Article
Immunology
Lixia Gao, Jie Yang, Jutao Feng, Ziying Liu, Ying Dong, Jiangyan Luo, Liangzhentian Yu, Jiamei Wang, Hongying Fan, Weifeng Ma, Tiancai Liu
Summary: This study developed a novel strategy to suppress HBV DNA replication and eliminate cccDNA by preparing biomimetic nanoparticles with high targeting ability. The nanoparticles showed excellent stability and safety property, and exhibited significant inhibitory effects on various CHB-related markers.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Shuang Yang, Dandan Wang, Xia Zhang, Yaojun Sun, Bin Zheng
Summary: The study designed and prepared a novel siRNA delivery system, cRGD-PSH-NP, which showed excellent gene silencing and antitumor activity in HepG2 cells. In vivo, cRGD-PSH-NP/S demonstrated potent tumor inhibition in HepG2-bearing nude mice without inducing toxicity, suggesting further research potential in hepatocarcinoma therapy.
Article
Medicine, Research & Experimental
Pranjita Zantye, Soniya Shende, Sutapa Roy Ramanan, Indrani Talukdar, Meenal Kowshik
Summary: The study demonstrated the synthesis of self-fluorescent, cell-penetrating peptide-functionalized hydroxyapatite nanoparticles (R8HNPs) for RNA interference delivery in stem cells, showing good biocompatibility and delivery efficiency, achieving 80% silencing of the pluripotency marker gene Oct4 in R1 ESCs.
MOLECULAR PHARMACEUTICS
(2021)
Article
Chemistry, Multidisciplinary
Jinbang Zhang, Jingwan Han, Hui Li, Zhengyang Li, Pengfei Zou, Jiaxin Li, Te Zhao, Junwei Che, Yang Yang, Meiyan Yang, Yuli Wang, Wei Gong, Zhiping Li, Lin Li, Chunsheng Gao, Haihua Xiao
Summary: A new system is constructed by functionalizing siRNA delivery lipid nanoparticles with the lymphocyte membrane and 12p1, which can escape immune recognition, has HIV-1 neutralizing capacity, and the ability to deliver siRNA specifically into HIV-1-infected cells. This system can escape uptake by the mononuclear phagocyte system, exhibits strong binding ability with gp120, and shows significant gene silencing effect on HIV-1-infected cells.
Article
Chemistry, Physical
Josepha Roerig, Franziska Mitrach, Maximilian Schmid, Gerd Hause, Michael C. Hacker, Christian Woelk, Michaela Schulz-Siegmund
Summary: This study explores innovative exogenous loading strategies for small interfering RNA (siRNA) to enhance the drug loading capacity of milk-derived extracellular vesicles (EVs). Two loading strategies are compared experimentally, and it is found that combining stabilized calcium phosphate nanoparticles (CaP-NP) and siRNA pre-accumulated in liposomes leads to a synergistic loading performance.
Article
Chemistry, Multidisciplinary
Runze Li, Xiaohui Wu, Jing Li, Xuehe Lu, Robert Chunhua Zhao, Jianbing Liu, Baoquan Ding
Summary: This study presents a nanoplatform based on branched DNA aptamer clusters for efficient targeted drug delivery. The covalently conjugated branched DNA aptamer cluster-modified DNA tetrahedron demonstrates highly targeted cellular uptake, resulting in selective inhibition of tumor cell proliferation after loading the chemotherapeutic drug.
Article
Chemistry, Multidisciplinary
Chonprakun Thagun, Yoko Horii, Maai Mori, Seiya Fujita, Misato Ohtani, Kousuke Tsuchiya, Yutaka Kodama, Masaki Odahara, Keiji Numata
Summary: This article presents a method for high-throughput nucleic acid delivery in plants using peptide nanocarriers sprayed on the leaf surface. It demonstrates effective delivery of DNA and siRNA molecules into plant cells, allowing for nontransgenic modification of economically important plant traits.
Article
Plant Sciences
Jiaxi Yong, Miaomiao Wu, Run Zhang, Shengnan Bi, Christopher W. G. Mann, Neena Mitter, Bernard J. Carroll, Zhi Ping Xu
Summary: This study demonstrates that layered double hydroxide nanoparticles can quickly penetrate plant leaf cells and chloroplasts, delivering small interfering RNA to downregulate targeted genes. This has significant implications for plant protection against viruses and understanding gene function.
Article
Biochemistry & Molecular Biology
Zhen Shi, Yuhan Yang, Ziyang Guo, Shun Feng, Yu Wan
Summary: In this study, a novel redox- and enzyme-responsive fluorinated polyarginine (PFC-PR) was developed as an anti-cancer siRNA carrier. The PFC-PR showed efficient binding with siRNA and enhanced stability, serum tolerance, cellular uptake, and endosome escape. It also exhibited responsiveness to cancer cell-specific conditions, leading to enhanced siRNA release and cancer-targeting ability. Overall, the PFC-PR demonstrated good biocompatibility and high gene silencing efficiency, making it a potential candidate for anti-cancer gene therapy applications.
BIOORGANIC CHEMISTRY
(2023)
Article
Metallurgy & Metallurgical Engineering
Man-fei Yi, Liang-jian Chen, Hui-li He, Lei Shi, Chun-sheng Shao, Bo Zhang
Summary: Improvements in calcium phosphate nanoparticles carriers can enhance gene transfection efficiency and promote osteoblast differentiation. The Mg-CaPNPs-RGD-TAT-CKIP-1 siRNA carrier system showed enhanced cellular uptake and inducement of osteogenesis.
JOURNAL OF CENTRAL SOUTH UNIVERSITY
(2021)
Article
Nanoscience & Nanotechnology
Seokhwan Chung, Yutaro Sugimoto, Jianxi Huang, Miqin Zhang
Summary: This study demonstrates the use of peptide-functionalized nanoparticles to deliver siRNA to sensitize glioma cells to drugs and achieve efficient gene silencing.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Engineering, Chemical
Jianke Tan, Chengji Xu, Xiaodan Zhang, Yuming Huang
Summary: A new type of defect carbon encapsulated magnetic metallic Co nanoparticles (Co@DC) was prepared and shown to efficiently activate peroxymonosulfate (PMS) for the degradation of dyes. The Co@DC exhibited good magnetism, stability, and activation ability across a wide pH range. The mechanism of PMS activation and degradation pathway of the dye were also investigated.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Medicine, Research & Experimental
Tatyana Torkhovskaya, Lyubov Kostryukova, Yulia A. Tereshkina, Elena G. Tikhonova, Galina E. Morozevich, Anna D. Plutinskaya, Alexey Yu Lupatov, Andrey A. Pankratov
Summary: This study explored the modification of a previously developed delivery system for the photodynamic therapy drug Ce6 by adding cell-penetrating and/or targeting peptides, showing increased cytotoxicity in HepG2 cells. The NGR peptide enhanced the internalization of Ce6 in cells, while the R7 peptide increased its cytotoxic effect.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Review
Biochemistry & Molecular Biology
Samuel A. Wickline, Kirk K. Hou, Hua Pan
Summary: Peptide-based nanoparticles (PBN) are recognized as potent pharmaceutical vehicles for precise control of protein production and gene delivery in extrahepatic diseases. This review summarizes the principles and mechanisms of self-assembled formation of PBN, cellular uptake, endosomal release, and systemic delivery to extrahepatic disease sites. Selected examples of PBN with recent proof of concept in disease models in vivo are presented to provide a comparative view and potential for clinical application in the field.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nanoscience & Nanotechnology
Xia Li, Wenjie Mei, Qiong Wu, Jianjun Wang, Lifeng Qi
Summary: This study presents a novel siRNA delivery nanoplatform using ruthenium polypyridine complex nanoparticles (RPNs). The RPNs loaded with EGFR RNA aptamer and Notch3 siRNA chimera showed superior RNAi effects against Notch3 gene compared to Lipofectamine. Furthermore, the RPN-chimera complexes exhibited significant in vivo antitumor effects against ovarian cancer, indicating great potential for future cancer imaging guided gene therapy.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Laure Bar, Christophe Nguyen, Mathieu Galibert, Francisco Santos-Schneider, Gudrun Aldrian, Jerome Dejeu, Remy Lartia, Liliane Coche-Guerente, Franck Molina, Didier Boturyn
Summary: Antibodies are not only important for clinical diagnostics and biopharmaceutical analysis, but also as drugs for treating various diseases. The identification of antibody-epitope binding sites, particularly for designing monoclonal antibody mimics like rituximab, is crucial for medical and bioanalytical applications. Rituximab, a monoclonal antibody used to treat cancers and autoimmune disorders, binds to targeted cells via the recognition of the CD20 epitope, with its binding area located on the surface of rituximab.
ANALYTICAL CHEMISTRY
(2021)
Article
Materials Science, Multidisciplinary
Pauline Jeanjean, Dounia El Hamrani, Coralie Genevois, Bruno Quesson, Franck Couillaud
Summary: This study presents an integrated technology for assessing in vivo anticancer treatments in mice, using different heating conditions. Bioluminescence imaging is used to evaluate the physiological response of tumor and tumor microenvironment to heat treatment induced by magnetic resonance-guided high-intensity focused ultrasound.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Multidisciplinary Sciences
Juliana Rey, Maike Breiden, Vanda Lux, Anika Bluemke, Maike Steindel, Kamilla Ripkens, Bastian Moellers, Kenny Bravo Rodriguez, Prisca Boisguerin, Rudolf Volkmer, Joel Mieres-Perez, Tim Clausen, Elsa Sanchez-Garcia, Michael Ehrmann
Summary: This study reports a novel and reversible mechanism of protease activation, where an inactive protease serves as the activator. This mechanism involves the formation of an allosteric complex between HTRA1 and calpain 2, and exhibits activity only towards specific conformations of substrates. These findings have important implications for understanding protein quality control and potential side effects of drug modulation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Pharmacology & Pharmacy
Quentin Seisel, Israpong Lakumpa, Emilie Josse, Eric Vives, Jessica Varilh, Magali Taulan-Cadars, Prisca Boisguerin
Summary: Therapeutic peptides have regained interest as they can address unmet medical needs and can be an excellent complement to pharmaceutic small molecules and other macromolecular therapeutics. In this study, the optimization of a peptidyl inhibitor and the screening of cell-penetrating peptides resulted in the identification of an optimal peptide for CFTR stabilization. Using this peptide, an additive increase in CFTR amount was observed when combined with a small-molecule treatment. This finding is significant for the development of CFTR-stabilizing peptides as additional treatments for CF patients.
Article
Pharmacology & Pharmacy
Ruben Faria, Milan Paul, Swati Biswas, Eric Vives, Prisca Boisguerin, Angela Sousa, Diana Costa
Summary: Mitochondria have their own genome and mutations in mitochondrial DNA can lead to various diseases. Developing a gene delivery system specifically targeting mitochondria is crucial for mitochondrial gene therapy. This study compared polymer- and peptide-based systems and found that peptides have a better capability to complex with DNA, forming smaller particles suitable for cellular transfection and mitochondrial gene therapy.
Article
Pharmacology & Pharmacy
Ana Raquel Neves, Tania Albuquerque, Ruben Faria, Ana M. Goncalves, Cecilia Santos, Eric Vives, Prisca Boisguerin, Luis A. Passarinha, Angela Sousa, Diana Costa
Summary: Despite progress in cancer diagnosis and treatment, glioblastoma remains the most lethal brain tumor. Gene therapy focused on non-viral vectors, such as transferrin (Tf), has been developed as a promising approach. In this study, a cell-penetrating peptide (WRAP5) targeting Tf was used to condense tumor suppressor p53-encoding plasmid DNA (pDNA), forming nanocomplexes. These complexes showed efficient cellular internalization, gene expression, and decreased viability of glioblastoma cells.
Article
Biochemistry & Molecular Biology
Zarifa Osmanli, Theo Falgarone, Turkan Samadova, Gudrun Aldrian, Jeremy Leclercq, Ilham Shahmuradov, Andrey Kajava
Summary: Alternative splicing is an important mechanism for generating protein diversity in cells. However, there is still a lack of structural data on alternative protein isoforms, as experimental studies typically focus on canonical proteins. In recent years, advances in bioinformatics tools and the development of the AlphaFold program have allowed for the modeling of high-confidence structures of isoforms. In this study, in silico analysis of 58 eukaryotic proteomes was performed, revealing differences in signal peptides, transmembrane regions, and tandem repeat regions between isoforms and canonical counterparts, potentially impacting protein function and cellular localization.
Article
Cell & Tissue Engineering
Gautier Tejedor, Prisca Boisguerin, Eric Vives, Christian Jorgensen, Jerome Guicheux, Claire Vinatier, Claire Gondeau, Farida Djouad
Summary: An interfering peptide was designed and synthesized to enhance the immunoregulatory properties of mesenchymal stem cells.
STEM CELLS INTERNATIONAL
(2022)
Article
Multidisciplinary Sciences
Jona Karam, Fabien P. Blanchet, Eric Vives, Prisca Boisguerin, Yves-Marie Boudehen, Laurent Kremer, Wassim Daher
Summary: It has been discovered that neutralizing anti-CD81 antibodies and deletion of the large extracellular loop (LEL) of CD81 significantly reduce the uptake of Mab by macrophages. Saturation of Mab with soluble GST-CD81-LEL or CD81-LEL-derived peptides also decreases the internalization of the bacteria. The study unveils AhpC as a major interactant of CD81-LEL, and pre-exposure of macrophages with soluble AhpC inhibits mycobacterial uptake while overexpression of AhpC in Mab enhances its internalization. These findings highlight the previously unexplored role of CD81/AhpC in promoting the uptake of pathogenic mycobacteria by host cells.
Article
Biochemistry & Molecular Biology
Salome Ruiz-Demoulin, Eva Trenquier, Sanaa Dekkar, Sebastien Deshayes, Prisca Boisguerin, Cesar Serrano, Pascal de Santa Barbara, Sandrine Faure
Summary: Gastrointestinal stromal tumor (GIST) is mainly caused by a KIT receptor tyrosine kinase oncogenic mutation. Targeting KIT using tyrosine kinase inhibitors provides benefit, but resistance eventually develops. This study shows that LIX1 is upregulated in response to imatinib or sunitinib treatment in GIST cells and silencing LIX1 enhances the anti-tumor effect of imatinib by inhibiting MAPK signaling reactivation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Ruben Faria, Prisca Boisguerin, Angela Sousa, Diana Costa
Summary: Mitochondria are crucial cellular organelles responsible for energy production in cells, and mutations in their DNA (mtDNA) can lead to various diseases. Mitochondrial gene therapy is a promising strategy to address these disorders. Developing efficient mtDNA-based delivery systems to target and transfect mammalian mitochondria is an exciting area of research that can contribute to restoring normal mitochondrial function.
