Article
Biophysics
Xiaomei Yan, Jing Tang, Su Ma, David Tanner, Roland Ludwig, Jens Ulstrup, Xinxin Xiao
Summary: A novel three-dimensional graphene-based carbon electrode has been successfully utilized for immobilizing Myriococcum thermophilum cellobiose dehydrogenase. Detailed structural and electrochemical characterizations reveal favorable performance in glucose oxidation reactions. The constructed entirely DET-based enzymatic biofuel cell demonstrates excellent power output and reliability.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemistry & Molecular Biology
Lorgio Victor Bautista Samaniego, Paula Miwa Rabelo Higasi, Caio Cesar de Mello Capetti, Anelyse Abreu Cortez, Sebastiao Pratavieira, Vanessa de Oliveira Arnoldi Pellegrini, Andrei Nicoli Gebieluca Dabul, Fernando Segato, Igor Polikarpov
Summary: This work presents the biochemical characteristics and application of TthCDHIIa from Thermothelomyces thermophilus as an antimicrobial and antibiofilm agent. TthCDHIIa is thermostable and can oxidize various mono and oligosaccharide substrates, producing H2O2. It exhibits catalytic characteristics consistent with an Ascomycota class II CDH. TthCDHIIa can inhibit the viability and biofilm growth of S. aureus, and can also enhance the susceptibility of biofilms to gentamicin and tetracycline through oxidative degradation of the biofilm matrix using H2O2.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Multidisciplinary Sciences
Jiraporn Jirakkakul, Nuchnudda Wichienchote, Somsak Likhitrattanapisal, Supawadee Ingsriswang, Thippawan Yoocha, Sithichoke Tangphatsornruang, Rudsamee Wasuwan, Supapon Cheevadhanarak, Morakot Tanticharoen, Alongkorn Amnuaykanjanasin
Summary: The putative ferricrocin synthetase gene ferS in Beauveria bassiana BCC 2660 encodes a multimodular nonribosomal siderophore synthetase clustered with Fusarium graminearum synthetase. Disruption of ferS abolished biosynthesis of ferricrocin, resulting in increased growth and germination under iron depletion and higher insect mortality in the early phase of infection compared to wild type, possibly due to up-regulation of antioxidant-related genes and pathways in ergosterol biosynthesis and TCA cycle.
SCIENTIFIC REPORTS
(2021)
Review
Agricultural Engineering
P. Sai Preethi, N. M. Hariharan, Sundaram Vickram, M. Rameshpathy, S. Manikandan, R. Subbaiya, N. Karmegam, Vivek Yadav, Balasubramani Ravindran, S. W. Chang, Mukesh Kumar Awasthi
Summary: The use of enzyme biotechnology for the bioremediation of emerging pollutants in wastewater offers a cost-effective and low-energy approach. However, this technology is still not fully developed for commercial use. Oxidoreductases, as dominant biocatalysts, show promise for wastewater treatments. Therefore, understanding their global market and biotransformation efficacy is essential for establishing these bio-enzymes in commercial scale. Combining the biocatalytic strategy with existing treatment technology can achieve effective removal of emerging pollutants from wastewater. This review provides insights into the treatment of toxicological xenobiotics from various industries and the potential of peroxidases, nitroreductase, and cellobiose dehydrogenase in their bio-based treatment. Additionally, it comprehensively covers the environmental relevance of wastewater pollution and the challenges faced in terms of biocatalytic remediation for future prospects.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Justyna Sulej, Monika Osinska-Jaroszuk, Magdalena Jaszek, Anna Olszewska, Anna Belcarz, Wiktoria Piatek-Golda
Summary: The study aimed to immobilize cellobiose dehydrogenase (CDH) on chitosan beads and characterize the properties of immobilized CDHs from different fungal sources. The immobilized CDHs were characterized using FTIR spectra and SEM microstructure. The most effective method of immobilization was the covalent bonding of enzyme molecules using glutaraldehyde, showing efficiencies ranging from 28% to 99%. Promising results were obtained in terms of antioxidant, antimicrobial, and cytotoxic properties compared to free CDH. Chitosan appears to be a valuable material for the development of immobilization systems for biomedical applications or food packaging, while preserving the unique properties of CDH.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agricultural Engineering
Man Zhou, Lizhi Tao, Peter Russell, R. David Britt, Takao Kasuga, Xin Lu, Zhiliang Fan
Summary: The engineered Neurospora crassa strain HL10 can convert cellulose into cellobionic acid without the need for additional enzymes or redox mediators. Lignin and lignin degradation products can serve as redox mediators and enhance cellobionic acid production in the CDH-laccase conversion system.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Microbiology
Justyna Sulej, Magdalena Jaszek, Monika Osinska-Jaroszuk, Anna Matuszewska, Renata Bancerz, Monika Janczarek
Summary: This study demonstrates that natural polysaccharides can enhance the stability of CDH, making them promising biotechnological tools for practical applications in various industries.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Melisa del Barrio, Emiliano Martinez-Perinan, Cristina Gutierrez-Sanchez, Eva Mateo-Marti, Marcos Pita, Antonio L. De Lacey, Felix Pariente, Encarnacion Lorenzo
Summary: The determination of cholesterol is important for heart disease detection and as a fuel for enzymatic fuel cells. A cholesterol biosensor and peroxide-free biofuel cell was developed using electrocatalytic oxidation of NADH generated during cholesterol dehydrogenase (ChDH) reaction. This alternative method avoids the use of H2O2 biosensing strategies.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Multidisciplinary Sciences
Taku Uchiyama, Takayuki Uchihashi, Takuya Ishida, Akihiko Nakamura, Josh V. Vermaas, Michael F. Crowley, Masahiro Samejima, Gregg T. Beckham, Kiyohiko Igarashi
Summary: Efficient depolymerization of crystalline cellulose requires the cooperative action of multiple cellulolytic enzymes. This study demonstrates that the synergistic activity between cellobiohy-drolases and a lytic polysaccharide monooxygenase can enhance the activity and performance of the enzymes by producing chain breaks and amorphizing the crystalline surface of cellulose.
Article
Chemistry, Physical
Alfons K. G. Felice, Christian Schuster, Alan Kadek, Frantisek Filandr, Christophe V. F. P. Laurent, Stefan Scheiblbrandner, Lorenz Schwaiger, Franziska Schachinger, Daniel Kracher, Christoph Sygmund, Petr Man, Petr Halada, Chris Oostenbrink, Roland Ludwig
Summary: This study elucidates the role of the cytochrome (CYT) domain of cellobiose dehydrogenase (CDH) in two electron transfer steps, revealing key factors and protein conformational changes in interdomain electron transfer.
Review
Chemistry, Multidisciplinary
Phan Gia Le, Moon Il Kim
Summary: The evolution of biofuel cells can be categorized into microbial biofuel cells, enzymatic biofuel cells, and nanozyme-based biofuel cells. MBFCs can produce electricity from waste with low power output, while EBFCs are vulnerable under diverse environmental conditions. NBFCs using nanozymes have advantages such as high stability and low synthesis cost, making them attractive for developing wearable and implantable devices for electricity generation.
Review
Chemistry, Physical
Abhilasha Mishra, Rinkesh Bhatt, Jaya Bajpai, A. K. Bajpai
Summary: Biofuel cells (BFCs) utilize metabolic reactions in microorganisms to transform the chemical energy of organic matter into electrical energy, but face challenges such as short life-time and low-power density. The use of nano materials is a key strategy to enhance the efficiency of BFCs, improving electron transfer and current output through various nanostructures. Different metallic nanomaterials, inorganic nanomaterials, conducting polymer-based nanomaterials, and carbon-based nano materials are utilized to construct electrodes and membranes for better performance of BFCs.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Article
Biochemistry & Molecular Biology
Junhua Yang, Peng Xu, Liangkun Long, Shaojun Ding
Summary: Lactobionic acid (LBA) has promising applications in various fields and can be efficiently produced using a new cellobiose dehydrogenase. The immobilized AfCDH/LAC system demonstrates high efficiency and reusability in LBA production, making it a viable option for industrial implementation.
PROCESS BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Parvin Rafighi, Eva Nordberg Karlsson, Kazi Zubaida Gulshan Ara, Galina Pankratova, Paolo Bollella, Clemens K. Peterbauer, Lo Gorton
Summary: A novel membraneless enzymatic fuel cell using beta-glucan as fuel was developed. The fuel was produced by enzymatic hydrolysis of beta-glucan, which was then oxidized by specific enzymes to generate a current response. The fuel cell showed high efficiency and excellent stability.
BIOELECTROCHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Zhanfeng Wang, Shishi Feng, Carme Rovira, Binju Wang
Summary: Long-range electron transfer between cellobiose dehydrogenase (CDH) and lytic polysaccharide monooxygenases (LPMOs) is mediated by the heme propionates of CDH and solvent waters. Oxygen binding to the copper center of LPMO is coupled with the long-range interprotein electron transfer, leading directly to the formation of LPMO-Cu-II-O-2(-) species. This newly uncovered electron transfer mechanism rationalizes experimental observations and may have implications for catalysis in LPMOs and other metalloenzymes with O-2- or CO-binding-enhanced long-range electron transfer processes.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Christophe V. F. P. Laurent, Peicheng Sun, Stefan Scheiblbrandner, Florian Csarman, Pietro Cannazza, Matthias Frommhagen, Willem J. H. van Berkel, Chris Oostenbrink, Mirjam A. Kabel, Roland Ludwig
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Biotechnology & Applied Microbiology
Lena Wohlschlager, Florian Csarman, Hucheng Chang, Elisabeth Fitz, Bernhard Seiboth, Roland Ludwig
Summary: Heterologous production of PcCDHTr is faster and has a higher yield than secretion by P. chrysosporium. The uniform glycoforms of PcCDHTr are useful for investigating electron transfer characteristics in biosensors and biofuel cells. The catalytic and electrochemical properties of PcCDHTr are similar to PcCDH, making T. reesei a good expression host for engineered PcCDH in biosensors and biofuel cells.
MICROBIAL CELL FACTORIES
(2021)
Article
Biotechnology & Applied Microbiology
Lena Wohlschlager, Florian Csarman, Matea Zrilic, Bernhard Seiboth, Roland Ludwig
Summary: In this study, two efficient processes for the heterologous production of GLOX from Phanerochaete chrysosporium were reported and compared using Pichia pastoris and Trichoderma reesei as expression hosts. The enzymes produced in different hosts showed similar substrate affinities and turnover numbers, with GLOX from both hosts being suitable for further mechanistic characterization and application studies. Additionally, GLOX expressed in Pichia pastoris exhibited slightly higher specific activities due to higher copper loading compared to the enzyme from Trichoderma reesei.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Hucheng Chang, Lena Wohlschlager, Florian Csarman, Adrian Ruff, Wolfgang Schuhmann, Stefan Scheiblbrandner, Roland Ludwig
Summary: This study highlights the importance of continuous and accurate measurement of key products in the enzymatic hydrolysis process for biofuel production. By utilizing modified polymers and enzymes, specific biosensors were developed to detect cellobiose and glucose in the presence of oxygen, enabling a more precise monitoring of the hydrolysis process. The results demonstrate the reliability of the developed biosensors for industrial applications.
ANALYTICAL CHEMISTRY
(2021)
Article
Materials Science, Paper & Wood
Florian Csarman, Claudia Gusenbauer, Lena Wohlschlager, Gijs van Erven, Mirjam A. Kabel, Johannes Konnerth, Antje Potthast, Roland Ludwig
Summary: This study investigates the binding of cellulolytic enzymes onto biopolymers. Results show that the removal of N-glycans does not significantly affect the activity or binding tendency, but a slightly higher tendency for non-productive binding is observed on surfaces modified with different lignin fractions.
Article
Biotechnology & Applied Microbiology
Florian Csarman, Tobias Obermann, Mihael Colar Zanjko, Petr Man, Petr Halada, Bernhard Seiboth, Roland Ludwig
Summary: This study investigates the catalytic properties of laccases secreted by Fomitopsis pinicola FP58527 SS1 in brown rot fungi. Two laccases from the secretome, FpLcc1 and FpLcc2, were found to have similar low pH-optima for substrates such as ABTS, 2,6-DMP, and guaiacol. FpLcc2 showed activation by acetic acid, with a more pronounced effect at pH 5.0 compared to pH 3.0.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lena Wohlschlager, Daniel Kracher, Stefan Scheiblbrandner, Florian Csarman, Roland Ludwig
Summary: GLOX in white-rot secretomes acts in concert with peroxidases to degrade lignin. Its activity is influenced by oxygen concentration and activator concentration.
BIOELECTROCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Juan Antonio Mendez-Liter, Ivan Ayuso-Fernandez, Florian Csarman, Laura Isabel de Eugenio, Noa Miguez, Francisco J. Plou, Alicia Prieto, Roland Ludwig, Maria Jesus Martinez
Summary: The first lytic polysaccharide monooxygenase (LPMO) from the ascomycete Talaromyces amestolkiae has been successfully expressed and characterized. Analyses showed C1/C4 oxidation regioselectivity and confirmed the enzyme's thermostability, with implications for saccharification applications in cellulosic and lignocellulosic materials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Neus Gacias Amengual, Florian Csarman, Lena Wohlschlager, Roland Ludwig
Summary: Fungi efficiently decompose biomass using enzymatic and non-enzymatic strategies, and they are a valuable source of versatile biocatalysts. This study compared the characteristics of GH45 endoglucanases from the brown rot Fomitopsis pinicola and the white rot Phanerochaete chrysosporium, and found no significant differences between them.
ENZYME AND MICROBIAL TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Neus Gacias-Amengual, Lena Wohlschlager, Florian Csarman, Roland Ludwig
Summary: In this study, lignocellulosic enzymes from the white rot fungus Phanerochaete chrysosporium were localized on model substrates and poplar wood using confocal laser scanning microscopy. Two detection approaches were investigated, revealing the innermost secondary cell wall layer as the preferential attack point for cellulose-degrading enzymes. The methods presented are suitable for visualizing enzymes during catalytic biomass degradation and can be further utilized for interaction studies in biorefineries.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Hucheng Chang, Neus Gacias Amengual, Alexander Botz, Lorenz Schwaiger, Daniel Kracher, Stefan Scheiblbrandner, Florian Csarman, Roland Ludwig
Summary: This study demonstrates the activity of Lytic polysaccharide monooxygenase (LPMO) on wood cell walls and suggests that even low levels of hydrogen peroxide can support the enzymatic degradation of wood biomass. LPMO plays a synergistic role in fungal extracellular catabolism and industrial biomass degradation.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Bettina Motycka, Florian Csarman, Rupert Tscheliessnig, Michal Hammel, Roland Ludwig
Summary: Small angle X-ray scattering (SAXS) was used to study the mobility of the cytochrome and dehydrogenase domains of CDH from Myriococcum thermophilum. The results showed that the mobility of the cytochrome domain increases with higher pH, indicating alterations of domain mobility. On the other hand, the presence of calcium ions decreases the mobility of the cytochrome domain.
Article
Biochemistry & Molecular Biology
Bettina Motycka, Florian Csarman, Melanie Rupp, Karoline Schnabel, Gabor Nagy, Kwankao Karnpakdee, Stefan Scheiblbrandner, Rupert Tscheliessnig, Chris Oostenbrink, Michal Hammel, Roland Ludwig
Summary: This study investigates the role of two crucial amino acids in cellobiose dehydrogenase (CDH) in the interaction between the dehydrogenase and cytochrome domains, as well as in the interdomain electron transfer. The results suggest that these two residues play an essential role in shaping the domain interaction and facilitating electron transfer.
Article
Chemistry, Applied
Martin Nagl, Oskar Haske-Cornelius, Wolfgang Bauer, Florian Csarman, Roland Ludwig, Gibson S. Nyanhongo, Georg M. Guebitz
Summary: This study investigated the synergistic effects of enzymes in pulp refining and their relationship with refining performance. The results showed that enzymes with a narrow particle size distribution and a high reduction in molecular weight were beneficial for refining cellulose fibers. Additionally, the combination of endoglucanases with beta-glucosidases or xylanases improved the refining degree and tensile strength of the paper.
CARBOHYDRATE POLYMER TECHNOLOGIES AND APPLICATIONS
(2022)
Review
Biochemical Research Methods
Pei-Ru Chen, Peng-Fei Xia
Summary: Carbon dioxide plays a crucial role in mitigating global climate change and supporting life on Earth. Synthetic biology and metabolic engineering offer a promising approach to enhance CO2 fixation by combining the advantages of autotrophic and heterotrophic organisms. This article reviews the current progress in constructing synthetic CO2 fixation pathways, discusses the underlying design principles and challenges, and provides insights into the future of carbon recycling through engineered synthetic carbon fixation pathways.
CURRENT OPINION IN BIOTECHNOLOGY
(2024)
