Article
Engineering, Biomedical
Bin Wang, Wei Liu, Jiao Jiao Li, Senlin Chai, Dan Xing, Hongsheng Yu, Yuanyuan Zhang, Wenjin Yan, Zhihong Xu, Bin Zhao, Yanan Du, Qing Jiang
Summary: In the treatment of osteoarthritis, delivering low doses of MSCs through microcarriers can achieve similar reparative effects as repeated high doses. Mechanistic investigations reveal that 3D growth of MSCs reduces inflammatory factors, enhances early chondrogenesis and extracellular matrix interactions, and maintains the phenotype of co-cultured chondrocytes.
BIOACTIVE MATERIALS
(2022)
Review
Biochemistry & Molecular Biology
David A. Hart, Norimasa Nakamura
Summary: After injuries to musculoskeletal tissues, an inflammatory response occurs to promote endogenous repair, but it often results in functionally inferior scar tissue. Injuries in adults no longer have the anabolic processes that contribute to growth and maturation, which can lead to degenerative conditions such as osteoarthritis. Researchers have been exploring cellular therapies, such as Platelet-rich Plasma and mesenchymal stem/medicinal signaling cells, to enhance tissue repair and regeneration. However, implanting these cells or engineered constructs into an inflammatory adult environment may compromise their potential for regeneration. Therefore, it may be necessary to neutralize the inflammatory environment and create an anabolic environment for optimal outcomes.
Article
Cell & Tissue Engineering
Junzhi Yi, Jiayan Zhang, Qin Zhang, Xuri Chen, Rujie Qi, Renjie Liang, Ying Wang, Fei Wang, Yuliang Zhong, Xianzhu Zhang, Grace Chin, Qi Liu, Wenyan Zhou, Hua Liu, Jiansong Chen, Hongwei Ouyang
Summary: In this study, immunoprivileged and pro-angiogenic cells (IACs) were generated from human adipose tissues using a chemically defined cocktail. Compared to conventional adipose-derived MSCs (cADSCs), IACs exhibited enhanced stemness capacity and improved safety. IACs further promoted nitric oxide production, anti-apoptotic ability, and tube formation of human vein endothelial cells through superior paracrine effects.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Article
Materials Science, Ceramics
Patricia Ros-Tarraga, Carlos M. Martinez, Miguel A. Rodriguez, Piedad N. De Aza
Summary: The elderly population is increasing, leading to an increase in bone and joint problems. Scaffold for bone tissue regeneration can solve these problems, and material selection is crucial for satisfactory results. This study designed a three-dimensional porous ceramic scaffold and analyzed its biocompatibility and osteoinductive capacity. The results suggest that it has potential applications as bone substitutes.
CERAMICS INTERNATIONAL
(2022)
Review
Biochemistry & Molecular Biology
Parisa Khayambashi, Janaki Iyer, Sangeeth Pillai, Akshaya Upadhyay, Yuli Zhang, Simon D. Tran
Summary: Tissue engineering has a long history in regenerative medicine, and methods such as cell-encapsulated hydrogels and exosomes can enhance the regenerative potential of various tissues, with hydrogels being the most user-friendly, economical, and accessible material for encapsulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Mian Wu, Lifeng Chen, Yuhan Qi, Hai Ci, Shan Mou, Jie Yang, Qiaoyu Yuan, Weiqi Yao, Zhenxing Wang, Jiaming Sun
Summary: Using umbilical cord mesenchymal stem cells (hUCMSCs) as seed cells for tissue-engineered breast construction has shown significant advantages, promoting adipose tissue regeneration and enhancing angiogenesis. The transplantation of hUCMSCs was found to increase fat tissue content, reduce macrophage numbers, and exhibit good differentiation capability in adipose tissue.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell Biology
Furkan Yigitbilek, Sabena M. Conley, Hui Tang, Ishran M. Saadiq, Kyra L. Jordan, Lilach O. Lerman, Timucin Taner
Summary: MSCs from liver and adipose tissue showed similar proliferation and migration properties, but had differences in immunomodulatory and pro-angiogenic abilities. Selecting cell-based therapy according to the specific characteristics of different cell sources may be important for treating certain diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Sabin J. Bozso, Jimmy J. H. Kang, Ryaan El-Andari, Nicholas Fialka, Lin Fu Zhu, Steven R. Meyer, Darren H. Freed, Jayan Nagendran, Jeevan Nagendran
Summary: Autologous mesenchymal stem cell recellularization of xenogenic valves reduces the xenoreactive immune response in an in vivo rat model and may be an effective approach to decrease the progression of xenograft valve dysfunction.
EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY
(2022)
Article
Pharmacology & Pharmacy
Prakash Gangadaran, Ramya Lakshmi Rajendran, Ji Min Oh, Eun Jung Oh, Chae Moon Hong, Ho Yun Chung, Jaetae Lee, Byeong-Cheol Ahn
Summary: The study found that extracellular vesicles (EVs) derived from stem cells isolated from human adipose tissue contain angiogenic proteins and promote angiogenesis in vitro and in vivo.
Article
Biochemistry & Molecular Biology
Nadia Nascimento da Rosa, Julia Maurer Appel, Ana Carolina Irioda, Bassam Felipe Mogharbel, Nathalia Barth de Oliveira, Maiara Carolina Perussolo, Priscila Elias Ferreira Stricker, Livia Rosa-Fernandes, Claudio Romero Farias Marinho, Katherine Athayde Teixeira de Carvalho
Summary: A 3D lung model was developed using Wharton's jelly mesenchymal stem cells (WJ-MSC) for pulmonary differentiation and bioprinting. The differentiation of WJ-MSC into lung cells and bioprinting in a 3D structure were successfully achieved, providing a promising alternative for in vitro drug testing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Silvia Pisani, Stefania Croce, Simone Mauramati, Marta Marmonti, Lorenzo Cobianchi, Irene Herman, Rossella Dorati, Maria Antonietta Avanzini, Ida Genta, Marco Benazzo, Bice Conti
Summary: The use of a polymeric full-thickness scaffold engineered with mesenchymal stem cells (MSCs) shows promise in promoting esophageal regeneration and avoiding the need for autologous conduits.
Article
Biochemistry & Molecular Biology
Baoqiang Guo, Xenia Sawkulycz, Nima Heidari, Ralph Rogers, Donghui Liu, Mark Slevin
Summary: The study of MFAT demonstrates that micro-fragmented adipose tissue from different samples can induce the formation of mesenchymal stem cells. The secretions of cytokines from MFAT samples have effects including anti-inflammatory, pro-regeneration, anti-scarring, pro-angiogenesis, and anti-bacterial properties. MFAT in different samples show varying effects on angiogenesis and inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Sebastian Blatt, Daniel G. E. Thiem, Andreas Pabst, Bilal Al-Nawas, Peer W. Kaemmerer
Summary: The combination of bone substitute materials (BSMs) with platelet-rich fibrin (PRF) showed significant positive pro-angiogenic effects, particularly with alloplastic and xenogeneic materials, leading to increased vessel formation in vivo. This demonstrates the enhanced angiogenetic potential of BSMs when combined with PRF, suggesting potential for bony regeneration in clinical applications.
Article
Biochemistry & Molecular Biology
Agnieszka Krawczenko, Aleksandra Klimczak
Summary: MSCs, known for their regenerative and immunomodulatory properties, are typically isolated from bone marrow and adipose tissue. This review focuses on MSCs derived from adipose tissue and their secretome in regeneration processes, with a special emphasis on their contribution to angiogenic processes. Various clinical trials have been registered to explore the application of AT-MSCs and their secretome in treating different medical conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Veronika Smolinska, Michaela Debreova, Martina Culenova, Maria Csobonyeiova, Andrey Svec, Lubos Danisovic
Summary: Healing of articular cartilage defects is challenging and traditional reparative approaches are limited. Mesenchymal stromal cells have been extensively studied and proven to have therapeutic effects on articular cartilage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Biochemistry & Molecular Biology
Roberto Benelli, Maria Raffaella Zocchi, Alessandro Poggi
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Francesca Tosetti, Massimo Alessio, Alessandro Poggi, Maria Raffaella Zocchi
Summary: Enzymes are no longer considered static molecular machines, as they can move to different locations and change their functions. Recent research focuses on the regulation and function of ADAM endopeptidases at specific subcellular sites or in multimolecular complexes, particularly ADAM10 and TACE/ADAM17.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Massimo Alfano, Irene Locatelli, Cristina D'Arrigo, Marco Mora, Giovanni Vozzi, Aurora De Acutis, Roberta Pece, Sara Tavella, Delfina Costa, Alessandro Poggi, Maria Raffaella Zocchi
Summary: This study reveals that increased stiffness and altered architecture of the extracellular matrix (ECM) are detectable in human lymphomas, and these modifications are related to the type and grading of the lymphoma. The findings suggest that digital pathology may assist in the diagnosis and treatment of lymphomas.
Review
Oncology
Alessandro Poggi, Maria Raffaella Zocchi
Summary: The discovery of immune checkpoints and the development of specific blockers have revolutionized anti-cancer therapy. This review focuses on the potential of NK ICs as targets for dual-checkpoint inhibition, highlighting their role in NK cell survival and anti-tumor immune response.
MOLECULAR THERAPY-ONCOLYTICS
(2022)
Article
Cell Biology
Makram Merimi, Hassan Fahmi, Joery De Kock, Charline Beguin, Arsene Burny, Guido Moll, Alessandro Poggi, Mehdi Najar
Article
Cell Biology
Federico Ferro, Renza Spelat, Georgina Shaw, Cynthia M. Coleman, Xi Zhe Chen, David Connolly, Elisabetta M. F. Palama, Chiara Gentili, Paolo Contessotto, Mary J. Murphy
Summary: This study evaluated the anti-inflammatory and regenerative effects of bone marrow MSC and their extracellular vesicles in a diabetic fractured mouse model. The results showed that preconditioned cells promoted earlier soft callus formation and mineralization at the fracture site, suggesting the importance of preconditioning in MSC therapy research and optimization.
Article
Oncology
Roberto Benelli, Delfina Costa, Laura Salvini, Samuele Tardito, Francesca Tosetti, Federico Villa, Maria Raffaella Zocchi, Alessandro Poggi
Summary: This study generated a novel ADC called Cet-ZA, which can specifically target CRC. Importantly, patient-derived CRC organoids or CRC tumor cell suspensions could induce the expansion of Vδ2 T cells from peripheral blood and tumor infiltrating lymphocytes when primed with Cet-ZA, and Cet-ZA can promote Vδ2 T cell-mediated killing of CRC organoids.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Editorial Material
Oncology
Serena Matis, Alessandro Poggi, Roberto Benelli
Editorial Material
Oncology
Roberto Benelli, Maria Raffaella Zocchi, Alessandro Poggi
Editorial Material
Oncology
Alessandro Poggi, Serena Matis, Roberto Benelli
Editorial Material
Oncology
Roberto Benelli, Maria Raffaella Zocchi, Alessandro Poggi
Editorial Material
Cell Biology
Alessandro Poggi
Article
Cell Biology
Delfina Costa, Roberta Vene, Simona Coco, Luca Longo, Francesca Tosetti, Stefano Scabini, Luca Mastracci, Federica Grillo, Alessandro Poggi, Roberto Benelli
Summary: The p38 inhibitor SB202190 is an essential component of normal colorectal mucosa cultures. It has been found that this molecule can stabilize EGFR signaling and promote organoid proliferation by increasing phosphorylation of Erk1-2. However, the growth of some colorectal cancer-derived organoid cultures is inhibited by SB202190 through an unknown mechanism.
Article
Cell Biology
Carlo Genova, Roberta Tasso, Alessandra Rosa, Giovanni Rossi, Daniele Reverberi, Vincenzo Fontana, Silvia Marconi, Michela Croce, Maria Giovanna Dal Bello, Chiara Dellepiane, Marco Tagliamento, Maria Chiara Ciferri, Lodovica Zullo, Alessandro Fedeli, Angela Alama, Katia Cortese, Chiara Gentili, Eugenia Cella, Giorgia Anselmi, Marco Mora, Giulia Barletta, Erika Rijavec, Francesco Grossi, Paolo Pronzato, Simona Coco
Summary: The advent of immune checkpoint inhibitors (ICIs) has dramatically changed the treatment of non-small cell lung cancer (NSCLC). However, their efficacy remains limited to a subset of patients. This study evaluated the prognostic role of soluble (s) immune checkpoints and immune checkpoints associated with extracellular vesicles (EVs) in NSCLC patients treated with ICIs. The results showed that high levels of sPD-L1, sB7-H3, and sB7-H4 were associated with different prognoses in advanced NSCLC patients treated with ICIs. The study also suggested potential involvement of EVs in the disease response to ICIs.
Article
Dermatology
Giulia Gasparini, Roberta Tasso, Maria Elisabetta Federica Palama, Maria Chiara Ciferri, Chiara Gentili, Giovanni Di Zenzo, Alessia Provini, Adele Salemme, Rodolfo Quarto, Aurora Parodi, Emanuele Cozzani
Summary: This study aimed to investigate the presence of BP targeted autoantigens in blister fluid-derived extracellular vesicles (EVs). EVs were isolated from blister fluid of BP patients and suction blister fluid of healthy donors, and EV characterization was performed. Western blot analysis revealed the presence of BP180 in EVs derived from blister fluid of 3 out of 6 BP patients, while no BP180 was detected in EVs from healthy donors. This discovery provides insights into the pathogenesis of BP.
ARCHIVES OF DERMATOLOGICAL RESEARCH
(2023)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.