Mixed peptide-chitosan membranes to mimic the biological activities of a multifunctional laminin α1 chain LG4 module

Laminin alpha 1 chain LG4 module is multifunctional and interacts with syndecans and integrin alpha 2 beta 1 via AG73 (RKRLQVQLSIRT) and EF-1 (DYATLQLQEGRLHFMFDLG) sites, respectively. Here, We Conjugated the AG73 and EF1zz (ATLQLQEGRLHFXFDLGKGR, X: Nle) peptides on a chitosan membrane in various ratios to develop an LG4 mimic biomaterial. The AG73-chitosan membrane promoted strong cell attachment with membrane ruffling and the EF1zz-chitosan membrane promoted integrin-mediated cell adhesion with well-organized actin stress fibers. When AC73 and EF1zz were Conjugated on a chitosan membrane with 1:9 molar ratio, the mixed peptide-chitosan membrane promoted the strong cell attachment and neurite Outgrowth similar to that Oil the recombinant LG4 protein. Well-organized actin stress fibers and vinculin accumulated focal contacts were observed in the cells attached on the AG73:EF1zz (molar ratio= 1:9)-chitosan membrane. These results suggest that the mixed peptide-chitosan membrane interacts with both syndecans and integrin alpha 2 beta 1 and mimics the cell adhesion of a Multifunctional LG4 protein. The mixed peptide-chitosan approach has potential as a multifunctional biomaterial for cell and tissue engineering. (C) 2008 Elsevier Ltd. All rights reserved.