Coevolution of both Thermostability and Activity of Polyphosphate Glucokinase from Thermobifida fusca YX

Ultra-rapid rates of water splitting for biohydrogen gas production through in vitro artificial enzymatic pathways

(2018) Eui-Jin Kim et al.   Energy & Environmental Science

Production of myo-inositol from glucose by a novel trienzymatic cascade of polyphosphate glucokinase, inositol 1-phosphate synthase and inositol monophosphatase

(2018) Yiping Lu et al.   ENZYME AND MICROBIAL TECHNOLOGY

An appraisal of the enzyme stability-activity trade-off

(2017) Scott R. Miller   EVOLUTION

A root penetration model of Arabidopsis thaliana in phytagel medium with different strength

(2017) Jie Yan et al.   JOURNAL OF PLANT RESEARCH

In vitro metabolic engineering of bioelectricity generation by the complete oxidation of glucose

(2017) Zhiguang Zhu et al.   METABOLIC ENGINEERING

Biosynthesis of plant-derived ginsenoside Rh2 in yeast via repurposing a key promiscuous microbial enzyme

(2017) Yu Zhuang et al.   METABOLIC ENGINEERING

ATP-free biosynthesis of a high-energy phosphate metabolite fructose 1,6-diphosphate by in vitro metabolic engineering

(2017) Wei Wang et al.   METABOLIC ENGINEERING

Biocatalytic Phosphorylations of Metabolites: Past, Present, and Future

(2017) Roland Wohlgemuth et al.   TRENDS IN BIOTECHNOLOGY

Thermal Cycling Cascade Biocatalysis of myo-Inositol Synthesis from Sucrose

(2017) Chao Zhong et al.   ACS Catalysis

Facile Construction of Random Gene Mutagenesis Library for Directed Evolution Without the Use of Restriction Enzyme inEscherichia coli

(2016) Jae-Eung Kim et al.   Biotechnology Journal

Defying the activity–stability trade-off in enzymes: taking advantage of entropy to enhance activity and thermostability

(2016) Khawar Sohail Siddiqui   CRITICAL REVIEWS IN BIOTECHNOLOGY

Consensus protein design

(2016) Benjamin T. Porebski et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Evolutionary drivers of thermoadaptation in enzyme catalysis

(2016) Vy Nguyen et al.   SCIENCE

Versatile Peptide C-Terminal Functionalization via a Computationally Engineered Peptide Amidase

(2016) Bian Wu et al.   ACS Catalysis

Facile Construction of Random Gene Mutagenesis Library for Directed Evolution Without the Use of Restriction Enzyme inEscherichia coli

(2016) Jae-Eung Kim et al.   Biotechnology Journal

A general and efficient strategy for generating the stable enzymes

(2016) Xiao-Fei Zhang et al.   Scientific Reports

High-Throughput Screening of Coenzyme Preference Change of Thermophilic 6-Phosphogluconate Dehydrogenase from NADP+ to NAD+

(2016) Rui Huang et al.   Scientific Reports

Emerging Enzymes for ATP Regeneration in Biocatalytic Processes

(2015) Jennifer N. Andexer et al.   CHEMBIOCHEM

High-yield hydrogen production from biomass by in vitro metabolic engineering: Mixed sugars coutilization and kinetic modeling

(2015) Joseph A. Rollin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Two strictly polyphosphate-dependent gluco(manno)kinases from diazotrophic Cyanobacteria with potential to phosphorylate hexoses from polyphosphates

(2014) Tomás Albi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Enhanced Enzyme Kinetic Stability by Increasing Rigidity within the Active Site

(2014) Yuan Xie et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

All1371 is a polyphosphate-dependent glucokinase in Anabaena sp. PCC 7120

(2014) F. Klemke et al.   MICROBIOLOGY-SGM

Engineering of an epoxide hydrolase for efficient bioresolution of bulky pharmaco substrates

(2014) X.-D. Kong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Computationally designed libraries for rapid enzyme stabilization

(2014) H. J. Wijma et al.   PROTEIN ENGINEERING DESIGN & SELECTION

A high-energy-density sugar biobattery based on a synthetic enzymatic pathway

(2014) Zhiguang Zhu et al.   Nature Communications

Reporter-based screening and selection of enzymes

(2013) Teunke van Rossum et al.   FEBS Journal

Engineering the third wave of biocatalysis

(2012) U. T. Bornscheuer et al.   NATURE

Highly thermostable fungal cellobiohydrolase I (Cel7A) engineered using predictive methods

(2012) Russell S. Komor et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Simple Cloning via Direct Transformation of PCR Product (DNA Multimer) to Escherichia coli and Bacillus subtilis

(2011) Chun You et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

One-step purification and immobilization of thermophilic polyphosphate glucokinase from Thermobifida fusca YX: glucose-6-phosphate generation without ATP

(2011) Hehuan Liao et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Cg2091 encodes a polyphosphate/ATP-dependent glucokinase of Corynebacterium glutamicum

(2010) Steffen N. Lindner et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Thermostability Enhancement of Clostridium thermocellum Cellulosomal Endoglucanase Cel8A by a Single Glycine Substitution

(2010) Michael Anbar et al.   ChemCatChem

Protein stability and enzyme activity at extreme biological temperatures

(2010) Georges Feller   JOURNAL OF PHYSICS-CONDENSED MATTER

Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution

(2009) Eugene Rha et al.   FEBS Journal

Directed evolution drives the next generation of biocatalysts

(2009) Nicholas J Turner   Nature Chemical Biology

Exploring protein fitness landscapes by directed evolution

(2009) Philip A. Romero et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Shuttle Vector Expression in Thermococcus kodakaraensis: Contributions of cis Elements to Protein Synthesis in a Hyperthermophilic Archaeon

(2008) T. J. Santangelo et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Thermostable Bacillus subtilis Lipases: In Vitro Evolution and Structural Insight

(2008) Shoeb Ahmad et al.   JOURNAL OF MOLECULAR BIOLOGY