Article
Optics
Jiajie Chen, Zhi Chen, Changle Meng, Jianxing Zhou, Yuhang Peng, Xiaoqi Dai, Jingfeng Li, Yili Zhong, Xiaolin Chen, Wu Yuan, Ho-Pui Ho, Bruce Zhi Gao, Junle Qu, Xueji Zhang, Han Zhang, Yonghong Shao
Summary: Optothermal nanotweezers have been developed as an innovative optical manipulation technique that combines optothermal manipulation and CRISPR-based bio-detection. This innovative approach endows optical tweezers with the ability to identify DNA in aqueous solutions.
LIGHT-SCIENCE & APPLICATIONS
(2023)
Article
Biochemical Research Methods
Huichao Chai, Yongxiang Feng, Fei Liang, Wenhui Wang
Summary: In this paper, a novel microfluidic device with high-efficiency cell trapping and deterministic release is presented. The device includes capillary valves that can open in a predefined pattern to sequentially trap incoming cells into separate sites. Experiments using MCF-7 cells demonstrated the capability of the device for deterministic single cell trapping and release, showing promise as a useful platform for single cell analysis.
Article
Biochemical Research Methods
Lei Zhang, Moonsook Lee, Alexander Y. Maslov, Cristina Montagna, Jan Vijg, Xiao Dong
Summary: This study presents a detailed protocol for single-cell whole-genome sequencing to discover and analyze somatic mutations in tissues and organs. The protocol includes steps such as single-cell isolation, whole-genome amplification, library preparation, and sequencing, as well as computational analyses. It provides high genomic coverage and accuracy compared to other single-cell amplification methods.
Article
Quantum Science & Technology
T-W Hsu, W. Zhu, T. Thiele, M. O. Brown, S. B. Papp, A. Agrawal, C. A. Regal
Summary: In this study, we demonstrate the use of efficient dielectric metasurface lens for trapping and imaging single neutral atoms. We compare the performance of the metasurface lens with numerical computations and predict its potential for future applications in atom trapping and quantum information experiments.
Article
Biochemical Research Methods
Bo Yang, Ping Wang, Zhenqing Li, Qingxiang You, Shinichi Sekine, Junshan Ma, Songlin Zhuang, Dawei Zhang, Yoshinori Yamaguchi
Summary: A multiplex circular array shaped CF-PCR microfluidic chip was designed for on-site detection of bacteria. The chip consisted of 12 serpentine microchannels distributed on a disc, with each microchannel having an inlet and an outlet for sample injection and detection of PCR products based on fluorescence. By using a one-to-twelve diverter, samples could be simultaneously driven into each inlet. The system achieved simultaneous amplification of 12 different target genes and on-site detection of their corresponding positives within 23 minutes.
Article
Multidisciplinary Sciences
Tomoki Murakami, Hiroto Teratani, Dai'ichiro Aoki, Masao Noguchi, Mamiko Tsugane, Hiroaki Suzuki
Summary: In this study, a hydrodynamic cell-trapping system in an open microfluidics architecture was developed, allowing external access to trapped cells and enabling automated retrieval. This system bridges the gap between microfluidic devices and conventional macro-scale apparatus used in biology, fostering the development of new single-cell analytical systems.
Article
Multidisciplinary Sciences
Gerda Kildisiute, Waleed M. Kholosy, Matthew D. Young, Kenny Roberts, Rasa Elmentaite, Sander R. van Hooff, Clarissa N. Pacyna, Eleonora Khabirova, Alice Piapi, Christine Thevanesan, Eva Bugallo-Blanco, Christina Burke, Lira Mamanova, Kaylee M. Keller, Karin P. S. Langenberg-Ververgaert, Philip Lijnzaad, Thanasis Margaritis, Frank C. P. Holstege, Michelle L. Tas, Marc H. W. A. Wijnen, Max M. van Noesel, Ignacio Del Valle, Giuseppe Barone, Reinier van der Linden, Catriona Duncan, John Anderson, John C. Achermann, Muzlifah Haniffa, Sarah A. Teichmann, Dyanne Rampling, Neil J. Sebire, Xiaoling He, Ronald R. de Krijger, Roger A. Barker, Kerstin B. Meyer, Omer Bayraktar, Karin Straathof, Jan J. Molenaar, Sam Behjati
Summary: The neuroblastoma cancer cells resemble fetal sympathoblasts, indicating a pan-neuroblastoma cancer cell state that could potentially be targeted for novel treatment strategies. The findings highlight the importance of understanding the phenotype of neuroblastoma cells for the development of immunotherapeutic and targeted avenues.
Article
Biophysics
Chunhuan Xu, Jingjin Zhao, Shengyu Chen, Ivan Yu Sakharov, Shengqiang Hu, Shulin Zhao
Summary: A novel DNA machine-based approach for absolute quantification of mRNA in individual cells is presented. This method provides important insights into cellular function and has great research and diagnostic value.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemical Research Methods
Tomoko Yoshino, Yihao Mao, Yoshiaki Maeda, Ryo Negishi, Satoshi Murata, Seiichiro Moriya, Hirofumi Shimada, Atsushi Arakaki, Kenichi Kobayashi, Yoshitsugu Hagiwara, Kazutoshi Okamoto, Tsuyoshi Tanaka
Summary: Researchers have developed a technology called MCA/GCM that can directly analyze phytoplankton in marine environments without the need for cumbersome pretreatment, providing a powerful tool for studying phytoplankton diversity.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Kaile Wang, Tapsi Kumar, Junke Wang, Darlan Conterno Minussi, Emi Sei, Jianzhuo Li, Tuan M. Tran, Aatish Thennavan, Min Hu, Anna K. Casasent, Zhenna Xiao, Shanshan Bai, Lei Yang, Lorraine M. King, Vandna Shah, Petra Kristel, Carolien L. van der Borden, Jeffrey R. Marks, Yuehui Zhao, Amado J. Zurita, Ana Aparicio, Brian Chapin, Jie Ye, Jianjun Zhang, Ellinor Grand Challenge PRECISION Consortium, Ellinor Sawyer, Alastair M. Thompson, Andrew Futreal, E. Shelley Hwang, Jelle Wesseling, Esther H. Lips, Nicholas E. Navin
Summary: Ductal carcinoma in situ (DCIS) is a precursor of invasive breast cancer, and there is limited understanding of its genomic progression to recurrent disease, mainly due to challenges in genomic profiling of FFPE materials. In this study, a high-throughput single-cell DNA-sequencing method called Arc-well was developed and validated with FFPE samples. The results revealed that DCIS had undergone whole-genome doubling and clonal diversification, sharing genomic lineages with persistent subclones in recurrences.
Article
Biotechnology & Applied Microbiology
Jia Zhang, Xiaolu Su, Yefei Wang, Xiaohang Wang, Shiqi Zhou, Hui Jia, Xiaoyan Jing, Yanhai Gong, Jichao Wang, Jian Xu
Summary: Improved Single-cell Genome Amplification (iSGA) overcomes the low amplification efficiency, incomplete and uneven genome amplification issues in single-cell sequencing workflows through phi29 DNA polymerase engineering and process engineering. The developed HotJa Phi29 DNA Polymerase shows significantly better coverage (99.75%) at higher temperatures and achieves high single-cell genome amplification ability and coverage (93.59%) for commercial probiotic samples. iSGA is more efficient and cost-effective than the commercial Thermo Scientific EquiPhi29 DNA Polymerase, promising its broad applications in large-scale single-cell sequencing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Physical
Christine A. Carlson, Xavier S. Udad, Quintus Owen, Alaknanda P. Amin-Patel, Woo-Jin Chang, Jorg C. Woehl
Summary: This study reports the reversible and tunable trapping of single DNA molecules and other charged microparticles and nanoparticles in aqueous solution using a DC corral trap setup. The trap shows the ability to stably confine charged objects at the nanoscale, control trap stiffness with applied voltage, and simultaneously trap multiple objects.
JOURNAL OF CHEMICAL PHYSICS
(2022)
Article
Multidisciplinary Sciences
David Laehnemann, Johannes Koester, Ute Fischer, Arndt Borkhardt, Alice C. McHardy, Alexander Schoenhuth
Summary: ProSolo is a tool for calling single nucleotide variants from MDA single cell DNA sequencing data, which integrates all relevant MDA biases to achieve higher accuracy and reliability in single cells compared to existing tools.
NATURE COMMUNICATIONS
(2021)
Article
Nanoscience & Nanotechnology
Deepika Sharma, Roderick Y. H. Lim, Thomas Pfohl, Yasin Ekinci
Summary: The focus of this work is on the development of simple and effective nanofluidic devices for trapping single charged nanoparticles in an aqueous environment. By modifying the surface of the device to change its charge properties and reducing the surface modification time, the study demonstrates the successful enhancement in device performance.
MICROSYSTEMS & NANOENGINEERING
(2021)
Review
Biochemical Research Methods
Julie Van Lent, Jolien Breukers, Karen Ven, Louanne Ampofo, Sara Horta, Francesca Pollet, Maya Imbrechts, Nick Geukens, Karen Vanhoorelbeke, Paul Declerck, Jeroen Lammertyn
Summary: Antibodies are essential biologicals with high therapeutic and diagnostic value, and fully-human monoclonal antibodies are thriving in the global therapeutic market. The rapidly emerging single-cell technologies have enabled efficient discovery and production of monoclonal antibodies by screening B cells. The key challenges and principles of discovering monoclonal antibodies using single-cell technologies involve confinement of single cells, identification of cells of interest, retrieval of these cells, and single-cell sequencing for production.
Article
Infectious Diseases
Lang Li, Jian-an He, Wei Wang, Yun Xia, Li Song, Ze-han Chen, Hang-zhi Zuo, Xuan-Ping Tan, Aaron Ho-Pui Ho, Siu-Kai Kong, Jacky Fong-Chuen Loo, Hua-wen Li, Gu Dayong
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2019)
Review
Materials Science, Multidisciplinary
Jiajie Chen, Jacky Fong-Chuen Loo, Dongping Wang, Yu Zhang, Siu-Kai Kong, Ho-Pui Ho
ADVANCED OPTICAL MATERIALS
(2020)
Article
Medicine, Research & Experimental
Wei Wang, Rui-ling Dong, Dayong Gu, Jian-an He, Pin Yi, Siu-Kai Kong, Ho-Pui Ho, Jacky Fong-Chuen Loo, Wen Wang, Qian Wang
ADVANCES IN MEDICAL SCIENCES
(2020)
Article
Chemistry, Analytical
Dongping Wang, Jacky Fong Chuen Loo, Wei Lin, Qiang Geng, Erika Kit Shan Ngan, Siu Kai Kong, Yeung Yam, Shih-Chi Chen, Ho Pui Ho
SENSORS AND ACTUATORS B-CHEMICAL
(2020)
Review
Optics
Yuye Wang, Shuwen Zeng, Georges Humbert, Ho-Pui Ho
LASER & PHOTONICS REVIEWS
(2020)
Article
Nanoscience & Nanotechnology
Yuye Wang, Shuwen Zeng, Aurelian Crunteanu, Zhenming Xie, Georges Humbert, Libo Ma, Yuanyuan Wei, Aude Brunel, Barbara Bessette, Jean-Christophe Orlianges, Fabrice Lalloue, Oliver G. Schmidt, Nanfang Yu, Ho-Pui Ho
Summary: An enhanced plasmonic biosensor was developed in this study to detect small cancer biomarkers with low molecular weight and low concentration range. By precisely engineering atomically thin materials, a phase singularity and significant lateral position shift effect were achieved, enabling the detection of TNF-alpha cancer marker at the femtomolar level.
NANO-MICRO LETTERS
(2021)
Article
Chemistry, Multidisciplinary
Joonas Ryssy, Arttu J. Lehtonen, Jacky Loo, Minh-Kha Nguyen, Jani Seitsonen, Yike Huang, Badri Narayanan Narasimhan, Juho Pokki, Anton Kuzyk, Sesha Manuguri
Summary: This work showcases a light-mediated approach to tune the orientational features of gold nanorods in DNA-engineered hydrogel materials. The strategy relies on the use of visible-light-induced photothermal effects to cause deformation of the hydrogel matrix, resulting in temperature-controlled polarization-dependent optical responses. This approach can open novel avenues to create dynamic light-responsive materials with reconfigurable plasmonic responses.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Yike Huang, Minh-Kha Nguyen, Vu Hoang Nguyen, Jacky Loo, Arttu J. Lehtonen, Anton Kuzyk
Summary: This study presents a strategy to characterize aptamers using DNA origami-based chiral plasmonic assemblies as reporters and establishing a competitive hybridization reaction-based thermodynamic model. The characterization scheme demonstrated reliable and reproducible characterization of various aptamers, facilitating the development of aptamer-based applications.
Review
Biochemical Research Methods
Sesha Manuguri, Minh-Kha Nguyen, Jacky Loo, Ashwin Karthick Natarajan, Anton Kuzyk
Summary: The DNA origami technique discovered in 2006 has revolutionized bottom-up nanofabrication, enabling fabrication of nanostructures of almost any shape and serving as templates for various nanoscale components. However, its applicability is often restricted by limited stability in specific conditions.
BIOCONJUGATE CHEMISTRY
(2022)
Proceedings Paper
Engineering, Electrical & Electronic
Shuwen Zeng, Yuye Wang, Aurelian Crunteanu, Georges Humbert, Jean-Christophe Orlianges, Ho-Pui Ho
2020 OPTO-ELECTRONICS AND COMMUNICATIONS CONFERENCE (OECC 2020)
(2020)
Proceedings Paper
Engineering, Electrical & Electronic
Yuye Wang, Shuwen Zeng, Aurelian Crunteanu, Yuanyuan Wei, Georges Humbert, Jean-Christophe Orlianges, Ho-Pui Ho
2020 CONFERENCE ON LASERS AND ELECTRO-OPTICS (CLEO)
(2020)
Article
Chemistry, Multidisciplinary
Minghui Ji, Yun Xia, Jacky Fong-Chuen Loo, Lang Li, Ho-Pui Ho, Jianan He, Dayong Gu
Article
Music
Fung Chiat Loo, Fung Ying Loo, Yan Piaw Chua
Article
Biochemical Research Methods
Yuye Wang, Shiyue Liu, Tiankai Zhang, Hengji Cong, Yuanyuan Wei, Jianbin Xu, Yi-Ping Ho, Siu-Kai Kong, Ho-Pui Ho
Review
Materials Science, Biomaterials
Jacky Fong-Chuen Loo, Ho Pui Ho, Siu Kai Kong, Tza-Huei Wang, Yi-Ping Ho
ADVANCED BIOSYSTEMS
(2019)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)