Article
Food Science & Technology
Xin Shen, Weicheng Li, Hongyu Cai, Shuai Guo, Min Li, Yangshuo Liu, Zhihong Sun
Summary: This study analyzed the fermentation capacity and metabolic profiles of 17 L. lactis subsp. lactis strains and found significant metabolic differences between strains with different fermentation rates. Peptides, esters, and tributyrin were identified as biomarkers to distinguish between fast and slow fermentation groups.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Food Science & Technology
Danielle Nader Furtado, Bernadette Dora Gombossy de Melo Franco, Svetoslav Dimitrov Todorov
Summary: Differentiated Lactococcus lactis bacteriocin producers' strains using repPCR technique. The stability of bacteriocin was found to be influenced by applied chemicals, pH, and temperature. The addition of bacteriocin completely inhibited the growth of Listeria monocytogenes ScottA, Lactobacillus sakei ATCC 15521, or Enterococcus faecium ATCC 19434 in the study.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Begona Redruello, Yasmine Saidi, Lorena Sampedro, Victor Ladero, Beatriz del Rio, Miguel A. Alvarez
Summary: This study isolated six GABA-producing Lactococcus lactis strains from camel's milk for the first time and confirmed their potential as starter cultures for producing GABA-enriched cheeses. Experimental cheeses made with these strains accumulated GABA at concentrations of up to 457 mg/kg, suggesting they could offer health benefits to consumers.
Letter
Microbiology
Vera Kuzina Poulsen, Elahe Ghanei Moghadam, Stjepan Kresimir Kracun, Birgit Albrecht Svendsen, Wioleta Marta Nielsen, Gunnar Oregaard, Anders Krarup
Summary: By high-throughput screening of 1232 Lactococcus lactis strains, it was found that most strains with texturing abilities in fermented milk were also capable of enhancing the texture in fermented soybean matrices. The production of exocellular polysaccharides is believed to contribute positively to the improved texture in both milk and plant-based fermentation.
FEMS MICROBIOLOGY LETTERS
(2022)
Article
Biotechnology & Applied Microbiology
Gee Hyeun Choi, Joanna Ivy Irorita Fugaban, Clarizza May Dioso, Jorge Enrique Vazquez Bucheli, Wilhelm Heinrich Holzapfel, Svetoslav Dimitrov Todorov
Summary: Bacteriocins are antimicrobial peptides produced by bacteria, including lactic acid bacteria. They have broad spectra of inhibition and potential applications in food preservation and pharmaceutical sector. In this study, several promising bacteriocin-producing strains were isolated and evaluated for their stability and adherence properties. The bacteriocins produced by these strains showed antimicrobial effects against Listeria spp. and Staphylococcus spp. The presence of gene mutations that may affect bacteriocin expression was also observed.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Biotechnology & Applied Microbiology
Adilene Mendoza-Salazar, Lourdes Santiago-Lopez, Maria J. Torres-Llanez, Adrian Hernandez-Mendoza, Belinda Vallejo-Cordoba, Andrea M. Liceaga, Aaron F. Gonzalez-Cordova
Summary: This study evaluated the antioxidant and ACEI activity of edible insect flours fermented with specific Lactococcus lactis strains. The results showed that fermented grasshopper flours demonstrated higher antioxidant activity and ACEI inhibition compared to mealworm flours. Fermentation increased protein hydrolysis, total polyphenol content, and peptide profile, providing potential bioactive compounds for antioxidant and antihypertensive purposes.
FERMENTATION-BASEL
(2021)
Article
Multidisciplinary Sciences
Xuan Zou, Xiaohong Xiao, Ziran Mo, Yashi Ge, Xing Jiang, Ruolin Huang, Mengxue Li, Zixin Deng, Shi Chen, Lianrong Wang, Sang Yup Lee
Summary: In this study, E. coli host strains with broad antiphase activities were constructed by integrating the Ssp defense system into the genome and introducing mutations in key components essential for phage infection cycles. These engineered E. coli strains showed strong resistance against various phages without affecting cell growth and maintained the production capabilities of recombinant proteins even under high levels of phage challenge.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Javier Rodriguez, Lucia Vazquez, Ana Belen Florez, Baltasar Mayo
Summary: This study reports the characterization of three lactic acid bacteria strains existing as a stable consortium in naturally fermented milk. The strains were analyzed for phenotyping, genetic properties, and interaction during milk fermentation. The study showed phenotypic diversity among the strains and identified potential uses in dairy fermentations.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Frank Oechslin, Carmen Menzi, Philippe Moreillon, Gregory Resch
Summary: This study explored the roles of different domains in the PlySK1249 endolysin and how they synergize for bacterial lysis. The Ami and CHAP domains work together to digest peptidoglycan efficiently, while proteolytic cleavage by host cell wall proteases may modulate the enzyme's lytic activity. Understanding the multidomain interplay of endolysins like PlySK1249 can contribute to the development of more effective therapeutic antibacterial agents.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Javier Feito, Carlos Araujo, Sara Arbulu, Diogo Contente, Beatriz Gomez-Sala, Lara Diaz-Formoso, Estefania Munoz-Atienza, Juan Borrero, Luis M. Cintas, Pablo E. Hernandez
Summary: This study reported the design of Lactococcus lactis strains that can produce bacteriocins GarA and/or GarQ, along with NisA or NisZ. The strains with GarA, GarQ, and NisZ demonstrated the highest antimicrobial activity against virulent L. garvieae strains.
Article
Microbiology
Juliana Lima Alves, Luisa Lemos, Nubia Morais Rodrigues, Vanessa Bastos Pereira, Patricia A. Vieira Barros, Maria Cecilia Campos Canesso, Mauro A. F. Guimaraes, Denise Carmona Cara, Anderson Miyoshi, Vasco Ariston Azevedo, Tatiani Uceli Maioli, Ana Cristina Gomes-Santos, Ana Maria Caetano Faria
Summary: Inflammatory bowel diseases (IBD) are gastrointestinal disorders characterized by a breakdown in intestinal homeostasis by inflammatory immune responses. Novel strategies for ameliorating IBD have been proposed. The study showed that different strains of Lactococcus lactis improved clinical scores and parameters of colitis, confirming their anti-inflammatory properties in this model.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Food Science & Technology
Patryk Pokorski, Monika Trzaskowska
Summary: This research focused on characterizing the fermentation system of L. lactis ATCC 11454 and barley products, especially the inactivation of selected Bacillus strains during fermentation and storage. It was found that co-fermentation with Bacillus strains significantly reduced their numbers in barley products, improving the safety and nutritional value of food.
Article
Biotechnology & Applied Microbiology
Marta Kis, Nevijo Zdolec, Snjezana Kazazic, Marko Vincekovic, Slaven Juric, Vesna Dobranic, Fabijan Ostaric, Ivan Maric, Natasa Mikulec
Summary: This study developed a microencapsulated form of selected autochthonous lactic-acid bacteria (LAB) and natural rennet for the production of traditional hard sheep cheese, Paski sir. The microsphere formulations containing LAB and rennet showed no negative interactions and allowed for the production of cheese with the same characteristics as the commercial product. This pilot study demonstrated the potential of combining traditional and sustainable innovative technologies in cheese production to maintain authenticity.
FERMENTATION-BASEL
(2023)
Article
Oncology
Margaret Jia, Naseer Sangwan, Alice Tzeng, Charis Eng
Summary: This observational report evaluated potential contributors to autoimmunity and other immune dysregulation phenotypes in individuals with germline mutations in the tumor suppressor gene PTEN. The study found that PTEN-associated immune phenotypes might be influenced by gut microbiome and class II HLA molecules, indicating a potential crosstalk between genetics and gut microbiome in immune regulation.
JCO PRECISION ONCOLOGY
(2021)
Article
Food Science & Technology
Chen Chen, Jiajie Yuan, Haiyan Yu, Xinman Lou, Bei Wang, Zhiyuan Xu, Huaixiang Tian
Summary: This study discovered three variants of branched-chain alpha-keto acid decarboxylase (KADC) in Lactococcus lactis strains with different abilities to produce 3-methylbutanal. The KADC-long variant was found to have activity in Escherichia coli cloning experiments. KADC-long showed efficient production of 2-methylbutanal and 3-methylbutanal under optimal conditions.
Article
Infectious Diseases
Myrte Tielemans, Mireille van Westreenen, Corne Klaassen, Hannelore M. Gotz
Summary: This study investigated the effect of confirmatory testing on Neisseria gonorrhoeae (Ng) positivity in a population visiting a sexual health clinic in Rotterdam. Confirming urogenital and anorectal samples reduced Ng positivity rates, especially for women. The lack of a gold standard for Ng infection confirmation presents a dilemma in public health settings: choosing between missed diagnoses or overtreatment.
SEXUALLY TRANSMITTED INFECTIONS
(2022)
Article
Microbiology
Raka Choudhury, Michiel Kleerebezem
Summary: This study investigated the impact of diet on the mucosa-adhered microbiota in pre-weaning piglets by analyzing rectal swabs and colon samples. The results showed variable enrichment of adhered populations in rectal swabs and suggested that early life feeding may suppress post-weaning diarrhea. Furthermore, the paired swab-colon microbiota information can be used to predict the adhered microbiota in rectal swab samples.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Environmental Sciences
Onur Ercan, Heidy M. W. Besten, Eddy J. Smid, Michiel Kleerebezem
Summary: This study reveals the strategies of lactococcus to adapt to long-term survival in oligotrophic environments by activating stress-associated functions and increasing stress tolerance. It also illustrates the trade-off between growth and survival in the gene regulation network of lactococcus.
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2022)
Article
Public, Environmental & Occupational Health
Anne F. Voor in 't Holt, Adrienne S. van der Schoor, Kees Mourik, Nikolaos Strepis, Corne H. W. Klaassen, Margreet C. Vos, Juliette A. Severin
Summary: This study investigated the travel history and behavior of patients and their correlation to highly resistant microorganism (HRMO) carriage upon hospital admission. It found that half of the admitted patients had traveled abroad in the last year, with only a small percentage traveling outside Europe. The majority of patients were aware of the risk of HRMO carriage during international travel and supported screening strategies to minimize the risk of introducing HRMO.
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2022)
Article
Microbiology
Avis Dwi Wahyu Nugroho, Berdien van Olst, Stephanie Agnes Bachtiar, Sjef Boeren, Michiel Kleerebezem, Herwig Bachmann
Summary: This article investigates the influence of manganese on lactococcal metabolic persistence. The experiments show that in the absence of manganese, acidification of non-growing cells can last for weeks, while in the presence of manganese, it stops within 1 day. The study also finds that the effects of manganese are NADH dependent.
MICROBIOLOGY SPECTRUM
(2022)
Article
Multidisciplinary Sciences
S. Duboux, J. A. Muller, F. De Franceschi, A. Mercenier, M. Kleerebezem
Summary: Bifidobacteria, the early colonizers of the human gastrointestinal system, are crucial for gut development and disease prevention. This study developed a fluorescent protein-based reporter system to investigate carbohydrate metabolism in B. longum NCC 2705. The results revealed the induction of specific promoters by different carbohydrates and highlighted the complexity of carbohydrate consumption patterns in this strain.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Dimard E. Foudraine, Lennard J. M. Dekker, Nikolaos Strepis, Stan J. Nispeling, Merel N. Raaphorst, Wendy Kloezen, Piet Colle, Annelies Verbon, Corne H. W. Klaassen, Theo M. Luider, Wil H. F. Goessens
Summary: A multiplex-targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay was developed and validated for the rapid and accurate detection of resistance mechanisms in Escherichia coli or Klebsiella pneumoniae complex from positive blood cultures. The assay showed high agreement with whole-genome sequencing results and correctly predicted the majority of susceptible and non-susceptible phenotypes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Manisha Goyal, Andreu Coello Pelegrin, Magali Jaillard, Yulia Rosa Saharman, Corne H. W. Klaassen, Henri A. Verbrugh, Juliette A. Severin, Alex van Belkum
Summary: This study demonstrates the importance of detailed whole genome sequencing analysis for highly refined epidemiological analysis of carbapenem non-susceptible Pseudomonas aeruginosa. The analysis revealed variations in transmission and acquisition of CNPA strains among patients, as well as the correlation between genomic sequence changes and the distribution of antibiotic resistance and virulence genes.
FRONTIERS IN MICROBIOLOGY
(2022)
Correction
Public, Environmental & Occupational Health
Adrienne S. van der Schoor, Juliette A. Severin, Anna S. van der Weg, Nikolaos Strepis, Corne H. W. Klaassen, Johannes P. C. van den Akker, Marco J. Bruno, Johanna M. Hendriks, Margreet C. Vos, Anne Voor F. In 't Holt
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2022)
Article
Immunology
Sammy Huygens, Albert Dunbar, Jochem B. Buil, Corne H. W. Klaassen, Paul E. Verweij, Karin van Dijk, Nick de Jonge, Jeroen J. W. M. Janssen, Walter J. F. M. van der Velden, Bart J. Biemond, Aldert Bart, Anke H. W. Bruns, Pieter-Jan A. Haas, Astrid M. P. Demandt, Guy Oudhuis, Peter von dem Borne, Martha T. van der Beek, Saskia K. Klein, Peggy Godschalk, Lambert F. R. Span, Douwe F. Postma, Greetje A. Kampinga, Johan Maertens, Katrien Lagrou, Toine Mercier, Ine Moors, Jerina Boelens, Dominik Selleslag, Marijke Reynders, Willemien Zandijk, Jeanette K. Doorduijn, Jan J. Cornelissen, Alexander F. A. D. Schauwvlieghe, Bart J. A. Rijnders
Summary: This prospective multicenter study showed that real-time resistance testing may limit the impact of azole resistance on mortality. An isolated positive polymerase chain reaction assay was not associated with mortality. Its place in the current EORTC/MSGERC definitions should be reconsidered.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Linda J. J. Wammes, Anne F. Voor in F. 't Holt, Corne H. W. Klaassen, Margreet C. C. Vos, Nelianne J. J. Verkaik, Juliette A. Severin
Summary: This study aimed to improve the sensitivity of detecting vancomycin-resistant Enterococcus faecium (VRE) transmission and determine the time from presumed exposure to detectable colonization. Retrospective analysis of 9 VRE outbreaks showed that using the first three out of five rectal swabs identified 89% of all secondary cases, compared to only 64% with the first swab. In addition, taking one or more rectal swabs around day 9 after presumed exposure was found to be the most effective screening approach.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Public, Environmental & Occupational Health
Adrienne S. van der Schoor, Juliette A. Severin, Corne H. W. Klaassen, Diederik Gommers, Marco J. Bruno, Johanna M. Hendriks, Anne F. Voor in 't Holt, Margreet C. Vos
Summary: This study aimed to compare the environmental contamination with highly resistant microorganisms (HRMO) between an old hospital building and a new hospital building, and to investigate the environmental contamination in the new building after relocation. The results showed a significant decrease in HRMO contamination in the new building, which is important for hospital design and construction considerations.
INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH
(2023)
Article
Multidisciplinary Sciences
Jannette Pirzadian, Anne F. Voor In 't Holt, Mehjabeen Hossain, Corne H. W. Klaassen, Inge de Goeij, Heidy H. H. T. Koene, Lonneke G. M. Bode, Margreet C. Vos, Juliette A. Severin
Summary: In healthcare environments, sinks are recognized as reservoirs for multidrug-resistant bacteria. This study implemented an intervention to install sink drain plugs as barriers against transmission of VIM-PA from drain reservoirs. Results showed a significant reduction in VIM-PA positivity after the intervention, but recolonization was observed over time.
Article
Multidisciplinary Sciences
Adrienne S. van der Schoor, Juliette A. Severin, Corne H. W. Klaassen, Johannes P. C. van den Akker, Marco J. Bruno, Johanna M. Hendriks, Margreet C. Vos, Anne F. Voor F. In 't Holt
Summary: Timely identification of patients carrying multidrug-resistant microorganisms is crucial to prevent nosocomial spread. This study compared the yield of a universal screening strategy upon admission to the current risk assessment combined with risk-based screening, showing that universal screening identified significantly more carriers in a low endemic setting.
Article
Microbiology
Sieze Douwenga, Berdien van Olst, Sjef Boeren, Yanzhang Luo, Xin Lai, Bas Teusink, Jacques Vervoort, Michiel Kleerebezem, Herwig Bachmann
Summary: Bacteria like Lactococcus cremoris have a hierarchical sugar catabolism system, where they consistently prioritize the catabolization of higher-quality sugars for optimal growth rate, but also have some proteins always present for consuming certain lower-quality sugars.
MICROBIOLOGY SPECTRUM
(2023)
