Article
Cell Biology
Raquel M. Alves-Paiva, Sabrina do Nascimento, Denise De Oliveira, Larissa Coa, Kelen Alvarez, Nelson Hamerschlak, Oswaldo Keith Okamoto, Luciana C. Marti, Andrea T. Kondo, Jose Mauro Kutner, Maria Augusta Tezelli Bortolini, Rodrigo Castro, Juliana A. Preto de Godoy
Summary: This study reports a case where MSCs derived from an elderly donor entered a senescence state after three passages. MSCs cultured with AS displayed an early senescence state, and there were more senescence cells and altered gene expression compared to cells cultured with FBS.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Environmental Sciences
Hana Najahi, Nicola Alessio, Tiziana Squillaro, Gea Oliveri Conti, Margherita Ferrante, Giovanni Di Bernardo, Umberto Galderisi, Imed Messaoudi, Sergio Minucci, Mohamed Banni
Summary: This study evaluated the effects of different size microplastics present in water bottles on human mesenchymal stromal cells. The results showed that microplastic treatments led to a decrease in cell proliferation, altered differentiation potential, and loss of osteocyte phenotype. This indicates that environmental microplastics can be bioavailable for cell uptake and may result in irreversible diseases.
ENVIRONMENTAL RESEARCH
(2022)
Article
Medicine, Research & Experimental
Ji Che, Cuidi Xu, Yuanyuan Wu, Peiyu Jia, Qi Han, Yantao Ma, Xiaolei Wang, Yijie Du, Yongjun Zheng
Summary: This study demonstrated that early-senescent BMSCs accelerate the myogenic differentiation of C2C12 cells, with the transcription factor FOXO3 being the target of the senescent cells. The AKT/P70 signaling pathway mediates the effect of BMSCs on neighboring cells, as seen through the promotion of muscle differentiation by BMSC-derived exosomes.
Article
Endocrinology & Metabolism
Chao Xia, Tianyuan Jiang, Yonghui Wang, Xiaoting Chen, Yan Hu, Yanhong Gao
Summary: The study revealed that p53/miR-145a axis plays a role in promoting senescence and inhibiting osteogenesis of MSCs by targeting core binding factor beta (Cbfb) in osteogenic differentiation. These findings suggest that the p53/miR-145a/Cbfb axis may represent new targets in the treatment of osteoporosis.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Simona Delle Monache, Fanny Pulcini, Francesca Santilli, Stefano Martellucci, Costantino Santacroce, Jessica Fabrizi, Adriano Angelucci, Maurizio Sorice, Vincenzo Mattei
Summary: Studies suggest that hypoxia treatment of DPSCs promotes their differentiation into neuronal cells in a time-dependent manner. Additionally, the conditioned media from hypoxia-stimulated DPSCs can induce neural differentiation in other neural cells.
Article
Health Care Sciences & Services
Suncica Kapor, Milica Vukotic, Tijana Suboticki, Dragoslava Dikic, Olivera Mitrovic Ajtic, Milica Radojkovic, Vladan P. Cokic, Juan F. Santibanez
Summary: The study found that HU inhibits the growth of BMMSC, causing senescence-like changes and affecting cellular differentiation and immunoregulatory functions.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Linshan Xu, Yuyang Wang, Jianping Wang, Jianglong Zhai, Li Ren, Guoying Zhu
Summary: Cellular senescence and its associated secretory phenotype (SASP) are considered potential therapeutic targets for aging-related diseases. Inducing cellular senescence in osteocyte-like cells led to increased expression of senescence and SASP markers, disrupting the differentiation potential of bone marrow mesenchymal stem cells (BMSCs) through paracrine signaling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Yoojin Seo, Min-Jung Kang, Hyung-Sik Kim
Summary: MSCs are used as cell therapeutics for immune disorders due to their immunoregulatory properties, but variation among cells from different donors and rapid clearance after transplantation limit their efficacy. Various bioengineering strategies, including genetic modification and physical engineering, have been suggested to improve the immunomodulatory functions of MSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Jiaming Gao, Hao Wang, Junyan Shen, Xiaojing Liu, Xiaoqi Zhu, Ce Huang, Gongchen Li, Yao Sun, Zhongmin Liu, Yi Eve Sun, Hailiang Liu
Summary: This study reveals the mechanism by which GDF11 reverses aging of mesenchymal stem cells (MSCs) through the activation of the Smad2/3-PI3K-AKT-mTOR signaling pathway and upregulation of the DNA demethylase Tet2. The mutual regulatory relationship between GDF11 and Tet2 plays a crucial role in the anti-aging effects.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Hyoungki Kim, Soonjo Kwon
Summary: The study demonstrates that a hypoxic environment, created using specific chemicals in a suspension culture, enhances the proliferation of mouse clonal mesenchymal stem cells and promotes their differentiation into osteogenic cells.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Cell Biology
Yang Gao, Haoyuan Chen, Xueyu Cang, Hongliang Chen, Yuzhu Di, Jihan Qi, Huimin Cai, Kunpeng Luo, Shizhu Jin
Summary: This study evaluated the therapeutic effect of hair follicle mesenchymal stem cells (HFMSCs) on small intestinal ischemia-reperfusion (IR) injury. Results showed that HFMSCs had the ability to home to damaged sites, differentiate into intestinal stem cells (ISCs) and promote angiogenesis, reduce apoptosis, and regulate oxidative stress response through paracrine mechanism. These findings suggest that HFMSCs can serve as a new treatment option for small intestinal IR injury.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Nicola Alessio, Tiziana Squillaro, Ida Lettiero, Giovanni Galano, Roberto De Rosa, Gianfranco Peluso, Umberto Galderisi, Giovanni Di Bernardo
Summary: Polyphenols, such as piceatannol, show promise in counteracting senescence by reducing the number of senescent cells and promoting the recovery of cell proliferation and stemness properties of MSCs. The multiple mechanisms of piceatannol suggest its potential to combat various senescent phenotypes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Engineering, Biomedical
Jay R. K. Samal, Vignesh K. Rangasami, Sumanta Samanta, Oommen P. Varghese, Oommen P. Oommen
Summary: Studies have shown that culturing MSCs in a low oxygen environment can upregulate stem cell markers and promote cell proliferation in an undifferentiated state. However, there is ambiguity in the results due to differences in oxygen gradients and culture conditions among different studies. This progress report focuses on conflicting findings related to the application of hypoxic conditions for improving MSC proliferation or differentiation, aiming to decipher the factors influencing MSC characteristics under hypoxia.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Biochemistry & Molecular Biology
Mahmoud Al-Azab, Mohammed Safi, Elina Idiiatullina, Fadhl Al-Shaebi, Mohamed Y. Zaky
Summary: Human mesenchymal stem cells (MSCs) play important roles in tissue homeostasis, aging-related diseases, and cellular therapy. Understanding the mechanisms and signs of MSC cellular senescence is crucial for combating aging and cellular senescence. This review discusses the factors contributing to MSC senescence and strategies to reverse it.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2022)
Article
Biotechnology & Applied Microbiology
Mustafa Burak Acar, Serife Ayaz-Guner, Zeynep Gunaydin, Musa Karakukcu, Gianfranco Peluso, Giovanni Di Bernardo, Servet Ozcan, Umberto Galderisi
Summary: Senotherapeutics, including senolytics and senomorphics, can delay replicative senescence and cell death, improving cell function through specific pathways. Metformin, a potential senomorphic agent, may exert its effects by scavenging ROS and regulating key transcription factors involved in blocking oxidative stress.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)