Article
Biochemistry & Molecular Biology
Veronica A. Cochrane, Zhongying Yang, Mark L. Dell'Acqua, Show-Ling Shyng
Summary: The study revealed that leptin regulates the trafficking of K-ATP channels in pancreatic beta-cells by affecting PKA activity. Increased PKA activity depends on NMDA receptors, CaMKK beta, and AMPK, and is anchored to AKAP79/150 at the cell membrane. disrupting AKAP79/150 coordination may provide a potential target for restoring insulin secretion in beta-cells with defective leptin signaling.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Duangnapa Kovanich, Teck Yew Low, Manuela Zaccolo
Summary: cAMP is a second messenger that plays a crucial role in regulating cellular functions. The compartmentalization of cAMP signaling is essential for its specificity, and the formation of dynamic signaling domains is responsible for precise spatiotemporal regulation. Proteomics can be used to identify the components of these domains and define the dynamic landscape of cellular cAMP signaling. Understanding compartmentalized cAMP signaling in physiological and pathological conditions can provide insights into disease mechanisms and guide the development of precision medicine interventions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Mitsumasa Koyanagi, Baoguo Shen, Takashi Nagata, Lanfang Sun, Seiji Wada, Satomi Kamimura, Eriko Kage-Nakadai, Akihisa Terakita
Summary: Optical control of GPCR signaling is a valuable approach for understanding and controlling GPCR-based physiologies. In this study, the performance of two bistable opsins, MosOpn3 and LamPP, in optical control in vivo was systematically investigated using Caenorhabditis elegans. The results demonstrated the advantages of MosOpn3 and LamPP in light-induced behavior and color-dependent control of behavior, respectively. Molecular engineering was also applied to enhance the usability of these opsins. These findings provide numerous strategies for optical control of GPCR-based physiologies and GPCR signaling.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Eshan Sharma, Akanksha Bhatnagar, Avantika Bhaskar, Susmita M. Majee, Martin Kieffer, Stefan Kepinski, Paramjit Khurana, Jitendra P. Khurana
Summary: The rice FB protein-coding gene OsFBX257 is differentially expressed under drought conditions and other abiotic stresses. It plays a significant role in modulating root architecture and improving drought stress tolerance in rice. OsFBX257 is a potential breeding target for alleviating drought stress-induced damage in rice.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Peng Liu, Fuqiang Zu, Hui Chen, Xiaoli Yin, Xiaodong Tan
Summary: This study revealed that exosomal DNAJB11 promotes cell development in pancreatic cancer cells by regulating the expression of HSPA5 and activating the endoplasmic reticulum stress pathway. Additionally, exosomal DNAJB11 can regulate the expression of EGFR and activate the MAPK signaling pathway. Clinical studies suggest that exosomal DNAJB11 has the potential to serve as a diagnostic biomarker and therapeutic target for pancreatic cancer.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2022)
Review
Endocrinology & Metabolism
Morris F. White, C. Ronald Kahn
Summary: The discovery and application of insulin marked a major turning point in medical history, playing a crucial role in blood glucose regulation and the treatment of diseases like diabetes.
MOLECULAR METABOLISM
(2021)
Article
Multidisciplinary Sciences
Liliana Felicia Iannucci, Anna Maria D'Erchia, Ernesto Picardi, Daniela Bettio, Filippo Conca, Nicoletta Concetta Surdo, Giulietta Di Benedetto, Deborah Musso, Cristina Arrigoni, Marco Lolicato, Mauro Vismara, Francesca Grisan, Leonardo Salviati, Luciano Milanesi, Graziano Pesole, Konstantinos Lefkimmiatis
Summary: The study reveals an unexpected mechanism by which EPAC1 enters the nucleus and regulates the transcription of a histone cluster by forming biomolecular condensates in its proximity in response to cAMP.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Mariam Ahmed Galal, Samhar Samer Alouch, Buthainah Saad Alsultan, Huda Dahman, Nouf Abdullah Alyabis, Sarah Ammar Alammar, Ahmad Aljada
Summary: This comprehensive review thoroughly explores the intricate involvement of insulin receptor isoforms and insulin-like growth factor receptors in the insulin and insulin-like growth factor signaling pathway, emphasizing their crucial roles in cancer development and resistance to anti-cancer drugs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Paul Gehrtz, Shir Marom, Mike Buehrmann, Julia Hardick, Silke Kleinboelting, Amit Shraga, Christian Dubiella, Ronen Gabizon, Jan N. Wiese, Matthias P. Mueller, Galit Cohen, Ilana Babaev, Khriesto Shurrush, Liat Avram, Efrat Resnick, Haim Barr, Daniel Rauh, Nir London
Summary: High-throughput nanomole-scale synthesis allows for efficient and economical late-stage functionalization of compounds. Copper-catalyzed azide-alkyne cycloaddition has been demonstrated to be capable of nanoscale late-stage functionalization of covalent kinase inhibitors, enabling the synthesis of hundreds of compounds that do not require purification for biological assay screening. This study presents a promising approach to improving the properties of lead chemical matter through high-throughput late-stage functionalization of covalent inhibitors.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Review
Pharmacology & Pharmacy
Lokender Kumar, Whitney Vizgaudis, Judith Klein-Seetharaman
Summary: The insulin receptor plays a vital role in regulating nutrient balance and is an attractive target for diabetes and metabolic syndrome treatment. This study investigates the pharmacology of the insulin receptor through two mechanisms: activation by insulin mimetics and inhibition of its enzymatic activity. Recent progress in cryoelectron microscopy allows for a detailed understanding of the initial insulin ligand binding events. The researchers conducted a comprehensive docking study to gain structural insights into the insulin receptor pharmacology.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Roberto Maggio, Irene Fasciani, Marco Carli, Francesco Petragnano, Francesco Marampon, Mario Rossi, Marco Scarselli
Summary: The passage discusses the importance of information exchange and interpretation in biology, particularly in how cells integrate signals from various molecules. G protein-coupled receptors (GPCRs) play a crucial role in signal recognition and transduction within cells, and their formation of higher order oligomers helps organize cell compartments along the membrane. This structural basis allows for cells to perceive and discriminate localized increases in second messengers, contributing to the understanding of physiological roles played by receptor homo- and hetero-oligomerization in cell biology.
Article
Multidisciplinary Sciences
Jun Xu, Nobuo Koizumi, Yusuke V. Morimoto, Ryo Ozuru, Toshiyuki Masuzawa, Shuichi Nakamura
Summary: Nucleotide second messengers play crucial roles in signal transduction in various organisms. A study has found that the swimming motility of the soil bacterium Leptospira kobayashii can be rapidly modulated by light stimulation. Through analysis of a loss-of-photoresponsivity mutant, a novel sensory gene was identified, and the light-dependent synthesis of cyclic adenosine monophosphate (cAMP) was elucidated. These findings reveal a new role for cAMP in controlling the flagella-dependent motility of Leptospira and highlight the global distribution of the newly discovered photoactivated cyclase among diverse microbial species.
SCIENTIFIC REPORTS
(2022)
Review
Endocrinology & Metabolism
Thi Kim Chung Le, Xuan Dat Dao, Dang Vung Nguyen, Duc Huy Luu, Thi Minh Hanh Bui, Thi Huong Le, Huu Thang Nguyen, Tran Ngoan Le, Toshio Hosaka, Thi Thu Thao Nguyen
Summary: The discovery of insulin in 1921 sparked research on insulin activity and insulin resistance. This mini-review synthesizes updated discoveries in this field to uncover related mechanisms and inform the development of novel applications.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Iain C. Clark, Bruk Mensa, Christopher J. Ochs, Nathan W. Schmidt, Marco Mravic, Francisco J. Quintana, William F. DeGrado, Adam R. Abate
Summary: Using a hybrid valve- and droplet-based microfluidic system, a combinatorial DNA library encoding an engineered histidine kinase based on bacterial CpxA was successfully constructed. Surprising results were found, such as the different effects on catalytic activity caused by the combinations of the S helix and TM domain. It was concluded that the intervening HAMP domain passively transmits signals and shapes the signaling response depending on subtle changes in neighboring domains, leading to a richness in functional outputs as HKs vary in response to changing evolutionary pressures.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Endocrinology & Metabolism
E. Dale Abel
Summary: Insulin receptors play a critical role in regulating cardiac growth, survival, substrate uptake, and mitochondrial metabolism. Altered insulin signaling in the heart can contribute to ventricular remodeling and heart failure progression. Understanding the role of insulin signaling in cardiac physiology and pathology may have therapeutic implications for conditions like obesity and diabetes.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2021)
Article
Biochemical Research Methods
Karidia Konate, Marion Dussot, Gudrun Aldrian, Anais Vaissiere, Veronique Viguier, Isabel Ferreiro Neira, Franck Couillaud, Eric Vives, Prisca Boisguerin, Sebastien Deshayes
BIOCONJUGATE CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Saher Rahmani, Jelena Budimir, Mylene Sejalon, Morgane Daurat, Dina Aggad, Eric Vives, Laurence Raehm, Marcel Garcia, Laure Lichon, Magali Gary-Bobo, Jean-Olivier Durand, Clarence Charnay
Article
Immunology
Malika Moussouni, Pauline Nogaret, Preeti Garai, Berengere Ize, Eric Vives, Anne-Beatrice Blanc-Potard
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2019)
Article
Multidisciplinary Sciences
Mariana Rosas Olvera, Preeti Garai, Gregoire Mongin, Eric Vives, Laila Gannoun-Zaki, Anne-Beatrice Blanc-Potard
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Sebastien Deshayes, Karidia Konate, Marion Dussot, Berengere Chavey, Anais Vaissiere, Thi Nhu Ngoc Van, Gudrun Aldrian, Kart Padari, Margus Pooga, Eric Vives, Prisca Boisguerin
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2020)
Article
Multidisciplinary Sciences
Karidia Konate, Quentin Seisel, Eric Vives, Prisca Boisguerin, Sebastien Deshayes
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2020)
Article
Pharmacology & Pharmacy
Isabel Ferreiro, Coralie Genevois, Karidia Konate, Eric Vives, Prisca Boisguerin, Sebastien Deshayes, Franck Couillaud
Summary: The study demonstrates the in vivo efficiency of WRAP5:siRNA nanoparticles delivering siRNA to cancer cells in solid tumors, achieving specific gene silencing through RNA interference and significantly downregulating the expression of firefly luciferase, with no toxicity and sustained presence in the tumor for at least 10 days.
Article
Polymer Science
Ruben Faria, Eric Vives, Prisca Boisguerin, Angela Sousa, Diana Costa
Summary: Mitochondria are cellular organelles that produce cellular energy in the form of ATP. Mutations in the mitochondrial DNA can lead to various disorders, prompting the exploration of gene therapy as a promising treatment approach. Targeting mitochondria using mitochondrial DNA and peptide-based complexes shows potential for restoring normal mitochondrial function and advancing mitochondrial gene therapy.
Review
Biochemistry & Molecular Biology
Prisca Boisguerin, Karidia Konate, Emilie Josse, Eric Vives, Sebastien Deshayes
Summary: Gene therapy offers the potential to address faulty genes by delivering therapeutic nucleic acids or stimulating the immune system, but the delivery of these exogenous nucleic acids remains a challenge. Cell-penetrating peptides are gaining interest as delivery systems for their ability to form a variety of nanocomplexes, with common features including defined formulation conditions for optimal delivery.
Article
Pharmacology & Pharmacy
Quentin Seisel, Israpong Lakumpa, Emilie Josse, Eric Vives, Jessica Varilh, Magali Taulan-Cadars, Prisca Boisguerin
Summary: Therapeutic peptides have regained interest as they can address unmet medical needs and can be an excellent complement to pharmaceutic small molecules and other macromolecular therapeutics. In this study, the optimization of a peptidyl inhibitor and the screening of cell-penetrating peptides resulted in the identification of an optimal peptide for CFTR stabilization. Using this peptide, an additive increase in CFTR amount was observed when combined with a small-molecule treatment. This finding is significant for the development of CFTR-stabilizing peptides as additional treatments for CF patients.
Article
Pharmacology & Pharmacy
Ruben Faria, Milan Paul, Swati Biswas, Eric Vives, Prisca Boisguerin, Angela Sousa, Diana Costa
Summary: Mitochondria have their own genome and mutations in mitochondrial DNA can lead to various diseases. Developing a gene delivery system specifically targeting mitochondria is crucial for mitochondrial gene therapy. This study compared polymer- and peptide-based systems and found that peptides have a better capability to complex with DNA, forming smaller particles suitable for cellular transfection and mitochondrial gene therapy.
Article
Pharmacology & Pharmacy
Ana Raquel Neves, Tania Albuquerque, Ruben Faria, Ana M. Goncalves, Cecilia Santos, Eric Vives, Prisca Boisguerin, Luis A. Passarinha, Angela Sousa, Diana Costa
Summary: Despite progress in cancer diagnosis and treatment, glioblastoma remains the most lethal brain tumor. Gene therapy focused on non-viral vectors, such as transferrin (Tf), has been developed as a promising approach. In this study, a cell-penetrating peptide (WRAP5) targeting Tf was used to condense tumor suppressor p53-encoding plasmid DNA (pDNA), forming nanocomplexes. These complexes showed efficient cellular internalization, gene expression, and decreased viability of glioblastoma cells.
Article
Cell & Tissue Engineering
Gautier Tejedor, Prisca Boisguerin, Eric Vives, Christian Jorgensen, Jerome Guicheux, Claire Vinatier, Claire Gondeau, Farida Djouad
Summary: An interfering peptide was designed and synthesized to enhance the immunoregulatory properties of mesenchymal stem cells.
STEM CELLS INTERNATIONAL
(2022)
Article
Multidisciplinary Sciences
Jona Karam, Fabien P. Blanchet, Eric Vives, Prisca Boisguerin, Yves-Marie Boudehen, Laurent Kremer, Wassim Daher
Summary: It has been discovered that neutralizing anti-CD81 antibodies and deletion of the large extracellular loop (LEL) of CD81 significantly reduce the uptake of Mab by macrophages. Saturation of Mab with soluble GST-CD81-LEL or CD81-LEL-derived peptides also decreases the internalization of the bacteria. The study unveils AhpC as a major interactant of CD81-LEL, and pre-exposure of macrophages with soluble AhpC inhibits mycobacterial uptake while overexpression of AhpC in Mab enhances its internalization. These findings highlight the previously unexplored role of CD81/AhpC in promoting the uptake of pathogenic mycobacteria by host cells.
Meeting Abstract
Biochemistry & Molecular Biology
Eric Vives, Karidia Konate, Gudrun Aldrian, Sebastien Deshayes, Prisca Boisguerin
JOURNAL OF PEPTIDE SCIENCE
(2018)