Review
Chemistry, Analytical
Yue Hou, Ruipeng Chen, Zhiguang Wang, Ran Lu, Yonghui Wang, Shuyue Ren, Shuang Li, Yu Wang, Tie Han, Shiping Yang, Huanying Zhou, Zhixian Gao
Summary: Food safety is a major public health challenge, and the development of ultrasensitive detection methods for analytes at ultra-trace levels is crucial. The bio-barcode assay (BCA) has emerged as an effective ultrasensitive detection strategy based on the indirect amplification of DNA probes. This review summarizes the progress of fluorescence, PCR, and colorimetry-based BCA methods for detecting various contaminants in food and discusses current challenges and prospects for BCA in biomedicine and environmental analysis.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Lu-Ming Pan, Xu Zhao, Xiang Wei, Li-Jian Chen, Chan Wang, Xiu-Ping Yan
Summary: A ratiometric luminescence aptasensor based on dual-emissive persistent luminescent nanoparticles (PLNP) was developed for the accurate determination of trace aflatoxin B1 (AFB1) in complex food samples. The aptasensor exhibited excellent linearity, low limit of detection, and good precision. It also showed high selectivity and was successfully applied for the determination of AFB1 in various food samples with satisfactory recoveries.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Benjamin Le Ouay, Ryosuke Minami, Purna K. K. Boruah, Rin Kunitomo, Yuta Ohtsubo, Kohei Torikai, Ryo Ohtani, Cleimence Sicard, Masaaki Ohba
Summary: This study demonstrates the preparation of novel metal-organic polyhedra (MOPs) with anionic or cationic groups, which exhibit high affinity for proteins and can spontaneously form MOP-protein assemblies. The versatility of this method is illustrated using enzymes of different sizes and isoelectric points (pIs). The co-immobilization of cytochrome c with highly charged MOPs leads to a substantial increase in its catalytic activity.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Analytical
Yoonjae Kim, Jongmin Yang, Hyeyeon Hur, Seungju Oh, Hyun Ho Lee
Summary: This study demonstrates the sensitive detection of cortisol using the sandwich assay of cortisol monoclonal antibody and cortisol specific binding aptamer, with a very low detection limit. The use of gold nanoparticles stacking enables highly sensitive colorimetric detection of cortisol on an optically transparent substrate.
Article
Biotechnology & Applied Microbiology
Moumita Dhara, Ashique Al Hoque, Ramkrishna Sen, Debasmita Dutta, Biswajit Mukherjee, Brahamacharry Paul, Soumik Laha
Summary: Hepatocellular carcinoma (HCC) is a leading cause of death globally, and current therapeutics face limitations due to chemotherapeutic resistance and recurrence. Advanced targeted treatment options like immunotherapy, molecular therapy, or surface-engineered nanotherapeutics offer potential benefits in overcoming these limitations. This study focuses on the development of aptamer-conjugated PEGylated nanoliposomes for precise drug delivery to HCC tumors, resulting in improved cancer cell apoptosis and reduced liver toxic effects. The study highlights the therapeutic potential of this alternative drug delivery approach in reducing HCC tumor incidences.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Huijie Han, Jie Chen, Jiachen Li, Alexandra Correia, Raquel Bartolo, Mohammad-Ali Shahbazi, Tambet Teesalu, Shiqi Wang, Wenguo Cui, Helder A. Santos
Summary: Apoptosis is a programmed cell death process responsible for abnormal cell clearance. Cancer cells often develop mechanisms to escape apoptosis, posing a challenge in cancer treatment. A biomimetic lipid nanocarrier that mimics mitochondrial Cytochrome C binding has been developed. This nanocarrier shows great potential in pro-apoptosis cancer therapy, reducing ATP level and promoting apoptosome assembly, leading to higher apoptosis and anti-tumor efficacy.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Pan Gu, Yangfan Lu, Shanni Li, Changbei Ma
Summary: A new label-free fluorescent assay using thioflavin T as a probe has been developed for the detection of trypsin. The assay involves the combination of a specific DNA aptamer with cytochrome c, and trypsin hydrolysis enhances fluorescence intensity. The method exhibited a linear relationship and low detection limit, and was successfully applied to biological samples.
Article
Chemistry, Analytical
Haowei Dong, Deyan Xu, Guanjie Wang, Xiaoya Meng, Xia Sun, Qingqing Yang, Yemin Guo, Yelong Zhu
Summary: The massive use of organophosphorus pesticides poses a great threat to food safety, human health, and environmental protection. In this paper, a time-resolved fluorescent immunochromatographic strip is proposed for the simultaneous detection of multiple organophosphorus pesticides. The method has advantages such as signal amplification and easy operation, and performs well in the testing of vegetable samples.
ANALYTICAL METHODS
(2022)
Article
Agriculture, Multidisciplinary
Bo Zou, Qianqian Yu, Lele Shao, Yingying Sun, Xingmin Li, Ruitong Dai
Summary: Lipid molecules play a crucial role in mitochondria-mediated apoptosis. This study reveals that the levels of mitochondrial lipids increase during early postmortem, accelerating apoptosis in beef muscle. The psoas major muscle has a higher degree of apoptosis and more severe muscle oxidation and antioxidant system damage compared to the longissimus lumborum muscle.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Cristian Silviu Moldovan, Anca Onaciu, Valentin Toma, Radu Marginean, Alin Moldovan, Adrian Bogdan Tigu, Gabriela Fabiola Stiufiuc, Constantin Mihai Lucaciu, Rares Ionut Stiufiuc
Summary: A method using carbon quantum dots smaller than 5 nm synthesized from sodium citrate and polyethylene imine was successfully employed to detect the release of Cyt c. Significant drops in fluorescence intensity were observed in cells treated with apoptosis-inducing therapeutic compounds, confirming the release of Cyt c from mitochondria to cytosol. This method could potentially contribute to an indirect in vitro evaluation of apoptosis.
Article
Biochemistry & Molecular Biology
Rony Abdi Syahputra, Urip Harahap, Yahdiana Harahap, Andayana Puspitasari Gani, Aminah Dalimunthe, Amer Ahmed, Satirah Zainalabidin
Summary: This study aimed to analyze the effects of doxorubicin on cardiac histology and investigate the protective effect of compounds from Vernonia amygdalina against cardiac toxicity. The results showed that compounds from Vernonia amygdalina could protect the heart from doxorubicin toxicity by reducing the expression of TGF, cytochrome c, and apoptosis.
Article
Medicine, Research & Experimental
Kai-Yue Cao, Tong-Meng Yan, Ji-Zhou Zhang, Ting-Fung Chan, Jie Li, Chong Li, Elaine Lai-Han Leung, Jin Gao, Bao-Xian Zhang, Zhi-Hong Jiang
Summary: This study reveals a new role of plant-derived tRFs in regulating endogenous genes and provides insights into the discovery of unknown molecular targets for therapeutics.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Chemistry, Multidisciplinary
Zhixiang Li, Xueyan Feng, Wenping Hu, Lele Li
Summary: A novel activatable DNA nanodevice was designed for real-time imaging of apoptosis-related dual proteins. The probe has turn-on and specific recognition properties, allowing for spatially selective detection of apoptotic-related markers in living cells.
Article
Nanoscience & Nanotechnology
Weina Zhang, Pu Liu, Guowei Yang, Hongxiang Lei
Summary: Nanowires fabricated from polylactic acid (PLA) using a direct drawing method exhibit high optical transparency, good biocompatibility, biodegradability, and flexibility. The nanowires achieved ultra-low concentration sensing of cytochrome c with a detection limit of 1.38 x 10(-17) M, much lower than other semiconductor/metal-based nanosensors. Additionally, they enable label-free, real-time monitoring of cell apoptosis and pH sensing through quantum dot doping. These results highlight the potential of PLA nanowires in multifunctional biosensing and biodetection, advancing the field of photomedicine.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Sarita Yadav, Neha Sawarni, Preeti Kumari, Minakshi Sharma
Summary: Cytochrome c plays a crucial role in the mitochondrial electron transport chain and can be used as a biomarker to measure mitochondrial damage and various diseases. The level of cytochrome c in the bloodstream is associated with cellular diseases and can be used to predict disease progression by analyzing serum samples.
PROCESS BIOCHEMISTRY
(2022)
Article
Biophysics
Hengji Cong, Fong-Chuen Loo, Jiajie Chen, Yuye Wang, Siu-Kai Kong, Ho-Pui Ho
BIOSENSORS & BIOELECTRONICS
(2019)
Article
Chemistry, Applied
Jacky Fong-Chuen Loo, Grace Wing-Chiu But, Ho-Chin Kwok, Pui-Man Lau, Siu-Kai Kong, Ho-Pui Ho, Pang-Chui Shaw
Review
Chemistry, Analytical
Dongping Wang, Jacky Fong Chuen Loo, Jiajie Chen, Yeung Yam, Shih-Chi Chen, Hao He, Siu Kai Kong, Ho Pui Ho
Article
Biochemical Research Methods
Jianing Zhang, Yue Ma, Kuan Hu, Yuan Feng, Si Chen, Xiaoyang Yang, Jacky Fong-Chuen Loo, Han Zhang, Feng Yin, Zigang Li
BIOCONJUGATE CHEMISTRY
(2019)
Article
Infectious Diseases
Lang Li, Jian-an He, Wei Wang, Yun Xia, Li Song, Ze-han Chen, Hang-zhi Zuo, Xuan-Ping Tan, Aaron Ho-Pui Ho, Siu-Kai Kong, Jacky Fong-Chuen Loo, Hua-wen Li, Gu Dayong
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2019)
Review
Materials Science, Multidisciplinary
Jiajie Chen, Jacky Fong-Chuen Loo, Dongping Wang, Yu Zhang, Siu-Kai Kong, Ho-Pui Ho
ADVANCED OPTICAL MATERIALS
(2020)
Article
Medicine, Research & Experimental
Wei Wang, Rui-ling Dong, Dayong Gu, Jian-an He, Pin Yi, Siu-Kai Kong, Ho-Pui Ho, Jacky Fong-Chuen Loo, Wen Wang, Qian Wang
ADVANCES IN MEDICAL SCIENCES
(2020)
Review
Chemistry, Inorganic & Nuclear
Jacky Fong-Chuen Loo, Yi-Hsin Chien, Feng Yin, Siu-Kai Kong, Ho-Pui Ho, Ken-Tye Yong
COORDINATION CHEMISTRY REVIEWS
(2019)
Article
Chemistry, Analytical
Dongping Wang, Jacky Fong Chuen Loo, Wei Lin, Qiang Geng, Erika Kit Shan Ngan, Siu Kai Kong, Yeung Yam, Shih-Chi Chen, Ho Pui Ho
SENSORS AND ACTUATORS B-CHEMICAL
(2020)
Article
Chemistry, Multidisciplinary
Joonas Ryssy, Arttu J. Lehtonen, Jacky Loo, Minh-Kha Nguyen, Jani Seitsonen, Yike Huang, Badri Narayanan Narasimhan, Juho Pokki, Anton Kuzyk, Sesha Manuguri
Summary: This work showcases a light-mediated approach to tune the orientational features of gold nanorods in DNA-engineered hydrogel materials. The strategy relies on the use of visible-light-induced photothermal effects to cause deformation of the hydrogel matrix, resulting in temperature-controlled polarization-dependent optical responses. This approach can open novel avenues to create dynamic light-responsive materials with reconfigurable plasmonic responses.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Yike Huang, Minh-Kha Nguyen, Vu Hoang Nguyen, Jacky Loo, Arttu J. Lehtonen, Anton Kuzyk
Summary: This study presents a strategy to characterize aptamers using DNA origami-based chiral plasmonic assemblies as reporters and establishing a competitive hybridization reaction-based thermodynamic model. The characterization scheme demonstrated reliable and reproducible characterization of various aptamers, facilitating the development of aptamer-based applications.
Review
Biochemical Research Methods
Sesha Manuguri, Minh-Kha Nguyen, Jacky Loo, Ashwin Karthick Natarajan, Anton Kuzyk
Summary: The DNA origami technique discovered in 2006 has revolutionized bottom-up nanofabrication, enabling fabrication of nanostructures of almost any shape and serving as templates for various nanoscale components. However, its applicability is often restricted by limited stability in specific conditions.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Minghui Ji, Yun Xia, Jacky Fong-Chuen Loo, Lang Li, Ho-Pui Ho, Jianan He, Dayong Gu
Article
Music
Fung Chiat Loo, Fung Ying Loo, Yan Piaw Chua
Review
Materials Science, Biomaterials
Jacky Fong-Chuen Loo, Ho Pui Ho, Siu Kai Kong, Tza-Huei Wang, Yi-Ping Ho
ADVANCED BIOSYSTEMS
(2019)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)