Review
Cell Biology
Bethan A. Cole, Esther B. E. Becker
Summary: The TRPC3 channel is a crucial player in calcium signaling and is regulated by various endogenous and exogenous factors. Recent studies have advanced our understanding of the regulatory mechanisms of TRPC3, which has implications for the development of therapeutic interventions for diseases associated with this channel.
Article
Chemistry, Medicinal
Yin-Ying Mak, Bi-Juin Loong, Paul Millns, Claudia C. Bauer, Robin S. Bon, Yvonne Mbaki, Fong-Kai Lee, Kuan-Hon Lim, Cin Kong, Sue-Mian Then, Kang-Nee Ting
Summary: This study investigated the vasorelaxant effects of schwarzinicine A, a newly reported alkaloid from Ficus schwarzii Koord. The results showed that schwarzinicine A exerts its significant vasorelaxant effect by blocking multiple TRPC channels and L-type VGCC.
PHYTOTHERAPY RESEARCH
(2022)
Article
Microbiology
Liping Peng, Jiawen Du, Runfan Zhang, Nali Zhu, He Zhao, Qiang Zhao, Qilin Yu, Mingchun Li
Summary: The study found that the growth and cell vitality of the Δcnb1/Delta/Delta yvc1/Delta/Delta mutant were better than the Δcnb1/Delta/Delta strain. Under ER stress, the cellular calcium concentration decreased and fluctuation was weakened in the Δcnb1/Delta/Delta yvc1/Delta/Delta strain.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lili Chen, Zhuang Zhang, Hongtao Tian, Shan Jiang, Yunyun Ji, Mengru Liu, Jianhua Shen, Zhengyu Cao, Kai Wang
Summary: This study reports the synthesis and biological evaluation of a novel series of benzimidazole-based TRPC5 inhibitors. One compound, 8b, is 100-fold more potent than the parent compound, AC1903, in the suppression of TRPC5 channel activity. Interestingly, both AC1903 and 8b also suppressed TRPC4 channel activity with similar potency. Compound 8b also significantly blunts protamine sulfate-induced reorganization of podocyte cytoskeleton, interleukin (IL)-17-induced cell proliferation, and the expression of proinflammatory mediators in human keratinocyte HaCaT cells.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Cell Biology
Katelyn E. Sadler, Francie Moehring, Stephanie Shiers, Lauren J. Laskowski, Alexander R. Mikesell, Zakary R. Plautz, Allison N. Brezinski, Christina M. Mecca, Gregory Dussor, Theodore J. Price, John D. McCorvy, Cheryl L. Stucky
Summary: The study identified TRPC5 as a key contributor to tactile and spontaneous pain in multiple rodent pain models, particularly in conditions characterized by elevated LPC concentrations. 75% of human sensory neurons express TRPC5, and its activity is directly modulated by LPC.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Immunology
Omar Rafael Aleman, Nancy Mora, Carlos Rosales
Summary: Human neutrophils express two unique antibody receptors for IgG, Fc gamma RIIa and Fc gamma RIIIb, which are capable of initiating distinct cellular functions. Fc gamma RIIa induces an increase in L-selectin expression and phagocytosis, while Fc gamma RIIIb promotes actin polymerization, activation of beta 1 integrins, and formation of neutrophils extracellular traps (NET) more efficiently.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Xiao-Yu Cheng, Shu-Fang Li, Yong Chen, Ying-Jie Zhao, Wei Hu, Chao Lu, Ren-Peng Zhou
Summary: TRPM7 is a channel protein located on the cell membrane that regulates the balance of intracellular Mg2+. It plays an important role in cell proliferation, adhesion, migration, and is directly related to disease occurrence and development.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Review
Chemistry, Medicinal
Yuan-Yuan Gao, Wen Tian, Hui-Nan Zhang, Yang Sun, Jing-Ru Meng, Wei Cao, Xiao-Qiang Li
Summary: TRPCs channels play crucial roles in human physiological and pathological processes, but finding selective TRPCs modulators remains challenging. Further exploration for more effective and selective TRPCs modulators is needed.
ARCHIVES OF PHARMACAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Gerald Thiel, Oliver G. Roessler
Summary: In this study, the functional role of calmodulin in regulating signal transduction of TRPM3 and TRPM8 channels was investigated. It was found that both a calmodulin mutant and calmodulin inhibitor significantly impaired the signaling of TRPM3 and TRPM8. The negative regulation of gene transcription by the calmodulin-dependent protein phosphatase calcineurin was also demonstrated. Calmodulin plays a dual role in regulating the functions of TRPM3 and TRPM8.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Samu Luostarinen, Mari Hamalainen, Eeva Moilanen
Summary: TRPA1 is mainly expressed in sensory neurons where it plays a role in itch, pain, and neurogenic inflammation. Recent studies have shown that nonneuronal cells also express TRPA1 to support inflammatory responses. The expression of TRPA1 in keratinocytes can be induced by the proinflammatory cytokine TNF through NF-kappa B and MAP kinase signaling pathways, and drugs commonly used to treat skin inflammation can decrease TRPA1 expression, indicating a potential drug target for inflammatory skin diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Axel Dietschmann, Sebastian Schruefer, Stefanie Westermann, Fiona Henkel, Kirstin Castiglione, Ralf Willebrand, Jasmin Adam, Jurgen Ruland, Roland Lang, Donald C. Sheppard, Julia Esser-von-Bieren, Daniel Radtke, Sven Krappmann, David Voehringer
Summary: Eosinophilia is associated with various inflammatory diseases, such as allergic bronchopulmonary aspergillosis (ABPA). It has been shown that direct interaction between eosinophils and Aspergillus fumigatus is essential for the activation of eosinophils, which then secrete multiple proinflammatory mediators. The PI3K-AKT-mTOR pathway plays a crucial role in A. fumigatus-induced eosinophil activation.
Article
Oncology
Li-ping Bai, Ya-li Chen, Ai Zheng
Summary: This study found that the high expression of TRPC6 protein in cervical cancer cell lines HeLa and SiHa may be related to the malignant behavior of the cells. SKF-96365 inhibits cell proliferation and DNA synthesis, while OAG promotes cell proliferation and DNA synthesis.
CANCER CELL INTERNATIONAL
(2022)
Review
Biochemistry & Molecular Biology
Omar Mahmoud, Georgia Biazus Soares, Gil Yosipovitch
Summary: Transient Receptor Potential (TRP) channels, found abundantly in the skin, play a crucial role in the sensory pathways of itch, pain, and inflammation. This review article explores the complex physiology of different TRP channels and their involvement in modulating itch sensation and the pathophysiology of acute and chronic itch conditions. The article also highlights emerging small molecule and topical TRP channel agents as potential anti-pruritic treatments, with some advancing into clinical trials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Yiming Zhou, Choah Kim, Juan Lorenzo B. Pablo, Fan Zhang, Ji Yong Jung, Li Xiao, Silvana Bazua-Valenti, Maheswarareddy Emani, Corey R. Hopkins, Astrid Weins, Anna Greka
Summary: The inhibition of TRPC5 channels has been shown to protect podocytes and suppress proteinuria in a puromycin aminonucleoside-induced nephrosis model, suggesting a potential therapeutic strategy for patients with kidney diseases.
FRONTIERS IN MEDICINE
(2021)
Article
Immunology
Gregory R. Tintinger, Annette J. Theron, Helen C. Steel, Moloko C. Cholo, Jan G. Nel, Charles Feldman, Ronald Anderson
Summary: The study investigated the impact of bedaquiline on platelet activation and found that it exerts anti-platelet effects by inhibiting ADP-activated expression of CD62P, aggregation, and Ca2+ mobilization, possibly through its action on PI3-K. These anti-platelet effects may help mitigate the risk of TB-associated cardiovascular disease.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Opeyemi A. Olabisi, Jia-Yue Zhang, Lynn VerPlank, Nathan Zahler, Salvatore DiBartolo, John F. Heneghan, Johannes S. Schloendorff, Jung Hee Suh, Paul Yan, Seth L. Alper, David J. Friedman, Martin R. Pollak
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Urology & Nephrology
Balajikarthick Subramanian, Hua Sun, Paul Yan, Victoria T. Charoonratana, Henry N. Higgs, Fang Wang, Ka-Man V. Lai, David M. Valenzuela, Elizabeth J. Brown, Johannes S. Schlondorff, Martin R. Pollak
KIDNEY INTERNATIONAL
(2016)
Editorial Material
Urology & Nephrology
Johannes Schlondorff
KIDNEY INTERNATIONAL
(2017)
Article
Biochemistry & Molecular Biology
Moran Dvela-Levitt, Maria Kost-Alimova, Maheswarareddy Emani, Eva Kohnert, Rebecca Thompson, Eriene-Heidi Sidhom, Ana Rivadeneira, Nareh Sahakian, Julie Roignot, Gregory Papagregoriou, Monica S. Montesinos, Abbe R. Clark, David McKinney, Juan Gutierrez, Mark Roth, Lucienne Ronco, Esther Elonga, Todd A. Carter, Andreas Gnirke, Michelle Melanson, Kate Hartland, Nicolas Wieder, Jane C-H Hsu, Constantinos Deltas, Rebecca Hughey, Anthony J. Bleyer, Stanislav Kmoch, Martina Zivna, Veronika Baresova, Savithri Kota, Johannes Schlondorff, Myriam Heiman, Seth L. Alper, Florence Wagner, Astrid Weinsz, Todd R. Golub, Eric S. Landeri, Anna Greka
Article
Biochemistry & Molecular Biology
Brianna E. Talbot, David H. Vandorpe, Brian R. Stotter, Seth L. Alper, Johannes S. Schlondorff
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Urology & Nephrology
Balajikarthick Subramanian, Justin Chun, Chandra Perez-Gill, Paul Yan, Isaac E. Stillman, Henry N. Higgs, Seth L. Alper, Johannes S. Schlondorff, Martin R. Pollak
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2020)
Article
Urology & Nephrology
Di Feng, Mukesh Kumar, Jan Muntel, Susan B. Gurley, Gabriel Birrane, Isaac E. Stillman, Lai Ding, Minxian Wang, Saima Ahmed, Johannes Schlondorff, Seth L. Alper, Tom Ferrante, Susan L. Marquez, Carlos F. Ng, Richard Novak, Donald E. Ingber, Hanno Steen, Martin R. Pollak
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2020)
Article
Urology & Nephrology
Amar Pandit, Johannes Schlondorff
KIDNEY INTERNATIONAL REPORTS
(2020)
Article
Urology & Nephrology
Hua Sun, Chandra Perez-Gill, Johannes S. Schlondorff, Balajikarthick Subramanian, Martin R. Pollak
Summary: Mutations in INF2 lead to mislocalized nephrin and podocytopathy, potentially due to its role in disrupting dynein-mediated trafficking of nephrin to lysosomes instead of recycling endosomes. Understanding this mechanism may facilitate the development of targeted therapies for INF2-mediated FSGS.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2021)
Editorial Material
Urology & Nephrology
Johannes S. Schlondorff
Summary: Genetics plays a significant role in kidney diseases, particularly in genes involved in maintaining and regulating the actin cytoskeleton of podocytes. A new study identified MYO9A as a gene linked to enhanced RhoA activity through human and mouse genetics. Unraveling the complex network of actin regulators remains a key challenge in understanding podocytopathies.
KIDNEY INTERNATIONAL
(2021)
Article
Multidisciplinary Sciences
Kimber L. Boekell, Brittney J. Brown, Brianna E. Talbot, Johannes S. Schlondorff
Summary: This study found that Trpc6 mutant can enhance platelet activation, including phosphatidylserine (PS) exposure, while confirming that TRPC6 is not necessary for this process. Furthermore, Trpc6 GOF disease mutants can affect pathways not usually modulated by TRPC6 channel activity, displaying a true gain-of-function phenotype.
Review
Surgery
Christie P. Thomas, Reem Daloul, Krista L. Lentine, Reginald Gohh, Prince M. Anand, Hila Milo Rasouly, Asif A. Sharfuddin, Johannes S. Schlondorff, Nancy M. Rodig, Margaret E. Freese, Neetika Garg, Brian K. Lee, Yasar Caliskan
Summary: The accessibility and cost reduction of genetic sequencing techniques have led to an expanded utilization of genetic testing in clinical practice. In the context of living kidney donation, genetic evaluation is increasingly used to identify genetic kidney disease in potential candidates, particularly younger individuals. However, there are numerous challenges and uncertainties associated with genetic testing for asymptomatic living kidney donors. Not all transplant practitioners are aware of the limitations of genetic testing or have access to specialized genetic counseling or expertise in renal genetics.
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Article
Multidisciplinary Sciences
Brittney J. Brown, Kimber L. Boekell, Brian R. Stotter, Brianna E. Talbot, Johannes S. Schlondorff
Summary: Mutations in TRPC6 gene are responsible for autosomal dominant focal segmental glomerulosclerosis in humans. A gain-of-function TRPC6 mutation in mice only confers mild susceptibility to glomerular injury.
Article
Physiology
Brian R. Stotter, Brianna E. Talbot, Diane E. Capen, Nadine Artelt, Junwei Zeng, Yasuyuki Matsumoto, Nicole Endlich, Richard D. Cummings, Johannes S. Schlondorff
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2020)
Review
Urology & Nephrology
Ali Poyan Mehr, Maryam Sadeghi-Najafabadi, Kristi Chau, Joseph Messmer, Rima Pai Neil Roy, David Friedman, Martin R. Pollak, Johannes Schlondorff, Mihran Naljayan, Tripti Singh, Stewart H. Lecker, Roger Rodby, Michael Germain, Helmut Rennke, Isaac E. Stillman
KIDNEY INTERNATIONAL REPORTS
(2019)