4.5 Article

Effects of myo-inositol on proliferation, differentiation, oxidative status and antioxidant capacity of carp enterocytes in primary culture

期刊

AQUACULTURE NUTRITION
卷 19, 期 1, 页码 45-53

出版社

WILEY-HINDAWI
DOI: 10.1111/j.1365-2095.2011.00934.x

关键词

antioxidant; enterocyte; growth; intestine; Jian carp (Cyprinus carpio var; Jian); myo-inositol

资金

  1. National Science Foundation of China [31001116]
  2. Program for New Century Excellent Talents in University of Ministry of Education of China [NCET-08-0905]
  3. National Department Public Benefit Research Foundation (Agriculture) of China [2010003020]
  4. Scientific Research Foundations of the Education Department of Sichuan Province, China [09zz026]

向作者/读者索取更多资源

This study investigated the effects of myo-inositol (MI) on the growth and antioxidant capacity of carp enterocytes. The enterocytes were incubated in media containing 0, 15, 30, 45, 60 and 75 mg MI L-1 for 96 h. The results indicated that MI could increase cell viability. In addition, the activities of cellular alkaline phosphatase (AKP), gamma-glutamyl transpeptidase (?-GT), Na+, K+-adenosine trisphosphatase (Na+, K+-ATPase) and creatinkinase (CK) increased with MI supplementation at levels ranging from 15 to 60 mg MI L-1 medium, indicating an improvement in cell differentiation and function. Further, enzymatic antioxidant ability, as measured by total superoxide dismutase (T-SOD), Cu/Zn-SOD, Mn-SOD, catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) activities, improved with MI supplementation. Finally, cell damage, as indicated by lactic acid dehydrogenase (LDH) activity, malondialdehyde (MDA) content of the medium and cellular protein carbonyls (PC), was all depressed by MI. Correlation analyses showed that cell viability (MTT) was positively related to the antioxidant enzyme activities, but negatively related to cell damage (LDH, MDA and PC). In summary, the data showed that MI could improve the growth of fish enterocytes. This result may be partly due to the enhanced antioxidant status and depressed oxidative damage.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.5
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据