Article
Biochemistry & Molecular Biology
Rakesh Mazumdar, Baishakhi Mondal, Shankhadeep Saha, Bapan Samanta, Biplab Mondal
Summary: A nitrosyl complex of cobalt(II) porphyrinate has been synthesized and characterized. It reacts with superoxide ion to form nitrite complex, suggesting the involvement of a cobalt(II)-peroxynitrite intermediate.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Review
Physiology
Lucia Piacenza, Ari Zeida, Madia Trujillo, Rafael Radi
Summary: The free radical nitric oxide (NO) plays a key role in physiological processes, but its bioavailability is compromised in certain disease conditions due to increased production of superoxide radical. This leads to the inactivation of nitric oxide and the formation of peroxynitrite, which is implicated in various human diseases and the aging process. Understanding the complex biochemistry of peroxynitrite is crucial for developing therapeutic interventions.
PHYSIOLOGICAL REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Elizabeth A. Sweeny, Andrew P. Hunt, Allison E. Batka, Simon Schlanger, Nicolai Lehnert, Dennis J. Stuehr
Summary: Nitric oxide (NO) serves as a ubiquitous cell signaling molecule, mediating various processes by activating or inactivating protein targets. Research shows that NO can increase NOX5 activity through heme-dependent effects, shedding light on NOX5 regulation and the impact of NO on hemeprotein activity.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Chemistry, Multidisciplinary
Joo-Eun Kim, Hyun-Wook Lee, Won-Jin Kwak
Summary: In this study, 4-carboxy-TEMPO was selected as a representative SODm, and its ability to effectively promote the disproportionation reaction and accelerate the generation of singlet oxygen was chemically verified. It was found that the release of singlet oxygen evolved by the SODm was decreased when using a quencher. This study provides important reference for the development of additives for practical application of LOBs.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Mary Grace I. Galinato, Aaron M. Trail, Olivia R. Steinbeck, Zhuoyan Si, Anthony M. Rodland, Jaclyn Gowen
Summary: The esterification of heme propionates results in a minor structural change that significantly increases the NiR rate in DME-Mb. The lower pKa observed in DME-Mb enhances the tendency of His64 towards protonation, leading to an increase in the NiR rate. The nitrite binding constant for DME-MbIII is also greater than wt MbIII, indicating a correlation between nitrite reductase activity and electrostatic behavior.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Jinghua Chen, Peilu Xie, Yujia Huang, Haichun Gao
Summary: Nitrite and nitric oxide are critical nitrogen oxides in the nitrogen biogeochemical cycle, capable of interacting with heme-copper oxidases for enzymatic interconversion and inhibition. While they show similar biochemical properties, they are treated as distinct molecules by bacterial cells and have different effects on enzymes and sensing systems.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Plant Sciences
John T. Hancock
Summary: Inert gases like xenon and molecular hydrogen have significant biological effects, possibly through their interaction with protein structures. Hydrogen may also interact with redox active compounds, while the possibility of these compounds interacting with xenon pockets and cavities in proteins is being explored.
Article
Medicine, Research & Experimental
Xiang Gao, Kou Liu, Chengyang Hu, Keyang Chen, Zhengxuan Jiang
Summary: This study demonstrates that captopril alleviates oxidative damage in diabetic retinopathy by reducing reactive oxygen species (ROS) and nitric oxide (NO) levels, thereby reducing peroxynitrite formation. These findings provide new directions for research in diabetic retinopathy.
Article
Chemistry, Physical
Yunxiang Meng, Junye Ma, Yiqi Yan, Zongsu Wei, Richard Spinney, Dionysios D. Dionysiou, Ruiyang Xiao
Summary: Phosphite (PHI) is an important component of aquatic systems and understanding its transformation to phosphate is crucial. This study investigates the role of superoxide radicals (O center dot-2) in PHI transformation using direct and indirect methods. The results provide valuable insights into the degradation kinetics and mechanisms of PHI and O center dot-2.
SURFACES AND INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Dmytro V. Kravchuk, Nicholas N. Dahlen, Samantha J. Kruse, Christos D. Malliakas, Paul M. Shand, Tori Z. Forbes
Summary: The high radiation field associated with spent nuclear fuel generates reactive radical species that affect corrosion and formation of secondary alteration phases. A study revealed that the interaction between dioxygen radicals and UO22+ ions has a significant impact on the alteration phases of spent nuclear fuel, with the formation of a U-VI superoxide compound. Additionally, the chemistry of uranyl superoxide plays a crucial role in the prevalence of carbonate in the secondary phases of spent nuclear fuel.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Pritam Mondal, Garrett B. Tolbert, Gayan B. Wijeratne
Summary: This study investigates the low temperature reactivities of high-valent heme intermediates with nitric oxide (NO), revealing two different reaction pathways. The findings highlight the potential of high-valent heme species in catalyzing efficient NOx interconversions, shedding light on unexplored territory central to human physiology and therapeutics.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yunlong Zheng, Wenwen Deng, Di Liu, Youheng Li, Kang Peng, George H. Lorimer, Jun Wang
Summary: In this review, the authors summarize the redox and spectroscopic properties of nitrite reductase-like hemoproteins in mammals. These proteins exhibit nitrite reductase-like activity in vitro and are closely related to their redox and spectroscopic properties and protein structure, although the physiological roles of Ngb, Cygb, and IDO1 remain unclear.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Materials Science, Biomaterials
Ping Huang, Zihong Li, Li Nong, Jie Cheng, Weiying Lin
Summary: Aging is a natural process characterized by the decline of physiological functions in the body's organs and tissues. Oxidative stress, caused by active nitrogen substances like ONOO-, is considered to be the main cause of aging. Detecting changes in ONOO- in aging cells is important for studying senescence. In this study, we developed a fluorescent probe FLASN based on flavonol that can detect and treat ONOO- in cells. Our results showed that FLASN can effectively detect ONOO- in cells and reduce its production stimulated by metformin. This study provides a new perspective on the role of ONOO- in senescence and the potential therapeutic effect of flavonol.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Engineering, Chemical
Jinying Du, Chuang Wang, Zhiwei Zhao, Fuyi Cui, Qin Ou, Jie Liu
Summary: Vacuum UV radiation can generate hydroxyl radicals and H2O2 through the homolysis and ionization of water. Sufficient dissolved oxygen promotes the production and formation rate of H2O2, while UV254 radiation induces the decomposing of H2O2. The study also highlights the unfavorable effects of alkaline conditions and certain anions on H2O2 production.
CHEMICAL ENGINEERING SCIENCE
(2021)
Article
Environmental Sciences
Adeniyi Olufemi Adesina, Hiroshi Sakugawa
Summary: The study investigated the consumption mechanism of photochemically generated peroxynitrite in seawater, finding that the existence of peroxynitrite strongly depends on and is limited by photoformed NO center dot. Seawater alkalinity favors the consumption of photoformed NO center dot via the peroxynitrite sink. The experimental data suggest that the consumption of photochemically generated NO center dot via the peroxynitrite sink is not significant in surface seawater.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biochemistry & Molecular Biology
Romila Mascarenhas, Zhu Li, Carmen Gherasim, Markus Ruetz, Ruma Banerjee
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Pathology
David M. Manthei, Jason F. Whalen, Lee F. Schroeder, Anthony M. Sinay, Shih-Hon Li, Riccardo Valdez, Donald A. Giacherio, Carmen Gherasim
Summary: With the passage of time from symptom onset, samples from SARS-CoV-2 RT-PCR-positive patients showed increasing positivity. The sensitivity of EUROIMMUN, Roche, and Siemens assays reached 93.1% to 96.6%, 98.3%, and 96.6%, respectively, for patients with the latest sample collected 14 or more days after symptom onset, outperforming the DiaSorin assay at 87.7%. The specificity of Roche and Siemens assays was 100%, higher than DiaSorin and EUROIMMUN assays.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Dayana Benchoam, Ernesto Cuevasanta, Laia Julio Plana, Luciana Capece, Ruma Banerjee, Beatriz Alvarez
Summary: This study investigates the kinetics of reactions between CBS and mercury compounds, revealing that Fe(II)-CBS reacts faster than Fe(III)-CBS, and the rate constants of the reactions increase hyperbolically with the concentration of mercury compounds.
Article
Biochemistry & Molecular Biology
Lucia Turell, Martina Steglich, Maria J. Torres, Matias Deambrosi, Laura Antmann, Cristina M. Furdui, Francisco J. Schopfer, Beatriz Alvarez
Summary: This study confirmed the formation process of HSA-SOH and its further oxidation to sulfinic acid and sulfonic acid in the presence of hydrogen peroxide. The decay pathways of HSA-SOH were investigated, revealing its reaction with thiols to form mixed disulfides.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Multidisciplinary Sciences
Charles F. Schuler, Carmen Gherasim, Kelly O'Shea, David M. Manthei, Jesse Chen, Don Giacherio, Jonathan P. Troost, James L. Baldwin, James R. Baker
Summary: The study conducted COVID-19 antibody testing in high-risk populations at the point of care, finding that finger-stick blood antibody testing was significantly effective in identifying antibodies from prior infections. Using serum did not improve the sensitivity or specificity of the testing, and almost all individuals with COVID-19 infection produced detectable antibodies to the virus.
Article
Multidisciplinary Sciences
Sarah M. Fantin, Kristine F. Parson, Pramod Yadav, Brock Juliano, Geoffrey C. Li, Charles R. Sanders, Melanie D. Ohi, Brandon T. Ruotolo
Summary: Peripheral myelin protein (PMP22) misfolding is identified as a key factor in various peripheral neuropathies. Mutant forms of PMP22 exhibit differences in stability and propensity to form homodimeric complexes compared to wild-type protein. The formation of PMP22 dimers from destabilized monomers is proposed as a key element in PMP22 mistrafficking, based on combined findings from native ion mobility-mass spectrometry and cellular data.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Masaki Imai, Peter J. Halfmann, Seiya Yamayoshi, Kiyoko Iwatsuki-Horimoto, Shiho Chiba, Tokiko Watanabe, Noriko Nakajima, Mutsumi Ito, Makoto Kuroda, Maki Kiso, Tadashi Maemura, Kenta Takahashi, Samantha Loeber, Masato Hatta, Michiko Koga, Hiroyuki Nagai, Shinya Yamamoto, Makoto Saito, Eisuke Adachi, Osamu Akasaka, Morio Nakamura, Ichiro Nakachi, Takayuki Ogura, Rie Baba, Kensuke Fujita, Junichi Ochi, Keiko Mitamura, Hideaki Kato, Hideaki Nakajima, Kazuma Yagi, Shin-Ichiro Hattori, Kenji Maeda, Tetsuya Suzuki, Yusuke Miyazato, Riccardo Valdez, Carmen Gherasim, Yuri Furusawa, Moe Okuda, Michiko Ujie, Tiago J. S. Lopes, Atsuhiro Yasuhara, Hiroshi Ueki, Yuko Sakai-Tagawa, Amie J. Eisfeld, John J. Baczenas, David A. Baker, Shelby L. O'Connor, David H. O'Connor, Shuetsu Fukushi, Tsuguto Fujimoto, Yudai Kuroda, Aubree Gordon, Ken Maeda, Norio Ohmagari, Norio Sugaya, Hiroshi Yotsuyanagi, Hiroaki Mitsuya, Tadaki Suzuki, Yoshihiro Kawaoka
Summary: The P.1 variant of SARS-CoV-2 showed similar replicative abilities and pathogenicity in Syrian hamsters compared to early and contemporary strains. Sera/plasma from convalescent patients and BNT162b2 vaccinees had comparable neutralization titers against the P.1 variant, S-614D, and S-614G strains. Serum from a P.1-infected patient recognized the S-614D and S-614G strains less effectively than the P.1 variant.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Charles F. Schuler, Carmen Gherasim, Kelly O'Shea, David M. Manthei, Jesse Chen, Cristyn Zettel, Jonathan P. Troost, Andrew A. Kennedy, Andrew W. Tai, Donald A. Giacherio, Riccardo Valdez, James L. Baldwin, James R. Baker
Summary: This study demonstrates that individuals with mild COVID-19 who produce antibodies are protected from reinfection for up to 6 months. The antibodies produced in this situation remain stable for up to 6 months and correlate well with neutralization activity.
MICROBIOLOGY SPECTRUM
(2021)
Article
Pediatrics
Kriselle Lao, Elizabeth Sykes, Xander M. R. van Wijk, Jieli Li, JoAnna Williams, Carmen Gherasim, Qian Sun
Summary: Serum creatinine concentration is a crucial factor in estimating glomerular filtration rate (eGFR) in children. However, many commonly used creatinine assays show inaccuracy, especially at low concentrations, which can result in misclassification of chronic kidney disease and inappropriate interventions in pediatric populations.
PEDIATRIC NEPHROLOGY
(2022)
Article
Multidisciplinary Sciences
Ryuta Uraki, Maki Kiso, Shun Iida, Masaki Imai, Emi Takashita, Makoto Kuroda, Peter J. Halfmann, Samantha Loeber, Tadashi Maemura, Seiya Yamayoshi, Seiichiro Fujisaki, Zhongde Wang, Mutsumi Ito, Michiko Ujie, Kiyoko Iwatsuki-Horimoto, Yuri Furusawa, Ryan Wright, Zhenlu Chong, Seiya Ozono, Atsuhiro Yasuhara, Hiroshi Ueki, Yuko Sakai-Tagawa, Rong Li, Yanan Liu, Deanna Larson, Michiko Koga, Takeya Tsutsumi, Eisuke Adachi, Makoto Saito, Shinya Yamamoto, Masao Hagihara, Keiko Mitamura, Tetsuro Sato, Masayuki Hojo, Shin-ichiro Hattori, Kenji Maeda, Riccardo Valdez, Moe Okuda, Jurika Murakami, Calvin Duong, Sucheta Godbole, Daniel C. Douek, Ken Maeda, Shinji Watanabe, Aubree Gordon, Norio Ohmagari, Hiroshi Yotsuyanagi, Michael S. Diamond, Hideki Hasegawa, Hiroaki Mitsuya, Tadaki Suzuki, Yoshihiro Kawaoka
Summary: The replicative ability and pathogenicity of Omicron BA.2 variant is similar to that of BA.1 in rodents, but it shows less pathogenicity compared to early SARS-CoV-2 strains. There is a marked reduction in the neutralizing activity of plasma from individuals who had recovered from COVID-19 and vaccine recipients against BA.2 variant.
Letter
Medicine, General & Internal
Qian Wang, Anthony Bowen, Riccardo Valdez, Carmen Gherasim, Aubree Gordon, Lihong Liu, David D. D. Ho
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Sofia Lima, Juan Blanco, Federico Olivieri, Juan A. Imelio, Marcos Nieves, Federico Carrion, Beatriz Alvarez, Alejandro Buschiazzo, Marcelo A. Marti, Felipe Trajtenberg
Summary: Phosphorylation carries chemical information in biological systems, and in two-component systems, uni- or bidirectional phosphoryl transfer reactions connect the sensor histidine kinase and response regulator. Directionality in phosphoryl transfer enables the construction of complex regulatory networks that optimize signal propagation and ensure the forward flow of information.
Article
Biochemistry & Molecular Biology
Surabhi Suchanti, Sonali Awasthi, Gyanendra Singh, Pramod K. Yadav, Abhijeet Singh, Rajeev Mishra
Summary: Lung cancer and COVID-19 present a severe combination due to the lungs being the primary target organ for SARS-CoV-2 infection. The study identified a potential correlation between immune-modulators and receptor proteins that requires further laboratory research for clinical validation.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2022)
Article
Immunology
Kelly M. O'Shea, Charles F. Schuler, Jesse Chen, Jonathan P. Troost, Pamela T. Wong, Kelsea Chen, Daniel R. O'Shea, Westley Peng, Carmen Gherasim, David M. Manthei, Riccardo Valdez, James L. Baldwin, Jr James R. Baker
Summary: This study examines the degree of immune protection against Delta and Omicron variants provided by infection and vaccination with wild-type SARS-CoV-2. The results show that individuals with both infection and vaccination have the highest neutralizing antibody levels against all variants, and neutralization of Omicron is better correlated with evidence of prior infection. This suggests that better protection against Omicron is observed in those with both vaccination and previous infection.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Dayana Benchoam, Ernesto Cuevasanta, Jonathan A. Semelak, Mauricio Mastrogiovanni, Dario A. Estrin, Matias N. Moller, Beatriz Alvarez
Summary: It has been shown that proteins and peptides can release sulfur under alkaline conditions through the beta-elimination of disulfides, leading to the formation of persulfides and dehydroalanine derivatives. This study examined the formation of glutathione persulfide (GSSH/GSS-) from glutathione disulfide (GSSG) under alkaline conditions. The reaction between GSSG and HO- was found to have a second-order rate constant of approximately 10(-3) M-1 s(-1) at 25 degrees C. The formation of GSSH and the dehydroalanine derivative was confirmed using HPLC and/or mass spectrometry. However, the mixtures did not reach equilibrium within hours, and additional sulfur compounds, including thiol and diverse sulfane sulfur compounds, were also formed. These results caution against overestimating the amount of sulfane sulfur compounds in samples containing disulfides when using cold cyanolysis for quantification. Overall, this study highlights the potential source of persulfides from the beta-elimination of disulfides and emphasizes the need for caution in cold cyanolysis experiments.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)