Article
Environmental Sciences
Ioannis Konstantinidis, Joana P. Firmino, Alberto Ruiz, Bruno Inarra, David San Martin, Alicia Estevez, Jorge M. O. Fernandes, Enric Gisbert
Summary: This study evaluated the replacement of dietary protein sources with Brewer's spent dry yeast (SDY) in gilthead sea bream and found that SDY had a modulatory effect on the host's immunity, oxygen carrier capacity, steroidogenesis, metabolism, and digestion. The results indicate that SDY can be considered as both a sustainable raw material and a functional ingredient.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Ecology
Grazia Marina Quero, Roberta Piredda, Marco Basili, Giulia Maricchiolo, Simone Mirto, Elena Manini, Anne Mette Seyfarth, Marco Candela, Gian Marco Luna
Summary: In this study, the microbiome of farmed seabream was explored using 16S rRNA gene high-throughput sequencing. It was found that the microbiomes associated with different tissues of seabream were distinct from the surrounding environment. The gut microbiome was the most diverse, while the gill and skin microbiomes showed higher similarity to the environmental microbiome. The connections between microbiomes were relatively low among the host's tissues, but the gill and skin microbiomes were found to be the most connected. These findings suggest that the host is the primary driver shaping the seabream microbiome.
Article
Fisheries
A. Toffan, L. Biasini, T. Pretto, M. Abbadi, A. Buratin, R. Franch, G. Dalla Rovere, V. M. Panzarin, A. Marsella, L. Bargelloni, F. Pascoli
Summary: The study revealed that gilthead sea bream larvae are susceptible to RGNNV/SJNNV infection regardless of age, with clear clinical signs and mortality observed only in the youngest group. Survivors tested negative for specific antibodies. The replication efficiency of RGNNV/SJNNV was higher in 21-day-old larvae, correlating with disease outcomes.
Article
Green & Sustainable Science & Technology
Athina Ntzimani, Ioanna Semenoglou, Efimia Dermesonlouoglou, Theofania Tsironi, Petros Taoukis
Summary: This study systematically evaluated the effect of different washing media on microbial load, quality, and shelf life of fish. The results showed that washing with citric acid and peracetic acid significantly delayed the growth of spoilage microorganisms and extended the shelf life of fish.
Article
Agriculture, Dairy & Animal Science
Eleni Mente, Chris G. Carter, Robin S. (Katersky) Barnes, Nikolaos Vlahos, Ioannis Nengas
Summary: Using combinations of plant protein concentrates and EAA supplementation, high levels of replacement (50-75% of fishmeal protein) can be achieved in gilthead sea bream without affecting growth performance or quality traits. It was confirmed that 16% replacement of marine protein with plant protein meets the amino acid needs of sea bream. Further investigation is needed to understand tissue-specific and species-specific responses in regulating metabolism due to dietary nutrient utilization.
Article
Fisheries
Serena Savoca, Miriam Abbadi, Anna Toffan, Cristian Salogni, Carmelo Iaria, Fabiano Capparucci, Rosita Quartesan, Giovanni Loris Alborali, Salvatore Guarnera, Giuseppe Cangemi, Fabio Marino
Summary: This study describes the first occurrence of VNN and LE co-occurring, which synergistically or simultaneously led to a mortality outbreak of Sparus aurata larvae. The samples collected from the cultured gilthead sea bream underwent histopathological, bacteriological, and virological analyses, identifying RGNNV/SJNNV reassortant betanodavirus and Vibrio alginolyticus.
Article
Cell Biology
Isabel Garcia-Perez, Anna Molsosa-Solanas, Miquel Perello-Amoros, Elena Sarropoulou, Josefina Blasco, Joaquim Gutierrez, Daniel Garcia de la Serrana
Summary: This study represents the first characterization of lncRNAs in the gilthead sea bream fast skeletal muscle and provides evidence regarding their involvement in muscle development.
Article
Fisheries
Patricia Pereiro, Antonio Figueras, Beatriz Novoa
Summary: In this study, the immune mechanisms involved in the resistance of Gilthead sea bream to RGNNV infection were elucidated through RNA-Seq analysis. The results showed that sea bream displayed a strong immune response characterized by the high expression of type I interferon-related genes, immune receptors, and antigen presentation-related genes in both brain and head kidney tissues. Additionally, complement-, coagulation-, and angiogenesis-related genes were highly enriched in the head kidney at the earlier sampling point. Interestingly, a neuroprotective scenario with restrained inflammation was observed in the brain of sea bream, unlike the activation of the stress axis and immunosuppression found in sea bass.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Endocrinology & Metabolism
Albert Sanchez-Moya, Sara Balbuena-Pecino, Emilio J. Velez, Miquel Perello-Amoros, Irene Garcia-Meilan, Ramon Fontanillas, Josep Alvar Calduch-Giner, Jaume Perez-Sanchez, Jaume Fernandez-Borras, Josefina Blasco, Joaquin Gutierrez
Summary: This study investigated the effects of cysteamine (CSH) inclusion in fish feed on the growth and GH/IGF-1 axis of gilthead sea bream. Results showed that CSH significantly improved growth performance and modulated the GH/IGF-1 axis through different synergic ways. This suggests that CSH is a feasible candidate to be included in fish feed.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Fisheries
Serena Busti, Alessio Bonaldo, Alessia Diana, Simone Perfetti, Cinzia Viroli, Ramon Fontanillas, Tommy Berger Eriksen, Pier Paolo Gatta, Luca Parma
Summary: This study evaluated the effects of different pellet sizes on the growth, gut evacuation, feed waste, and feed digestibility of gilthead sea bream. The results showed that pellet size did not significantly affect the growth and feed efficiency of the fish, but did have an impact on gastric evacuation rate and feed waste.
Article
Chemistry, Medicinal
Beatriz de la Fuente, Noelia Pallares, Houda Berrada, Francisco J. Barba
Summary: In this study, PLE technique was used for the first time to extract protein extracts with antioxidant activity from side streams of gilthead sea bream, increasing the protein recovery percentages and antioxidant capacity of the extracts. Through ICP-MS and LC-ESI-qTOF-MS analysis, levels of toxic metals were found to be below established limits, with no mycotoxins detected in the sea bream side streams, demonstrating the potential of environmentally friendly technology for valorizing fish processing side streams.
Article
Fisheries
Dimitra Kogiannou, George Rigos
Summary: This study found that the depletion of PZQ from gilthead sea bream tissues was rapid, decreasing to undetectable levels within 24 hours post-treatment. The levels fell below the detection limit in approximately 75 days.
MEDITERRANEAN MARINE SCIENCE
(2021)
Article
Fisheries
Luca Bargelloni, Oronzo Tassiello, Massimiliano Babbucci, Serena Ferraresso, Rafaella Franch, Ludovica Montanucci, Paolo Carnier
Summary: This study identified eight novel genetic loci for resistance to photobacteriosis in gilthead sea bream and revealed a highly polygenic genetic architecture. Machine learning methods, particularly linear bagging, showed higher prediction performance compared to Bayesian regression approaches, indicating that exploring these methods in parallel could increase the chances of highly effective genomic prediction.
AQUACULTURE REPORTS
(2021)
Article
Environmental Sciences
Federico Moroni, Fernando Naya-Catala, M. Carla Piazzon, Simona Rimoldi, Josep Calduch-Giner, Alberto Giardini, Ines Martinez, Fabio Brambilla, Jaume Perez-Sanchez, Genciana Terova
Summary: The addition of nisin-producing Lactococcus lactis in gilthead sea bream had effects on growth performance, transcriptional response, and microbiota, without colonization in the host's intestinal mucosa. The probiotic in the diets modulated the gut microbiota of fish, leading to host modifications without necessarily colonizing the intestine mucosa.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Fisheries
R. Magalhaes, N. Martins, F. Fontinha, S. Moutinho, R. E. Olsen, H. Peres, A. Oliva-Teles
Summary: The study found limited interactions between arachidonic acid (ARA) or docosahexaenoic acid (DHA) and dietary digestible carbohydrates, while the starch content in the diet had a certain impact on the growth performance and feed utilization of gilthead sea bream.
Article
Fisheries
H. Peres, J. M. A. Freitas, P. L. P. Carvalho, W. M. Furuya, M. M. P. Satori, A. Oliva-Teles, L. E. Pezzato, M. M. Barros
Summary: The study revealed that a dietary DP/DE ratio of 16.5-17.2 mg kJ(-1) promoted the maximum growth performance of Nile tilapia juveniles. With an increase in dietary DP/DE ratio, feed intake and energy retention decreased linearly, while feed efficiency increased linearly.
Article
Fisheries
R. Magalhaes, N. Martins, F. Fontinha, A. Couto, C. R. Serra, R. A. Santos, E. Olsen, H. Peres, A. Oliva-Teles
Summary: The present study investigates the effects of different diet compositions on the oxidative status, histomorphology of liver and intestine, and intestinal microbiota modulation in gilthead sea bream juveniles. The results show that ARA-rich diets reduce liver and intestine lipid peroxidation, while DHA-rich diets increase the activity of antioxidant enzymes in the liver. Additionally, dietary starch improves the oxidative stress index in the liver and has positive effects on glutathione and lipid peroxidation in the intestine. Fish fed high starch diets also experience hepatocyte hypertrophy and alterations in nuclei position. Compared to DHA diets, ARA diets result in reduced diversity of intestinal microbiota. In conclusion, high dietary ARA provides protection to the liver and intestine of gilthead sea bream juveniles, and the balanced ratio of ARA/DHA in the diet has important effects on antioxidant status and intestinal microbiota diversity.
Article
Fisheries
Catarina Basto-Silva, Paula Enes, Aires Oliva-Teles, Encarnacion Capilla, Ines Guerreiro
Summary: This study evaluated the effects of dietary protein/carbohydrate ratio and feeding frequency on the growth, metabolism, and economic efficiency of gilthead seabream juveniles. The results showed that feeding a diet with a protein/carbohydrate ratio of 40%/20% twice a day was the most suitable strategy, as it improved fish growth and economic efficiency.
Article
Fisheries
H. Fernandes, C. Castro, D. Filipe, P. Ferreira, J. M. Salgado, F. Moyano, A. Oliva-Teles, I Belo, H. Peres
Summary: The effects of dietary supplementation with brewer's spent grain (BSG) extract on the oxidative status of the liver and intestine, as well as muscle lipid peroxidation (LPO) in European seabass, were investigated. The results showed that BSG extract supplementation increased antioxidant levels in the diet, improved intestinal antioxidant capacity, and reduced muscle LPO in the fish.
AQUACULTURE NUTRITION
(2022)
Article
Fisheries
Catarina Basto-Silva, Claudia R. Serra, Carolina Castro, Guilherme S. Novoa, Aires Oliva-Teles, Encarnacion Capilla, Ines Guerreiro
Summary: This study evaluated the effects of feeding frequency and dietary protein/carbohydrate ratios on intestinal histomorphology, microbiota profile, and digestive and oxidative stress-related enzyme activities of gilthead seabream. The results showed that feeding frequency and diet composition had no significant effects on intestinal histomorphology and microbiota, but influenced the activity of digestive enzymes. It is recommended to feed gilthead seabream juveniles with a diet containing 40% protein and 20% carbohydrates, twice a day.
AQUACULTURE NUTRITION
(2022)
Article
Fisheries
F. Docando, N. Nunez-Ortiz, C. R. Serra, P. Arense, P. Enes, A. Oliva-Teles, P. Diaz-Rosales, C. Tafalla
Summary: This study investigates the effects of Bacillus subtilis on the immune response of rainbow trout. The results show that the ABP1 strain has the potential to enhance the immune response of trout and can be used in designing novel oral vaccination strategies for aquaculture.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
M. Monteiro, P. Perdiguero, A. Couto, C. R. Serra, P. Pereiro, B. Novoa, A. Figueras, L. Ribeiro, P. Pousao-Ferreira, C. Tafalla, A. Oliva-Teles, P. Enes, C. J. Secombes, P. Diaz-Rosales
Summary: Meagre, a potential candidate for Mediterranean aquaculture diversification, has understudied physiological consequences of immune system activation. This study evaluated the in vivo effects of lipo-polysaccharide on the spleen transcriptome of meagre using RNA-seq analysis.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Microbiology
Fabio Rangel, Paula Enes, Laura Gasco, Francesco Gai, Bela Hausmann, David Berry, Aires Oliva-Teles, Claudia R. Serra, Fatima C. Pereira
Summary: The aquaculture industry is rapidly growing, but reliance on wild fish-based meals for carnivorous fish farming is unsustainable. Insect-based diets show promise as fishmeal substitutes, but their impact on farmed fish gut microbiota remains inconclusive and research on microbial functions is scarce.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Felix Docando, Noelia Nunez-Ortiz, Gabriela Goncalves, Claudia R. Serra, Eduardo Gomez-Casado, Diana Martin, Beatriz Abos, Aires Oliva-Teles, Carolina Tafalla, Patricia Diaz-Rosales
Summary: Bacillus subtilis has been proven to be an effective probiotic for aquacultured species, benefiting water quality, fish growth, and immune status. In this study, B. subtilis spores were used as delivery vehicles for the VP2 protein from infectious pancreatic necrosis virus (IPNV). The results showed that the VP2-expressing strain (CRS208) retained its immunomodulatory potential and induced a significant antibody response, making it a promising vaccination strategy.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jorge Matinha-Cardoso, Filipe Coutinho, Steeve Lima, Ana Eufrasio, Antonio Paulo Carvalho, Aires Oliva-Teles, Jose Bessa, Paula Tamagnini, Claudia R. Serra, Paulo Oliveira
Summary: Aquaculture is rapidly growing to meet consumer demands. A new study tested the use of cyanobacterial extracellular vesicles as a novel nanocarrier system for fish, showing potential for biotechnological applications in carrying proteins/enzymes to improve fish health.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Helena Fernandes, Jose Manuel Salgado, Marta Ferreira, Martina Vrsanska, Nelson Fernandes, Carolina Castro, Aires Oliva-Teles, Helena Peres, Isabel Belo
Summary: In this study, Brewer's spent grain (BSG) was fermented with Aspergillus ibericus to produce a crude extract rich in cellulase and xylanase. The fermented BSG showed increased protein content and reduced fiber content, making it a potential supplement in aquafeeds. The crude extract was also found to significantly improve feed and protein utilization in European seabass juveniles.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Nicole Martins, Carolina Castro, Aires Oliva-Teles, Helena Peres
Summary: This study evaluated the effects of feeding and feed deprivation on the regulation of feed intake in European seabass. The results showed that European seabass possess feed intake regulation mechanisms similar to those found in mammals and fish. Different fasting and feeding conditions induce different responses in feed intake regulation mechanisms in European seabass.
Article
Fisheries
Diogo Filipe, Mario Dias, Rui Magalhaes, Helena Fernandes, Jose Salgado, Isabel Belo, Aires Oliva-Teles, Helena Peres
Summary: Aquaculture requires new, economical, and eco-friendly protein sources to replace traditional fisheries and plant ingredients. Using agriculture by-products as protein sources would reduce land-based feed production pressure and waste production, promoting a circular economy and sustainable aquaculture.
Article
Fisheries
Claudia Teixeira, Rita Pedrosa, Carolina Castro, Rui Magalhaes, Elisabete Matos, Aires Oliva-Teles, Helena Peres, Amalia Perez-Jimenez
Summary: This study aimed to assess the potential role of dietary tryptophan supplementation in meagre juveniles in order to minimize the deleterious effect of potential stress conditions. The results indicated that tryptophan supplementation had a negative impact on the growth and feed utilization of the fish, but did not affect these parameters at doses below 0.5%.
Article
Fisheries
Catarina Basto-Silva, Irene Garcia-Meilan, Ana Couto, Claudia R. Serra, Paula Enes, Aires Oliva-Teles, Encarnacion Capilla, Ines Guerreiro
Summary: This study investigated the effects of dietary protein source and protein/carbohydrate ratio on gilthead seabream gut. It was found that plant feedstuff led to more histomorphological alterations, while the P/CH ratio had little effect on gut parameters.
Article
Agriculture, Dairy & Animal Science
Telisson Ribeiro Goncalves, Jose Renaldo Vilar da Silva Filho, Gilmar Amaro Pereira, Tarcisio Martins Meira, Moema Kelly Nogueira de Sa, Glayciane Costa Gois, Crislane de Souza Silva, Fleming Sena Campos, Sandra Mari Yamamoto, Gherman Garcia Leal de Araujo
Summary: This study evaluated the effects of diets containing vitiviniculture waste on intake, digestibility, water balance, nitrogen balance, ingestive behavior, performance, and carcass traits of lambs. The results showed that feeding lambs with diets containing 216 g/kg cactus pear and 144 g/kg vitiviniculture waste resulted in higher intake and digestibility.
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2024)
Article
Agriculture, Dairy & Animal Science
Q. Zhang, C. L. Walk, A. J. Cowieson, K. Stamatopoulos, J. L. Wu, J. O. B. Sorbara
Summary: This study evaluated the efficacy of a novel phytase in terms of phytate hydrolysis and the digestibility of phosphorus and calcium in broiler chickens fed low and high phytate diets. The results showed that the phytase effectively hydrolyzed phytate and increased digestible phosphorus and calcium. The response to dietary phytate varied in terms of age and the specific nutrient being evaluated.
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2024)
Article
Agriculture, Dairy & Animal Science
J. Song, D. M. Holanda, C. H. Heuer, C. M. Nyachoti
Summary: This study determined the energy contents of hybrid rye fed to growing pigs, and investigated the effects of particle size on energy values. Results showed that finely ground hybrid rye had higher digestible energy and metabolizable energy compared to coarsely ground hybrid rye, while net energy only showed a slight improvement.
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2024)
Article
Agriculture, Dairy & Animal Science
Mohamed Salah Ayyat, Ahmed Mohamed Nabil Ayyat, Mohamed S. Abdel-Rahman, Adham A. Al-Sagheer
Summary: This study evaluated the impact of replacing fish meal with sugar beet and carrot leaf protein concentrate in Nile tilapia. The results showed that replacing 75% of fish meal with leaf protein concentrate had no adverse effects on growth and health, and it was more efficient in terms of economic benefits.
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2024)