Enhancing the Thermal Robustness of an Enzyme by Directed Evolution: Least Favorable Starting Points and Inferior Mutants Can Map Superior Evolutionary Pathways

Engineering GST M2-2 for High Activity with Indene 1,2-Oxide and Indication of an H-Site Residue Sustaining Catalytic Promiscuity

(2011) Malena A. Norrgård et al.   JOURNAL OF MOLECULAR BIOLOGY

Regio- and stereoselectivity of P450-catalysed hydroxylation of steroids controlled by laboratory evolution

(2011) Sabrina Kille et al.   Nature Chemistry

Laboratory Evolution of Stereoselective Enzymes: A Prolific Source of Catalysts for Asymmetric Reactions

(2010) Manfred T. Reetz   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Inverting the enantioselectivity of P450pyr monooxygenase by directed evolution

(2010) Weng Lin Tang et al.   CHEMICAL COMMUNICATIONS

Status of protein engineering for biocatalysts: how to design an industrially useful biocatalyst

(2010) Andreas S Bommarius et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

The quest for molecular quasi-species in ligand-activity space and its application to directed enzyme evolution

(2010) Bengt Mannervik et al.   FEBS LETTERS

Thermostabilization of Bacillus circulans xylanase: Computational optimization of unstable residues based on thermal fluctuation analysis

(2010) Jeong Chan Joo et al.   JOURNAL OF BIOTECHNOLOGY

A Novel Quasi-Species of Glutathione Transferase with High Activity towards Naturally Occurring Isothiocyanates Evolves from Promiscuous Low-Activity Variants

(2010) Arna Runarsdottir et al.   JOURNAL OF MOLECULAR BIOLOGY

Stabilization of Escherichia coli uridine phosphorylase by evolution and immobilization

(2010) Daniel F. Visser et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Directed Evolution of an Enantioselective Lipase with Broad Substrate Scope for Hydrolysis of α-Substituted Esters

(2010) Karin Engström et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Iterative Saturation Mutagenesis Accelerates Laboratory Evolution of Enzyme Stereoselectivity: Rigorous Comparison with Traditional Methods

(2010) Manfred T. Reetz et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast

(2010) Eva García-Ruiz et al.   Microbial Cell Factories

Thermostabilization of an esterase by alignment-guided focussed directed evolution

(2010) Helge Jochens et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Directed Evolution of an Enantioselective Enoate-Reductase: Testing the Utility of Iterative Saturation Mutagenesis

(2009) Despina J. Bougioukou et al.   ADVANCED SYNTHESIS & CATALYSIS

Structure-Guided Directed Evolution of Alkenyl and Arylmalonate Decarboxylases

(2009) Krzysztof Okrasa et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Identification of Emerging Quasi-Species in Directed Enzyme Evolution

(2009) Sanela Kurtovic et al.   BIOCHEMISTRY

Advances in generating functional diversity for directed protein evolution

(2009) Amol V Shivange et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Engineering Monolignol 4-O-Methyltransferases to Modulate Lignin Biosynthesis

(2009) Mohammad-Wadud Bhuiya et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Optimizing Protein Stability In Vivo

(2009) Linda Foit et al.   MOLECULAR CELL

Directed evolution drives the next generation of biocatalysts

(2009) Nicholas J Turner   Nature Chemical Biology

Exploring protein fitness landscapes by directed evolution

(2009) Philip A. Romero et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

In the light of directed evolution: Pathways of adaptive protein evolution

(2009) J. D. Bloom et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Creating lactose phosphorylase enzymes by directed evolution of cellobiose phosphorylase

(2009) M. R.M. De Groeve et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Thermally denatured state determines refolding in lipase: Mutational analysis

(2009) Shoeb Ahmad et al.   PROTEIN SCIENCE

Enzyme engineering for enantioselectivity: from trial-and-error to rational design?

(2009) Linda G. Otten et al.   TRENDS IN BIOTECHNOLOGY

Protein Design by Directed Evolution

(2008) Christian Jäckel et al.   Annual Review of Biophysics

Enhancement of thermostability of fungal deglycating enzymes by directed evolution

(2008) Kozo Hirokawa et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Enzymatic Analysis of a Thermostabilized Mutant of anEscherichia coliHygromycin B Phosphotransferase

(2008) Akira NAKAMURA et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Enzyme assays

(2008) Jean-Louis Reymond et al.   CHEMICAL COMMUNICATIONS

A Cell-Based Adrenaline Assay for Automated High-Throughput Activity Screening of Epoxide Hydrolases

(2008) Daniel Kahakeaw et al.   Chemistry-An Asian Journal

Advances in laboratory evolution of enzymes

(2008) Shimon Bershtein et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Shedding light on the efficacy of laboratory evolution based on iterative saturation mutagenesis

(2008) Manfred T. Reetz et al.   Molecular BioSystems