Article
Biology
Lucia Sanjurjo, Iris A. Schulkens, Pauline Touarin, Roy Heusschen, Ed Aanhane, Kitty C. M. Castricum, Tanja D. De Gruijl, Ulf J. Nilsson, Hakon Leffler, Arjan W. Griffioen, Latifa Elantak, Rory R. Koenen, Victor L. J. L. Thijssen
Summary: The study reveals that specific chemokine-galectin interactions can modulate the immunoregulatory function of galectins, affecting their glycan-binding activity and specificity. In particular, binding of CXCL4 changes the carbohydrate binding site of galectin-1, increasing its apoptotic activity on CD8(+) T cells.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Michaela Hovorkova, Jakub Cerveny, Ladislav Bumba, Helena Pelantova, Josef Cvacka, Vladimir Kren, Olivier Renaudet, David Goyard, Pavla Bojarova
Summary: A new type of glycoconjugate with low nanomolar affinity was developed to inhibit galectins of interest for biomedical research. The presentation of carbohydrate ligands on specific structural scaffolds was found to impact the affinity and selectivity to different galectins.
BIOORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Monika Mullerova, Michaela Hovorkova, Tana Zavodna, Lucie Cervenkova Stastna, Alena Krupkova, Vojtech Hamala, Katerina Novakova, Jan Topinka, Pavla Bojarova, Tomas Strasak
Summary: Galectins and their carbohydrate ligands play a vital role in regulating various biological processes. Carbosilane dendrimers show promise as a nanoscaffold for presenting multivalent carbohydrate ligands to target galectin receptors. This study presents a synthetic method for lactose-functionalized carbosilane glycodendrimers and demonstrates their high affinity to galectin-9. Multivalent presentation of the ligand significantly enhances its inhibitory effect, as shown by the 1400-fold higher potency of the third-generation dendritic ligand compared to monovalent lactose. The increased affinity is attributed to the formation of stable galectin/lactose-functionalized carbosilane glycodendrimer aggregates.
Review
Reproductive Biology
Xuejing Pei, Jiahui Zhu, Yuchen Wang, Fali Zhang, Yufeng He, Yuchun Li, Yunlong Si
Summary: Placental galectins are a unique family of proteins that play important roles in placental tissue and have received significant attention due to their distinctive structural features and loss of lactose binding ability. Understanding the functions of placental galectins may contribute to the discovery of new mechanisms causing pregnancy diseases and the development of new diagnostic and therapeutic strategies.
BIOLOGY OF REPRODUCTION
(2023)
Review
Immunology
Fu-Tong Liu, Sean R. Stowell
Summary: Galectins can modulate immune cells by binding to glycosylated proteins and lipids on the cell surface, or intracellularly via carbohydrate-dependent or carbohydrate-independent interactions. This Review explores the diverse ways in which galectins affect immunity and discusses the opportunities and challenges in the field.
NATURE REVIEWS IMMUNOLOGY
(2023)
Article
Gastroenterology & Hepatology
Yin Tong, Priscilla S. W. Cheng, Chung Sze Or, Sarah S. K. Yue, Hoi Cheong Siu, Siu Lun Ho, Simon Y. K. Law, Wai Yin Tsui, Dessy Chan, Stephanie Ma, Siu Po Lee, Annie S. Y. Chan, April S. Chan, Shui Wa Yun, Ho Sang Hui, Siu Tsan Yuen, Suet Yi Leung, Helen H. N. Yan
Summary: Cell-cell and cell-matrix adhesions play a critical role in gastric cancer progression, especially in gastric cancer with dysregulated RHO signaling.
Review
Chemistry, Medicinal
Aaftaab Sethi, Swetha Sanam, Mallika Alvala
Summary: Lectins are a diverse family of glycan-binding proteins, designing efficient carbohydrate inhibitors is challenging; Galectins, a sub-family of lectins, are recognized as crucial targets for various diseases; Many candidates targeting galectins are currently in advanced stages of clinical trials.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Ruud P. M. Dings, Nigam Kumar, Sterling Mikkelson, Hans-Joachim Gabius, Kevin H. Mayo
Summary: The study found that members of the galectin family may exhibit more than additive effects in mixtures, prompting further investigation into their functional activities. Testing galectins in mixtures can simulate the situation of co-expression in situ and reveal unsuspected over-additive activities.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Victor L. Thijssen
Summary: Angiogenesis, the process of new blood vessel growth, is tightly regulated and involves endothelial cells adopting different phenotypes to proliferate, migrate, and form tube-like structures. The galectin protein family is crucial for proper angiogenesis execution and has been linked to endothelial cell function and various steps of the angiogenesis cascade.
Article
Cell Biology
Belinda L. Spillings, Christopher J. Day, Albert Garcia-Minambres, Anupriya Aggarwal, Nicholas D. Condon, Thomas Haselhorst, Damian F. J. Purcell, Stuart G. Turville, Jennifer L. Stow, Michael P. Jennings, Johnson Mak
Summary: This study reveals that HIV is captured near the lymphocyte surface in a virion-glycan-dependent manner. Removing certain glycans impairs virus-cell binding and interfering with glycan interactions reduces HIV infectivity. These findings provide new insights into the interaction between HIV and host cells.
Article
Biochemistry & Molecular Biology
Maria Vougioukalaki, Konstantina Georgila, Emmanouil Athanasiadis, Aristides G. Eliopoulos
Summary: This study reveals that detached carcinoma cells and immortalized fibroblasts exhibit defects in signaling pathways induced by TNF and CD40L, leading to increased sensitivity to death-inducing receptor ligands. The findings suggest a new mode of regulation of TNF family signal transduction by adhesion-related molecules, which may have significant implications for cancer therapy.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Medicine, Research & Experimental
Qiu-Yang Jin, Ying-Shuang Li, Xing-Hui Qiao, Jia-Wei Yang, Xiu-Li Guo
Summary: Tumor immunotherapy has emerged as an innovative and advantageous treatment method, but still requires assistance for clinical application due to its complexity. Galectins modulated in the tumor microenvironment can regulate immune system disorders to resist tumor growth, suggesting that combination therapy of targeted anti-galectins with immunotherapy may be promising for tumor treatment. This review discusses the expression and immune functions of different galectins in tumor microenvironment, highlighting the potential value of anti-galectins in combination with checkpoint inhibitors for tumor immunotherapy.
Review
Chemistry, Medicinal
Chih-Yen Lin, Zih-Syuan Yang, Wen-Hung Wang, Aspiro Nayim Urbina, Yu-Ting Lin, Jason C. Huang, Fu-Tong Liu, Sheng-Fan Wang
Summary: Currently, only Galectin-1, Galectin-3, and Galectin-9 have been reported to play a regulatory role in IAV infection. They inhibit IAV infection through multiple mechanisms, including blocking virus-host receptor interaction, activating inflammasome, and increasing expression of antiviral genes and cytokines.
Article
Cell Biology
Shaowei Mao, Di Zhang, Luan Chen, Jie Tan, Yunpeng Chu, Sijia Huang, Wenqi Zhou, Hengwei Qin, Qinghua Xia, Yueran Zhao, Rongxiu Li, Shengying Qin, Muyun Wei
Summary: Metastasis is a serious risk for RCC patients and understanding the molecular mechanisms underlying it is crucial for developing new therapeutic approaches. Studies have shown that FKBP51 may play a role in regulating ECM function and promoting invasion and migration of tumor cells in RCC. The study found that FKBP51 promotes ccRCC invasion and migration by binding with TIMP3, connecting it with Beclin1 complex and increasing autophagic degradation of TIMP3.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Yasuhiro Nonaka, Takashi Ogawa, Hiroki Shoji, Nozomu Nishi, Shigehiro Kamitori, Takanori Nakamura
Summary: This study elucidated the key residues for carbohydrate specificity of Xenopus laevis skin galectin xgalectin-Va through site-directed mutagenesis, leading to a successful change in carbohydrate selectivity. The mutation of two amino acids greatly enhanced the binding ability to N-acetyllactosamine and conferred significant T-cell growth inhibition activity. The loop substitution also successfully altered the carbohydrate selectivity of xgalectin-Va and xgalectin-Ib.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2021)
Article
Cell Biology
Gabriel Garcia Caballero, Donella Beckwith, Nadezhda Shilova, Adele Gabba, Tanja J. Kutzner, Anna-Kristin Ludwig, Joachim C. Manning, Herbert Kaltner, Fred Sinowatz, Mare Cudic, Nicolai Bovin, Paul Murphy, Hans-Joachim Gabius
HISTOCHEMISTRY AND CELL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Kira Dobrochaeva, Nailya Khasbiullina, Nadezhda Shilova, Nadezhda Antipova, Polina Obukhova, Oxana Galanina, Mikhail Gorbach, Inna Popova, Sergey Khaidukov, Natalia Grishchenko, Nikolai Tupitsyn, Jacques Le Pendu, Nicolai Bovin
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Multidisciplinary Sciences
Sergio A. Machado, Momal Sharif, Govindasamy Kadirvel, Nicolai Bovin, David J. Miller
Article
Virology
Natalia A. Ilyushina, Nicolette Lee, Vladimir Y. Lugovtsev, Anastasia Kan, Nicolai Bovin, Raymond P. Donnelly
Article
Biochemistry & Molecular Biology
Roman A. Kunetskiy, Galina Pazynina, Igor A. Ivanov, Nicolai Bovin
CARBOHYDRATE RESEARCH
(2020)
Review
Biochemistry & Molecular Biology
Alexander Tuzikov, Alexander Chinarev, Nadezhda Shilova, Elena Gordeeva, Oxana Galanina, Tatyana Ovchinnikova, Marcel Schaefer, Nicolai Bovin
Summary: Polyacrylamide conjugates of glycans are widely used in glycobiology for immobilizing glycans and as multivalent inhibitors. The biotin tag allows quantitative immobilization on any surface and acts as a tracer for detecting carbohydrate-binding proteins. Additionally, this review covers less common but significant applications, as well as details on the glycopolymers themselves and their interactions with proteins.
GLYCOCONJUGATE JOURNAL
(2021)
Article
Medicine, Research & Experimental
Kira Dobrochaeva, Nailya Khasbiullina, Nadezhda Shilova, Yuriy Knirel, Polina Obukhova, Alexey Nokel, Roman Kunetskiy, Svetlana Tsygankova, Daniel Bello-Gil, Cristina Costa, Rafael Manez, Nicolai Bovin
Summary: The study compared the specificity of alpha-Gal antibodies in mice and humans, finding that the mouse model recognizes a broader range of human blood group B epitopes and glycans without the alpha-galactosyl residue compared to human antibodies, which show a preference for binding bacterial polysaccharides. This suggests caution should be exercised in using the mouse model in xenotransplantation experiments when epitope specificity is critical.
XENOTRANSPLANTATION
(2021)
Article
Hematology
Radhika Nagappan, Willy A. Flegel, Kshitij Srivastava, Eleanor C. Williams, Ivan Ryzhov, Alexander Tuzikov, Oxana Galanina, Nadezhda Shilova, Gennady Sukhikh, Holly Perry, Nicolai V. Bovin, Stephen M. Henry
Summary: This study evaluated the feasibility of using SARS-CoV-2 peptide Kode Technology-modified red cells to develop an assay for detecting SARS-CoV-2 antibodies. The results showed that C19-kodecytes have high specificity and sensitivity on routine serologic platforms, making them suitable for serological testing related to COVID-19.
Article
Microbiology
Adrien Breiman, Nathalie Ruvoen-Clouet, Marie Deleers, Tiffany Beauvais, Nicolas Jouand, Jezabel Rocher, Nicolai Bovin, Nathalie Labarriere, Hanane El Kenz, Jacques Le Pendu
Summary: The study reveals that COVID-19 patients have significantly lower levels of anti-Tn antibodies compared to non-infected individuals, indicating both quantitative and qualitative differences. The anti-Tn antibodies are correlated with the development of specific antiviral response and are also lower in individuals with blood types A and B.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Developmental Biology
Momal Sharif, Karl Kerns, Peter Sutovsky, Nicolai Bovin, David J. Miller
Summary: Studies have shown that progesterone-induced sperm release requires active CatSper channels and proteasomal degradation, indicating that hyperactivation and proteolysis are vital parts of the mechanism for sperm movement.
Article
Chemistry, Organic
Alexey V. Nizovtsev, Nicolai V. Bovin
Summary: A new convenient synthetic route for 2-aminopropane-1,2,3-tricarboxylic acid is described, involving two initial steps conducted in a one-pot procedure. The successful synthesis is achieved through the cyclization of hippuric acid and subsequent hydrolysis with HCl.
SYNTHESIS-STUTTGART
(2021)
Article
Immunology
Marina M. Ziganshina, Nadezhda Shilova, Nailia R. Khasbiullina, Anastasia Terentyeva, Elena L. Dolgopolova, Alexey Yu Nokel, Ekaterina L. Yarotskaya, Roman G. Shmakov, Nicolai Bovin, Gennady T. Sukhikh
Summary: A possible mechanism of immune tolerance in pregnancy is the production of blocking antibodies in the placenta, which protect the fetus from the mother's immune system. The specificity and nature of these antibodies are still unknown. However, the study found that the repertoire of placental antibodies is less variable and qualitatively different from those in the peripheral blood, and this repertoire is narrower in women with pre-eclampsia compared to healthy pregnancy. Some antibodies are found exclusively in the placenta, while others are only found in the placenta of healthy women.
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
E. Slivka, A. B. Tuzikov, S. Khaidukov, V. A. Komarova, S. M. Henry, N. Bovin, E. M. Rapoport
Summary: This study compared the properties of three synthetic analogs and found that they have similar localization patterns and can be interchanged when studying the incorporation/release processes. However, there are some differences in the insertion process and release kinetics.
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY
(2022)
Review
Nanoscience & Nanotechnology
A. Yu Nokel, N. Bovin, N. Shilova
Summary: The review discusses the main methods for modifying plastic as a functional material for manufacturing biochips, including physical, chemical, and physical-chemical methods for introducing functional groups and achieving effective immobilization of biomolecules. The advantages and disadvantages of existing approaches are discussed in terms of their applicability to various tasks.
NANOBIOTECHNOLOGY REPORTS
(2022)
Article
Immunology
Holly E. Perry, Ivan Ryzhov, Oxana Galanina, Nicolai Bovin, Stephen M. Henry
AIMS ALLERGY AND IMMUNOLOGY
(2020)
