicular traffic impairment in Alzheimer's disease and proposes a therapeutic target for AD.
Article
Multidisciplinary Sciences
Andrew R. Castle, Sang-Gyun Kang, Ghazaleh Eskandari-Sedighi, Serene Wohlgemuth, My-Anh Nguyen, Daniel J. Drucker, Erin E. Mulvihill, David Westaway
Summary: In prion infections, the cellular prion protein (PrPC) can convert to pathogenic conformations (PrPSc) and bind toxic oligomers formed by amyloid-beta alpha-synuclein and tau. β-Endoproteolysis of PrPC into N- and C-terminal fragments (N2 and C2) is of interest, but the proteolytic mechanism remains unconfirmed. A recent study identified S9B peptidase subfamily membrane proteins as potential β-cleavage candidates for PrPC.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Biochemistry & Molecular Biology
Lucia Gallego Villarejo, Lisa Bachmann, David Marks, Maite Brachthaeuser, Alexander Geidies, Thorsten Mueller
Summary: Intracellular amyloid beta (iAβ) plays a crucial role in neurodegeneration and serves as a pathological marker. Modulating iAβ through pharmacological treatment has shown beneficial effects on cognitive properties. Future research should focus on the impact of viral infections on iAβ generation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Fisheries
Wenji Wang, Suying Hou, Guoquan Chen, Liqun Xia, Jianlin Chen, Zhiwen Wang, Yishan Lu
Summary: A glutamic endopeptidase homolog from Nocardia seriolae (GluNS) was cloned and its function was elucidated. Overexpression of GluNS induced apoptotic characteristics in FHM cells, providing new insights into the function of glutamic endopeptidase from N. seriolae.
JOURNAL OF FISH DISEASES
(2021)
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Biochemistry & Molecular Biology
Adriana Maria Diaz-Anaya, Louise Gerard, Martine Albert, Jean-Francois Gaussin, Marielle Boonen, Jean-Pierre Gillet
Summary: ABCB5 beta is a marker protein associated with melanoma stem cells and skin progenitor cells. The study reveals that ABCB5 beta is predominantly localized to the endoplasmic reticulum and is degraded by the proteasome system. Treatment with SAHA, a molecule promoting chaperone-assisted folding, increases the expression of ABCB5 beta.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Chyi Wei Chung, Amberley D. Stephens, Tasuku Konno, Edward Ward, Edward Avezov, Clemens F. Kaminski, Ali A. Hassanali, Gabriele S. Kaminski Schierle
Summary: This study demonstrates that aggregation of A beta 42 in live cells leads to an increase in cell-averaged temperatures, which can be mitigated by treatment with an aggregation inhibitor. It also suggests that under intracellular-mimicking ionic conditions, aggregation and heat retention by A beta peptides are favored, potentially promoting thermogenesis.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Cell Biology
Magdalena Antonino, Paula Marmo, Carlos Leandro Freites, Gonzalo Emiliano Quassollo, Maria Florencia Sanchez, Alfredo Lorenzo, Elena Anahi Bignante
Summary: Alzheimer's disease (AD) is characterized by the accumulation of amyloid beta (Aβ) in the brain, which is produced by the cleavage of amyloid precursor protein (APP). Aβ assemblies enhance the interaction between APP and BACE1, leading to increased production and accumulation of Aβ. This process involves Aβ-APP/Go signaling and Gβγ subunit signaling.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Jianbin Zhang, Daixi Jiang, Shuangzhe Lin, Yuqing Cheng, Jiaxing Pan, Wenjin Ding, Yuanwen Chen, Jiangao Fan
Summary: The study found that upregulated expression of PREP in the MCD-induced NASH model, and disruption of PREP alleviated MCD-induced hepatic inflammation and improved oxidative stress status, primarily mediated by the activation of P53 and its downstream signaling pathways.
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Genetics & Heredity
Jiancheng Sun, Feng Qin, Fuzhen Sun, Ping He, Erjun Wei, Runpeng Wang, Feng Zhu, Qiang Wang, Xudong Tang, Yiling Zhang, Zhongyuan Shen
Summary: In this study, the NbSec61 alpha and NbSec61 gamma genes were identified in the microsporidian Nosema bombycis for the first time. These genes were found to play important roles in the transport across the endoplasmic reticulum and intracellular development of N. bombycis.
Article
Biochemical Research Methods
Zhao-Yue Zhang, Lin Ning, Xiucai Ye, Yu-He Yang, Yasunori Futamura, Tetsuya Sakurai, Hao Lin
Summary: The location of miRNAs in cells plays a crucial role in their regulatory function. Current prediction algorithms for miRNA subcellular localization have limitations. In this study, a new data partitioning strategy and deep learning algorithm were proposed to accurately predict miRNA subcellular localization and explore the underlying mechanisms through motif analysis. Additionally, a user-friendly web server was established for convenient use.
BRIEFINGS IN BIOINFORMATICS
(2022)
Article
Cell Biology
Eduardo J. Fernandez-Perez, Braulio Munoz, Denisse A. Bascunan, Christian Peters, Nicolas O. Riffo-Lepe, Maria P. Espinoza, Peter J. Morgan, Caroline Filippi, Romain Bourboulou, Urmi Sengupta, Rakez Kayed, Jerome Epsztein, Luis G. Aguayo
Summary: In early stages of Alzheimer’s disease, intracellular amyloid beta oligomers increase neuronal excitability through PKC-dependent mechanisms, by enhancing synaptic current frequency and potentiation of AMPA receptor-mediated current. Additionally, nitric oxide-mediated retrograde signaling is involved in the increased excitability, suggesting a spread of hyperexcitability through synaptic-driven mechanisms.
Article
Agriculture, Multidisciplinary
Pei Shan, Chi-Tang Ho, Lei Zhang, Xianli Gao, Hong Lin, Ting Xu, Bo Wang, Jiangyan Fu, Ronghai He, Yaqiong Zhang
Summary: This study isolated and identified an APE from Aspergillus niger that can degrade approximately 50% of the B3 subunit and increase proline content in soy sauce. APE is an acidic salt-tolerant serine protease that is optimally active at 40 degrees C and pH 4.0. High salinity affects the spatial structures and catalytic activity of APE, as well as the rigidity of the B3 subunit.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Fei Li, Yunlu Wei, Jing Zhao, Guoyong Yu, Linlin Huang, Quanhong Li
Summary: The study investigated the transmembrane transport and subcellular localization of pumpkin polysaccharides, providing insights into their bioactivities and mechanisms of action.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Analytical
Adukkadan N. Ramya, Manu M. Joseph, Varsha Karunakaran, Chekrain Valappil Shihas Ahammed, Animesh Samanta, Kaustabh K. Maiti
Summary: Hydrogen sulfide (H2S), an essential neurotransmitter, plays a role in regulating brain function. This study presents a molecular probe, TPE-NBD-D, that can sense and visualize the disaggregation of A13 caused by H2S, showing potential therapeutic benefits for Alzheimer's disease.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Plant Sciences
Xinglong Su, Fei Meng, Yingying Liu, Weimin Jiang, Zhaojian Wang, Liping Wu, Xiaohu Guo, Xiaoyan Yao, Jing Wu, Zongping Sun, Liangping Zha, Shuangying Gui, Daiyin Peng, Shihai Xing
Summary: This study identified a beta-glucosidase in Platycodon grandiflorus that can convert platycoside E to platycodin D. The expression level of the enzyme was higher at night than during the day and was regulated by light. These findings are important for further studying the biosynthesis and metabolism regulation of triterpenoid saponins in P. grandiflorus.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Anatomy & Morphology
Andrea Hunyadi, Botond Gaal, Clara Matesz, Zoltan Meszar, Markus Morawski, Katja Reimann, David Lendvai, Alan Alpar, Ildiko Weber, Eva Racz
BRAIN STRUCTURE & FUNCTION
(2020)
Article
Biochemistry & Molecular Biology
Geoffrey J. Eill, Ashis Sinha, Markus Morawskr, Mariano S. Viapiano, Russell T. Matthews
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Janett Koeppen, Anja Schulze, Lisa Machner, Michael Wermann, Rico Eichentopf, Max Guthardt, Angelika Haehnel, Jessica Klehm, Marie-Christin Kriegeskorte, Maike Hartlage-Ruebsamen, Markus Morawski, Stephan von Hoersten, Hans-Ulrich Demuth, Steffen Rossner, Stephan Schilling
Article
Neurosciences
Sophie Schmidt, Caroline Stapf, Sandra Schmutzler, Ingolf Lachmann, Thomas Arendt, Max Holzer, Mandy Sonntag, Markus Morawski
Summary: Selected neurons in the central nervous system are surrounded by perineuronal nets (PNs) which regulate plasticity and synaptic physiology. Research showed that aggrecan, a component of PNs, has a neuroprotective role by preventing internalization of pathological tau forms. However, in a mouse model, reduced aggrecan levels led to increased tau protein and phosphorylation, suggesting a correlation between aggrecan and tau pathology triggered by mutation.
EUROPEAN JOURNAL OF NEUROSCIENCE
(2021)
Article
Multidisciplinary Sciences
Evgeniya Kirilina, Saskia Helbling, Markus Morawski, Kerrin Pine, Katja Reimann, Steffen Jankuhn, Juliane Dinse, Andreas Deistung, Jurgen R. Reichenbach, Robert Trampel, Stefan Geyer, Larissa Mueller, Norbert Jakubowski, Thomas Arendt, Pierre-Louis Bazin, Nikolaus Weiskopf
Article
Oncology
Hannes Borchardt, Alexander Ewe, Markus Morawski, Ulrike Weirauch, Achim Aigner
Summary: The replacement of miR24-3p may be an effective strategy for pancreatic cancer therapy, leading to cell inhibition through the induction of apoptosis, autophagy, and ROS. Comprehensive analyses revealed the transcriptional regulation of several important oncogenes and oncogenic pathways by miR24-3p on the molecular level.
Editorial Material
Neurosciences
Alexander Dityatev, Constanze Seidenbecher, Markus Morawski
EUROPEAN JOURNAL OF NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Anja Reinert, Tilo Reinert, Thomas Arendt, Markus Morawski
Summary: A subpopulation of neurons, characterized by a special extracellular matrix composition forming a perineuronal net (PN) with a high affinity to iron, is less vulnerable to iron-induced oxidative stress and neurodegeneration. These PN+ neurons have higher intracellular iron concentrations and increased levels of iron proteins, with the iron concentration of the PN itself not being increased. The properties of these resilient pacemaker neurons with bustling iron metabolism and outstanding iron handling capabilities contribute to their low vulnerability to iron-induced oxidative stress and degeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Henriette Rusch, Malte Brammerloh, Jens Stieler, Mandy Sonntag, Siawoosh Mohammadi, Nikolaus Weiskopf, Thomas Arendt, Evgeniya Kirilina, Markus Morawski
Summary: The accessibility of new wide-scale multimodal imaging techniques has led to the emergence of numerous tissue clearing techniques. This study compares seven different tissue clearing approaches for aged human brain tissue blocks (>5 mm) to identify the most practical and efficient method. The results show that combining the CLARITY or iDISCO techniques with specific imaging setups is the best approach for clearing aged human brain tissue and generating 3D microscopic images.
Article
Cell Biology
Fyn Kaiser, Markus Morawski, Knut Krohn, Nada Rayes, Cheng-Chih Hsiao, Marianne Quaas, Gabriela Aust
Summary: GPR56, also known as gene ADGRG1, plays significant roles in various physiological processes and shows broad expression in human tissues, especially in microglia in the adult brain and cuboidal secreting epithelia in peripheral tissues. High levels of ADGRG1 mRNA in specific tissues correlate with elevated levels of GPR56 protein expression in corresponding cell types.
Article
Neurosciences
Laurin Mordhorst, Maria Morozova, Sebastian Papazoglou, Bjorn Fricke, Jan Malte Oeschger, Thibault Tabarin, Henriette Rusch, Carsten Jaeger, Stefan Geyer, Nikolaus Weiskopf, Markus Morawski, Siawoosh Mohammadi
Summary: Non-invasive assessment of axon radii via MRI is important for clinical and neuroscience research, but lacks representative histological reference data. This study proposes a method using CNN-based segmentation to generate a representative reference for the effective radius (r(eff)) of axons. The results show that r(eff) estimation is more accurate and less biased compared to the traditional method of estimating the arithmetic mean radius (r(arith)).
Article
Biochemistry & Molecular Biology
Sophie Schmidt, Max Holzer, Thomas Arendt, Mandy Sonntag, Markus Morawski
Summary: Tau mutations promote the formation of tau oligomers and filaments, while certain types of neurons are spared of tau pathology due to the protective function of perineuronal nets (PNs), which prevent the internalization of misfolded tau. The study demonstrates a complex interrelation between tau and the proteoglycans of the PN, suggesting that tau can regulate the structure and protein expression of PNs.
Article
Microbiology
Julia Muenzker, Nadine Haase, Andreas Till, Robert Sucher, Sven-Bastiaan Haange, Linda Nemetschke, Thorsten Gnad, Elisabeth Jaeger, Jiesi Chen, Sjaak J. Riede, Rima Chakaroun, Lucas Massier, Peter Kovacs, Mario Ost, Ulrike Rolle-Kampczyk, Nico Jehmlich, Juliane Weiner, John T. Heiker, Nora Kloeting, Gudrun Seeger, Markus Morawski, Verena Keitel, Alexander Pfeifer, Martin von Bergen, Joerg Heeren, Ute Kruegel, Wiebke K. Fenske
Summary: This study demonstrates the significant role of the gut microbiome in the energy and metabolic improvements achieved through gastric bypass surgery. By eliminating and transplanting the gut microbiota, the study identifies a signaling cascade involving altered bile acid receptor signaling that readjusts energy imbalance and metabolic diseases in obese hosts.
Article
Neurosciences
Henrik Marschner, Andre Pampel, Roland Mueller, Katja Reimann, Nicolas Bock, Markus Morawski, Stefan Geyer, Harald E. Moeller
Summary: Cell membranes and macromolecules/paramagnetic compounds interact with water proton spins, providing MRI contrast and tissue composition information. Quantitative magnetization transfer (qMT) parameters were measured at high spatial resolution in a fixed marmoset brain slice and compared to histology results. R1obs and R2* were linearly correlated with iron content, while distinct differences were observed between gray and white matter for qMT parameters and relaxometry. These findings suggest that the water interacting macromolecular pool consists of myelin and non-myelin contributions, and R1obs and R2* are more sensitive for depicting microstructural differences between cortical layers than T.
Meeting Abstract
Dermatology
T. Wippold, K. T. Nguyen, M. Morawski, A. Saalbach, J. Sapudom, T. Pompe, U. Anderegg
EXPERIMENTAL DERMATOLOGY
(2021)
