Article
Biochemistry & Molecular Biology
Zhanying Wu, Biao Li, Kaiwei Yu, Nana Zheng, Feifei Yuan, Jingjing Miao, Meijia Zhang, Zhijuan Wang
Summary: Porcine spermatozoa are stored in the oviductal isthmus after natural mating, and the number of spermatozoa is increased in the oviductal ampulla when the mature cumulus-oocyte complexes (COCs) are transferred into the ampulla. The study found that natriuretic peptide type C (NPPC) and its receptor natriuretic peptide receptor 2 (NPR2) play a role in sperm release from oviduct istsmic cells. The mature COCs promote NPPC expression in the ampulla through TGF-beta signaling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Yueshuai Liu, Hongxiang Ding, Yuze Yang, Yan Liu, Xin Cao, Tao Feng
Summary: This study investigated the effects of progesterone treatment on apoptosis and steroidogenesis in porcine placental trophoblasts and explored the underlying molecular mechanisms. The results showed that high doses of progesterone inhibited trophoblast proliferation and significantly affected the expression of apoptosis-related and steroidogenesis-related genes and proteins. Low doses of progesterone had a milder effect. The study contributes to the understanding of the effects of progesterone on porcine placental trophoblast functions, which are crucial for pregnancy and maternal-fetal health.
Article
Oncology
Maria V. Yusenko, Abhiruchi Biyanee, Mattias K. Andersson, Silke Radetzki, Jens P. von Kries, Goran Stenman, Karl-Heinz Klempnauer
Summary: Studies on MYB in human malignancies have identified it as a potential drug target for AML and ACC. While transcription factors are often considered hard to target, recent developments have shown successful targeting of MYB with low molecular weight compounds. The use of proteasome inhibitors to suppress oncogenic MYB activity opens up new possibilities for therapeutic approaches in MYB-driven cancers.
Article
Immunology
Chunxiao Mou, Yuxi Wang, Shuonan Pan, Kaichuang Shi, Zhenhai Chen
Summary: This research investigated the cell apoptosis induced by Porcine sapelovirus (PSV) infection and its underlying mechanisms. The results showed typical features of apoptosis, such as nuclear condensation and fragmentation, after infection. Cell apoptosis was confirmed by analyzing apoptotic rates, caspase activation, and PARP1 cleavage. The study found that 2A protein encoded by PSV was a crucial factor in inducing the mitochondrial apoptotic pathway.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Cell Biology
Supipi Mirihagalle, Jennifer Rose Hughes, David Joel Miller
Summary: This article discusses the function and mechanism of the sperm reservoir in the female reproductive system of mammals. The interaction between glycans and sperm membrane proteins facilitates sperm binding and retention in the oviductal epithelium, and progesterone has been identified as a signaling molecule that induces the release of sperm from the reservoir.
Article
Biochemistry & Molecular Biology
Ajamaluddin Malik, Abdullah Alhomida, Javed Masood Khan
Summary: This study investigated the influence of a negatively charged benzene ring containing surfactant, sodium dodecylbenzene sulphonate (SDBS), on the fibrillation of bovine liver catalase (BLC). It was found that lower concentrations of SDBS promoted the native-like alpha-helical structure of BLC, while higher concentrations led to aggregation and the formation of amyloid-like structures. At pH 2.0, higher concentrations of SDBS enabled BLC to regain its native-like alpha-helical structure.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Reproductive Biology
Jennifer R. Hughes, Katie J. McMorrow, Nicolai Bovin, David J. Miller
Summary: The binding of porcine sperm to a specific oviduct glycan diminishes the activity of the electron transport chain and reduces reactive oxygen species production. This interaction prolongs the lifespan of porcine sperm and may be due to the suppression of harmful reactive oxygen species. The storage of sperm in the lower oviduct allows for asynchrony between mating and ovulation.
BIOLOGY OF REPRODUCTION
(2023)
Article
Biochemistry & Molecular Biology
Taisiya R. Yurakova, Ekaterina O. Gubernatorova, Ekaterina A. Gorshkova, Maxim A. Nosenko, Sergei A. Nedospasov, Marina S. Drutskaya
Summary: This study explores the immune-metabolic reprogramming of myeloid cells in the development of asthma and identifies the association between a specific immunometabolic phenotype, mitochondrial morphology, ROS production in macrophages, and the TLR4-dependent processes. These findings provide insight into the molecular mechanisms underlying asthma and may offer potential therapeutic strategies for its treatment.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Claudia Sanchez-Cardenas, Ana Romarowski, Gerardo Orta, Jose Luis De la Vega-Beltran, David Martin-Hidalgo, Arturo Hernandez-Cruz, Pablo E. Visconti, Alberto Darszon
Summary: The study found that starvation increases intracellular Ca2+ levels and enhances the sperm's ability to undergo a progesterone-induced acrosome reaction. The CatSper channel remains active in starvation conditions, but an increase in Ca2+ levels was also observed in sperm from CatSper null mice. Addition of energy nutrients can reverse the effects of starvation.
Article
Immunology
D. Alejandro Canaria, J. Alejandra Rodriguez, Luopin Wang, Franklin J. Yeo, Bingyu Yan, Mengbo Wang, Charlotte Campbell, Majid Kazemian, Matthew R. Olson
Summary: Tox is expressed in various T cell subtypes and promotes the differentiation of Th2 and Treg cells. Its overexpression induces the expression of genes involved in cell activation, cellular trafficking, and inflammation suppression. Tox regulates the transcription of these genes along with BATF, IRF4, and JunB.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Guoliang Zhang, Yiqing He, Yiwen Liu, Yan Du, Cuixia Yang, Feng Gao
Summary: The study revealed that the deficiency of cross-linked HA induced breast cancer malignancy in a CAF-dependent manner, with CAFs restoring high levels of cross-linked HA and inhibiting malignancy through upregulating TSG6. These findings suggest that recovering HA cross-linking may be a potential therapeutic strategy.
CELL DEATH & DISEASE
(2021)
Article
Cell Biology
Can Huang, Patricia Santofimia-Castano, Xi Liu, Yi Xia, Ling Peng, Celia Gotorbe, Jose Luis Neira, Daolin Tang, Jacques Pouyssegur, Juan Iovanna
Summary: Experimental results demonstrate that ZZW-115-induced ferroptosis mediated by NUPR1 inhibitor can be rescued by Fer-1 and TFAM supplementation, triggering ferroptotic activity in cells derived from PDAC and HCC. Additionally, ZZW-115 treatment leads to alterations in mitochondrial morphology and function, which can be rescued by Fer-1 and NAC.
CELL DEATH DISCOVERY
(2021)
Article
Genetics & Heredity
Sydney C. Vanderkooi, Yuewen Zhao, Patricia D. A. Lima, Frederick W. K. Kan
Summary: This study reveals that rHuOVGP1 may work with P4 to increase intracellular calcium concentration at the beginning of sperm capacitation, partly through CatSper channels, which leads to tyrosine phosphorylation of sperm proteins and enhancement of sperm capacitation.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)
Article
Endocrinology & Metabolism
Amber L. Cathey, Deborah J. Watkins, Zaira Y. Rosario, Carmen M. Velez Vega, Bhramar Mukherjee, Marie S. O'Neill, Rita Loch-Caruso, Akram N. Alshawabkeh, Jose F. Cordero, John D. Meeker
Summary: This study found that higher concentrations of certain hormones during pregnancy were associated with shorter gestational age at birth for male fetuses, as well as increased odds of preterm birth for males with specific hormone concentrations. Conversely, higher testosterone concentrations were associated with increased odds of preterm birth for female fetuses. The study suggests that hormonal associations with timing of delivery may vary by fetal sex, highlighting the need for further research to understand the mechanisms involved.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Costanza Cimini, Fadl Moussa, Angela Taraschi, Marina Ramal-Sanchez, Alessia Colosimo, Giulia Capacchietti, Samia Mokh, Luca Valbonetti, Israiel Tagaram, Nicola Bernabo, Barbara Barboni
Summary: This study investigated the impact of oviductal epithelial cells and progesterone hormone on sperm fertilizing ability using an in vitro fertilization (IVF) swine model. The results showed that pre-treated oviductal epithelial cells with 100 ng/mL progesterone promoted sperm capacitation and increased the fertilizing ability without hormone addition at the time of fertilization.
Article
Microbiology
Adrien Breiman, Nathalie Ruvoen-Clouet, Marie Deleers, Tiffany Beauvais, Nicolas Jouand, Jezabel Rocher, Nicolai Bovin, Nathalie Labarriere, Hanane El Kenz, Jacques Le Pendu
Summary: The study reveals that COVID-19 patients have significantly lower levels of anti-Tn antibodies compared to non-infected individuals, indicating both quantitative and qualitative differences. The anti-Tn antibodies are correlated with the development of specific antiviral response and are also lower in individuals with blood types A and B.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Marina M. Ziganshina, Nadezhda Shilova, Nailia R. Khasbiullina, Anastasia Terentyeva, Elena L. Dolgopolova, Alexey Yu Nokel, Ekaterina L. Yarotskaya, Roman G. Shmakov, Nicolai Bovin, Gennady T. Sukhikh
Summary: A possible mechanism of immune tolerance in pregnancy is the production of blocking antibodies in the placenta, which protect the fetus from the mother's immune system. The specificity and nature of these antibodies are still unknown. However, the study found that the repertoire of placental antibodies is less variable and qualitatively different from those in the peripheral blood, and this repertoire is narrower in women with pre-eclampsia compared to healthy pregnancy. Some antibodies are found exclusively in the placenta, while others are only found in the placenta of healthy women.
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Momal Sharif, Vincent Hickl, Gabriel Juarez, Xingjian Di, Karl Kerns, Peter Sutovsky, Nicolai Bovin, David J. Miller
Summary: This study demonstrates that inducing sperm hyperactivation is sufficient to release sperm from immobilized oviduct glycans, and the release is dependent on CatSper and the ubiquitin-proteasome system.
SCIENTIFIC REPORTS
(2022)
Review
Agriculture, Dairy & Animal Science
Mohammed S. Liman, Abubeker Hassen, Lyndy J. McGaw, Peter Sutovsky, Dietmar E. Holm
Summary: This review examined the impact of semen cryopreservation and tannins on sperm function, highlighting the role of tannins in combating reactive oxygen species and promoting successful fertilization. Additionally, the effects of tannin extract feed supplementation on male fertility were investigated, and the potential benefits of tannins as additives in semen extenders were explored.
Article
Cell Biology
Eriklis Nogueira, Filip Tirpak, Lauren E. Hamilton, Michal Zigo, Karl Kerns, Miriam Sutovsky, JaeWoo Kim, Dietrich Volkmann, Luca Jovine, Jeremy F. Taylor, Robert D. Schnabel, Peter Sutovsky
Summary: This study aims to identify and phenotype rare, deleterious mutations that affect sperm quality or have high developmental and fertility potential. A rare homozygous mutation in EML5 was found in an Angus bull used extensively in artificial insemination. This mutation was also found in other breeds. The mutation may affect sperm morphology and the overall protein function of EML5. Further research will investigate the impact of this mutation on field AI fertility and the role of EML5 in spermatogenesis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biology
Michal Zigo, Karl Kerns, Sidharth Sen, Clement Essien, Richard Oko, Dong Xu, Peter Sutovsky
Summary: Zinc-interacting proteins in porcine sperm undergo changes during sperm maturation, particularly during sperm capacitation. These changes may be associated with Huntington's and Parkinson's disease pathways. The findings have implications for reproductive health, assisted reproductive therapy, and livestock breeding.
COMMUNICATIONS BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Meghan Lawlor, Michal Zigo, Karl Kerns, In Ki Cho, Charles A. Easley, Peter Sutovsky
Summary: Huntington's Disease is a fatal neurodegenerative disease with motor dysfunction and cognitive deficits. It also leads to reduced fertility. Although there are pharmaceuticals to manage its symptoms, an effective treatment for the disease is yet to be approved. Identification of genes, diagnostic biomarkers, and therapeutic target pathways could help in early diagnosis and management of the disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
E. Slivka, A. B. Tuzikov, S. Khaidukov, V. A. Komarova, S. M. Henry, N. Bovin, E. M. Rapoport
Summary: This study compared the properties of three synthetic analogs and found that they have similar localization patterns and can be interchanged when studying the incorporation/release processes. However, there are some differences in the insertion process and release kinetics.
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Michal Zigo, Karl Kerns, Peter Sutovsky
Summary: The present study focused on the role of the ubiquitin-proteasome system (UPS) in sperm capacitation. High-resolution, quantitative LC-MS analysis revealed differentially expressed proteins associated with sperm capacitation, hyperactivation, metabolism, acrosomal exocytosis, and fertilization. Further investigation confirmed the involvement of specific proteins, such as CSE1L, PFDN4, and STK39, in sperm capacitation. This research provides insights into potential pharmacological mechanisms to modulate sperm fertilizing ability in animals and humans.
Article
Cell Biology
J. T. Soley, L. du Plessis, M. Sutovsky, P. Sutovsky
Summary: This paper provides the first detailed description of the spermiogenesis process in ostriches and one of the few in any avian species. The steps of spermiogenesis were observed and illustrated using light microscopy and supplemented with ultrastructural observations, PNA labeling, and immunocytochemical labeling. Eight steps were identified, showing similarities with non-passerine birds. However, fewer steps were observed during the development of round spermatids compared to other bird species. This research has implications for comparative reproduction, animal science, and evolutionary biology.
CELL AND TISSUE RESEARCH
(2023)
Article
Biology
Dalen Zuidema, Alexis Jones, Won-Hee Song, Michal Zigo, Peter Sutovsky
Summary: The degradation of sperm-borne mitochondria after fertilization, known as post-fertilization sperm mitophagy, ensures exclusive maternal inheritance of mitochondrial DNA. This process involves the ubiquitin-proteasome system and other autophagic cofactors. The study used mass spectrometry and a porcine cell-free system to identify proteins involved in post-fertilization sperm mitophagy. The findings confirm the involvement of known determinants and provide a list of candidate cofactors for future studies.
Review
Nanoscience & Nanotechnology
A. Yu Nokel, N. Bovin, N. Shilova
Summary: The review discusses the main methods for modifying plastic as a functional material for manufacturing biochips, including physical, chemical, and physical-chemical methods for introducing functional groups and achieving effective immobilization of biomolecules. The advantages and disadvantages of existing approaches are discussed in terms of their applicability to various tasks.
NANOBIOTECHNOLOGY REPORTS
(2022)
