Article
Chemistry, Multidisciplinary
Dulini Fernando, Motilal Mathesh, Jackie Cai, Wenrong Yang
Summary: A facile polymer-directed metal-organic framework (MOF)-based nanoarchitechtonics was developed to enhance the catalytic efficiency of multi-enzyme cascade reactions through substrate channeling. The new method involved the simultaneous synthesis of MOF and co-immobilization of enzymes, resulting in a closely packed nanoarchitecture with enhanced substrate channeling. The enzyme cascade reaction system showed a 3.5-fold increase in catalytic activity compared to free enzymes.
Article
Biochemistry & Molecular Biology
Natcha Rasitanon, Kornautchaya Veenuttranon, Hnin Thandar Lwin, Kanyawee Kaewpradub, Tonghathai Phairatana, Itthipon Jeerapan
Summary: This study successfully integrated glucose oxidase (GOx) with nanomaterial-modified electrodes using egg white proteins, and demonstrated the potential of this biocompatible interface in bioelectronics, such as glucose sensors and biofuel cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Multidisciplinary
Mohamad Zandieh, Juewen Liu
Summary: The article discusses the issue of calculating catalytic turnover rates of nanozymes, emphasizing that only surface atoms should be considered as active sites. Different types of nanozymes exhibit varying catalytic activity characteristics, which may be influenced by the adsorption of reaction products.
Review
Biochemical Research Methods
Rafael T. P. da Silva, Heloise Ribeiro de Barros, Daiana M. Furlan Sandrini, Susana I. Cordoba de Torresi
Summary: Controlling biocatalytic processes in extracellular medium through metallic nanoparticles offers innovative solutions by manipulating enzyme activity and efficiency with external stimuli like light, electricity, magnetism, and temperature. Combining multiple stimuli can create a more refined framework for a multiresponsive system, opening up promising opportunities for future research in nanobiocatalysis.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Biophysics
Matias Regiart, Ana Ledo, Eliana Fernandes, German A. Messina, Christopher M. A. Brett, Mauro M. Bertotti, Rui Barbosa
Summary: Highly sensitive and selective nanostructured lactate and glucose microbiosensors were developed for simultaneous determination in rat brain. The microbiosensors, based on carbon fiber microelectrodes modified with nanoporous gold, exhibited enhanced sensitivity and electrocatalytic properties towards H2O2 detection. The selective measurements were achieved by enzyme immobilization, permselective layer, and a null sensor. The microbiosensor platform was used to determine lactate and glucose in blood serum samples, as well as in vivo measurements of basal extracellular concentrations in different rat brain structures.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Physical
Lili Xu, Jinxing Chen, Qian Ma, Daiyong Chao, Xinyang Zhu, Ling Liu, Jin Wang, Youxing Fang, Shaojun Dong
Summary: Polymer stabilizers greatly influence the structure, size, and activity of gold nanoparticles (Au NPs). Among the polymers tested, polyacrylic acid-stabilized Au NPs exhibit the highest glucose oxidase-like activity due to their small size and weak spatial resistance. However, further tests reveal limitations in selectivity and activity in physiological environments.
Article
Chemistry, Multidisciplinary
Teng Yuan, Qi Tang, Chuan Shan, Xiaohan Ye, Jin Wang, Pengyi Zhao, Lukasz Wojtas, Nicholas Hadler, Hao Chen, Xiaodong Shi
Summary: This study presents a novel method for alkyne trifunctionalization through gold catalysis, achieving consecutive reactions with high efficiency and excellent yields under mild conditions.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Review
Chemistry, Physical
I. Mazurenko, T. Adachi, B. Ezraty, M. Ilbert, K. Sowa, E. Lojou
Summary: Multicopper oxidases are a widely studied family of enzymes involved in catalyzing the oxygen reduction reaction. This paper focuses on the less studied class of copper efflux oxidases, discussing the role of the methionine-rich domain in the electron transfer process and its implications in therapeutic applications.
CURRENT OPINION IN ELECTROCHEMISTRY
(2022)
Review
Biotechnology & Applied Microbiology
Yuhan Fu, Jialin Sun, Yanhong Wang, Weinan Li
Summary: Cancer is a significant threat to human health, and traditional therapies have limitations, prompting the need for new treatment strategies. Starvation therapy, which targets the primary energy source of cancer cells, glucose, has received considerable attention. Glucose oxidase has emerged as a critical therapeutic agent in starvation therapy. However, mono therapy struggles to suppress tumor growth completely, necessitating the development of synergistic therapy approaches. Metal catalysts can serve as carriers and combine with glucose oxidase for diverse tumor treatments.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Yu Cai, Tingting Tu, Tianyu Li, Shanshan Zhang, Bin Zhang, Lu Fang, Xuesong Ye, Bo Liang
Summary: The study on the application of nanomaterials in glucose biosensors demonstrates excellent electrochemical performance, taking a step towards the development of the third-generation glucose biosensor.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Jiahui Wu, Qiaoting Yang, Qian Li, Haiyin Li, Feng Li
Summary: In this study, manganese dioxide nanosheets were used as catalysts to develop a novel electrochemical sensor for organophosphate pesticides detection, eliminating interference from H2O2 and color. The sensor showed improved performance and lower detection limits, providing a new pathway for nanozyme-based sensors and finding more applications in food samples for OP residue determination.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Physical
Zhanpo Han, Xiaoyan Zhang, Hua Yuan, Zhende Li, Guangzhen Li, Huayu Zhang, Yeqiang Tan
Summary: This study investigates a promising electrochemical sensor with GO/AuNP-modified electrodes to improve charge transport and sensitivity for glucose detection. The sensor shows a linear response and low detection limit, with good selectivity for other interfering molecules.
JOURNAL OF POWER SOURCES
(2022)
Article
Chemistry, Physical
Eimantas Ramonas, Atefeh Shafaat, Marius Dagys, Tautgirdas Ruzgas, Dalius Ratautas
Summary: This study investigated the catalytic properties of citrate-capped gold nanoparticles and gold nanoparticle-protein conjugates, showing that gold nanoparticles form stable conjugates with proteins but do not increase enzyme activity. Under certain conditions, gold nanoparticles can mimic enzymatic reactions, emphasizing the need for specific measurements to differentiate between enzyme and gold nanoparticle-catalyzed reactions.
JOURNAL OF CATALYSIS
(2021)
Article
Chemistry, Physical
I-Hsuan Li, Tien-Hao Hsieh, Zhi-Wen Chen, Hsiang-Chun Tsao, Yu-Hsuan Huang, Ching-Ping Liu
Summary: Composites or hybrids, such as SiO2-AuNPs, have been investigated for their enhanced catalytic performance and new catalytic activities. This study demonstrates that SiO2-AuNPs exhibit both peroxidase-like and oxidase-like activities, with the contribution of SiO2 mainly involved in the peroxidase-like reaction. Furthermore, the oxidase-like activity of SiO2-AuNPs can be enhanced by visible light irradiation, and electron transfer between SiO2 and AuNPs is also observed.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2023)
Review
Biochemical Research Methods
Stefan Scheiblbrandner, Florian Csarman, Roland Ludwig
Summary: Enzymatic biofuel cells use enzymes as catalysts to convert fuel and oxidant into electric energy, with wide applications in powering small electronic devices.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Gregorio Laucirica, Yamili Toum Terrones, Michael F. P. Wagner, Vanina M. Cayon, Maria Lorena Cortez, Maria Eugenia Toimil-Molares, Christina Trautmann, Waldemar Marmisolle, Omar Azzaroni
Summary: Nanofluidic channels, called nanofluidic field effect transistors (nFETs), can modulate ionic transport by using an external voltage. In this study, the actuation range of nFETs was extended by using the supporting electrolyte as a chemical effector. By changing the electrolyte nature, different ion transport regimes were set, and the rectification efficiency was controlled by the gate voltage.
Article
Nanoscience & Nanotechnology
Gonzalo E. Fenoy, Roger Hasler, Christoph Lorenz, Jacopo Movilli, Waldemar A. Marmisolle, Omar Azzaroni, Jurriaan Huskens, Peter Baeuerle, Wolfgang Knoll
Summary: Clickable organic electrochemical transistors (OECTs) enable the functionalization of electronic devices through click chemistry. This study examines various aspects of click chemistry-based interface engineering for OECTs, including investigating different channel architectures and studying different reaction conditions for the click reaction. The authors also demonstrate successful immobilization of biomolecules on OECTs for use as biosensors. The performance of these biosensors is compared to previous architectures, showing improved density of immobilized molecules and similar binding affinity and signal change.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Marjorie Montero-Jimenez, Francisco L. L. Amante, Gonzalo E. E. Fenoy, Juliana Scotto, Omar Azzaroni, Waldemar A. A. Marmisolle
Summary: This work investigates the use of PEDOT-polyamine blends as versatile organic films for conducting channels and non-denaturing platforms in OECT-based biosensors. The response of the devices to protein adsorption using two different strategies, direct electrostatic adsorption and specific recognition, is studied. The stability of protein adsorption and assembly is monitored using surface plasmon resonance and OECT, and the sensing mechanisms for the two strategies are discussed.
Review
Nanoscience & Nanotechnology
Marjorie Montero-Jimenez, Juan Lugli-Arroyo, Gonzalo E. Fenoy, Esteban Piccinini, Wolfgang Knoll, Waldemar A. Marmisolle, Omar Azzaroni
Summary: Organic electrochemical transistors (OECTs) are important devices for flexible and wearable sensors. This study presents OECTs based on PEDOT-polyamine thin films for selective monitoring of phosphate-containing compounds. The research findings show that supramolecular single phosphate-amino interaction induces higher changes in the OECT response compared to ATP-amino interactions, and the OECT response reflects the polymer/solution interface as well as events within the conducting polymer film. The study also reveals the influence of phosphate species on enzyme immobilization behavior.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Nanoscience & Nanotechnology
Eugenia Apuzzo, Maximiliano Agazzi, Santiago E. Herrera, Agustin Picco, Gaston Rizzo, Camila Chavero, Daiana Bianchi, Paola Smaldini, Maria Lorena Cortez, Waldemar A. Marmisolle, Gisel Padula, Analia Seoane, Maria Lis Alomar, Maria Paula Denofrio, Guillermo Docena, Omar Azzaroni
Summary: In this study, we optimized the robustness and safety of polyamine-multivalent molecule nanocomplexes by finely modulating their formation parameters. These nanocomplexes exhibit tremendous long-term stability and are suitable for protein-loading. Furthermore, the nanocomplexes switch-off the toxicity of the system by altering the overall charge. They can also be internalized by bone-marrow-derived macrophage cells, making them promising delivery nanoplatforms for therapies based on immune system activation.
ACS APPLIED BIO MATERIALS
(2023)
Review
Chemistry, Physical
Santiago E. Herrera, Maximiliano L. Agazzi, Eugenia Apuzzo, M. Lorena Cortez, Waldemar A. Marmisolle, Mario Tagliazucchi, Omar Azzaroni
Summary: The complexation of polyelectrolytes with other oppositely charged structures has led to the development of various functional materials with potential applications in many technological fields. Recent research has focused on the complexation of polyelectrolytes with alternative building blocks such as small charged molecules, and the similarities between these complexes and well-known polycation-polyanion complexes. This review discusses the physicochemical characteristics of these complexes and their potential as versatile functional platforms in fields such as biomedicine and advanced materials engineering.
Correction
Chemistry, Multidisciplinary
Gonzalo E. Fenoy, Roger Hasler, Felice Quartinello, Waldemar A. Marmisolle, Christoph Lorenz, Omar Azzaroni, Peter Bauerle, Wolfgang Knoll
Correction
Chemistry, Multidisciplinary
Gonzalo E. Fenoy, Roger Hasler, Felice Quartinello, Waldemar A. Marmisolle, Christoph Lorenz, Omar Azzaroni, Peter Baeuerle, Wolfgang Knoll
Article
Chemistry, Multidisciplinary
Gonzalo E. Fenoy, Roger Hasler, Felice Quartinello, Waldemar A. Marmisolle, Christoph Lorenz, Omar Azzaroni, Peter Bauerle, Wolfgang Knoll
Summary: This article presents the synthesis and characterization of clickable organic electrochemical transistors (OECTs) and demonstrates their applications in the interface of biological elements and biosensors. By clicking specific molecules onto the transistor surface, efficient binding with proteins and enzymes is achieved. The clicked OECTs maintain their stability and electronic features after the clicking reactions.
Article
Engineering, Electrical & Electronic
Gonzalo Eduardo Fenoy, Juliana Scotto, Juan A. Allegretto, Esteban Piccinini, Agustin Lorenzo Cantillo, Wolfgang Knoll, Omar Azzaroni, Waldemar Alejandro Marmisolle
Summary: This work presents the fabrication of PEDOT-PAH-based organic electrochemical transistors (OECTs) and their application in monitoring the deposition of polyelectrolyte multilayers on their surface. The electrical characteristics of the devices are optimized through different synthesis conditions, and the layer-by-layer assembly process is investigated. OECTs are proven to be capable of real-time monitoring the assembly of different polyelectrolyte layers and analyzing the changes in ionic transport. The importance of OECTs in sensing charged macromolecules is demonstrated through comparison with other sensing platforms.
ACS APPLIED ELECTRONIC MATERIALS
(2022)
Article
Engineering, Electrical & Electronic
Juliana Scotto, Agustin L. Cantillo, Esteban Piccinini, Gonzalo E. Fenoy, Juan A. Allegretto, Jose M. Piccinini, Waldemar A. Marmisolle, Omar Azzaroni
Summary: The study demonstrates that gFET technology can be used for real-time monitoring of polyelectrolyte adsorption, compared and validated against surface plasmon resonance, showing the superiority of using a silver/silver chloride coplanar gate electrode.
ACS APPLIED ELECTRONIC MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Yamili Toum Terrones, Gregorio Laucirica, Vanina M. Cayon, Gonzalo E. Fenoy, M. Lorena Cortez, Maria Eugenia Toimil-Molares, Christina Trautmann, Waldemar A. Mamisolle, Omar Azzaroni
Summary: Acetylcholinesterase-modified nanochannels are reliable and reproducible nanofluidic sensors for highly sensitive detection of acetylcholine. The surface charge of the nanochannel is adjusted/reconfigured by weak polyelectrolytes as chemical amplifiers in the presence of local pH changes induced by the enzymatic reaction, allowing the transduction of acetylcholine into measurable ionic signals with a low limit of detection.
CHEMICAL COMMUNICATIONS
(2022)
