Revisiting the Lipase from Pseudomonas aeruginosa: Directed Evolution of Substrate Acceptance and Enantioselectivity Using Iterative Saturation Mutagenesis

Laboratory Evolution of Stereoselective Enzymes: A Prolific Source of Catalysts for Asymmetric Reactions

(2010) Manfred T. Reetz   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Directed Evolution of an Enantioselective Lipase with Broad Substrate Scope for Hydrolysis of α-Substituted Esters

(2010) Karin Engström et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Iterative Saturation Mutagenesis Accelerates Laboratory Evolution of Enzyme Stereoselectivity: Rigorous Comparison with Traditional Methods

(2010) Manfred T. Reetz et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Rational assignment of key motifs for function guides in silico enzyme identification

(2010) Matthias Höhne et al.   Nature Chemical Biology

Induced allostery in the directed evolution of an enantioselective Baeyer-Villiger monooxygenase

(2010) S. Wu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Directed Evolution of an Enantioselective Enoate-Reductase: Testing the Utility of Iterative Saturation Mutagenesis

(2009) Despina J. Bougioukou et al.   ADVANCED SYNTHESIS & CATALYSIS

Advances in generating functional diversity for directed protein evolution

(2009) Amol V Shivange et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Laboratory Evolution of Robust and Enantioselective Baeyer−Villiger Monooxygenases for Asymmetric Catalysis

(2009) Manfred T. Reetz et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Finding better protein engineering strategies

(2009) Romas J Kazlauskas et al.   Nature Chemical Biology

Directed evolution drives the next generation of biocatalysts

(2009) Nicholas J Turner   Nature Chemical Biology

Exploring protein fitness landscapes by directed evolution

(2009) Philip A. Romero et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Enzyme engineering for enantioselectivity: from trial-and-error to rational design?

(2009) Linda G. Otten et al.   TRENDS IN BIOTECHNOLOGY

Protein Design by Directed Evolution

(2008) Christian Jäckel et al.   Annual Review of Biophysics

Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates

(2008) Joaquin Sanchis et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Addressing the Numbers Problem in Directed Evolution

(2008) Manfred T. Reetz et al.   CHEMBIOCHEM

A genetic selection system for evolving enantioselectivity of enzymes

(2008) Manfred T. Reetz et al.   CHEMICAL COMMUNICATIONS

Enzyme assays

(2008) Jean-Louis Reymond et al.   CHEMICAL COMMUNICATIONS

Advances in laboratory evolution of enzymes

(2008) Shimon Bershtein et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Enzyme optimization: moving from blind evolution to statistical exploration of sequence–function space

(2008) Richard J. Fox et al.   TRENDS IN BIOTECHNOLOGY