Article
Cell Biology
Takafumi Ogawa, Koji Masumura, Yuki Kohara, Muneyoshi Kanai, Tomoyoshi Soga, Yoshikazu Ohya, T. Keith Blackwell, Masaki Mizunuma
Summary: Methionine restriction can extend lifespan and delay aging-associated pathologies. Supplementation with S-adenosyl-L-homocysteine (SAH) can mimic the effects of methionine restriction by reducing methionine levels and activating AMP-activated protein kinase (AMPK), thereby extending lifespan.
Article
Biotechnology & Applied Microbiology
Zhong-Ce Hu, Chui-Mu Zheng, Yun-Chao Tao, Shu-Nan Wang, Yuan-Shan Wang, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: This study aimed to increase the accumulation of S-adenosyl-L-methionine (SAM) in Saccharomyces cerevisiae by promoting ATP availability. The results showed that the lack of gene sod1 benefited ATP generation and positively regulated SAM synthesis. Additionally, improving substrate assimilation further enhanced SAM production. The SAM concentration of strain Delta SOD1 was increased from 7.3 g L-1 to 10.1 g L-1 in a 5-L fermenter.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Hailong Chen, Xiaoqin Chai, Yan Wang, Jing Liu, Guohai Zhou, Pinghe Wei, Yuhe Song, Lingman Ma
Summary: Background: Saccharomyces cerevisiae is commonly used for the production of S-adenosyl-l-methionine (SAM), but glucose repression negatively affects its production. This study shows that deleting REG1 and overexpressing SNF1 can improve SAM production in S. cerevisiae by enhancing glucose utilization and glycolysis, promoting ethanol conversion, enhancing precursor amino acid biosynthesis, and extending yeast lifespan.
MICROBIAL CELL FACTORIES
(2022)
Review
Nutrition & Dietetics
Hammad Ullah, Ayesha Khan, Kannan R. R. Rengasamy, Alessandro Di Minno, Roberto Sacchi, Maria Daglia
Summary: Depression is a prevalent and serious health issue globally, and current treatment options are limited. SAMe and probiotics have shown potential therapeutic roles in depression by regulating microbial imbalance and reducing inflammation.
Article
Biochemistry & Molecular Biology
Mangal S. Kadam, V. L. S. Prasad Burra
Summary: Methylation plays a significant role in various diseases, and methyltransferases are important targets for drug development. However, the function of methyltransferases relies on the presence of SAM. This article provides a comprehensive analysis of SAM-receptor interactions and reveals crucial insights into the structure and function of SAM.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biotechnology & Applied Microbiology
Hailong Chen, Nianqing Zhu, Yan Wang, Xinxin Gao, Yuhe Song, Jia Zheng, Jiaping Peng, Xin Zhang
Summary: This study successfully increased the production of SAM in yeast by enhancing glycolysis through deletion of specific genes kcs1 and arg82. The strategy proved to be effective in improving SAM yield, demonstrating the potential for enhancing production of specific metabolites through metabolic engineering.
Review
Food Science & Technology
Adam Staniszewski, Monika Kordowska-Wiater
Summary: The paper discusses the concept of probiotic yeasts and their application in functional foods, emphasizing their positive impact on health, and pointing out future research directions.
Article
Biochemical Research Methods
Suryang Kwak, Bejan Mahmud, Gautam Dantas
Summary: This study presents a expandable and tunable transactivation system, validated in probiotic yeast Saccharomyces boulardii, allowing for precise control of functionality through the introduction of new target sequences. The system enhances orthogonality of transactivation through complementarity between promoter and scRNA, as well as binding specificity between scRNA and transcriptional activator. Additionally, control of transactivation is achieved via inducible promoter for activator expression, showing potential for precise therapeutic functions in engineered probiotic yeast.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ryuichi Iwasaki, Tomohiro Bito, Atsushi Ishihara, Fumio Watanabe, Yukinori Yabuta
Summary: SAM and SAH are important biochemical intermediates, with SAM being the major methyl donor in vivo. The SAM to SAH ratio is a marker of methylation capacity. Stable isotope-labeled SAM and SAH are used to measure this ratio with high sensitivity. We focused on the SAHH of Pyrococcus horikoshii OT3 and prepared recombinant P. horikoshii SAHH to efficiently produce labeled SAH.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Jun-Min Lee, Min-Ho Park, Bu-Soo Park, Min-Kyu Oh
Summary: This study achieved microbial production of S-methyl-methionine (SMM) by introducing a plant-derived MMT gene into engineered Saccharomyces cerevisiae sake K6.
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Food Science & Technology
Adam Staniszewski, Monika Kordowska-Wiater
Summary: One approach to maintaining a healthy microbiota in the human gastrointestinal tract is through the consumption of probiotics. This study examined the probiotic potential of yeast strains isolated from Polish wines. The tested strains belonged to six different species and were evaluated for their probiotic properties, safety, enzymatic activity, and antioxidant properties. The results showed that certain strains of H. uvarum, M. pulcherrima, and S. cerevisiae exhibited promising probiotic characteristics.
Article
Biotechnology & Applied Microbiology
Jungyeon Kim, Yu Eun Cheong, Sora Yu, Yong-Su Jin, Kyoung Heon Kim
Summary: The study constructed a yeast strain capable of metabolizing l-fucose in the gut, enhancing its metabolic activity. Through gene expression and metabolic modeling analysis, it was found that the yeast consumes l-fucose via a specific pathway. This study is significant for enhancing the metabolic activity of yeast and other probiotics in the gut.
MICROBIAL CELL FACTORIES
(2022)
Article
Food Science & Technology
Fernanda Cosme, Antonio Ines, Alice Vilela
Summary: In addition to traditional dairy products, innovative non-dairy products containing probiotics are becoming increasingly popular. These non-dairy products, by modulating the gut microbiota, have been shown to be beneficial for human health.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biotechnology & Applied Microbiology
Chang Dong, J. Carl Schultz, Wei Liu, Jiazhang Lian, Lei Huang, Zhinan Xu, Huimin Zhao
Summary: This study identified novel targets for enhancing SAM production through genome-scale engineering (GSE) and validated their effects in industrial yeast strains, leading to significant increases in SAM productivity. Transcriptomic and metabolomic analyses were conducted to investigate the molecular mechanisms for enhanced SAM accumulation.
METABOLIC ENGINEERING
(2021)
Article
Cell Biology
Renata Orlowska, Jacek Zebrowski, Janusz Zimny, Piotr Androsiuk, Piotr Tomasz Bednarek
Summary: This study used a structural equation modeling approach to analyze the effects of Cu(II) and Ag(I) ions, DNA methylation, and S-adenosyl-L-methionine on the regeneration efficiency of triticale through in vitro culture. The results revealed the relationships between these factors and provided a theoretical model for the biological phenomena associated with green plant regeneration efficiency.
Editorial Material
Ethics
Nicanor Pier Giorgio Austriaco
AMERICAN JOURNAL OF BIOETHICS
(2012)
Article
Biotechnology & Applied Microbiology
James J. Ritch, Shawn M. Davidson, Jared J. Sheehan, Nicanor O. P. Austriaco
FEMS YEAST RESEARCH
(2010)
Review
Biotechnology & Applied Microbiology
Su-Ju Lin, Nicanor Austriaco
FEMS YEAST RESEARCH
(2014)
Article
Multidisciplinary Sciences
James Cebulski, Joshua Malouin, Nathan Pinches, Vincent Cascio, Nicanor Austriaco
Article
Oncology
Nicanor Austriaco
FRONTIERS IN ONCOLOGY
(2012)
Article
Medical Ethics
Nicanor Pier Giorgio Austriaco
Summary: The article discusses the controversial use of COVID-19 vaccines derived from cell lines isolated from aborted fetal tissue. While most faithful Catholic moral theologians believe that this use is not intrinsically evil, they emphasize the importance of making decisions about these vaccines guided by prayer and prudent judgment.
Article
Medical Ethics
Nicanor Pier Giorgio Austriaco
Summary: This passage discusses the moral justifiability of self-amputation for survival in the Catholic tradition. The author proposes that a proper understanding of the principle of totality, which involves assessing the benefit/risk impact of surgical intervention on the well-being of the individual, can justify such acts.
Article
Ethics
Nicanor Pier Giorgio Austriaco
JOURNAL OF MEDICINE AND PHILOSOPHY
(2016)
Article
Cell Biology
Nicanor Pier Giorgio Austriaco