Article
Fisheries
Xuangang Wang, Xintong Wang, Hengshun Li, Xiangfu Kong, Dianyang Zhou, Qiannan Chen, Quanqi Zhang, Haiyang Yu
Summary: In this study, the antibacterial activity of turbot skin mucus was investigated and found to be effective against both Gram-positive and Gram-negative bacteria. Additionally, biochemical analysis revealed strong antioxidant, protease, and lysozyme activities in the mucus. Proteomic profiling of the mucus before and after Vibrio anguillarum infection identified differentially expressed proteins associated with immunity, biological adhesion, and cell junction, indicating complex changes in the mucus during pathogen infection.
Article
Immunology
Qian Chen, Bangguo Ma, Mingzhe Xu, Huiwen Xu, Zimiao Yan, Fei Wang, Yiran Wang, Zitong Huang, Shuchang Yin, Yancui Zhao, Lei Wang, Hongyan Wu, Xiaoli Liu
Summary: In this study, exosomes were isolated from Vibrio harveyi and Vibrio anguillarum using a supercentrifugation method, and their protein compositions were analyzed. The exosomes of these two vibrios contained virulence factors and also played roles in important bacterial activities. Further experiments confirmed the involvement of exosomes in vibrio toxicity. This study provides a valuable proteome database for understanding the pathogenic mechanisms of vibrios from the exosome perspective.
MICROBIAL PATHOGENESIS
(2023)
Article
Biochemistry & Molecular Biology
Quanxin Gao, Shaokui Yi, Jinping Luo, Qianqian Xing, Jiali Lv, Panhuang Wang, Cuihua Wang, Yang Li
Summary: By knocking out the FlaB gene of Vibrio anguillarum, this study found little impact on the induction of immune-related gene expression, and the mutant displayed similar flagella morphology and immune characteristics to the wild-type strain. This suggests that knockout of FlaB may promote the expression and function of other flagellin proteins.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Fisheries
Na Yang, Fuhang Song, Steven W. Polyak, Jianguo Liu
Summary: The study identified that the resistance mechanism of the V. anguillarum mutant to actinonin is associated with bacterial respiration.
Article
Biology
Francis J. Santoriello, Paul C. Kirchberger, Yann Boucher, Stefan Pukatzki
Summary: Vibrio cholerae uses the type VI secretion system (T6SS) to infect neighboring microbes and establish its niche. Horizontal acquisition of effectors was crucial for the evolution of pandemic V. cholerae. This study suggests that the fish pathogen Vibrio anguillarum may serve as a donor of pandemic-associated V. cholerae T6SS effectors and facilitate competition between pre-pandemic V. cholerae and V. anguillarum for an environmental niche.
LIFE SCIENCE ALLIANCE
(2022)
Article
Fisheries
Yue Wang, Shaowei Zhai, Qijuan Wan, Ming Xu, Minxia Chen, Songlin Guo
Summary: This study first reports the pathogenicity of E. anguillarum to American eels and the hub genes related to host anti-E. anguillarum infection. Histopathological observations and molecular detection reveal changes in bacterial loads in the kidney and liver post E. anguillarum infection.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Xinan Wang, Aijun Ma
Summary: Seven immune factors were measured by PCR in the livers, spleens, and head kidneys of turbot infected with Vibrio anguillarum, and AMMI and GGE analysis were used to investigate the interactions between genotype and tissue on immune factor expression. The results indicated that genotype had a significant impact on immune factor expression compared to tissue effects and genotype x tissue interactions, with head kidney showing the strongest ability to distinguish the seven immune factors.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Xiaoxu Qu, Xia Wang, Bo Liu, Min Chen, Junhao Ning, Haijun Liu, Guilong Liu, Xin Xu, Xiaotong Zhang, Kai Yu, He Xu, Xia Lu, Chunde Wang
Summary: In this study, hybrids between bay scallops and Peruvian scallops exhibited higher resistance to Vibrio bacteria, but the mechanism is unclear. The gene IFI44 was found to play an important role in the immune response to Vibrio in scallops. These findings suggest that IFI44 gene is involved in the higher resistance of hybrid scallops to Vibrio bacteria.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Zhongguo Shan, Ning Guan, Yanpeng Yang, Tong Jin, Xiaodong Xia, Weidong Liu
Summary: Punicalagin (PNG) shows a promising prophylactic and curative potential against Vibrio anguillarum infection in Yesso scallops, by damaging bacterial membrane, reducing swimming ability, and increasing enzyme activities in hemolymph. It can facilitate bacterial clearance and suppress the expression of immune response genes, making it a potential antibacterial drug and immunostimulant for pathogen-infected scallops in aquaculture.
Article
Immunology
Jing-Yue Hu, Xiao-Kai Zhang, Cheng-Qi Xin, Lei Zhang, Jian Kang, Ping Gong
Summary: We report a case of severe V. anguillarum infection in a 70-year-old man from Dalian, China, who had low immunity due to long-term glucocorticoid use. Despite aggressive treatment, the patient died of septic shock and multiple organ dysfunction syndrome. This case highlights the potential for human infection by V. anguillarum, especially in immunocompromised individuals.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Fisheries
Chengbin Gao, Xin Cai, Min Cao, Qiang Fu, Ning Yang, Xiaoli Liu, Beibei Wang, Chao Li
Summary: MicroRNAs play crucial roles in the immune response of turbot, constructing miRNA-mRNA networks and identifying DE genes and miRNAs involved in immune response. Functional analysis revealed the functions and regulatory mechanisms of these target genes, providing valuable information for understanding the regulation mechanisms related to disease resistance in the immune system.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Fisheries
Qian Yang, Xiao-Dong Yang, Meng-Qun Liu, Chu Zeng, Hong-Kui Zhao, Kai-Wen Xiang, Zhi-Shuai Hou, Hai-Shen Wen, Ji-Fang Li
Summary: Rainbow trout, an important cold-water fish, is threatened by viruses and bacteria in the farming industry. Vibrio anguillarum, a common disease-causing pathogen, infects fish mainly through the skin, gills, lateral line, and intestine. In response to the infection, rainbow trout activate immunomodulatory genes and down-regulate tissue function-related genes, while a sustained inflammatory response can lead to tissue damage and death. These findings provide a theoretical basis for breeding rainbow trout with disease resistance.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Jian-Fei Lu, Sheng Luo, Tian-Cheng Jin, Li-Cong Wang, Guan-Jun Yang, Xin-Jiang Lu, Jiong Chen
Summary: The study demonstrated that betaine can improve survival rates, reduce bacterial loads and tissue damage, and decrease inflammatory responses in ayu infected with Vibrio anguillarum under high salinity conditions. Additionally, betaine can enhance phagocytosis activity and bacterial killing capability in monocytes/macrophages. Betaine mediates host defense and modulates antibacterial responses through MO/M43 in Vibrio anguillarum-infected ayu.
Article
Fisheries
Lianfei Song, Xianzhi Dong, Guobin Hu
Summary: This study systematically analyzed the immune response of red sea bream infected with Vibrio anguillarum, providing valuable data support for understanding the immune mechanism and laying the foundation for disease prevention and treatment. Genes were annotated and differentially expressed genes were identified in the head kidney and spleen, with pathways related to antigen processing, phagocytosis, and immune response being significantly enriched.
AQUACULTURE REPORTS
(2021)
Article
Immunology
Zhihui Yin, Hongtao Nie, Kunyin Jiang, Xiwu Yan
Summary: The clam Ruditapes philippinarum is an important species in the marine aquaculture industry in China, but its cultivation has been negatively impacted by bacterial pathogens. In this study, transcriptome libraries of R. philippinarum with different resistance levels against Vibrio anguillarum were constructed and analyzed. Several immune factors responding to V. anguillarum infection were identified, providing insights into the immune response of R. philippinarum. The findings contribute to the understanding of resistant genes and key pathways related to Vibrio challenge in this species.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell & Tissue Engineering
Dongsheng Guo, Feima Wu, Haikun Liu, Ge Gao, Shanglong Kou, Fan Yang, Nasir Abbas, Tiancheng Zhou, Xiujuan Cai, Hui Zhang, Dajiang Qin, Jialiang Li, Kecheng Xu, Yin-xiong Li
STEM CELL RESEARCH
(2017)
Article
Cell & Tissue Engineering
Yingying Xu, Yuhang Wu, Dongsheng Guo, Ge Gao, Keyu Lai, Fan Yang, Kepin Wang, Han Wu, Liangxue Lai, Jialiang Li, Kecheng Xu, Yin-Xiong Li
STEM CELL RESEARCH
(2017)
Article
Cell & Tissue Engineering
Yanli Liu, Feima Wu, Yuanqi Zhuang, Dongsheng Guo, Nasir Abbas, Ruzi Wei, Kepin Wang, Yan Chen, Jiawang Tao, Yuhang Wu, Fang Yuan, Tingcai Pan, Fan Yang, Keyu Lai, Liangxue Lai, Yin-xiong Li
STEM CELL RESEARCH
(2017)
Article
Cell & Tissue Engineering
Yanli Liu, Aynisahan Ruzi, Dongsheng Guo, Feima Wu, Haikun Liu, Han Wu, Yuhang Wu, Fang Yuan, Yuanqi Zhuang, Fan Yang, Keyu Lai, Liangxue Lai, Yin-xiong Li
STEM CELL RESEARCH
(2017)
Article
Cell & Tissue Engineering
Fang Yuan, Dongsheng Guo, Ge Gaob, Yanli Liu, Yingying Xu, Yuhang Wu, Fan Yang, Xinrong Ke, Keyu Lai, Liangqing Hong, Yin-xiong Li
STEM CELL RESEARCH
(2017)
Article
Cell & Tissue Engineering
Dongsheng Guo, Feima Wu, Haikun Liu, Ge Gao, Shanglong Kou, Fan Yang, Nasir Abbas, Tiancheng Zhou, Xiujuan Cai, Hui Zhang, Dajiang Qin, Jialiang Li, Kecheng Xu, Yin-xiong Li
STEM CELL RESEARCH
(2017)
Article
Cell & Tissue Engineering
Dongsheng Guo, Haikun Liu, Ge Gao, Yanli Liu, Yuanqi Zhuang, Fan Yang, Kepin Wang, Tiancheng Zhou, Dajiang Qin, Liangqing Hong, Jialiang Li, Kecheng Xu, Yin-xiong Li
STEM CELL RESEARCH
(2017)
Article
Cell & Tissue Engineering
Guosheng Xu, Dongsheng Guo, Feima Wu, Nasir Abbas, Keyu Lai, Fang Yuan, Kai You, Yanli Liu, Yuanqi Zhuang, Yuhang Wu, Yingying Xu, Yan Chen, Fan Yang, Tingcai Pan, Yin-xiong Li
STEM CELL RESEARCH
(2018)
Article
Cell & Tissue Engineering
Fang Yuan, Dongsheng Guo, Yanli Liu, Yingying Xu, Ge Gao, Yuhang Wu, Fan Yang, Xinrong Ke, Keyu Lai, Hongcheng Wei, Yin-Xiong Li
STEM CELL RESEARCH
(2018)
Article
Cell & Tissue Engineering
Yanli Liu, Feima Wu, Dong Liu, Ruzhi Wei, Nasir Abbas, Jianhong Xia, Dongwei Li, Haiyun Wang, Yuanqi Zhuang, Dongsheng Guo, Yan Chen, Yuhang Wu, Xinrong Ke, Jiawang Tao, Fan Yang, Keyu Lai, Xiaodong Shu, Yin-xiong Li
STEM CELL RESEARCH
(2018)
Article
Multidisciplinary Sciences
Haikun Liu, Dongsheng Guo, Aynisahan Ruzi, Yan Chen, Tingcai Pan, Fan Yang, Jialiang Li, Kecheng Xu, Tiancheng Zhou, Dajiang Qin, Yin-xiong Li
Article
Multidisciplinary Sciences
Sukanya Iyer, Sneha Suresh, Dongsheng Guo, Katelyn Daman, Jennifer C. J. Chen, Pengpeng Liu, Marina Zieger, Kevin Luk, Benjamin P. Roscoe, Christian Mueller, Oliver D. King, Charles P. Emerson, Scot A. Wolfe
Article
Multidisciplinary Sciences
Guosheng Xu, Feima Wu, Xiaotong Gu, Jiaye Zhang, Kai You, Yan Chen, Anteneh Getachew, Yuanqi Zhuang, Xiaofen Zhong, Zuoxian Lin, Dongsheng Guo, Fan Yang, Tingcai Pan, Hongcheng Wei, Yin-xiong Li
SCIENTIFIC REPORTS
(2019)
Article
Biology
Dongsheng Guo, Katelyn Daman, Jennifer J. C. Chen, Meng-Jiao Shi, Jing Yan, Zdenka Matijasevic, Amanda M. Rickard, Monica H. Bennett, Alex Kiselyov, Haowen Zhou, Anne G. Bang, Kathryn R. Wagner, Rene Maehr, Oliver D. King, Lawrence J. Hayward, Charles P. Emerson
Summary: Researchers have successfully generated skeletal muscle myoblasts from human induced pluripotent stem cells using an efficient and reliable transgene-free protocol. These myoblasts can be stably propagated and have the ability to differentiate into myotubes and regenerate reserve cells. In addition, they exhibit stem cell-like properties when engrafted into mouse muscle and are responsive to signals in the host muscle. This study demonstrates the utility of these myoblasts for investigating human myogenesis and disease pathogenesis, as well as for developing muscle stem cell therapeutics.
Article
Cell Biology
Ziqi Cheng, Dongsheng Guo, Aynisahan Ruzi, Tingcai Pan, Kai You, Yan Chen, Xinping Huang, Jiaye Zhang, Fan Yang, Lizhi Niu, Kecheng Xu, Yin-Xiong Li
Summary: This study utilized patient-origin iPSCs to model multiple phenotypes of MEN1, revealing an association between increased insulin-positive cells in MEN1-iPSC derived cells and elevated expression of various factors and signaling pathways, and suggesting GLP-1R as a potential therapeutic target for MEN1-related hyperinsulinemia.