Review
Cell Biology
Parinaz Ahangar, Allison J. Cowin
Summary: The restoration of an intact epidermal barrier after wound injury is a complex physiological process regulated by the cytoskeleton and the formin family of proteins, which play essential roles in multiple important processes involved in wound repair.
Review
Cell Biology
Susumu Antoku, Thomas U. Schwartz, Gregg G. Gundersen
Summary: In this review, the FHOD formin family is discussed, with a particular focus on their newly discovered role as critical links in nuclear mechanotransduction. The FHOD family in vertebrates consists of two structurally related proteins, FHOD1 and FHOD3, which have similar biochemical properties and potentially redundant functions. FHODs have unique characteristics among formins, including strong F-actin bundling activity and weak actin polymerization activity, and they are regulated by phosphorylation and association with nuclear envelope proteins. Recent studies have shown that FHODs play a role in reinforcing the LINC complex and connecting to perinuclear actin, contributing to the mechanical functions of tissues such as striated muscle. Human genetic studies have also linked FHOD3 variants to dilated and hypertrophic cardiomyopathies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Xuemeng Shi, Daijiao Tang, Yifan Xing, Shuangshuang Zhao, Changyuan Fan, Jin Zhong, Yanqin Cui, Kun Shi, Yaming Jiu
Summary: The study found that both the mechanosensitive actin cytoskeleton and caveolae play important roles in cell migration, morphogenesis, and vesicular trafficking. Results indicate that actin-associated formins are essential for maintaining the dynamic organization of cytoplasmic CAV-1 and its sensitivity upon mechanical challenge. These formins act as a safety valve in dampening excessive tension on CAV-1 and safeguarding it against mechanical damage.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Eric J. Schmidt, Salome Funes, Jeanne E. McKeon, Brittany R. Morgan, Sivakumar Boopathy, Lauren C. O'Connor, Osman Bilsel, Francesca Massi, Antoine Jegou, Daryl A. Bosco
Summary: The study identified differential binding between ALS-linked PFN1 variants and formin proteins, resulting in enhanced actin assembly. Molecular dynamics simulations revealed mutation-induced changes in internal dynamics that could lead to increased flexibility of the ternary complex.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Leticia Labat-de-Hoz, Laura Comas, Armando Rubio-Ramos, Javier Casares-Arias, Laura Fernandez-Martin, David Pantoja-Uceda, M. Teresa Martin, Leonor Kremer, M. Angeles Jimenez, Isabel Correas, Miguel A. Alonso
Summary: This study reveals the structure and function of the N-terminal extension of the INF2 protein, which is involved in the activation of INF2 by interacting with CaM.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Dennis Frank, Jessica Christel Moussie, Svenja Ulferts, Lina Lorenzen, Carsten Schwan, Robert Grosse
Summary: Vesicle trafficking is important for tumor progression, and this study reveals the role of Formin-like 2 (FMNL2) in the trafficking and secretion of Angiopoietin-like 4 (ANGPTL4). The phosphorylation of FMNL2 by protein kinase C (PKC) downstream of transforming growth factor beta (TGF beta) is required for cancer cell invasion and ANGPTL4 vesicle trafficking and secretion. Additionally, super resolution microscopy shows that ANGPTL4 trafficking is actin-dependent, and FMNL2 polymerizes actin at ANGPTL4-containing vesicles, associated with Rab8a and myosin Vb. This mechanism may play an important role in regulating cancer cell metastasis and tumor progression.
Review
Plant Sciences
Min Diao, Shanjin Huang
Summary: Cell-to-cell communication in plants is mediated by plasmodesmata with regulated permeability. Actin cytoskeleton is involved in regulating the permeability, with recent studies on PD-localized formin proteins shedding light on their role and mechanism in intercellular trafficking. This mini-review article describes the progress in understanding these mechanisms.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Hiroki Sakuta, Naoki Nakatani, Takayuki Torisawa, Yutaka Sumino, Kanta Tsumoto, Kazuhiro Oiwa, Kenichi Yoshikawa
Summary: Water phase separation in cells can lead to mechanical motion by entrapping protein motors and generating vortex flow inside cell-sized droplets. This study reveals that the water phase separation interface can drive translational motion of droplets by spontaneously entrapping kinesins and microtubules and generating vortex flow. The findings highlight the importance of water phase separation in both chemical and mechanical processes.
COMMUNICATIONS CHEMISTRY
(2023)
Editorial Material
Biochemistry & Molecular Biology
Michael J. Deeks
Summary: The study highlights the importance of microbe-plant cell interactions in shaping plant cell morphology and function, particularly demonstrating how a specialized formin protein facilitates nitrogen-fixing bacteria colonization in legumes.
Article
Pharmacology & Pharmacy
Santimoy Sen, Sheetal Lagas, Abhishek Roy, Hemant Kumar
Summary: Cells rely on a balanced cytoskeleton to maintain cellular integrity and regulate signaling pathways. Dysregulation of the cytoskeleton can lead to various pathological conditions, and targeting cytoskeletal dynamics may provide new therapeutic approaches for these diseases.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Developmental Biology
Su Deng, Ruth L. Silimon, Mridula Balakrishnan, Ingo Bothe, Devin Juros, David B. Soffar, Mary K. Baylies
Summary: The protein Dia plays a critical role in regulating sarcomere size by interacting with FliI, ensuring normal muscle function. These actin regulatory factors generate uniformly sized sarcomeres tuned for muscle contractions required for flight.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Physical
Vandana Yadav, Manoj Gopalakrishnan
Summary: The study focuses on the velocity-force relation for a Brownian ratchet in extreme limits of fast and slow barrier diffusion, deriving different V-F relations and the impact of filament number on velocity. Mean-field argument predicts equal load sharing in both limits, but different scaling behavior of velocity with filament number.
EUROPEAN PHYSICAL JOURNAL E
(2022)
Article
Biochemistry & Molecular Biology
Lina Lorenzen, Dennis Frank, Carsten Schwan, Robert Grosse
Summary: FMNL2 is upregulated in several cancers and plays important roles in cell migration, invasion, cell-cell adhesion and filopodia formation. Using structured illumination microscopy, it was found that FMNL2 promotes rapid and highly dynamic filopodia formation in epithelial cells while remaining on the tip of the growing filopodia. This filopodia tip localization depends on its N-terminal myristoylation and serine 1072 phosphorylation within the diaphanous-autoregulatory domain (DAD) by protein kinase C (PKC) alpha.
Article
Multidisciplinary Sciences
Jianjiang Hu, Xiaowei Gong, Staffan Stromblad
Summary: Cells probe their microenvironment using membrane protrusion-retraction cycles. Spatial and temporal coordination between Rac1 and RhoA GTP-binding activities play critical roles in initiating and reinforcing protrusions and retractions. Rac1-GTP levels increase before protrusions, while RhoA-GTP levels increase before retractions. Rac1-GTP nadirs and peaks coincide with maximal edge velocity of local membrane protrusions and retractions, followed by declined edge velocity. The alteration of local Rac1-GTP precedes the alteration of traction force. Through optogenetic Rac1-GTP perturbations, we demonstrate the fundamental role of Rac1 in restricting the size and durability of protrusions and retractions, possibly through controlling traction forces.
Article
Neurosciences
Tanushree Kundu, Sooraj Siva Das, Lisas K. Sewatkar, Divya S. Kumar, Dhriti Nagar, Aurnab Ghose
Summary: The study found that Fmn2 regulates the formation of axonal collateral protrusions in chick and zebrafish, localizing to the protrusion-initiating axonal F-actin patches and regulating their lifetime and size. The F-actin nucleation activity of Fmn2 is necessary for patch stability but not for patch formation. Fmn2 insulates the F-actin patches from disassembly by ADF, promoting long-lived, larger patches that can initiate axonal protrusions.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Thermodynamics
Veronika Takacs-Kollar, Miklos Nyitrai, Denes Lorinczy, Gabor Hild
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2017)
Article
Thermodynamics
Veronika Takacs-Kollar, Miklos Nyitrai, Denes Lorinczy, Gabor Hild
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2018)
Article
Neurosciences
Tams Kovacs-Oller, Gabor Debertin, Marton Balogh, Alma Ganczer, Jozsef Orban, Miklos Nyitrai, Lajos Balogh, Orsolya Kantor, Bela Volgyi
FRONTIERS IN CELLULAR NEUROSCIENCE
(2017)
Article
Pharmacology & Pharmacy
Aliz Szabo, Katalin Sumegi, Katalin Fekete, Eniko Hocsak, Balazs Debreceni, Gyorgy Setalo, Krisztina Kovacs, Laszlo Deres, Andras Kengyel, Dominika Kovacs, Jozsef Mandl, Miklos Nyitrai, Mark A. Febbraio, Ferenc Gallyas, Balazs Sumegi
BIOCHEMICAL PHARMACOLOGY
(2018)
Article
Biophysics
Nikolett Kis-Bicskei, Balint Becsi, Ferenc Erdodi, Robert C. Robinson, Beata Bugyi, Tamas Huber, Miklos Nyitrai, Gabor Csaba Talian
BIOPHYSICAL JOURNAL
(2018)
Article
Spectroscopy
Yin Li, Andras Lukacs, Sandor Bordacs, Janos Moczar, Miklos Nyitrai, Janos Hebling
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2018)
Article
Multidisciplinary Sciences
David Szatmari, Bo Xue, Balakrishnan Kannan, Leslie D. Burtnick, Beata Bugyi, Miklos Nyitrai, Robert C. Robinson
Article
Chemistry, Analytical
Henriett Halasz, Ali Reza Ghadaksaz, Tamas Madarasz, Krisztina Huber, Gabor Harami, Eszter Angela Toth, Aniko Osteikoetxea-Molnar, Mihaly Kovacs, Zsolt Balogi, Miklos Nyitrai, Janos Matko, Edina Szabo-Meleg
METHODS AND APPLICATIONS IN FLUORESCENCE
(2018)
Article
Thermodynamics
P. Maroti, P. Varga, A. Ferencz, Z. Ujfalusi, M. Nyitrai, D. Lorinczy
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2019)
Article
Materials Science, Multidisciplinary
Peter Maroti, Peter Varga, Hajnalka Abraham, Gyorgy Falk, Tamas Zsebe, Zoltan Meiszterics, Sandor Mano, Zoltan Csernatony, Szilard Rendeki, Miklos Nyitrai
MATERIALS RESEARCH EXPRESS
(2019)
Article
Materials Science, Multidisciplinary
P. Varga, D. Lorinczy, L. Toth, A. Pentek, M. Nyitrai, P. Maroti
MATERIALS RESEARCH EXPRESS
(2019)
Article
Thermodynamics
P. Maroti, B. Kocsis, A. Ferencz, M. Nyitrai, D. Lorinczy
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2020)
Article
Multidisciplinary Sciences
Adam Jonatan Tengolics, Gergely Szarka, Alma Ganczer, Edina Szabo-Meleg, Miklos Nyitrai, Tamas Kovacs-Oller, Bela Volgyi
SCIENTIFIC REPORTS
(2019)
Article
Multidisciplinary Sciences
Kristof Karadi, Sofia M. Kapetanaki, Katalin Raics, Ildiko Pecsi, Robert Kapronczai, Zsuzsanna Fekete, James N. Iuliano, Jinnette Tolentino Collado, Agnieszka A. Gil, Jozsef Orban, Miklos Nyitrai, Greg M. Greetham, Marten H. Vos, Peter J. Tonge, Stephen R. Meech, Andras Lukacs
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Eszter A. Toth, Adam Oszvald, Maria Peter, Gabor Balogh, Aniko Osteikoetxea-Molnar, Tamas Bozo, Edina Szabo-Meleg, Miklos Nyitrai, Imre Derenyi, Miklos Kellermayer, Toshiyuki Yamaji, Kentaro Hanada, Laszlo Vigh, Janos Matko
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2017)