Article
Biochemistry & Molecular Biology
Yu-Ran Lee, Eun Young Bae, Hong Ryang Kil, Byeong-Hwa Jeon, Geena Kim
Summary: This study investigated the association between APE1/Ref-1 and Kawasaki disease (KD). The results showed that APE1/Ref-1 levels were significantly higher in KD patients, especially in refractory KD. APE1/Ref-1 could be a beneficial biomarker for the diagnosis and prognosis of KD.
Article
Biochemistry & Molecular Biology
Huanhuan Wang, Linghao Zhang, Huaqing Sun, Shufeng Xu, Kun Li, Xin Su
Summary: In this study, we developed an inhibitory aptamer for APE1 using SELEX technology. The aptamer showed high binding affinity and could entirely inhibit the activity of APE1. These aptamers can be utilized for early cancer diagnosis and treatment, as well as a valuable tool for studying the function of APE1.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Medicine, General & Internal
In Seol Yoo, Yu-Ran Lee, Seong Wook Kang, Jinhyun Kim, Hee-Kyoung Joo, Su-Jin Yoo, Chan Keol Park, Ha-Reum Lee, Ji Ah Park, Byeong-Hwa Jeon
Summary: The study demonstrates that the levels of APE1/Ref-1 are significantly elevated in the serum and synovial fluid of rheumatoid arthritis patients, and are positively correlated with disease activity.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Chemistry, Analytical
Yuqiang Hu, Zhen Zhang, Weicong Ye, Wei Zhang, Minghao Hu, Wenqian Yuan, Hongbo Wang, Xianjin Xiao, Tongbo Wu
Summary: Human apurinic/apyrimidinic endonuclease 1 (APE1) is a key player in DNA repair and gene regulation, with abnormal variations of its concentration in human blood and tissue cells being highly correlated to various diseases. This study introduces a DNA structure-mediated fluorescent biosensor for detecting APE1 activity with high sensitivity and selectivity. Through clever design, the biosensor improves APE1 reactivity and minimizes interference from other enzymes, achieving a low detection limit of 1.7 x 10(-6) U/mL and successful application in real biological samples and screening of APE1 inhibitors.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Yecan Pan, Rui Weng, Linghao Zhang, Jing Qiu, Xinlu Wang, Guangqin Liao, Zhaohui Qin, Lingpu Zhang, Haihua Xiao, Yongzhong Qian, Xin Su
Summary: Cellular oxidative stress caused by reactive oxygen species (ROS) and its induced molecular response is crucial in the development of various diseases and biological processes. By using DNA nanostructure-based fluorescent probes, the localization and abundance of ROS and its related molecules can be simultaneously mapped in living cells. This study introduces intramolecular Orthogonal Reporters (iOR) based on DNA nanostructure scaffolds, which eliminate undesired fluorophore interaction by precise positioning of fluorescence reporters. iOR, functionalized with nuclear localization signal peptide, enables high sensitivity and specificity in imaging ROS and its associated DNA repair enzyme (APE1) in the nucleus and cytoplasm. The study reveals a strong positive correlation and synergistic regulation between ROS and APE1 in living cells and tumor-bearing mice through direct fluorescence imaging. This work presents a new approach for developing nanostructure-based molecular probes with broad applications in cellular biology and nanotechnology.
Article
Biochemistry & Molecular Biology
Arianna Barchiesi, Veronica Bazzani, Agata Jabczynska, Lukasz S. Borowski, Silke Oeljeklaus, Bettina Warscheid, Agnieszka Chacinska, Roman J. Szczesny, Carlo Vascotto
Summary: APE1 is a versatile protein involved in DNA repair, gene expression regulation, and RNA metabolism in mitochondria. Loss of APE1 leads to accumulation of damaged mitochondrial mRNA, impairing protein translation and mitochondrial respiration efficiency. The data demonstrate that APE1 plays a crucial role in maintaining mitochondrial well-being.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Alexandra A. Kuznetsova, Svetlana Senchurova, Alexander A. Ishchenko, Murat Saparbaev, Olga S. Fedorova, Nikita A. Kuznetsov
Summary: The study revealed that structurally different AP endonucleases Nfo and APE1 utilize a common strategy of damage recognition controlled by enzyme conformational transitions after initial DNA binding. Local stability of the DNA duplex near the lesion affects catalytic-complex formation and DNA cleavage efficacy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Qi Kang, Xiaoyan Yang, Yumin Du, Yinxiao Qi, Zhiqiang He, Hua Xiang
Summary: In this study, a molecular gated hyperbranched rolling circle amplification (HRCA) quick sensing system was designed for rapid detection of APE1. The system exhibited rapid, simple, and sensitive APE1 detection and showed potential therapeutic applications for cancer treatment.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Zhe Hu, Weicong Ye, Zhen Zhang, Tianci Xie, Wenqian Yuan, Tongbo Wu
Summary: The AP site is a common DNA lesion and plays a critical role in the base excision repair pathway. Existing detection methods for AP sites are complicated and have high instrument requirements. Therefore, there is an urgent need for a sensitive and straightforward detection method without time-consuming and heterogeneous reactions.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Multidisciplinary
Jay S. Jha, Jiekai Yin, Tuhin Haldar, Zhiyu Yang, Yinsheng Wang, Kent S. Gates
Summary: The hydrolytic loss of coding bases from cellular DNA is a common and unavoidable reaction. The study shows that the thiol residue of glutathione can rapidly add to the 3'-terminus of strand breaks generated from abasic sites. The resulting glutathionylated adduct persists for days in the presence of physiological concentrations of glutathione. The enzyme APE1 can remove the glutathionylated sugar remnant to generate the 3'OH residue required for repair. This work identifies the 3'glutathionylated cleavage product as a major blocking group that needs to be trimmed for efficient repair of abasic site-derived strand breaks.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Suravi Pramanik, Yingling Chen, Heyu Song, Irine Khutsishvili, Luis A. Marky, Sutapa Ray, Amarnath Natarajan, Pankaj K. Singh, Kishor K. Bhakat
Summary: This study identifies a novel role for the protein APE1 in regulating stable G4 formation and KRAS expression in PDAC, suggesting that G4 structures could be potential prognostic markers and therapeutic targets for PDAC.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Anastasiia T. Davletgildeeva, Alexandra A. Kuznetsova, Darya S. Novopashina, Alexander A. Ishchenko, Murat Saparbaev, Olga S. Fedorova, Nikita A. Kuznetsov
Summary: AP-endonucleases are multifunctional enzymes that are required for cell viability. In this study, the effect of metal ions on the exo- and endonuclease activities of four homologous APE1-like endonucleases was analyzed. It was found that these enzymes showed similar dependence patterns on metal ions' concentrations in terms of AP-endonuclease activity. The efficiency of the 3'-5' exonuclease activity was highest in hAPE1, while the endoribonuclease activity varied among the enzymes. These findings indicate that substrate specificity and cleavage efficiency are controlled by factors external to the catalytic site.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Gabriella D. Hartman, Nathan A. Lambert-Cheatham, Mark R. Kelley, Timothy W. Corson
Summary: As global population ages, the prevalence of neovascular eye diseases is increasing, highlighting the importance of developing therapies targeting multiple pathways to alleviate the burden and risks associated with current treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Svetlana Senchurova, Aleksandra A. Kuznetsova, Alexander A. Ishchenko, Murat Saparbaev, Olga S. Fedorova, Nikita A. Kuznetsov
Summary: Escherichia coli apurinic/apyrimidinic (AP) endonuclease Nfo is an important enzyme involved in DNA repair. It recognizes and cleaves AP sites in DNA, which can arise from various sources such as spontaneous hydrolysis or the action of DNA glycosylases. Nfo also has other repair activities, including nucleotide incision repair. This study analyzed the kinetics of Nfo's interaction with DNA substrates and provided insights into the mechanism and rate of nucleotide cleavage. The study also found that 2'-deoxynucleoside monophosphates effectively inhibit Nfo's 3'-5'-exonuclease activity.
Article
Biochemistry & Molecular Biology
Nicole M. Hoitsma, Timothy H. Click, Pratul K. Agarwal, Bret D. Freudenthal
Summary: The study provides insights into the processing of abasic ribonucleotides (rAPs) by APE1, revealing a decrease in activity compared to canonical deoxy-AP substrates. Novel contacts between rAP and the APE1 active site were identified using X-ray crystallography, showing accommodation of the rAP sugar pucker in a C3'-endo conformation. This work advances the understanding of ribonucleotide processing within genomic DNA.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Genetics & Heredity
Millie M. Georgiadis, Qiujia Chen, Jingwei Meng, Chunlu Guo, Randall Wireman, April Reed, Michael R. Vasko, Mark R. Kelley
Article
Oncology
Derek P. Logsdon, Michelle Grimard, Meihua Luo, Safi Shahda, Yanlin Jiang, Yan Tong, Zhangsheng Yu, Nicholas Zyromski, Ernestina Schipani, Fabrizio Carta, Claudiu T. Supuran, Murray Korc, Mircea Ivan, Mark R. Kelley, Melissa L. Fishel
MOLECULAR CANCER THERAPEUTICS
(2016)
Article
Oncology
Jixin Ding, Melissa L. Fishel, April M. Reed, Erin McAdams, Magdalena B. Czader, Angelo A. Cardoso, Mark R. Kelley
MOLECULAR CANCER THERAPEUTICS
(2017)
Article
Oncology
Fenil Shah, Emery Goossens, Nadia M. Atallah, Michelle Grimard, Mark R. Kelley, Melissa L. Fishel
MOLECULAR ONCOLOGY
(2017)
Editorial Material
Cell Biology
Mark R. Kelley, Jill C. Fehrenbacher
NEURAL REGENERATION RESEARCH
(2017)
Article
Neurosciences
Jill C. Fehrenbacher, Chunlu Guo, Mark R. Kelley, Michael R. Vasko
Article
Geriatrics & Gerontology
Tao Yan, Poornima Venkat, Michael Chopp, Alex Zacharek, Peng Yu, Ruizhuo Ning, Xiaoxi Qiao, Mark R. Kelley, Jieli Chen
Article
Pharmacology & Pharmacy
Sheik Pran Babu Sardar Pasha, Kamakshi Sishtla, Rania S. Sulaiman, Bomina Park, Trupti Shetty, Fenil Shah, Melissa L. Fishel, James H. Wikel, Mark R. Kelley, Timothy W. Corson
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2018)
Article
Multidisciplinary Sciences
Derek P. Logsdon, Fenil Shah, Fabrizio Carta, Claudiu T. Supuran, Malgorzata Kamocka, Max H. Jacobsen, George E. Sandusky, Mark R. Kelley, Melissa L. Fishel
SCIENTIFIC REPORTS
(2018)
Article
Cell & Tissue Engineering
Zhigang Cai, Jonathan J. Kotzin, Baskar Ramdas, Sisi Chen, Sai Nelanuthala, Lakshmi Reddy Palam, Ruchi Pandey, Raghuveer Singh Mali, Yan Liu, Mark R. Kelley, George Sandusky, Morvarid Mohseni, Adam Williams, Jorge Henao-Mejia, Reuben Kapur
Article
Chemistry, Medicinal
Richard Trilles, Dmitri Beglov, Qiujia Chen, Hongzhen He, Randall Wireman, April Reed, Spandan Chennamadhavuni, James S. Panek, Lauren E. Brown, Sandor Vajda, John A. Porco, Mark R. Kelley, Millie M. Georgiadis
JOURNAL OF MEDICINAL CHEMISTRY
(2019)
Article
Oncology
Melissa Fishel, Hanyu Xia, Jack McGeown, David W. McIlwain, May Faek Mohamed Elbanna, Ariel A. Craft, Hristos Z. Kaimakliotis, George Sandusky, Chi Zhang, Roberto Pili, Mark R. Kelley, Travis J. Jerde
MOLECULAR CANCER THERAPEUTICS
(2019)
Article
Genetics & Heredity
Marta Codrich, Marina Comelli, Matilde Clarissa Malfatti, Catia Mio, Dilara Ayyildiz, Chi Zhang, Mark R. Kelley, Giovanni Terrosu, Carlo Em Pucillo, Gianluca Tell
Article
Oncology
Charles L. Loprinzi, Christina Lacchetti, Jonathan Bleeker, Guido Cavaletti, Cynthia Chauhan, Daniel L. Hertz, Mark R. Kelley, Antoinette Lavino, Maryam B. Lustberg, Judith A. Paice, Bryan P. Schneider, Ellen M. Lavoie Smith, Mary Lou Smith, Thomas J. Smith, Nina Wagner-Johnston, Dawn L. Hershman
JOURNAL OF CLINICAL ONCOLOGY
(2020)
Article
Pharmacology & Pharmacy
Mark R. Kelley, James H. Wikel, Chunlu Guo, Karen E. Pollok, Barbara J. Bailey, Randy Wireman, Melissa L. Fishel, Michael R. Vasko
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2016)