Review
Oncology
Yibo Guo, Juan Luo, Hailin Zou, Chenxin Liu, Liang Deng, Peng Li
Summary: This article provides an overview of the core components and upstream regulators of Hippo-YAP/TAZ signaling in mammals, and systematically summarizes the identified transcription factors or partners responsible for the downstream transcriptional output of YAP/TAZ in various cancers.
Review
Cell & Tissue Engineering
Juan Luo, Peng Li
Summary: The Hippo pathway, initially identified as a master regulator for cell proliferation and organ size control, has been found to also play important roles in development, tissue regeneration and homeostasis, inflammation, immunity, and cancer. YAP/TAZ, the downstream effectors of the Hippo pathway, act as coactivators and depend on other transcription factors to mediate their transcriptional outputs. This review provides an overview of the core components and regulations of the Hippo pathway in mammals, summarizes the identified transcriptional factors or partners responsible for the transcriptional output of YAP/TAZ in stem cells and differentiation, and discusses the potential applications and future directions based on these findings.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Oncology
Masao Saitoh
Summary: The epithelial-mesenchymal transition (EMT) is a process where epithelial cells transform into mesenchymal cells, which is associated with invasion, metastasis, and resistance to therapy in cancer. Transcription factors, such as ZEB1/ZEB2, SNAIL, and TWIST, play important roles in driving the EMT process. This review focuses on understanding the molecular mechanisms that sustain high levels of these transcription factors in aggressive carcinomas and their upregulation by transforming growth factor-β during cancer progression.
SEMINARS IN CANCER BIOLOGY
(2023)
Review
Cell Biology
Margaux Richter, Enzo Lalli, Carmen Ruggiero
Summary: The secreted protein augurin, derived from the tumor suppressor gene Ecrg4, was identified as a peptide hormone in the human proteome in 2007. Since then, numerous studies have been conducted to understand its structure, processing, and potential roles in various physiological and pathological processes. However, the molecular mechanisms and signaling pathways of augurin's biological effects remain poorly understood. This article provides a comprehensive overview of augurin-dependent signal transduction pathways, emphasizing the potential for therapeutic targeting of augurin and its derived peptides.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Neurosciences
Sarah Belhocine, Andre Machado Xavier, Felix Distefano-Gagne, Stephanie Fiola, Serge Rivest, David Gosselin
Summary: The study found that regulation of cell cycle gene expression in mouse microglia can be coordinated through two different mechanisms. One mechanism increases the expression of genes already expressed in quiescent microglia, regulated by Klf/Sp, Nfy, and Ets transcription factors; while another mechanism allows for de novo transcription of cell cycle genes, requiring additional regulatory input from Lin54 and E2f transcription factors.
Article
Biochemistry & Molecular Biology
Songhao Luo, Zihao Wang, Zhenquan Zhang, Tianshou Zhou, Jiajun Zhang
Summary: Gene expression in mammalian cells is highly variable and episodic, resulting in a series of discontinuous bursts of mRNAs. Single-cell RNA sequencing (scRNA-seq) provides an opportunity to understand these bursts on a genome-wide scale, but effective analytical methods are scarce. We developed an interpretable and scalable inference framework to study transcriptional burst kinetics and regulatory feedback in embryonic mouse fibroblast cells, and found that feedback regulations have significant impacts on burst frequencies and sizes.
NUCLEIC ACIDS RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Markus M. Kramer, Levin Lataster, Wilfried Weber, Gerald Radziwill
Summary: Biological signals are transduced and processed through a complex intracellular signaling network, leading to cell-specific responses. Optogenetic tools, based on genetically encoded light-sensing proteins, provide precise spatiotemporal control of signal transduction pathways in the absence of natural ligands, connecting light-regulated protein-protein interactions with the steering of signaling proteins. The engineering and application of optogenetic tools for the control of transmembrane receptors and their effector proteins allow for spatial and temporal control of signaling events.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Lili Gai, Yuting Zhu, Chun Zhang, Xianfang Meng
Summary: STAT plays a crucial role in various renal diseases by transducing signals from cytokines, chemokines, and growth factors. While most research has focused on the canonical pathways involving JAKs and STAT activation, exploring non-canonical pathways may offer novel therapeutic strategies.
Review
Biochemistry & Molecular Biology
Veronica Ruta, Vittoria Pagliarini, Claudio Sette
Summary: This review explores how signal transduction pathways regulate factors involved in RNA processing events and highlights the impact of various post-translational modifications on RNA-binding proteins. It also illustrates examples of how common signal transduction pathways can modulate biological processes through changes in RNA processing regulation.
Review
Biochemistry & Molecular Biology
Ambre Guillory, Sandrine Bonhomme
Summary: Research has shown that most known phytohormones play a role in moss development, with a focus on Physcomitrium patens. Synthesis and signaling pathways for classical hormones like auxins, cytokinins, and abscisic acid are best described in P. patens.
PLANT MOLECULAR BIOLOGY
(2021)
Review
Clinical Neurology
Xue Zhang, Fang Chen, Mingyue Sun, Nan Wu, Bin Liu, Xiangming Yi, Ruli Ge, Xueli Fan
Summary: Multiple sclerosis (MS) is an inflammatory and neurodegenerative disease that causes disability in young adults. The damage to myelin, oligodendrocytes, and axons is the characteristic feature of MS. Microglia play important roles in CNS surveillance, neurogenesis, synaptic refinement, and myelin pruning, but their continuous activation can contribute to neurodegeneration.
FRONTIERS IN NEUROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yoona Jung, Jooseong Kim, Sukyoung Kim, Shin Hye Chung, Jinhong Wie
Summary: Bioceramics, such as calcium-phosphate-based materials, are used in medical and dental implants for bone tissue replacement or repair. The effect of bioceramic sintering on intracellular signaling pathways is still unknown. This study analyzed the impact of sintering on osteoblast cell signaling pathways using sintered and non-sintered hydroxyapatite (HA) and beta-tricalcium phosphate (beta-TCP). The findings showed that sintering affects multiple signaling pathways, providing insights into bioceramic function and expanding their application scope in clinical settings.
Review
Medicine, Research & Experimental
Youchao Qi, Guozhen Duan, Guanghui Fan, Ning Peng
Summary: Lycium barbarum polysaccharide (LBP) is a major active ingredient in Lycium barbarum, which has beneficial effects through various signal pathways. While there have been many studies on LBP in physiology and pathology, there is a lack of reviews on its involvement in signal transduction pathways. This research aims to provide theoretical evidence for a comprehensive analysis of LBP mechanisms in clinical studies.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Francesca Aglialoro, Asena Abay, Nurcan Yagci, Minke A. E. Rab, Lars Kaestner, Richard van Wijk, Marieke von Lindern, Emile van den Akker
Summary: In this study, we found that PIEZO1 acts as a mechanosensor that, when transferred to orbital shaking, activates signaling cascades that are Ca2+-dependent, regulating various aspects of erythropoiesis. This suggests that there are direct physiological outcomes of mechanical stress on erythroblasts, as shown by the comparable activation of some Ca2+-dependent pathways induced by Yoda1 treatment and mechanical stress via orbital shaking.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Hanwei Du, Jiajia Chen, Haiying Zhan, Shen Li, Yusheng Wang, Wei Wang, Xiuli Hu
Summary: The calcium ion (Ca2+) acts as an important second messenger in plants, playing a crucial role in growth, development, and stress adaptation. Calcium-dependent protein kinases (CDPKs), acting as central Ca2+ sensor proteins, are widely present in plants. In maize, the response processes of ZmCDPKs to abiotic stresses have been well studied, and it has been found that they are regulated by various abiotic stresses and transmit signals to downstream molecules through protein interaction or phosphorylation, leading to cascade reactions and involvement in hormone and reactive oxygen signaling regulation. ZmCDPKs play an indispensable role in regulating maize growth, development, and stress responses. This review summarizes the roles of ZmCDPKs in integrating different signaling pathways to regulate maize's response to abiotic stress, providing insights into the molecular mechanisms of ZmCDPKs in maize's tolerance to abiotic stress and creating new opportunities for agricultural applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Allergy
Kyung Won Kim, Dong Yun Kim, Dankyu Yoon, Ka-Kyung Kim, Haerin Jang, Nathan Schoettler, Eun Gyul Kim, Mi Na Kim, Jung Yeon Hong, Jeom-Kyu Lee, Sangwoo Kim, Carole Ober, Heon Yung Gee, Myung Hyun Sohn
Summary: This study identified a new susceptibility gene, TNFSF15, and replicated associations at the 17q12-21 locus for childhood-onset asthma in Korean children with persistent asthma. The novel association may be mediated by reduced expression of serum TNFSF15 and loss of suppression of angiogenesis.
Editorial Material
Immunology
Seong Jin Choi, Dong-Uk Kim, Ji Yun Noh, Sangwoo Kim, Su-Hyung Park, Hye Won Jeong, Eui-Cheol Shin
CELLULAR & MOLECULAR IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yohan Yang, Eunyoung Kim, Sangwoo Kim
Summary: The study analyzed data from patients receiving immune checkpoint blockade treatment and found that HLA allelic copy loss was not significantly associated with treatment response rates, showing a better response in urothelial cancer. Regardless of HLA copy status, the proportion of cytotoxic immune cells in the patient's immune environment was correlated with treatment response, which was higher in the loss of heterozygosity group in the urothelial cohort.
Article
Multidisciplinary Sciences
Yoo-Jin Ha, Myung Joon Oh, Junhan Kim, Jisoo Kim, Seungseok Kang, John D. Minna, Hyun Seok Kim, Sangwoo Kim
Summary: This study provides a set of ultra-deep sequenced data for somatic mosaicism detection strategies based on cell line mixtures. By mimicking the cumulative nature of mosaic variant acquisition, the study reveals the inter-sample relationships and holds significance for optimizing detection strategies and developing algorithms.
Article
Cell & Tissue Engineering
Sun-Ho Lee, Jaewon Oh, Seung-Tae Lee, Dongju Won, Sangwoo Kim, Hyo-Kyoung Choi, Seok-Jun Kim, Hyunho Han, Minjae Yoon, Jong Rak Choi, Ho-Geun Yoon, Sahng Wook Park, Seok-Min Kang, Seung-Hyun Lee
Summary: Dilated cardiomyopathy (DCM) is a heart muscle disease that causes heart failure, and one of the most prevalent genetic causes is a protein-truncating variant in the Titin gene (TTNtv). Researchers have generated hiPSCs from patients who underwent heart transplantation due to DCM carrying a TTNtv mutation, and successfully differentiated them into cardiomyocytes.
STEM CELL RESEARCH
(2022)
Article
Cell & Tissue Engineering
Yun-Ji Cha, Sae-Bom Jeon, Jaewon Oh, Seung-Tae Lee, Sangwoo Kim, Hyoeun Kim, Jungyoon Choi, Hyo-Kyoung Choi, Dongju Won, Jong Rak Choi, Seok-Jun Kim, Sahng Wook Park, Seok-Min Kang, Seung-Hyun Lee
Summary: In this study, a human-induced pluripotent stem cell (hiPSC) line was generated from a patient with dilated cardiomyopathy (DCM). The hiPSC line showed normal karyotype and high expression of pluripotency markers, and its differentiation potential was confirmed by staining of three germ layer markers.
STEM CELL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Mi-Kyoung Seo, Hyundeok Kang, Sangwoo Kim
Summary: This study developed a novel method to predict microsatellite instability (MSI) in colorectal cancer (CRC) using transcriptomic profile and tumor microenvironment (TME). The method showed robustness and predictive power in predicting MSI status and has additional advantages compared to existing classifiers and other cancer types.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Lemessa Etana Bultum, Gemechu Bekele Tolossa, Doheon Lee
Summary: This study computationally investigated the therapeutic phytochemicals from Brucea antidysentrica for acute myeloid leukemia (AML). Through molecular docking and ADMET analysis, 12 phytochemicals were identified as candidate compounds for AML prevention and management.
Article
Genetics & Heredity
Ja Hye Kim, Shinwon Hwang, Hyeonju Son, Dongsun Kim, Il Bin Kim, Myeong-Heui Kim, Nam Suk Sim, Dong Seok Kim, Yoo-Jin Ha, Junehawk Lee, Hoon-Chul Kang, Jeong Ho Lee, Sangwoo Kim
Summary: In this study, a comprehensive analysis of low-level somatic mutations in multiple organ tissues was performed using deep whole-exome sequencing. The findings revealed that early clone-forming mutations shared between multiple organs had lower numbers but higher allele frequencies than late clone-forming mutations. These early and late clone-forming mutations also exhibited unique mutational signatures distinct from tumor-originated mutations. Late clone-forming mutations showed organ, tissue, and cell-type specificity in mutation counts, allele frequencies, and mutational signatures, with brain somatic mutations showing a bimodal occurrence and temporal-lobe-specific signature.
Article
Cell & Tissue Engineering
Sae-Bom Jeon, Hyoeun Kim, Kyeong-Hyeon Chun, Jaewon Oh, Chulan Kwon, Hyo-Kyoung Choi, Sangwoo Kim, Hyoung-Pyo Kim, In-Cheol Kim, Jung-Yoon Yoo, Sahng Wook Park, Seok-Min Kang, Seung-Hyun Lee
Summary: TNNT2 gene with p. Arg205Trp mutation is strongly linked to dilated cardiomyopathy (DCM). A human induced pluripotent stem cell (hiPSC) line, YCMi007-A, was generated from a DCM patient and showed high expression of pluripotent markers, normal karyotype, and differentiation into three germ layers. Therefore, YCMi007-A could be a valuable tool for investigating DCM.
STEM CELL RESEARCH
(2023)
Article
Biochemical Research Methods
Seunghyun Wang, Doheon Lee
Summary: In this study, two prognostic subgroups (BPS-LumA and WPS-LumA) of luminal-A breast cancer were identified using deep autoencoders and gene expressions. The prognostic difference between the two subgroups was validated using external datasets. The study also found that latent features were superior to gene expression profiles in discovering the prognostic subgroups.
PLOS COMPUTATIONAL BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yousang Jo, Maree J. Webster, Sanghyeon Kim, Doheon Lee
Summary: This study proposes a stepwise deep learning technique (SLEM) with multi-precision data to explore the effects of SNP combinations on schizophrenia. The technique utilizes two levels of precision data and identifies effective SNP interactions from a large space of possible combinations. The results show that the extracted SNP combinations have higher accuracy than single SNPs and can be validated in an independent dataset. The learned networks also provide interpretations of molecular and cellular interactions of SNP combinations towards schizophrenia etiology.
BRIEFINGS IN FUNCTIONAL GENOMICS
(2023)
Article
Biochemical Research Methods
Jaesub Park, Sangyeon Lee, Kwansoo Kim, Jaegyun Jung, Doheon Lee
Summary: In this study, a computational approach called LAPINE is proposed for large-scale prediction of ADR-related proteins. The results show that LAPINE is capable of predicting ADR-related proteins with high reliability, providing insights into the mechanism of ADRs and aiding in prevention. The source code of LAPINE is available on GitHub and Figshare.
Article
Biochemistry & Molecular Biology
Se-Young Jo, Namki Hong, Seunghyun Lee, Jong Ju Jeong, Jeongsoo Won, Jiho Park, Gi Jeong Kim, Sang Kyum Kim, Sangwoo Kim, Yumie Rhee
Summary: Genomic and transcriptomic profiling of parathyroid tissues revealed multiple two-hit mutation patterns in the CDC73 gene, suggesting potential therapeutic targets. Transcriptomic analysis identified upregulated expression of E2F targets, KRAS and TNF-alpha signaling, and epithelial-mesenchymal transition pathways in parathyroid carcinomas. A molecular classification model based on carcinoma-specific genes could effectively distinguish between carcinomas, adenomas, and normal tissues, with potential clinical utility.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
Meeting Abstract
Oncology
Seon-Jin Yoon, Ran Joo Choi, Yoojung Oh, Se-Young Cho, Hyun Jung Kim, Joo Ho Lee, Eunchae Yeo, Jihwan Yoo, Ju Hyung Moon, Eui Hyun Kim, Se Hoon Kim, Huyn Seok Kim, Jong Hee Chang, Sangwoo Kim, Hoon Kim, Jeong Ho Lee, Seok-Gu Kang