Article
Biochemistry & Molecular Biology
Martina Casarin, Tiago Moderno Fortunato, Saima Imran, Martina Todesco, Deborah Sandrin, Giulia Borile, Ilaria Toniolo, Massimo Marchesan, Gino Gerosa, Andrea Bagno, Filippo Romanato, Emanuele Luigi Carniel, Alessandro Morlacco, Fabrizio Dal Moro
Summary: Bladder cancer is a common malignancy that often requires bladder removal surgery for treatment. However, the use of autologous intestine segments can lead to complications and affect patients' quality of life. This study aims to develop a bio-engineered material as a replacement, and the preliminary evaluation shows promising results for decellularized porcine small intestinal submucosa as a suitable candidate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Charlot Philips, Lisanne Terrie, Lieven Thorrez
Summary: A review is presented on the progress and challenges in skeletal muscle decellularization, discussing different decellularization methods and applications of the scaffolds. Decellularized skeletal muscle has been primarily studied for regenerative purposes, focusing on tissue-specific morphological features and biochemical cues for muscle regeneration. However, this review expands the potential applications of decellularized skeletal muscle to demonstrate its versatility as a biomaterial.
Review
Biochemistry & Molecular Biology
Beom-Seok Kim, Jeong-Uk Kim, Kyoung-Ha So, Nathaniel S. Hwang
Summary: Supercritical fluid-based extraction technologies are increasingly utilized for high purity extract products in food industries, and have been transformed for use in tissue engineering and biomedical applications. Supercritical fluid-based decellularization protocols offer advantages over conventional methods by preserving extracellular matrix components and structures, making them ideal for regenerative medicine applications.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Engineering, Biomedical
Bin Wang, Qinglai Tang, Qian Yang, Mengmeng Li, Shiying Zeng, Xinming Yang, Zian Xiao, Xinying Tong, Lanjie Lei, Shisheng Li
Summary: Due to their unique properties such as low immunogenicity, biomimetic internal environment, tissue-and organ-like physicochemical properties, and functionalization potential, decellularized extracellular matrix (dECM) materials have gained considerable attention in tissue engineering. This review provides an overview of the composition of extracellular matrices and their role in stem-cell differentiation, discusses the advantages and disadvantages of existing decellularization techniques, and presents methods for the functionalization and characterization of decellularized scaffolds. Additionally, the review discusses the progress and shortcomings of using dECMs in cartilage, skin, nerve, and muscle repair, as well as the transplantation or regeneration of different whole organs, and examines the future prospects of refunctionalization in tissue and organ repair. It aims to systematize the application of functionalized dECMs in tissue/organ transplantation and keep researchers informed about the latest progress in dECM usage.
MATERIALS TODAY BIO
(2023)
Article
Biochemistry & Molecular Biology
Ilya Klabukov, Dmitri Atiakshin, Evgenia Kogan, Michael Ignatyuk, Mikhail Krasheninnikov, Nickolay Zharkov, Anna Yakimova, Vyacheslav Grinevich, Pavel Pryanikov, Vladimir Parshin, Dmitry Sosin, Andrey A. Kostin, Peter Shegay, Andrey D. Kaprin, Denis Baranovskii
Summary: There is a lack of comprehensive research on immune responses to xenogeneic tissue-engineered grafts. This study aims to investigate the immunological features of tracheal repair using human xenogeneic scaffolds modified by laser radiation in rabbits. The study found that seeding xenogeneic cartilage with chondrocytes resulted in changes in immune reactions and plasma cell infiltration during implantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Danni Gong, Fei Yu, Meng Zhou, Wei Dong, Dan Yan, Siyi Zhang, Yan Yan, Huijing Wang, Yao Tan, Ying Chen, Bei Feng, Wei Fu, Yao Fu, Yang Lu
Summary: dcECM hydrogels derived from porcine ears through enzymatic digestion show good biocompatibility for delivering chondrocytes and forming subcutaneous cartilage in vivo. The structural and gelation kinetics of the hydrogels vary with ECM concentrations, with 10 mg/ml hydrogels supporting adhesion and proliferation of chondrocytes in vitro. In vivo, 10 mg/ml dcECM hydrogel grafts exhibit similar qualities to native cartilage, indicating their potential for tissue engineering applications in ear cartilage regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Greta Ionela Barbulescu, Florina Maria Bojin, Valentin Laurentiu Ordodi, Iacob Daniel Goje, Andreea Severina Barbulescu, Virgil Paunescu
Summary: Cardiovascular diseases are the leading cause of global mortality. Decellularized cardiac extracellular matrix (dECM) has shown promise in tissue-regenerative medicine, but proper assessment of tissue composition and recellularization with organ-specific cells remain challenging.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Engineering, Biomedical
Tanveer Ahmad Mir, Makoto Nakamura, Shinji Sakai, Shintaroh Iwanaga, Shadil Ibrahim Wani, Alaa Alzhrani, Kenichi Arai, Bilal Ahmed Mir, Shadab Kazmi, Abdullah M. Assiri, Dieter C. Broering
Summary: The shortage of compatible liver donors and the increasing number of potential recipients have prompted scientists to explore alternate methods for providing tissue/organ substitutes from bioengineered sources. Hepatic tissue engineering has made remarkable progress in the past two decades by combining interdisciplinary research approaches. Three-dimensional (3D) bioprinting has emerged as a promising tool with the potential to fabricate volumetric liver tissue/organ equivalents using printable bioinks composed of hydrous microenvironments, living cells, and associated supplements. This review discusses the future utility of bioprinting technology and the use of decellularized biomaterials as bioink substrates for liver tissue engineering.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Review
Engineering, Biomedical
Lillian Yuxian Lim, Shirley Suet Lee Ding, Padmalosini Muthukumaran, Swee Hin Teoh, Yexin Koh, Adrian Kee Keong Teo
Summary: Pancreas or islet transplantation is the reliable treatment for Type I diabetes patients, but the shortage of viable pancreata limits its use. Organ decellularization and recellularization offer a potential alternative source of donor organs. Studies on decellularization and recellularization of rodent, porcine, and human pancreata show promise for generating usable decellularized pancreas scaffolds. This review discusses the latest advances in pancreas decellularization, recellularization, and revascularization, as well as clinical considerations and future work needed for clinical translation of bioengineered pancreata.
ACTA BIOMATERIALIA
(2023)
Article
Biotechnology & Applied Microbiology
Beu P. Oropeza, Jason R. Adams, Michael E. Furth, Jack Chessa, Thomas Boland
Summary: Bioprinting is an emerging technique that can be used to engineer tissue or create in vitro organ models. However, many bioprinted tissues lack the biochemical complexity of native extracellular matrix, resulting in inferior mechanical properties. The combination of decellularization and bioprinting processes has the potential to improve cellular environment and mechanical characteristics for better outcomes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Nanoscience & Nanotechnology
My Thi Ngoc Nguyen, Ha Le Bao Tran
Summary: The study aimed to prepare injectable acellular adipose matrix (AAM) through delipidating and decellularizing, showing that mechanical disruption improved fat removal efficiency and the decellularization strategy was effective. AAM powder was non-toxic to human adipose derived stem cells and supported cell migration and proliferation.
JOURNAL OF SCIENCE-ADVANCED MATERIALS AND DEVICES
(2021)
Article
Pharmacology & Pharmacy
Katja Jansen, Marianna Evangelopoulou, Carla Pou Casellas, Sarina Abrishamcar, Jitske Jansen, Tina Vermonden, Rosalinde Masereeuw
Summary: Tissue decellularization of plant tissues holds potential for organ replacement strategies, but recellularization is currently limited by anatomical constraints. Use of decellularized plant scaffolds for kidney tubule engineering is hindered by gradual tissue disintegration and insufficient diffusion capacity. Further research is needed to enhance capabilities by integrating plant-derived structures with other tissue engineering technologies.
Editorial Material
Chemistry, Multidisciplinary
Neill J. Turner
Summary: This Special Issue explores the various applications of extracellular matrix (ECM) in regenerative medicine, including manufacturing scaffold materials, understanding the impact of changes in ECM composition on disease development, and utilizing decellularization techniques to develop tissue-derived ECM scaffolds. Overall, the articles in this issue cover a wide range of research and applications of ECM in the field of regenerative medicine.
APPLIED SCIENCES-BASEL
(2021)
Review
Immunology
Alok Shiomurti Tripathi, Magdi E. A. Zaki, Sami A. Al-Hussain, Bidhyut Kumar Dubey, Prabhjot Singh, Laiba Rind, Rajnish Kumar Yadav
Summary: Biomaterials are widely used in various medical applications, but their introduction into the body can cause immune responses. The immune system recognizes natural biomaterials as foreign substances, activating immune cells and leading to inflammation. The surface properties and released immunomodulatory factors of biomaterials can influence immune cell behavior, and a better understanding of this interaction can help design improved biomaterials.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Cell & Tissue Engineering
Hoda Elkhenany, Azza El-Derby, Mohamed Abd Elkodous, Radwa A. Salah, Ahmed Lotfy, Nagwa El-Badri
Summary: The amniotic membrane (Amnio-M) has multiple applications in regenerative medicine, serving as a biocompatible scaffold, a source of stem cells and growth factors, and a nanoreservoir for drug delivery. Its use has evolved from simple sheet applications to more advanced forms, and nanotechnology is playing a role in expanding its applications in tissue engineering and clinical settings.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Almira Zada, Yuying Zhao, Danny Halim, Jonathan Windster, Herma C. van der Linde, Jackleen Glodener, Sander Overkleeft, Bianca M. de Graaf, Robert M. Verdijk, Alice S. Brooks, Iain Shepherd, Ya Gao, Alan J. Burns, Robert M. W. Hofstra, Maria M. Alves
Summary: Long FLNA isoform plays a crucial role in intestinal development and function, and its loss may result in myopathic form of CIPO.
HUMAN MOLECULAR GENETICS
(2023)
Article
Biochemical Research Methods
Laween Meran, Lucinda Tullie, Simon Eaton, Paolo De Coppi, Vivian S. W. Li
Summary: The authors present a protocol for bioengineering human intestinal mucosal grafts using patient-derived materials. This technology has significant potential for treating intestinal failure and studying human gastrointestinal diseases.
Letter
Pediatrics
Alexandra Stewart, Christina H. Smith, Jo Wray
JOURNAL OF PEDIATRIC SURGERY
(2023)
Article
Public, Environmental & Occupational Health
Maria Peppa, Bianca L. De Stavola, Stavros Loukogeorgakis, Ania Zylbersztejn, Ruth Gilbert, Paolo De Coppi
Summary: This study utilized national birth cohort data in England to compare the birth prevalence, relationship with maternal age, and 1-year mortality rates of different subtypes of congenital diaphragmatic hernia (CDH). The results showed that the risk of complex CDH was associated with maternal age, and the 1-year mortality rate for complex CDH was slightly higher than for isolated CDH.
PAEDIATRIC AND PERINATAL EPIDEMIOLOGY
(2023)
Article
Critical Care Medicine
Gareth A. L. Jones, Simon Eaton, Michael Orford, Samiran Ray, Daisy Wiley, Padmanabhan Ramnarayan, David Inwald, Michael P. W. Grocott, Michael Griksaitis, John Pappachan, Lauran R. O'Neill, Paul A. Mouncey, David M. Harrison, Kathryn J. Rowan, Mark Peters
Summary: The study aimed to compare the effects of conservative oxygenation targets and liberal oxygenation targets on the oxidative status of pediatric patients in the intensive care unit. The results showed that the conservative oxygenation group had higher levels of urinary nitrate/nitrite and lower expression of hypoxia-inducible factor (HIF)-1a mRNA in blood.
PEDIATRIC CRITICAL CARE MEDICINE
(2023)
Article
Pediatrics
Cunera M. C. de Beaufort, Dalia Aminoff, Ivo de Blaauw, Celia Cretolle, Jens Dingemann, Natalie Durkin, Wout F. J. Feitz, JoAnne Fruithof, Caterina Grano, Carmen Mesas Burgos, Nicole Schwarzer, Graham Slater, Tutku Soyer, Cristiano Violani, Rene Wijnen, Paolo de Coppi, Ramon R. Gorter
Summary: The aim of this international study was to assess the current status of transition of care (TOC) and adult care (AC) programs for patients with anorectal malformations (ARM) and Hirschsprung disease (HD). The results showed that less than 50% of the participating centers had installed these programs, and there was considerable heterogeneity in the content and implementation of the programs. Sharing best practice examples and considering local and national healthcare programs may improve the continuity of care in the future.
JOURNAL OF PEDIATRIC SURGERY
(2023)
Article
Acoustics
S. Vergote, F. R. De Bie, J. M. N. Duffy, J. Bosteels, A. Benachi, B. Power, F. Meijer, H. L. Hedrick, C. J. Fernandes, I. K. M. Reiss, P. De Coppi, K. P. Lally, J. A. Deprest
Summary: A core outcome set for evaluating perinatal interventions in congenital diaphragmatic hernia was developed with the input of stakeholders, standardizing the selection and definition of outcomes and facilitating future research to guide clinical practice.
ULTRASOUND IN OBSTETRICS & GYNECOLOGY
(2023)
Article
Multidisciplinary Sciences
Anna Urciuolo, Giovanni Giuseppe Giobbe, Yixiao Dong, Federica Michielin, Luca Brandolino, Michael Magnussen, Onelia Gagliano, Giulia Selmin, Valentina Scattolini, Paolo Raffa, Paola Caccin, Soichi Shibuya, Dominic Scaglioni, Xuechun Wang, Ju Qu, Marko Nikolic, Marco Montagner, Gabriel L. Galea, Hans Clevers, Monica Giomo, Paolo De Coppi, Nicola Elvassore
Summary: This study develops a hydrogel-in-hydrogel bioprinting approach to dynamically control the growth landscape of three-dimensional cell cultures. The technique enables modulation of hydrogel characteristics to guide neural axon directionality, control cell migration in cancer organoids, and promote cell polarity in liver and small intestinal organoids, as well as control lung tip bifurcation.
NATURE COMMUNICATIONS
(2023)
Article
Pediatrics
Amulya Saxena, Annika Mutanen, Ramon Gorter, Andrea Conforti, Pietro Bagolan, Paolo De Coppi, Tutku Soyer
Summary: This study reviewed the literature on the management of neonatal ovarian simple cysts and formulated a consensus statement based on current evidence and peer practice. Although there is limited data on the potential benefits and complications of prenatal ultrasound-guided aspiration, neonates after such procedures should be followed for 6 months. Neonates with simple ovarian cysts larger than 4 cm should undergo surgical interventions within the first two weeks of life, with laparoscopic cyst aspiration and fenestration using bipolar instruments as the preferred approach. Ultrasound follow-up should be done at 3 months after surgical intervention and every 3 to 4 months with the conservative approach until 1 year.
EUROPEAN JOURNAL OF PEDIATRIC SURGERY
(2023)
Article
Medicine, General & Internal
Ravi Jagatia, Ewald J. Doornebal, Una Rastovic, Nicola Harris, Moyosoreoluwa Feyide, Anabel Martinez Lyons, Rosa Miquel, Yoh Zen, Ane Zamalloa, Farooq Malik, Andreas Prachalias, Krishna Menon, Luke Boulter, Simon Eaton, Nigel Heaton, Sandra Phillips, Shilpa Chokshi, Elena Palma
Summary: In this study, patient-specific immunocompetent disease models of primary liver cancer (PLC) were created using precision-cut tissue slices (PCTS) derived from resected tumors, which effectively captured the multifaceted and intricate heterogeneity of the tumor and its microenvironment. The PCTS model maintained tissue architecture and tumor-specific immunophenotype, making it a robust preclinical tool for investigating immunotherapeutic drug efficacy and non-responsiveness.
Article
Pediatrics
Athanasios Tyraskis, Joseph Davidson, Jennifer Billington, Simon Blackburn, Joseph Curry, Dhanya Mullassery, Stefano Giuliani, Simon Eaton, Kate Cross, Paolo De Coppi
Summary: The purpose of this study was to identify markers of previous ovarian torsion and assess the outcomes according to ultrasound appearance and operative management. The study found that simple cysts have a higher rate of spontaneous regression and ovarian preservation, while complex cysts are more likely to require operative treatment. The presence of fluid-debris level in complex cysts is significantly associated with ovarian loss. Therefore, ultrasound examination and operative treatment are crucial for identifying and managing ovarian torsion.
PEDIATRIC SURGERY INTERNATIONAL
(2023)
Meeting Abstract
Rheumatology
Meredyth G. L. L. Wilkinson, Dale Moulding, Thomas C. R. McDonnell, Mi-Chael Orford, Chris Wincup, Joanna Ting, Georg Otto, Daniel Kelberman, Sergi Castellano, Simon Eaton, Claire T. Deakin, Elizabeth C. Rosser, Lucy R. Wedderburn
CLINICAL AND EXPERIMENTAL RHEUMATOLOGY
(2023)
Article
Surgery
Omid Madadi-Sanjani, Joachim F. Kuebler, Julia Brendel, Soeren Wiesner, Annika Mutanen, Simon Eaton, Anja Domenghino, Pierre-Alain Clavien, Benno M. Ure
Summary: To address the inconsistent definitions of surgical complications and unexpected events, a multidisciplinary expert group modified the Clavien-Dindo classification and introduced the Clavien-Madadi classification, which includes organizational and management errors. Unexpected events were documented in a cohort of 17,502 children undergoing surgery and compared with procedure complexity. The results showed that the Clavien-Madadi classification identified additional events and had a higher correlation with procedure complexity.
BRITISH JOURNAL OF SURGERY
(2023)
Letter
Surgery
Barbora Patkova, Anna Svenningsson, Markus Almstroem, Jan F. Svensson, Staffan Eriksson, Tomas Wester, Simon Eaton
Meeting Abstract
Surgery
F. Chang, A. Vaitkute, M. Attrill, S. Chong, H. Mahdi, P. Blair, A. Salama, C. Mauri, S. Eaton, M. Bajaj-Elliott, A. Pesenacker, R. Motallebzadeh
BRITISH JOURNAL OF SURGERY
(2023)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.
