Article
Biochemistry & Molecular Biology
Wenzhao Li, Cheng Tao, Minzhi Mao, Kewei Zhu
Summary: The study investigates the role of Nrf2 and its downstream pathway in osteoarthritis (OA). It is found that Nrf2 functions as a regulator, suppressing inflammation and modulating cell apoptosis, extracellular matrix degradation, and NF-kappa B signaling in chondrocytes and OA mice. These findings provide insights for potential therapeutic targets in OA treatment.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2023)
Article
Orthopedics
Y. He, M. Zhang, J. Song, M. L. Warman
Summary: The study showed that cell-depleted areas within MCC cartilage are not repopulated by new cells in adult mice after diphtheria toxin-mediated chondrocyte killing.
OSTEOARTHRITIS AND CARTILAGE
(2021)
Article
Engineering, Biomedical
Yong Kwan Noh, Sung Won Kim, Ik-Hwan Kim, Kwideok Park
Summary: The study demonstrated that cells cultured on the natural form of decellularized ECM, N-CHDM, exhibited better proliferation and differentiation properties, with higher levels of chondrogenic marker expression and immunofluorescence of COL II. Moreover, NSCs preconditioned on N-CHDM showed higher cell retention rates, more cells with chondrocyte lacunae structure, and increased production of cartilage ECM when transplanted in nude mice.
BIOMATERIALS RESEARCH
(2021)
Article
Cell Biology
Carlos Martinez-Armenta, Carlos Suarez-Ahedo, Anell Olivos-Meza, Maria C. Camacho-Rea, Laura E. Martinez-Gomez, Guadalupe Elizabeth Jimenez-Gutierrez, Gabriela A. Martinez-Nava, Luis E. Gomez-Quiroz, Carlos Pineda, Alberto Lopez-Reyes
Summary: In this study, a scaffold-free 3D culture system was developed to generate spheroids from human chondrocytes. The results showed that spheroids developed in a hypoxic environment exhibited better chondrogenesis characteristics compared to those developed under normoxic conditions. This novel culture system could be a valuable tool for cartilage biology research.
Review
Chemistry, Medicinal
Jhi Biau Foo, Qi Hao Looi, Chee Wun How, Sau Har Lee, Maimonah Eissa Al-Masawa, Pei Pei Chong, Jia Xian Law
Summary: Exosomes are small extracellular vesicles secreted by cells for intercellular communication, with miRNAs being a promising therapeutic factor for cartilage regeneration. They can promote chondrocyte proliferation and matrix secretion, reduce scar tissue formation, and decrease inflammation, but the optimization of exosome therapy for cartilage injuries is still lacking.
Article
Materials Science, Biomaterials
Cynthia M. Co, Samira Izuagbe, Jun Zhou, Ning Zhou, Xiankai Sun, Joseph Borrelli, Liping Tang
Summary: A novel cell-based therapy has been developed for treating articular cartilage injuries, involving the pre-targeting of apoptotic chondrocytes and the delivery of healthy, metabolically active chondrocytes using click chemistry. The effectiveness of this approach was demonstrated in reducing cartilage degeneration and enhancing ECM regeneration in an ex vivo human cartilage explant PTOA model. This Tz-TCO click chemistry-mediated cell delivery approach shows great potential in clinical applications for targeting and treating cartilage injury.
REGENERATIVE BIOMATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Elizabeth R. Kahle, Biao Han, Prashant Chandrasekaran, Evan R. Phillips, Mary K. Mulcahey, X. Lucas Lu, Michele S. Marcolongo, Lin Han
Summary: Molecular engineering using synthetic mimics of native matrix molecules can modulate the mechanical properties of cellular microenvironment and influence cell mechanobiology. This study demonstrated the use of biomimetic proteoglycans (BPGs) to engineer the micromechanics of the pericellular matrix (PCM) in cartilage, leading to enhanced cellular mechanotransduction. The interactions between BPGs and the native PCM were facilitated by the biomimetic ultrastructure of BPGs, and showed potential for improving tissue regeneration and disease modification in various cell types.
Article
Endocrinology & Metabolism
Weirong Xing, Sheila Pourteymoor, Yian Chen, Subburaman Mohan
Summary: Studies show that targeted deletion of the Cldn12 gene in mice increases articular cartilage, potentially by promoting articular chondrocyte phenotype.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Oncology
Evgenia Karousou, Arianna Parnigoni, Paola Moretto, Alberto Passi, Manuela Viola, Davide Vigetti
Summary: The extracellular matrix has a complex structure and the glycans within are difficult to study, but they have various effects on cells, especially the glycosaminoglycan hyaluronan. Changes in hyaluronan concentration or dimension can affect tissue stiffness and cellular response through membrane receptors. Hyaluronan plays a role in cancer progression and can be affected by growth factors and cell metabolism.
Article
Biotechnology & Applied Microbiology
Yu Seon Kim, Shail M. Mehta, Jason L. Guo, Hannah A. Pearce, Brandon T. Smith, Emma Watson, Gerry L. Koons, Adam M. Navara, Johnny Lam, K. Jane Grande-Allen, Antonios G. Mikos
Summary: This study investigated the chondrogenic activity of encapsulated MSCs and ACs in hydrogels, as well as the impact of PLL on their mechanical properties. The highest chondrogenic activity was observed in AC-encapsulated hydrogels, while no significant differences were found in mechanical properties among the study groups. The results suggest that the ex vivo model can provide insights into the relationship between biochemical changes and mechanical properties in hydrogels.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Review
Dermatology
Romana Sinova, Vojtech Pavlik, Martin Ondrej, Vladimir Velebny, Kristina Nesporova
Summary: The study provides an overview of the literature concerning changes in hyaluronan in photoaged skin, as well as its potential role in photoaging. It suggests novel contributions of hyaluronan based on its role in other pathological processes, and discusses potential interventions targeting hyaluronan metabolism to mitigate photoaging.
EXPERIMENTAL DERMATOLOGY
(2022)
Article
Cell Biology
Junjie Wang, Zewen Sun, Chenghao Yu, Haibo Zhao, Mingyue Yan, Shenjie Sun, Xu Han, Tingting Jiang, Tianrui Wang, Tengbo Yu, Yingze Zhang
Summary: This study used single-cell RNA sequencing to analyze chondrocytes under different force conditions. Nine chondrocyte subsets were identified, and differences between the AT and ATa groups were analyzed. The results showed that force activated reactive oxygen species related processes and tissue repair processes were common in the force-bearing region. Prehypertrophic chondrocytes seemed to play an important role in the ankle.
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Yan Lu, Yuxiang Wang, Hanjie Zhang, Zizhao Tang, Xiaolin Cui, Xing Li, Jie Liang, Qiguang Wang, Yujiang Fan, Xingdong Zhang
Summary: The study demonstrated that functionalizing collagen scaffolds with porcine decellularized extracellular matrix can promote neocartilage regeneration. Results showed that the solution form of ECM performed better in cell recruitment, proliferation, and chondrogenesis, quickly providing a favorable chondrogenic microenvironment for endogenous BMSCs.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Review
Biochemistry & Molecular Biology
Ilias Ektor Epanomeritakis, Ernest Lee, Victor Lu, Wasim Khan
Summary: Focal chondral defects of the knee are common in young, active individuals, and cell-seeded scaffolds have shown promising results in repairing these defects. Most patients achieved complete integration with native cartilage using cell-seeded scaffolds, and the integration quality improved over time.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Medicine, Research & Experimental
Xinyue Zhang, Xiaohua Pu, Caixia Pi, Jing Xie
Summary: FGF7, also known as KGF, plays a crucial role in tissue development, wound repair, tumorigenesis, and immune reconstruction. It directs cellular synaptic extension and facilitates functional gap junction communication in the skeletal system. Additionally, it promotes osteogenic differentiation and regulates key molecules in cartilage. However, the molecular mechanism of FGF7 in chondrocyte behaviors and cartilage diseases remains unknown.
Article
Veterinary Sciences
E. J. O. O'Brien, J. M. McAlpine, M. C. Schramme, P. D. Clegg, R. M. Archer, C. De Guio, R. K. W. Smith
EQUINE VETERINARY EDUCATION
(2018)
Article
Biotechnology & Applied Microbiology
Agnieszka J. Turlo, Yalda Ashraf Kharaz, Peter D. Clegg, James Anderson, Mandy J. Peffers
Article
Orthopedics
Katie Joanna Lee, Peter David Clegg, Eithne Josephine Comerford, Elizabeth Gail Canty-Laird
BMC MUSCULOSKELETAL DISORDERS
(2018)
Editorial Material
Physiology
Simon R. Tew, Richard Barrett-Jolley
EXPERIMENTAL PHYSIOLOGY
(2018)
Article
Anatomy & Morphology
Yalda A. Kharaz, Elizabeth G. Canty-Laird, Simon R. Tew, Eithne J. Comerford
JOURNAL OF ANATOMY
(2018)
Meeting Abstract
Orthopedics
B. T. McDermott, M. J. Peffers, B. McDonagh, S. R. Tew
OSTEOARTHRITIS AND CARTILAGE
(2018)
Meeting Abstract
Orthopedics
B. T. McDermott, K. A. Johnson, H. F. Hayati, S. R. Tew
OSTEOARTHRITIS AND CARTILAGE
(2018)
Meeting Abstract
Orthopedics
A. Smagul, S. Tew, M. J. Peffers
OSTEOARTHRITIS AND CARTILAGE
(2018)
Meeting Abstract
Orthopedics
M. Barrow, S. Tew, E. Comerford, M. McNicholas, R. Oldershaw
OSTEOARTHRITIS AND CARTILAGE
(2018)
Review
Cell & Tissue Engineering
Melissa Lo Monaco, Greet Merckx, Jessica Ratajczak, Pascal Gervois, Petra Hilkens, Peter Clegg, Annelies Bronckaers, Jean-Michel Vandeweerd, Ivo Lambrichts
STEM CELLS INTERNATIONAL
(2018)
Review
Geriatrics & Gerontology
Graham J. Kemp, Fraser Birrell, Peter D. Clegg, Daniel J. Cuthbertson, Giuseppe De Vito, Jaap H. van Dieen, Silvia Del Din, Richard Eastell, Patrick Garnero, Katarzyna Goljanek-Whysall, Matthias Hackle, Richard Hodgson, Malcolm J. Jackson, Sue Lord, Claudia Mazza, Anne McArdle, Eugene V. McCloskey, Marco Narici, Mandy J. Peffers, Stefano Schiaffino, John C. Mathers
Article
Orthopedics
Rhiannon E. Morgan, Peter D. Clegg, John A. Hunt, John F. Innes, Simon R. Tew
JOURNAL OF ORTHOPAEDIC RESEARCH
(2018)
Article
Biochemistry & Molecular Biology
Benjamin T. McDermott, Mandy J. Peffers, Brian McDonagh, Simon R. Tew
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Cell & Tissue Engineering
O. J. Ali, E. J. Comerford, P. D. Clegg, E. G. Canty-Laird
EUROPEAN CELLS & MATERIALS
(2018)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.