Article
Cell & Tissue Engineering
Gang Wang, Fengbin Wang, Lecheng Zhang, Chao Yan, Yuelei Zhang
Summary: miR-133a is strongly associated with GIO and similar disorders induced by glucocorticoids in MSCs. Silencing miR-133a resulted in positive effects on GC-treated MSCs and on bone loss in GIO animal models. Moreover, the FGFR1-MAPK/ERK signaling may be involved in the protective effect of miR-133a silencing.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Engineering, Biomedical
Laila A. Damiati, Monica P. Tsimbouri, Virginia-Llopis Hernandez, Vineetha Jayawarna, Mark Ginty, Peter Childs, Yinbo Xiao, Karl Burgess, Julia Wells, Mark R. Sprott, R. M. Dominic Meek, Peifeng Li, Richard O. C. Oreffo, Angela Nobbs, Gordon Ramage, Bo Su, Manuel Salmeron-Sanchez, Matthew J. Dalby
Summary: This study developed a polymer coating with nano-topographical structure to support osteospecific stem cells and prevent bacterial adhesion and biofilm formation. The experimental results showed that this coating can increase cell adhesion and osteoblast differentiation, and reduce biofilm formation.
Article
Engineering, Biomedical
Liaobing Xin, Xiaowen Zheng, Jianmin Chen, Sentao Hu, Yilun Luo, Qunzi Ge, Xiaoying Jin, Lie Ma, Songying Zhang
Summary: This study explores an acellular biomaterial called SDF-1 alpha/E7-modified collagen scaffold (CES), which enables near-complete endometrium regeneration and fertility restoration by mobilizing endogenous MSCs. The results demonstrate that CES can overcome the traditional limitations of cell-based therapies and serve as a clinically feasible and cell-free strategy with high therapeutic efficiency for IUA treatment.
ADVANCED HEALTHCARE MATERIALS
(2022)
Review
Polymer Science
Aiah A. El-Rashidy, Sara El Moshy, Israa Ahmed Radwan, Dina Rady, Marwa M. S. Abbass, Christof E. Doerfer, Karim M. Fawzy El-Sayed
Summary: The stiffness of the extracellular matrix plays a regulatory role in directing the osteogenic differentiation of MSCs, with MSCs sensing and responding to their ECM to undergo differentiation. Customizing scaffold materials to mimic cells' natural environment is crucial in the tissue engineering process.
Review
Immunology
Pan Luo, Qiling Yuan, Xianjie Wan, Mingyi Yang, Peng Xu
Summary: The pathological manifestations of osteoarthritis (OA) involve destruction of articular cartilage, synovial sac thickening, subchondral osteosclerosis, and osteophyte formation. Various cell types, including chondrocytes, osteoblasts, osteoclasts, synovial fibroblasts, T cells, macrophages, and mesenchymal stem cells (MSCs), are involved in OA. Immune cells, especially dendritic cells and macrophages, play significant roles in the pathogenesis of OA. Additionally, adaptive immune cells such as T-cell subsets, B-cell subsets, and NK cells are important in OA pathogenesis. MSCs not only play a key role in maintaining the dynamic balance and repairing OA tissue but also have immunomodulatory properties. Therefore, MSCs have potential as a therapeutic agent for OA. This review summarizes the effects of immune cells and cytokines on different cells in OA, aiming to identify new therapeutic targets based on the immune system and cytokine activity sites to prevent OA development.
JOURNAL OF INFLAMMATION RESEARCH
(2023)
Article
Cell & Tissue Engineering
Jiayue Huang, Wenwen Zhang, Jie Yu, Yating Gou, Nizhou Liu, Tingting Wang, Congcong Sun, Benyuan Wu, Changjiang Li, Xinpei Chen, Yanhua Mao, Yingfeng Zhang, Jia Wang
Summary: This study investigated the therapeutic effect of hAMSCs combined with PPCNg transplantation on promoting the regeneration of injured endometrium. The results showed that hAMSCs maintained normal cell proliferation, apoptosis and cell cycle in PPCNg. In addition, hAMSCs combined with PPCNg intrauterine transplantation facilitated the regeneration of injured endometrium and restored reproductive capacity.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell & Tissue Engineering
Luca Dalle Carbonare, Jessica Bertacco, Giulia Marchetto, Samuele Cheri, Michela Deiana, Arianna Minoia, Natascia Tiso, Monica Mottes, Maria Teresa Valenti
Summary: The study investigated the effects of MSM on chondrogenesis and osteogenesis, showing that MSM promotes chondrogenesis and preosteoblast formation. It suggests that MSM could be a useful tool to counteract skeletal degenerative diseases.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Oncology
Thi My Hang Nguyen, Yi-Shyun Lai, Ying-Chi Chen, Tzu-Chien Lin, Ngoc Thang Nguyen, Wen-Tai Chiu
Summary: This study found that under hypoxic conditions, the transcription factor YAP is activated in mesenchymal triple-negative breast cancer (TNBC) cells, promoting cell migration. In addition, hypoxia causes the accumulation of focal adhesions (FAs) at the leading edge of tumor cells. However, the use of the YAP inhibitor verteporfin significantly reduces cell migration and prevents the accumulation of FAs under hypoxic conditions, but only in mesenchymal TNBC cells.
Article
Engineering, Biomedical
Liaobing Xin, Cheng Wei, Xiaomei Tong, Yangyang Dai, Dong Huang, Jianmin Chen, Lie Ma, Songying Zhang
Summary: Stem cell-derived apoptotic bodies (ABs) have been shown to promote endometrial regeneration through immunomodulation, cell proliferation, and angiogenesis. Incorporating ABs into hyaluronic acid hydrogel can effectively reduce fibrosis and promote endometrial regeneration in mouse and rat models, leading to fertility restoration.
BIOACTIVE MATERIALS
(2022)
Article
Cell & Tissue Engineering
Yi Liu, Jingan Chen, Haowei Liang, Yueqin Cai, Xinyue Li, Li Yan, Li Zhou, Letian Shan, Hui Wang
Summary: The study found that hucMSCs can repair vascular endothelial damage in diabetic patients and exert their effects through a paracrine action mechanism mediated by the MAPK/ERK signaling pathway. This research provides new insights into the treatment of diabetes and suggests a potential strategy for the clinical treatment of diabetic vascular complications.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Engineering, Biomedical
Ho-Ying Wan, Rita Lih Ying Shin, Jack Chun Hin Chen, Marisa Assuncao, Dan Wang, Susie K. Nilsson, Rocky S. Tuan, Anna Blocki
Summary: The study demonstrated that supplementation of Dextran sulfate (DxS) in osteogenically induced cultures of human mesenchymal stem cells (MSCs) amplified the deposition of osteoblastic extracellular matrix (ECM). This enhancement led to increased collagen type I deposition and osteogenic gene expression, as well as enrichment in bone morphogenetic protein 2 (BMP-2), suggesting a potential role of DxS in promoting osteogenic differentiation by MSCs.
ACTA BIOMATERIALIA
(2022)
Article
Chemistry, Multidisciplinary
Bini Zhou, Bo Yang, Qian Liu, Lu Jin, Yu Shao, Taoyang Yuan, Ya-nan Zhang, Chao Wang, Ziwei Shi, Xin Li, Yufan Pan, Ning Qiao, Jiang-Fei Xu, Yuhe Renee Yang, Yuanchen Dong, Lijin Xu, Songbai Gui, Dongsheng Liu
Summary: Mechanical interactions between cells and extracellular matrix (ECM) play a critical role in determining stem cell fate. Synthetic models of ECM, like hydrogels, can be used to manipulate the mechanical properties of the cell niche and investigate the effects of mechanical signals on cell behavior. However, it has been challenging to only change the mechanical signals of hydrogels without altering their chemical and topological properties.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Tong Gao, Xu Yang, Masayoshi Fujisawa, Toshiaki Ohara, Tianyi Wang, Nahoko Tomonobu, Masakiyo Sakaguchi, Teizo Yoshimura, Akihiro Matsukawa
Summary: The downregulation of SPRED2 promotes EMT and stemness in hepatocellular carcinoma through the activation of the ERK1/2 pathway, leading to more malignant phenotypes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Arseniy Lobov, Polina Kuchur, Aigul Khizhina, Anastasia Kotova, Andrei Ivashkin, Daria Kostina, Polina Klausen, Evgeniia Khokhlova, Egor Repkin, Kseniia Postnikova, Daria Perepletchikova, Evgeny Denisov, Tatiana Gerashchenko, Rashid Tikhilov, Svetlana Bozhkova, Andrey Sereda, Vitaliy Karelkin, Natella Enukashvily, Anna Malashicheva
Summary: In this study, we conducted a comparative analysis of mesenchymal cell primary cultures from various sources and found differences in their ability to undergo osteogenic differentiation and produce extracellular matrix. Based on the analysis of secretome, proteome, and transcriptome, we classified them into dental stem cells, mesenchymal stem cells, and fetal stem cells. Although they have differences, mesenchymal cells from all sources secreted core bone extracellular matrix-associated proteins. These findings shed light on the distinctive characteristics of mesenchymal stem cells and provide insights into their potential applications in regenerative medicine and the inherent diversity of mesenchymal cells in vivo.
Article
Engineering, Biomedical
Sadegh Ghorbani, Annette Christine Fuchtbauer, Andreas Mollebjerg, Pia Moller Martensen, Sara Hvidbjerg Laursen, David Christian Evar Kraft, Jorgen Kjems, Rikke Louise Meyer, Karim Rahimi, Morten Foss, Ernst-Martin Fuchtbauer, Duncan S. Sutherland
Summary: Biochemical and biomechanical signals in the niche environments regulate stem cell function. A study compared in vitro signals from LN-511 and/or topographic cues to maintain stemness in mESCs. The integration of LN-511 with nanotopography decreased colony growth but maintained stemness markers, suggesting the antagonistic effect of combining biochemical and topographic signals. Faster colony growth was observed at nanotopographies without LN-511, indicating improved ESC expansion.
Editorial Material
Endocrinology & Metabolism
Jillian Cornish, Richard O. C. Oreffo
Article
Orthopedics
Yavor Pukalski, Jan Barcik, Ivan Zderic, Parvan Yanev, Asen Baltov, Orlin Malouchev, Karl Stoffel, Geoff Richards, Boyko Gueorguiev, Dian Enchev
Summary: In this study, the fixation strength of coronoid replacement using an individualized 3D printed prosthesis with curved cemented intramedullary (IM) stem was compared to radial head grafted reconstruction and screw fixation. The results showed that the prosthesis group had significantly higher stiffness and failure load compared to the other groups, and the fixation group had significantly lower stiffness compared to the intact group. This method of coronoid process replacement seems to be an effective way to restore coronoid buttress function.
JOURNAL OF SHOULDER AND ELBOW SURGERY
(2023)
Article
Chemistry, Physical
Olga Guselnikova, Roman Elashnikov, Vaclav Svorcik, Martin Kartau, Cameron Gilroy, Nikolaj Gadegaard, Malcolm Kadodwala, Affar S. S. Karimullah, Oleksiy Lyutakov
Summary: The use of surface enhanced Raman scattering (SERS) combined with disposable chiral plasmonic shurikens and silver nanoclusters allows for effective detection and discrimination of chiral compounds with high sensitivity. Numerical simulations were used to study the effects of shuriken geometry, silver nanostructured layer parameters, and SERS excitation wavelength(s) on the observed SERS response. The designed structures were able to successfully discriminate cysteine enantiomers and probe biomolecular chirality using a standard Raman spectrometer.
NANOSCALE HORIZONS
(2023)
Review
Oncology
Franziska Lioba Breulmann, Luan Phelipe Hatt, Boris Schmitz, Esther Wehrle, Robert Geoff Richards, Elena Della Bella, Martin James Stoddart
Summary: This systematic review aimed to identify common miRNAs involved in fracture healing or non-union fractures. Out of 151 miRNAs, miR-21, miR-140 and miR-214 were the most investigated miRNAs in fracture healing in general, while miR-31-5p, miR-221 and miR-451-5p were identified to be regulated specifically in non-union fractures. Despite heterogeneity between studies, these miRNAs have the potential to serve as biomarkers for non-union fractures.
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Article
Materials Science, Biomaterials
Antonio Conde-Gonzalez, Michael Glinka, Deepanjalee Dutta, Robert Wallace, Anthony Callanan, Richard O. C. Oreffo, Mark Bradley
Summary: Regenerative medicine demands sophisticated 3D biomaterials to promote tissue formation. This study presents a novel technique for screening potential biocompatible 3D scaffolds, identifying a porous acrylate-based scaffold that promotes cell proliferation and tissue formation. The scaffold was fabricated through freeze-casting and photo-polymerization techniques and demonstrated tunable properties suitable for skeletal repair. This platform offers an innovative approach to scanning synthetic biomaterials for tissue regeneration.
BIOMATERIALS ADVANCES
(2023)
Article
Multidisciplinary Sciences
Slobodan Tepic, Daniel Arens, Tim Buchholz, Dirk Nehrbass, Olivera Cvetkovic, Martin J. Stoddart, R. G. Richards, Stephan Zeiter
Summary: Pneumonia, especially during the COVID-19 pandemic, has become a major public health and economic disaster. This study suggests that lung metabolism in inflammation may have common features regardless of the underlying cause. By comparing mice with bleomycin-induced lung inflammation to control groups, it was found that certain amino acids had significantly lower concentrations in the arterial blood, indicating that arginine replacement therapy may be beneficial in pneumonia.
Article
Chemistry, Multidisciplinary
Roxanna S. Ramnarine-Sanchez, Janos M. Kanczler, Nicholas D. Evans, Richard O. C. Oreffo, Jonathan I. Dawson
Summary: This paper reports a new reaction-diffusion self-assembly system, in which proteins function as diffusive reactants to assemble stable clay-protein composite hydrogels. Using this method, scalable and highly stable 3D protein patterns can be generated at sub-cellular resolution, and can guide cell behavior to achieve precise bone tissue template formation.
ADVANCED MATERIALS
(2023)
Article
Engineering, Biomedical
Till Berk, Ivan Zderic, Peter Schwarzenberg, Roman Pfeifer, Tatjana Pastor, Sascha Halvachizadeh, R. Geoff Richards, Boyko Gueorguiev, Hans-Christoph Pape
Summary: This biomechanical study concludes that minimally invasive cup fixation with screw augmentation demonstrates comparable stability to plate osteosynthesis for anterior column fractures in osteoporotic bone. However, the study does not provide a conclusive answer regarding the immediate full weight bearing following cup fixation. Therefore, a more conservative approach is considered more reasonable.
CLINICAL BIOMECHANICS
(2023)
Article
Biochemical Research Methods
Duanyang Xu, Konstantinos N. Bourdakos, Anna Crisford, Peter Johnson, Ibrahim Abughazaleh, Panuwat Srisamran, Richard O. C. Oreffo, Sumeet Mahajan, David J. Richardson, Lin Xu
Summary: We present an all-fiber ized 1840-nm thulium-fiber-laser source for label-free biological imaging through nonlinear microscopy. The source consists of a dissipative-soliton mode-locked seed laser and a chirped-pulse-amplification system. It utilizes short pulses generated by the mode-locked laser and pulse compression techniques to achieve high-quality imaging of biological samples.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Engineering, Biomedical
Riccardo Tognato, Romedi Parolini, Shahrbanoo Jahangir, Junxuan Ma, Sammy Florczak, R. Geoff Richards, Riccardo Levato, Mauro Alini, Tiziano Serra
Summary: In this study, a Faraday waves-based technique is demonstrated for the rapid assembly of osteoinductive β-Tricalcium phosphate (β-TCP) particles and human osteoblast pre-assembled in spheroids. The hydro-dynamic forces at the 'seabed' of the assembly chamber are utilized to tightly aggregate inorganic and biological entities at packing densities resembling native tissues. Additionally, by employing a layer-by-layer assembly procedure, centimeter scaled osteoinductive three-dimensional and cellularized constructs are fabricated for the first time using Faraday waves.
MATERIALS TODAY BIO
(2023)
Article
Medicine, General & Internal
Till Berk, Ivan Zderic, Peter Schwarzenberg, Ludmil Drenchev, Hristo Kostov Skulev, Roman Pfeifer, Tatjana Pastor, Geoff Richards, Boyko Gueorguiev, Hans-Christoph Pape
Summary: This study evaluated the biomechanical competence of posterior column acetabulum fracture fixation using either a standard cannulated screw or a cannulated compression headless screw (CCHS) via a posterior approach. The results showed that CCHS fixation had higher initial stiffness and fatigue performance compared to the standard cannulated screw. Therefore, CCHS fixation could be a valuable alternative option for treating posterior column acetabulum fractures.
MEDICINA-LITHUANIA
(2023)
Article
Medicine, General & Internal
Till Berk, Ivan Zderic, Peter Schwarzenberg, Torsten Pastor, Sascha Halvachizadeh, Geoff Richards, Boyko Gueorguiev, Hans-Christoph Pape, Umile Giuseppe Longo, Vicenzo Denaro, Edgaras Stankevicius
Summary: This study evaluated the biomechanical competence of S1-S2 sacroiliac (SI) screw fixation with and without using a washer across three different screw designs. The results showed that the number of cycles to failure and the corresponding load at failure were significantly higher for fully threaded short screws with a washer compared to its counterpart without a washer. However, there were no significant differences in these parameters when comparing fixations with and without a washer using either partially threaded short or fully threaded long transsacral screws.
MEDICINA-LITHUANIA
(2023)
Article
Materials Science, Biomaterials
Christina N. M. Ryan, Eugenia Pugliese, Naledi Shologu, Diana Gaspar, Peadar Rooney, Md Nahidul Islam, Alan O'Riordan, Manus J. Biggs, Matthew D. Griffin, Dimitrios I. Zeugolis
Summary: This study evaluated the effects of bidirectional surface topography, substrate rigidity, collagen type I coating, and macromolecular crowding on human bone marrow stem cell cultures. The results showed that surface topography and macromolecular crowding had significant influences on cell morphology and extracellular matrix deposition, respectively. Collagen type I coating increased cell metabolic activity. This study highlights the importance of multifactorial bioengineering in controlling cell fate.
BIOMATERIALS ADVANCES
(2023)
Article
Biochemistry & Molecular Biology
Enrico Bagnoli, Alexandre Trotier, Jill McMahon, Leo R. Quinlan, Manus Biggs, Abhay Pandit, Una Fitzgerald
Summary: Parkinson's disease is a progressive neurodegenerative disorder with unknown etiology and no cure. The olfactory bulb, involved in processing olfactory information, is affected early in the disease. The study demonstrates that dopamine metabolite DOPAL can replicate Parkinson's pathology, shedding light on the disease mechanism.
Article
Cell & Tissue Engineering
Elloise Z. Matthews, Stuart Lanham, Kate White, Maria-Eleni Kyriazi, Konstantina Alexaki, Afaf H. El-Sagheer, Tom Brown, Antonios G. Kanaras, Jonathan West, Ben D. MacArthur, Patrick S. Stumpf, Richard O. C. Oreffo
Summary: Researchers have developed a new method to isolate and enrich skeletal stem cells from human bone marrow, which have the potential to differentiate into bone, cartilage, and fat cells. This study provides valuable insights for further understanding and utilization of these stem cells.
JOURNAL OF TISSUE ENGINEERING
(2023)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.
