Review
Cell Biology
Yuan Liu, Christina Holmes
Summary: This review explores the tissue regeneration potential of EVs isolated from bone marrow and adipose tissue, the two most common clinical sources of adult MSCs, focusing on their angiogenic, osteogenic, and immunomodulatory potentials. Other important issues in the development of MSC-derived EV based therapies are also discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Immunology
Zixin Zhou, Yan Tao, Hui Zhao, Qun Wang
Summary: Macrophages play critical roles in regulating immunometabolic homeostasis in adipose tissue by cooperating with adipocytes, ADSCs, and other cells through extracellular vesicles (EVs). Adipose EVs actively participate in the interactions between macrophages, adipocytes, and ADSCs under healthy or obese conditions, influencing immunometabolism significantly.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell & Tissue Engineering
Chuan He, Minjia Dai, Xiaojie Zhou, Jie Long, Weidong Tian, Mei Yu
Summary: Our study compared adipose tissue extract (ATE) and its further purified small extracellular vesicles (sEV-AT) in terms of characteristics, inductivity on cells, and capacity for promoting de novo adipose tissue regeneration. sEV-AT showed higher particle yield and concentrated particle size distribution, while ATE had more soluble factors and multiple peaks in particle size distribution.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell Biology
Andrea L. Estrada, Zackary J. Valenti, Gabriella Hehn, Adam J. Amorese, Nicholas S. Williams, Nicholas P. Balestrieri, Clayton Deighan, Christopher P. Allen, Espen E. Spangenburg, Nicole A. Kruh-Garcia, Daniel S. Lark
Summary: This study found that skeletal muscle tissue secretes more extracellular vesicles (EVs) compared to white adipose tissue (WAT), and a significant proportion of EVs from skeletal muscle tissue are derived from myofibers. Furthermore, it was shown that these myofiber-derived EVs can reach the circulation in vivo.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Engineering, Biomedical
Peng-Cheng Liu, Qiu-Wen Tan, Yi Zhang, Hua Wang, Li Zhou, Qian-Ru Yang, Li Xu, Tao He, Hui-Qi Xie, Qing Lv
Summary: The study found that HAPA had a positive impact on ADSCs proliferation, VEGF secretion, and adipogenic differentiation in vitro, and demonstrated improved volume retention and angiogenesis, as well as reduced cell apoptosis in in vivo study compared to ADSCs-assisted lipofilling and pure lipofilling.
BIOMEDICAL MATERIALS
(2021)
Review
Biochemistry & Molecular Biology
Mariachiara Zuccarini, Patricia Giuliani, Valentina Di Liberto, Monica Frinchi, Francesco Caciagli, Vanni Caruso, Renata Ciccarelli, Giuseppa Mudo, Patrizia Di Iorio
Summary: In the past decade, several compounds have been identified for treating obesity, but concerns about their efficacy and safety remain due to the complexity of the disease. Therefore, it is crucial to discover new factors involved in obesity induction and progression. Adipose stromal/stem cells release extracellular vesicles (EVs), which play an important role in regulating metabolic processes. However, there are advantages and disadvantages to using EVs as potential next-generation anti-obesity agents.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Furkan Yigitbilek, Sabena M. Conley, Hui Tang, Ishran M. Saadiq, Kyra L. Jordan, Lilach O. Lerman, Timucin Taner
Summary: MSCs from liver and adipose tissue showed similar proliferation and migration properties, but had differences in immunomodulatory and pro-angiogenic abilities. Selecting cell-based therapy according to the specific characteristics of different cell sources may be important for treating certain diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Byong Seung Cho, Sung-Bae Kim, Sokho Kim, Beomseok Rhee, Jungho Yoon, Jae Won Lee
Summary: In this study, the effects of canine ASC-exosomes on canine AD were investigated using a Biostir-induced AD mouse model. The study found that cASC-EVs improved AD-like dermatitis and decreased serum IgE, ear thickness, and inflammatory cytokines such as IL-4 and IFN-γ in a dose-dependent manner. In vivo toxicity studies showed no systemic toxicity. miRNA analysis showed the presence of anti-inflammatory miRNAs in cASC-EVs, suggesting a promising cell-free therapy for treating canine AD.
Article
Cell Biology
Hong Gao, Zhenlong Luo, Zhongmou Jin, Yudong Ji, Wei Ying
Summary: The study revealed that ATM EVs from obese mice play a significant role in inhibiting insulin secretion and promoting beta cell proliferation, with miR-155 identified as a highly enriched miRNA in obese ATM EVs and exerting critical regulatory effects on beta cell responses.
Article
Emergency Medicine
Ye Li, Xin Bi, Mengfan Wu, Xinyao Chen, Weiqing Zhan, Ziqing Dong, Feng Lu
Summary: This study aimed to physically alter the stiffness of decellularized adipose tissue (DAT) to improve adipose regeneration. Three different cell-free hydrogel systems were formed by physically cross-linking DAT with different concentrations of methyl cellulose (MC). The results demonstrated that adjusting the stiffness of DAT via physical cross-linking with MC can effectively promote adipose regeneration.
Article
Materials Science, Multidisciplinary
Yongjin Sun, Juntao Zhang, Bi Chen, Yunlong Yang, Haiyan Li, Xin Niu, Qing Li, Weidong Wu, Zongping Xie, Yunfeng Chen, Fuyue Wu, Yang Wang
Summary: This study demonstrates the effectiveness of USC-sEVs in accelerating wound healing in aged mice by ameliorating cellular senescence and reducing SASP secretion. USC-sEVs loaded in HAAM can effectively accelerate wound healing by controlling release and locating the sEVs in the wound site.
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
(2021)
Article
Medicine, Research & Experimental
Grace Huang, Zhongjian Cheng, Alycia Hildebrand, Chunlin Wang, Maria Cimini, Rakija Roy, Anna Maria Lucchese, Cindy Benedict, Vandana Mallaredy, Ajit Magadum, Darukeshwara Joladarashi, Charan Thej, Carolina Gonzalez, May Trungcao, Venkata Naga Srikanth Garikipati, John W. Elrod, Walter J. Koch, Raj Kishore
Summary: This study suggests that diabetes impairs the function of EPC-EVs in repairing ischemic heart damage through changes in histone modification and acetylation. The use of histone deacetylase (HDAC) inhibitors may potentially restore the function of EPC and other stem cells for autologous cell therapy applications.
Review
Nanoscience & Nanotechnology
Yu-Guo Yuan, Jia-Lin Wang, Ya-Xin Zhang, Ling Li, Abu Musa Md Talimur Reza, Sangiliyandi Gurunathan
Summary: This review discusses the potential function of mesenchymal stem cell-derived exosomes (MSCs-EXOs) in various diseases including neurological, cancer, autoimmune, and inflammatory diseases. It also highlights the potential use of MSCs-EXOs in treating COVID-19 inflammatory disease. Additionally, it provides an overview of exosome biogenesis, factors regulating it, strategies, and challenges of using MSCs-EXOs.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Siti Syahira Airuddin, Ahmad Sukari Halim, Wan Azman Wan Sulaiman, Ramlah Kadir, Nur Azida Mohd Nasir
Summary: Stem cells, particularly adipose-derived stem cells, have shown promising potential in disease treatment due to their differentiation abilities and secretion of growth factors. However, more research is needed to fully understand the safety and effectiveness of adipose-derived stem cells in medical treatment.
Article
Endocrinology & Metabolism
Clair Crewe, Philipp E. Scherer
Summary: Dysfunction of functional adipose tissue contributes to pathological metabolism and disease development, while adipocyte-derived EVs play a crucial role in regulating metabolism and have the potential for treating obesity-related diseases.
REVIEWS IN ENDOCRINE & METABOLIC DISORDERS
(2022)
Article
Oncology
Ji-hoon Jung, Chae-Moon Hong, Il Jo, Shin-Young Jeong, Sang-Woo Lee, Jaetae Lee, Byeong-Cheol Ahn
Summary: This study aimed to evaluate whether serum alkaline phosphatase (ALP) can differentiate flare phenomenon from disease progression on bone scintigraphy in breast or prostate cancer patients with bone metastasis. The results suggest that ALP is a useful serologic marker to differentiate flare phenomenon from disease progression on bone scintigraphy in patients with bone metastasis.
Article
Biochemistry & Molecular Biology
Joon Seok Lee, Gyeong Hwa Kim, Jong Ho Lee, Jeong Yeop Ryu, Eun Jung Oh, Hyun Mi Kim, Suin Kwak, Keun Hur, Ho Yun Chung
Summary: In this study, miR-365a/b-3p was identified as a potential biomarker for hypertrophic scarring, suggesting its contribution to the formation of hypertrophic scars.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Prakash Gangadaran, Eun Jung Oh, Ramya Lakshmi Rajendran, Hyun Mi Kim, Ji Min Oh, Suin Kwak, Chae Moon Hong, Kang Young Choi, Ho Yun Chung, Byeong-Cheol Ahn
Summary: This study explores the use of human normal fibroblast-derived extracellular vesicles (hNF-EVs) for wound healing. The researchers found that hNF-EVs contain proangiogenic factors and can promote the proliferation and migration of fibroblasts and endothelial cells, as well as accelerate wound healing in mice. The delivery of hNF-EVs without the need for scaffolds and invasiveness offers a novel approach for efficient wound healing.
Review
Chemistry, Medicinal
Gollahalli Shivashankar Prajwal, Naveen Jeyaraman, Krishna Kanth, Madhan Jeyaraman, Sathish Muthu, Sree Naga Sowndary Rajendran, Ramya Lakshmi Rajendran, Manish Khanna, Eun Jung Oh, Kang Young Choi, Ho Yun Chung, Byeong-Cheol Ahn, Prakash Gangadaran
Summary: Tissue engineering and regenerative medicine provide a new approach for treating musculoskeletal diseases, utilizing MSCs and growth factors to improve the regenerative environment. However, the selection of MSCs source and the long-term and short-term effects of MSCs treatment remain uncertain.
Article
Biology
Luciano C. Ramires, Madhan Jeyaraman, Sathish Muthu, Navaladi A. Shankar, Gabriel Silva Santos, Lucas Furtado da Fonseca, Jose Fabio Lana, Ramya Lakshmi Rajendran, Prakash Gangadaran, Manasi P. Jogalekar, Alfredo A. Cardoso, Alex Eickhoff
Summary: Orthobiologics, including various cell populations, cytokines, and growth factors, show potential in promoting tissue regeneration and homeostasis in Achilles tendinopathy, a condition characterized by disrupted healing responses, persistent inflammation, and catabolic reactions. Further studies and clinical trials are needed to validate the clinical utility and efficacy of these therapeutic strategies in the management of tendinopathies.
Article
Biochemistry & Molecular Biology
Prakash Gangadaran, Gowri Rangaswamy Gunassekaran, Ramya Lakshmi Rajendran, Ji Min Oh, Sri Murugan Poongkavithai Vadevoo, Ho Won Lee, Chae Moon Hong, Byungheon Lee, Jaetae Lee, Byeong-Cheol Ahn
Summary: This study proposes the use of a targeting peptide, IL4RPep-1, to specifically deliver extracellular vesicles (EVs) to thyroid tumors. In vivo experiments confirmed the accumulation of IL4RPep-1-EVs in the tumor, while the control group showed no accumulation.
Review
Biotechnology & Applied Microbiology
Ragunanthan Narayanaswamy, Bishnu Prasad Patro, Naveen Jeyaraman, Prakash Gangadaran, Ramya Lakshmi Rajendran, Arulkumar Nallakumarasamy, Madhan Jeyaraman, Prasanna Ramani, Byeong-Cheol Ahn
Summary: Various forms of platelet concentrates have evolved over the past few decades, with newer generation products such as leukocyte-platelet-rich fibrin (L-PRF) and advanced platelet-rich fibrin (A-PRF) showing superior biological properties in musculoskeletal regeneration compared to the first-generation concentrates. These newer platelet concentrates have a complete matrix of physiological fibrin and facilitate intercellular signaling and migration, promoting angiogenic, chondrogenic, and osteogenic activities. A-PRF, with higher leukocyte inclusion, also possesses antimicrobial activity and can replicate various physiological and immunological factors of wound healing. Its applications have expanded to include the management of musculoskeletal conditions, reconstructive cosmetic surgery, wound healing in diabetic patients, and maxillofacial surgeries.
BIOENGINEERING-BASEL
(2023)
Review
Biotechnology & Applied Microbiology
Bushu Harna, Vijay Gupta, Shivali Arya, Naveen Jeyaraman, Ramya Lakshmi Rajendran, Madhan Jeyaraman, Prakash Gangadaran, Manish Khanna, Chae Moon Hong, Byeong-Cheol Ahn
Summary: Adhesive capsulitis shoulder is a common problem, and this study evaluated the efficacy of intra-articular injections of platelet-rich plasma (PRP) in the treatment. The results showed that PRP injections improved clinical and functional outcomes in patients with adhesive capsulitis shoulder, and no major side effects were observed.
BIOENGINEERING-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Prakash Gangadaran, Eun Jung Oh, Ramya Lakshmi Rajendran, Ji Min Oh, Hyun Mi Kim, Suin Kwak, Ho Yun Chung, Jaetae Lee, Byeong-Cheol Ahn, Chae Moon Hong
Summary: Cultured MSCs exhibit different effects on their secretomes depending on whether they are grown as 2D monolayers or 3D spheroids. The secretomes of 3D-MSCs contain higher levels of proangiogenic factors compared to 2D-MCs. The secretomes of 3D-MSCs promote proliferation, migration, and tube formation of ECs, and show enhanced wound healing in a burn injury mouse model.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Review
Multidisciplinary Sciences
Madhan Jeyaraman, Ramya Lakshmi Rajendran, Sathish Muthu, Naveen Jeyaraman, Shilpa Sharma, Saurabh Kumar Jha, Purushothaman Muthukanagaraj, Chae Moon Hong, Lucas Furtado da Fonseca, Jose Fabio Santos Duarte Lana, Byeong-Cheol Ahn, Prakash Gangadaran
Summary: Globally, neurological diseases, particularly Alzheimer's disease, have a significant impact on healthcare professionals. Alzheimer's disease is a progressive neurological disorder that leads to the loss of neurons and synapses in the brain. The treatment of Alzheimer's disease remains controversial, and this paper examines the potential use of genetically modified stem cells and extracellular vesicles in regenerative medicine for Alzheimer's disease.
Article
Radiology, Nuclear Medicine & Medical Imaging
Junik Son, Chae Moon Hong, Do-Hoon Kim, Shin Young Jeong, Sang-Woo Lee, Jaetae Lee, Byeong-Cheol Ahn
Summary: This study investigates the prognostic value of response to therapy (RTT) assessment using stimulated-thyroglobulin (sti-Tg) and nonstimulated-thyroglobulin (nonsti-Tg) in differentiated thyroid cancer (DTC) patients. Results show that sti-Tg assessment is a better predictor for recurrence-free survival (RFS) compared to nonsti-Tg assessment. However, among patients classified as excellent response (ER) on initial sti-Tg assessment, a second sti-Tg assessment may not be necessary if classified as ER on the second nonsti-Tg assessment.
NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2023)
