Many Pathways in Laboratory Evolution Can Lead to Improved Enzymes: How to Escape from Local Minima

When Second Best Is Good Enough: Another Probabilistic Look at Saturation Mutagenesis

(2011) Yuval Nov   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Enhancing the Thermal Robustness of an Enzyme by Directed Evolution: Least Favorable Starting Points and Inferior Mutants Can Map Superior Evolutionary Pathways

(2011) Yosephine Gumulya et al.   CHEMBIOCHEM

Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution

(2011) Yusuke Miyauchi et al.   CHEMICAL COMMUNICATIONS

Revisiting the Lipase from Pseudomonas aeruginosa: Directed Evolution of Substrate Acceptance and Enantioselectivity Using Iterative Saturation Mutagenesis

(2011) Shreenath Prasad et al.   CHEMPHYSCHEM

Engineering GST M2-2 for High Activity with Indene 1,2-Oxide and Indication of an H-Site Residue Sustaining Catalytic Promiscuity

(2011) Malena A. Norrgård et al.   JOURNAL OF MOLECULAR BIOLOGY

Combinatorial reshaping of the Candida antarctica lipase A substrate pocket for enantioselectivity using an extremely condensed library

(2011) A. G. Sandstrom et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Negative Epistasis Between Beneficial Mutations in an Evolving Bacterial Population

(2011) A. I. Khan et al.   SCIENCE

Laboratory Evolution of Stereoselective Enzymes: A Prolific Source of Catalysts for Asymmetric Reactions

(2010) Manfred T. Reetz   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Status of protein engineering for biocatalysts: how to design an industrially useful biocatalyst

(2010) Andreas S Bommarius et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Stabilization of Escherichia coli uridine phosphorylase by evolution and immobilization

(2010) Daniel F. Visser et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Manipulating the Stereoselectivity of Limonene Epoxide Hydrolase by Directed Evolution Based on Iterative Saturation Mutagenesis

(2010) Huabao Zheng et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Directed Evolution of an Enantioselective Lipase with Broad Substrate Scope for Hydrolysis of α-Substituted Esters

(2010) Karin Engström et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Iterative Saturation Mutagenesis Accelerates Laboratory Evolution of Enzyme Stereoselectivity: Rigorous Comparison with Traditional Methods

(2010) Manfred T. Reetz et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Directed Evolution of an Enantioselective Enoate-Reductase: Testing the Utility of Iterative Saturation Mutagenesis

(2009) Despina J. Bougioukou et al.   ADVANCED SYNTHESIS & CATALYSIS

Structure-Guided Directed Evolution of Alkenyl and Arylmalonate Decarboxylases

(2009) Krzysztof Okrasa et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Identification of Emerging Quasi-Species in Directed Enzyme Evolution

(2009) Sanela Kurtovic et al.   BIOCHEMISTRY

Advances in generating functional diversity for directed protein evolution

(2009) Amol V Shivange et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Engineering Monolignol 4-O-Methyltransferases to Modulate Lignin Biosynthesis

(2009) Mohammad-Wadud Bhuiya et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure-Based and Random Mutagenesis Approaches Increase the Organophosphate-Degrading Activity of a Phosphotriesterase Homologue from Deinococcus radiodurans

(2009) Renda Hawwa et al.   JOURNAL OF MOLECULAR BIOLOGY

Directed Evolution of an Enantioselective Epoxide Hydrolase: Uncovering the Source of Enantioselectivity at Each Evolutionary Stage

(2009) Manfred T. Reetz et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Finding better protein engineering strategies

(2009) Romas J Kazlauskas et al.   Nature Chemical Biology

Directed evolution drives the next generation of biocatalysts

(2009) Nicholas J Turner   Nature Chemical Biology

Exploring protein fitness landscapes by directed evolution

(2009) Philip A. Romero et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Creating lactose phosphorylase enzymes by directed evolution of cellobiose phosphorylase

(2009) M. R.M. De Groeve et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Enzyme engineering for enantioselectivity: from trial-and-error to rational design?

(2009) Linda G. Otten et al.   TRENDS IN BIOTECHNOLOGY

Protein Design by Directed Evolution

(2008) Christian Jäckel et al.   Annual Review of Biophysics

Constructing and Analyzing the Fitness Landscape of an Experimental Evolutionary Process

(2008) Manfred T. Reetz et al.   CHEMBIOCHEM

A Novel Genetic Selection System for Improved Enantioselectivity ofBacillus subtilis Lipase A

(2008) Ykelien L. Boersma et al.   CHEMBIOCHEM

Addressing the Numbers Problem in Directed Evolution

(2008) Manfred T. Reetz et al.   CHEMBIOCHEM

A genetic selection system for evolving enantioselectivity of enzymes

(2008) Manfred T. Reetz et al.   CHEMICAL COMMUNICATIONS

Greatly reduced amino acid alphabets in directed evolution: making the right choice for saturation mutagenesis at homologous enzyme positions

(2008) Manfred T. Reetz et al.   CHEMICAL COMMUNICATIONS

Advances in laboratory evolution of enzymes

(2008) Shimon Bershtein et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Shedding light on the efficacy of laboratory evolution based on iterative saturation mutagenesis

(2008) Manfred T. Reetz et al.   Molecular BioSystems

Enzyme optimization: moving from blind evolution to statistical exploration of sequence–function space

(2008) Richard J. Fox et al.   TRENDS IN BIOTECHNOLOGY