Article
Biochemistry & Molecular Biology
Keren Friedman, Ofri Karmon, Uri Fridman, Yair Goldberg, Ophry Pines, Shay Ben-Aroya
Summary: Previous studies have shown that dysfunctional yeast proteasomes accumulate in the insoluble protein deposit (IPOD) and are prone to proteasome ubiquitination. However, our study reveals that only soluble proteasomes are targeted for autophagy, challenging the previous belief that sequestered proteasomes in IPOD are the substrates for proteaphagy. Moreover, the IPOD serves as an alternative pathway for the removal of inactive proteasomes that are not cleared by proteaphagy. This suggests that the relocalization of proteasomes to soluble aggregates is a crucial step in proteasome recycling through autophagy.
Article
Biochemistry & Molecular Biology
Jun-Hao Wen, Xiang-Hong He, Ze-Sen Feng, Dong-Yi Li, Ji-Xin Tang, Hua-Feng Liu
Summary: The accumulation of protein aggregates is a characteristic feature of neurodegenerative diseases. Protein homeostasis imbalance caused by proteotoxic stress or mutant proteins leads to protein aggregation, interfering with cellular processes and promoting the progression of age-related neurodegenerative diseases. Cells have evolved mechanisms to rescue or eliminate protein aggregates, and potential therapeutic strategies targeting protein aggregates are being explored.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mei Pu, Yusi Tai, Luyang Yuan, Yu Zhang, Huijie Guo, Zongbing Hao, Jing Chen, Xinming Qi, Guanghui Wang, Zhouteng Tao, Jin Ren
Summary: Poly-GA, a dipeptide repeat protein derived from C9orf72, plays a key role in the pathogenesis of C9orf72-related ALS/FTD by affecting the interaction between proteasomes and autophagy. Rapamycin treatment can effectively alleviate degenerative symptoms, reduce neuronal injury and neuroinflammation in mice expressing 150 repeats poly-GA.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Yaping Liu, Runrong Ding, Ze Xu, Yuan Xue, Dongdong Zhang, Yujing Zhang, Wenjie Li, Xing Li
Summary: Alzheimer's disease is characterized by the formation of senile plaques and neurofibrillary tangles in the brain, but these pathologic biomarkers can only be detected after a sustained disease progression. The challenge in AD treatment is the lack of effective biomarkers for early diagnosis. The protein quality control system plays a crucial role in AD, as the accumulation of abnormal proteins is closely related to its dysfunction. Therefore, focusing on the protein quality control system can provide novel therapeutic strategies for preventing AD development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Neurosciences
Margreet B. Koopman, Luca Ferrari, Stefan G. D. Ruediger
Summary: Protein aggregation is a characteristic feature of neurodegenerative diseases. The protein quality control system usually prevents misfolding and accumulation of proteins, but this control mechanism is somehow evaded in diseases. This review discusses the role of protein quality control in protein aggregation and neurodegeneration, with a particular focus on Tau protein in Alzheimer's disease and other tauopathies. Recent advances in amyloid fibril structures and the process of fibril formation through phase separation are also explored, providing new insights into the role of protein quality control in protein aggregation diseases.
TRENDS IN NEUROSCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Yanhui Zhou, Hakim Manghwar, Weiming Hu, Fen Liu
Summary: Autophagy is a key pathway for nutrient recycling in eukaryotes, influenced by various factors such as hormones, second messengers, post-transcriptional regulation, and protein post-translational modification. It is activated under stress conditions to help cells survive, and the degradation mechanism of autophagy-related proteins in different organisms has attracted attention.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Plant Sciences
Marion Clavel, Yasin Dagdas
Summary: Plants have evolved multitiered quality control mechanisms to maintain the integrity of organelles under stress. Our understanding of plant organelle quality control mechanisms, especially the crosstalk between different pathways, is still incomplete. Recent advances focus on targeted protein degradation pathways and propose tools for discovering missing components in organelle quality control.
CURRENT OPINION IN PLANT BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Amanda B. Abildgaard, Vasileios Voutsinos, Soren D. Petersen, Fia B. Larsen, Caroline Kampmeyer, Kristoffer E. Johansson, Amelie Stein, Tommer Ravid, Claes Andreasson, Michael K. Jensen, Kresten Lindorff-Larsen, Rasmus Hartmann-Petersen
Summary: Protein quality control (PQC) degrons are short protein segments that target misfolded proteins for proteasomal degradation, and chaperone-binding regions may function as PQC degrons. A canonical Hsp70-binding motif, the APPY peptide, functions as a dose-dependent PQC degron in yeast and human cells. The number of exposed Hsp70-binding sites in the yeast proteome correlates with reduced protein abundance and half-life.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Review
Cell Biology
Wenjun Chen, Tianyun Shen, Lijun Wang, Kefeng Lu
Summary: This article summarizes the mechanisms of action of receptors in selective protein aggregate autophagy and recent research progress, with a particular focus on how oligomerization of receptors affects pathway determinants and promotes phase separation.
Review
Cell Biology
Erik McShane, Matthias Selbach
Summary: Cellular protein degradation is an active and highly regulated process that has diverse roles in biological functions, including homeostasis, regulation, and quality control.
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Yunting Fu, Xifeng Li, Baofang Fan, Cheng Zhu, Zhixiang Chen
Summary: Maintaining functional protein homeostasis in chloroplasts is crucial for plant fitness and survival. Research has uncovered various mechanisms involved in chloroplast protein quality control and turnover, such as endosymbiotically-derived proteases, ubiquitin-dependent turnover, chloroplast-associated degradation, chloroplast unfolded protein response, and vesicle-mediated degradation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jowita Nowakowska-Golacka, Justyna Czapiewska, Hanna Sominka, Natalia Sowa-Rogozinska, Monika Slominska-Wojewodzka
Summary: EDEM1 is an important regulatory factor involved in APP metabolism, as it recognizes misfolded APP proteins and directs them to cytosol for degradation, leading to decreased secretion of Aβ.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Carolyn Allain Breckel, Mark Hochstrasser
Summary: The proper folding of proteins is vital for their diverse functions, and misfolded proteins can potentially harm cells by forming aggregates. Protein quality control pathways are responsible for repairing or degrading abnormal proteins, with the ubiquitin-proteasome system being commonly employed.
Review
Biochemistry & Molecular Biology
Nikola Winter, Maria Novatchkova, Andreas Bachmair
Summary: The first amino acid of a protein plays a significant role in its metabolic stability, with N-degrons mediating turnover. Despite only a few proteases known to contribute to the generation of N-degrons, there is potential for further discovery in many processing paths.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Lion Borgert, Swadha Mishra, Fabian den Brave
Summary: The nucleus plays an emerging role in cellular quality control by degrading or sequestering aberrant proteins, thus protecting the cellular environment.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Cell Biology
Swastika Sanyal, Lucia Molnarova, Judita Richterova, Barbora Huraiova, Zsigmond Benko, Silvia Polakova, Ingrid Cipakova, Andrea Sevcovicova, Katarina Gaplovska-Kysela, Karl Mechtler, Lubos Cipak, Juraj Gregan
JOURNAL OF CELL SCIENCE
(2018)
News Item
Cell Biology
Katarina Gaplovska-Kysela, Andrea Sevcovicova
Article
Biochemistry & Molecular Biology
Valerie Le Fourn, Sujin Park, Insook Jang, Katarina Gaplovska-Kysela, Bruno Guhl, Yangsin Lee, Jin Won Cho, Christian Zuber, Juergen Roth
CELLULAR AND MOLECULAR LIFE SCIENCES
(2013)
Article
Cell Biology
Vendula Stradalova, Katarina Gaplovska-Kysela, Pavel Hozak
HISTOCHEMISTRY AND CELL BIOLOGY
(2008)
Review
Biochemistry & Molecular Biology
Juergen Roth, Christian Zuber, Sujin Park, Insook Jang, Yangsin Lee, Katarina Gaplovska Kysela, Valerie Le Fourn, Roger Santimaria, Bruno Guhl, Jin Won Cho
MOLECULES AND CELLS
(2010)
Article
Agronomy
Georgi Bonchev, Roman Dusinsky, Pavol Hauptvogel, Katarina Gaplovska-Kysela, Miroslav Svec
GENETIC RESOURCES AND CROP EVOLUTION
(2019)
Article
Agriculture, Multidisciplinary
Jana Spackova, Daniela Oliveira, Marek Puskar, Ivana Durovcova, Katarina Gaplovska-Kysela, Rui Oliveira, Andrea Sevcovicova
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2020)
Article
Chemistry, Analytical
Jakub Joniak, Henrieta Stankovicova, Juraj Filo, Katarina Gaplovska-Kysela, Vladimir Garaj, Marek Cigan
SENSORS AND ACTUATORS B-CHEMICAL
(2020)
Article
Genetics & Heredity
Andrea Sevcovicova, Jana Plava, Matej Gazdarica, Eva Szabova, Barbora Huraiova, Katarina Gaplovska-Kysela, Ingrid Cipakova, Lubos Cipak, Juraj Gregan
Summary: The study revealed that phosphorylation sites on the Swi5-Sfr1 complex contribute to its partially functional state during meiosis, but the functional relevance of this phosphorylation remains to be determined.
Article
Microbiology
Ivana Durovcova, Eduard Goffa, Zuzana Sestakova, Dominika Manikova, Katarina Gaplovska-Kysela, Miroslav Chovanec, Andrea Sevcovicova
Summary: BPA, a major component in common plastic products, acts as an endocrine disrupting compound with adverse physiological effects. High concentrations of BPA affect cell survival and increase intracellular oxidation, primarily in the mitochondrion, but acute exposure does not cause significant oxidative damage to DNA or proteins.
Article
Chemistry, Physical
Jakub Joniak, Henrieta Stankovicova, Simon Budzak, Milan Sykora, Katarina Gaplovska-Kysela, Juraj Filo, Marek Cigan
Summary: 3-aminocoumarins are introduced as turn-on pH probes under strongly acidic conditions and indicators capable of significantly improving yeast vacuolar lumen staining compared to the commercial CMAC derivatives.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Chemistry, Multidisciplinary
Martin Gaplovsky, Katarina Gaplovska-Kysela
Review
Cell Biology
Juergen Roth, Gary Hin-Fai Yam, Jingyu Fan, Kiyoko Hirano, Katarina Gaplovska-Kysela, Valerie Le Fourn, Bruno Guhl, Roger Santimaria, Tania Torossi, Martin Ziak, Christian Zuber
HISTOCHEMISTRY AND CELL BIOLOGY
(2008)
Article
Biotechnology & Applied Microbiology
Romana Vrzonova, Renata Toth, Barbara Sivakova, Anna Motovska, Katarina Gaplovska-Kysela, Peter Barath, Lubom Tomaska, Attila Gacser, Toni Gabaldon, Jozef Nosek, Martina Nebohacova
Summary: In this study, the function and localization of Oct1p, an ortholog of 3-oxoadipyl-CoA thiolase from Candida parapsilosis, was investigated, confirming its presence in mitochondria. Phylogenetic profiling of Oct1p revealed an intricate evolutionary pattern indicating multiple horizontal gene transfers among different fungal groups.
FEMS YEAST RESEARCH
(2021)