Article
Biotechnology & Applied Microbiology
Leandro Lopes da Silva, Tulio Morgan, Ediones Amaro Garcia, Rafael Oliveira Rosa, Tiago Antonio de Oliveira Mendes, Marisa Vieira de Queiroz
Summary: This study aimed to identify and analyze genes encoding pectinases in the genome of the fungus Colletotrichum lindemuthianum, evaluate their expression, and compare them with pectinases from other fungi. The results showed that C. lindemuthianum has a rich variety of pectinase genes, which may play a crucial role in its pathogenicity. The findings contribute to a better understanding of the importance of pectinases in common bean infection and have potential applications in biotechnology.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Review
Food Science & Technology
Vashishtha B. Patel, Somak Chatterjee, Abhishek S. Dhoble
Summary: Pectinase enzymes are biological compounds that degrade pectic compounds in fruit juices, making them excellent biocatalysts for juice clarification. However, the poor stability and nonreusability of pectinases limit their effectiveness. Immobilization techniques, particularly using membranes, have been shown to enhance the performance of enzymes. This review highlights the importance of physicochemical properties and kinematic properties of pectinases in relation to juice processing parameters and discusses the potential of membrane immobilization as a support for pectinase.
JOURNAL OF FOOD SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
C. Liu, X. Qin, B. Liu, X. Xu, A. Deng, Y. Zhang, Z. Zhang, W. Zhang
Summary: A pectin lyase gene pnlzj5b from Aspergillus niger ZJ5 was successfully identified and overexpressed in Pichia pastoris. The recombinant protein PNLZJ5B showed high activity towards citrus pectin and its expression efficiency was improved through codon optimization. PNLZJ5B exhibited optimal activity and stability at specific temperature and pH, and it also showed positive response to the degree of pectin methylation and had a higher juice yield compared to commercial pectin lyase. These favorable enzymatic properties make PNLZJ5B potentially useful in juice processing and other food-related applications.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Food Science & Technology
Nicole Jasmin Nemetz, Anne Ruth Winter, Jan-Peter Hensen, Andreas Schieber, Fabian Weber
Summary: Current fruit juice production aims for sustainable processes with high extraction yields and reduced by-products. Controlled degradation of cell wall polysaccharides, specifically pectin, can help minimize the generation of side streams. This study investigated the effects of ultrasound-assisted enzymatic maceration on chokeberry juice production, including pectin degradation, anthocyanin content, thermal and storage stability, and juice yield.
CURRENT RESEARCH IN FOOD SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Hiromitsu Suzuki, Toshiki Morishima, Atsuya Handa, Hironaka Tsukagoshi, Masashi Kato, Motoyuki Shimizu
Summary: Pectinolytic enzymes, including pectate lyases, have diverse industrial applications. In this study, the biochemical characterization of the pectate lyase AnPL9 from the filamentous fungus Aspergillus nidulans was performed. AnPL9 exhibited high activity on various pectin substrates and showed stability within a neutral-to-alkaline pH range. These findings suggest that AnPL9 has potential biotechnological applications in the food, paper, and textile industries.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Medicinal
Xiaohan Qiu, Lizhi Gong, Xiujuan Xin, Faliang An
Summary: Emodin, a widely distributed anthraquinone derivative, can be efficiently produced by marine-derived fungus Aspergillus favipes HN4-13. Optimization of fermentation conditions led to successful production of emodin with high yield.
Review
Food Science & Technology
Feng Wang, Hui Xu, Miaomiao Wang, Xiaolei Yu, Yi Cui, Ling Xu, Anzhou Ma, Zhongyang Ding, Shuhao Huo, Bin Zou, Jingya Qian
Summary: This article provides a systematic review of the methods of enzyme immobilization and the use of immobilized supports in juice clarification. It gives detailed explanations of the mechanisms and effects of immobilized pectinase, immobilized laccase, and immobilized xylanase in fruit juice clarification. Suggestions and prospects for future studies in this area are also provided.
Review
Food Science & Technology
Vadym Chibrikov, Polina Vakuliuk, Henryk Sobczuk
Summary: Sweet sorghum is a promising biomaterial due to its nutritional and energy value, easy cultivation, and promising economic parameters. This review provides comprehensive information on the physicochemical properties of sweet sorghum juice and sirup, as well as the technological details of their processing. It focuses on the relationship between juice treatment methods and composition, serving as a versatile source of information for the food science community, farmers, and entrepreneurs.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Microbiology
Laila Naher, Siti Noor Fatin, Md Abdul Halim Sheikh, Lateef Adebola Azeez, Shaiquzzaman Siddiquee, Norhafizah Md Zain, Sarker Mohammad Rezaul Karim
Summary: Fungi play important roles in various sectors, with the isolation of potential fungal species being crucial for their utilization. This study isolated filamentous fungi from soil and weed leaves and evaluated their cellulase enzyme production capabilities. Results showed that Trichoderma reesei produced a significantly higher amount of cellulase compared to Aspergillus awamori.
Article
Microbiology
Zafar Ali Shah, Khalid Khan, Haroon Ur Rashid, Tanzeel Shah, Mariusz Jaremko, Zafar Iqbal
Summary: This study explored the metabolic profile of Aspergillus ficuum and identified bioactive compounds. Pharmacological screening showed that A. ficuum extract exhibited antibacterial and anti-inflammatory effects with no acute toxicity, which was supported by docking results.
Review
Biochemistry & Molecular Biology
Kanchan Yadav, Shruti Dwivedi, Supriya Gupta, Aiman Tanveer, Sangeeta Yadav, Pramod K. Yadav, Gautam Anand, Dinesh Yadav
Summary: Pectin, a fundamental component of plant cell walls, is degraded by pectin lyases which have unique characteristics and play important roles in various industries such as juice processing. This review provides updates on the sources, production strategies, biochemical attributes, and molecular insights of pectin lyases.
PROCESS BIOCHEMISTRY
(2023)
Article
Engineering, Chemical
Yanmei Meng, Shuangshuang Yu, Zhenyong Qiu, Jinlai Zhang, Jianfan Wu, Tao Yao, Johnny Qin
Summary: In the clarification process of sugarcane juice, a data-driven model based on the deep kernel extreme learning machine was constructed to predict key production indicators, successfully predicting clear juice color value and gravity purity by combining kernel method and multi-layer extreme learning machine. An optimization strategy for operational variables was proposed, ensuring optimal set of key operational variables under typical working conditions through the niche multi-objective particle swarm optimization algorithm.
JOURNAL OF FOOD ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Yuying Ma, Chukun Wang, Zhen Gao, Yuxin Yao, Hui Kang, Yuanpeng Du
Summary: The ripening and softening process in grape is associated with the depolymerization of pectin components, which is facilitated by pectin lyases (PLs). This study identified 16 VvPL genes in the grape genome, and found that VvPL5, VvPL9, and VvPL15 are highly expressed during grape ripening, indicating their involvement in grape softening. Overexpression of VvPL15 in Arabidopsis affected pectin content and plant growth, while antisense expression of VvPL15 confirmed the relationship between VvPL15 and pectin content. Additionally, transgenic tomato plants with VvPL15 showed accelerated fruit ripening and softening. Thus, VvPL15 plays a crucial role in grape berry softening by depolymerizing pectin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agronomy
Elena Cristina Scutarasu, Camelia Elena Luchian, Ionel Bogdan Cioroiu, Lucia Carmen Trinca, Valeriu V. Cotea
Summary: This research focuses on the effect of enzymes on amino acid concentration during the fermentation of Feteasca regala and Sauvignon blanc wines. The results show that pectinases have a significant impact on the sensory characteristics of the wines, with Feteasca regala wines showing a greater response to pectinase treatment and Sauvignon blanc wines showing a higher response to beta-glycosides.
Article
Chemistry, Applied
Jessika N. Humerez-Flores, Clare Kyomugasho, Ana A. Gutierrez-Ortiz, Margot De Bie, Agnese Panozzo, Ann M. Van Loey, Paula Moldenaers, Marc E. Hendrickx
Summary: Tailored citrus pectin-derived compounds were produced through controlled enzymatic and chemical modifications, yielding compounds with different degrees of methylesterification and molecular weights. These compounds will be further studied for exploring their functionality.
Article
Chemistry, Multidisciplinary
Pramod K. Yadav, Michael Martinov, Victor Vitvitsky, Javier Seravalli, Rudolf Wedmann, Milos R. Filipovic, Ruma Banerjee
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2016)
Article
Biochemistry & Molecular Biology
Sebastian Carballal, Ernesto Cuevasanta, Pramod K. Yadav, Carmen Gherasim, David P. Ballou, Beatriz Alvarez, Ruma Banerjee
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Biochemistry & Molecular Biology
Victor Vitvitsky, Pramod K. Yadav, Sojin An, Javier Seravalli, Uhn-Soo Cho, Ruma Banerjee
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Victor Vitvitsky, Jan Lj Miljkovic, Trever Bostelaar, Bikash Adhikari, Pramod K. Yadav, Andrea K. Steiger, Roberta Torregrossa, Michael D. Pluth, Matthew Whiteman, Ruma Banerjee, Milos R. Filipovic
ACS CHEMICAL BIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Pramod K. Yadav, Victor Vitvitsky, Hanseong Kim, Andrew White, Uhn-Soo Cho, Ruma Banerjee
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Pramod Kumar Yadav, Victor Vitvitsky, Sebastian Carballal, Javier Seravalli, Ruma Banerjee
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Lauren E. Williamson, Theron Gilliland, Pramod K. Yadav, Elad Binshtein, Robin Bombardi, Nurgun Kose, Rachel S. Nargi, Rachel E. Sutton, Clarissa L. Durie, Erica Armstrong, Robert H. Carnahan, Lauren M. Walker, Arthur S. Kim, Julie M. Fox, Michael S. Diamond, Melanie D. Ohi, William B. Klimstra, James E. Crowe
Article
Multidisciplinary Sciences
Sarah M. Fantin, Kristine F. Parson, Pramod Yadav, Brock Juliano, Geoffrey C. Li, Charles R. Sanders, Melanie D. Ohi, Brandon T. Ruotolo
Summary: Peripheral myelin protein (PMP22) misfolding is identified as a key factor in various peripheral neuropathies. Mutant forms of PMP22 exhibit differences in stability and propensity to form homodimeric complexes compared to wild-type protein. The formation of PMP22 dimers from destabilized monomers is proposed as a key element in PMP22 mistrafficking, based on combined findings from native ion mobility-mass spectrometry and cellular data.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Agronomy
Shubhra Gupta, Kapil Gupta, Chitra Nehra, R. K. Gaur, Dinesh Yadav
Summary: Sugarcane is a major cash crop globally, but it faces challenges from various stresses. Conventional breeding approaches have limitations, but recent advancements in omics-driven research provide new opportunities for developing salt-tolerant sugarcane varieties. The use of genomics, proteomics, and genome-editing technologies has greatly enhanced the pace of sugarcane improvement.
Article
Biotechnology & Applied Microbiology
Kanchan Yadav, Shruti Dwivedi, Supriya Gupta, Amit K. Dubey, Vinay K. Singh, Aiman Tanveer, Sangeeta Yadav, Dinesh Yadav
Summary: In this study, 52 pectin lyase genes were predicted and characterized in six species of Fusarium. The protein sequences showed variability in terms of amino acid residues, molecular weight, and pI. Multiple sequence alignment and phylogenetic analysis revealed conserved residues and distinct groups. Three-dimensional structure prediction and analysis showed diverse structural motifs. The study provides important insights into the diversity and potential applications of pectin lyase enzymes.
Article
Biochemistry & Molecular Biology
Surabhi Suchanti, Sonali Awasthi, Gyanendra Singh, Pramod K. Yadav, Abhijeet Singh, Rajeev Mishra
Summary: Lung cancer and COVID-19 present a severe combination due to the lungs being the primary target organ for SARS-CoV-2 infection. The study identified a potential correlation between immune-modulators and receptor proteins that requires further laboratory research for clinical validation.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2022)
Review
Plant Sciences
Varsha Rani, D. C. Joshi, Priyanka Joshi, Rajesh Singh, Dinesh Yadav
Summary: The main purpose of this review is to shed light on the role of millet models in imparting climate resilience and nutritional security and to give a concrete perspective on how NF-Y transcription factors can be harnessed for making cereals more stress tolerant. Agriculture faces significant challenges from climate change, bargaining, population, elevated food prices, and compromises with nutritional value.
Review
Plant Sciences
Rupali Gupta, Gautam Anand, Rajeeva Gaur, Dinesh Yadav
Summary: Plant-microbiome interactions play a significant role in nutrient acquisition and defense mechanisms of the host plant. Understanding and utilizing plant microbiomes can have implications for future agricultural applications.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2021)
Article
Biochemistry & Molecular Biology
Aaron P. Landry, Sojin Moon, Hanseong Kim, Pramod K. Yadav, Arkajit Guha, Uhn-Soo Cho, Ruma Banerjee
CELL CHEMICAL BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Markus Ruetz, Gregory C. Campanello, Liam McDevitt, Adam L. Yokom, Pramod K. Yadav, David Watkins, David S. Rosenblatt, Melanie D. Ohi, Daniel R. Southworth, Ruma Banerjee
CELL CHEMICAL BIOLOGY
(2019)