Article
Statistics & Probability
Yanyan Zeng, Daolin Pang, Hongyu Zhao, Tao Wang
Summary: In this study, a new method that takes into account the compositional nature of microbiome data is proposed to reduce the impact of zero values and estimate microbial compositions effectively. Simulations and an application to real data support the superior performance of the proposed method compared to existing methods.
JOURNAL OF THE AMERICAN STATISTICAL ASSOCIATION
(2022)
Article
Microbiology
Lu Yang, Jun Chen
Summary: This study provides a comprehensive evaluation of existing differential abundance analysis (DAA) tools, revealing that none of the evaluated methods are simultaneously robust, powerful, and flexible. To overcome the challenge of selecting the best DAA tool, the researchers designed an optimized procedure called ZicoSeq, which combines the strengths of existing methods and can be applied to diverse microbiome datasets for robust biomarker discovery.
Article
Statistics & Probability
Hee Cheol Chung, Irina Gaynanova, Yang Ni
Summary: Microorganisms play critical roles in host health. We propose a novel Bayesian graphical model that incorporates evolutionary history and zero inflation to quantify microbial counts using the latest microbiome profiling technique. Through simulation studies and analysis of real data, we find that this model offers higher accuracy in estimating microbial interaction networks.
ANNALS OF APPLIED STATISTICS
(2022)
Article
Environmental Sciences
Huifang Deng, Kitty E. Exel, Arno Swart, Axel A. Bonacic Marinovic, Cecile Dam-Deisz, Johanna W. B. van der Giessen, Marieke Opsteegh
Summary: Soil has been identified as an important source of exposure to Toxoplasma gondii, and a quantitative microbial risk assessment (QMRA) model was developed to estimate the risk associated with T. gondii exposure via accidental soil ingestion in the Netherlands. Detection of T. gondii oocysts and DNA in soil samples was successful, with an estimated incidence rate of infection ranging from 0.3 to 1.8 per 1000 individuals per day. Data gaps were identified, and the model structure can be used for more accurate risk estimates with additional data.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Microbiology
Gyung Jin Bahk, Hyo Jung Lee
Summary: In food microbiology, handling of censored data is limited and the practical application of maximum likelihood estimation (MLE) methods is restricted. Therefore, the development of a user-friendly Microbial-MLE tool for adjusting and processing observed counts for censored data in food microbiology is important.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Engineering, Environmental
Ernest R. Blatchley III, Haiying Cui
Summary: The study presents quantitative microbial risk assessment (QMRA) as a tool to evaluate the effectiveness of ultraviolet germicidal irradiation (UVGI) systems for indoor air disinfection. The model developed focuses on the implementation of UVGI systems for controlling SARS-CoV-2 infection. The findings suggest that UVGI systems can effectively control the risk of infection associated with SARS-CoV-2, with several factors influencing the transmission risk, including pathogen emission rate, UVGI fixture characteristics, and characteristics of the HVAC system. The QMRA framework provides a quantitative link between UVGI/HVAC system characteristics and changes in disease transmission risk. It is a useful tool for the design, testing, and validation of UVGI systems.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Mathematics
Zdenek Kala
Summary: This article explores the role of model uncertainties in sensitivity and probability analysis of reliability, proposing a new methodology called variance = entropy based on the equality of variance and entropy for quantifying model uncertainties in stochastic computational models without additional information. The paper demonstrates that Shannon entropy is a good sensitivity measure useful for quantifying model uncertainties.
Article
Ecology
Zachary Amir, Adia Sovie, Matthew Scott Luskin
Summary: Predator-prey dynamics are fundamental to ecology, but studying interactions directly is difficult. We developed a Bayesian hierarchical model that can infer predator-prey interactions and account for differences and uncertainties. Using this model, we analyzed camera trapping datasets from tropical forest landscapes in Southeast Asia and estimated four predator-prey relationships.
ECOLOGY AND EVOLUTION
(2022)
Article
Environmental Sciences
Zi-cheng Gui, Xiang Li, Man-li Liu, Zhang-di Peng, Cheng Yan, Zaheer Ahmad Nasir, Sonia Garcia Alcega, Frederic Coulon
Summary: This study assessed the seasonal variation of health risks associated with bioaerosol emission from a wastewater treatment plant. The results showed that airborne E. coli, fecal coliform, and Enterococcus concentrations were higher in the rotating-disc aeration mode compared to the microporous aeration mode. Field engineers had higher health risks than academic visitors, and the health risks of airborne Enterococcus were highest in summer. Wearing N95 masks could significantly reduce health risks.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Environmental Sciences
Cheng Yan, Zi-cheng Gui, Jun-ting Wu
Summary: The health risks of Gram-negative bacteria bioaerosol (GNBB) and Staphylococcus aureus bioaerosol (SAB) were assessed among academic visitors and staffs in a wastewater treatment plant. Results showed that males had higher health risks than females, and staffs had higher health risks than academic visitors.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Green & Sustainable Science & Technology
Anis Askari, Farzaneh Vahabzadeh, Mohammad Mahdi Mardanpour
Summary: A microbial fuel cell (MFC) based biosensor was developed to quantitatively determine LAS concentration in wastewater, achieving a linear correlation between current density and LAS concentration within a certain range. The MFC also demonstrated potential for power generation and purification in addition to its accurate biosensing capabilities.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Engineering, Environmental
Efaq Noman, Adel Al-Gheethi, Radin Maya Saphira Radin Mohamed, Balkis Talip, Mohamed Al-Sahari, Muhanna Al-Shaibani
Summary: The review discussed the microbiological risk assessment of Vibrio parahaemolyticus in prawn farm wastewaters and the use of nanoparticles to eliminate antibiotic-resistant bacteria. It emphasized the potential health effects of antibiotics in the environment and the role of aquaculture environments in contributing to antibiotic resistance gene transmission.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Mathematical & Computational Biology
Albert Kuo, Kasper D. Hansen, Stephanie C. Hicks
Summary: In complex tissues, single-nucleus RNA-sequencing (snRNA-seq) is preferred over single-cell RNA-sequencing (scRNA-seq) to measure gene expression. We show that droplet-based snRNA-seq data follow a negative binomial distribution and that quantification choices in adapting scRNA-seq to snRNA-seq can impact downstream analyses. Additionally, we confirm the presence of a gene length bias in snRNA-seq data and explore potential causes for the bias.
Article
Construction & Building Technology
Wajid Ali, Dong-zi An, Ya-fei Yang, Bei-bei Cui, Jia-xin Ma, Hao Zhu, Ming Li, Xiao-Jun Ai, Cheng Yan
Summary: This research compared the characteristics of Staphylococcus aureus bioaerosol emissions from squat and bidet toilets under different exposure heights for defecation and hand washing postures. The study found that the highest bioaerosol concentration was in squat toilets, and adult males faced a higher infection risk compared to other populations. Additionally, the infection risks were lower in hand washing postures compared to defecation postures.
BUILDING AND ENVIRONMENT
(2022)
Article
Microbiology
Tor Einar Moller, Sven Le Moine Bauer, Bjarte Hannisdal, Rui Zhao, Tamara Baumberger, Desiree L. Roerdink, Amandine Dupuis, Ingunn H. Thorseth, Rolf Birger Pedersen, Steffen Leth Jorgensen
Summary: This study investigates the microbial response to changes in oxygen concentration in deep-sea sediments. It finds that some abundant microbial taxa can be used to classify samples based on oxygen concentration. However, at the level of individual operational taxonomic units (OTUs), representatives of common taxa do not show differential abundance between high-oxic and low-oxic conditions. The results suggest that the abundance and prevalence of highly abundant OTUs may be better explained by variables other than oxygen.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Kostas Koutsoumanis, Ana Allende, Avelino Alvarez-Ordonez, Declan Bolton, Sara Bover-Cid, Marianne Chemaly, Robert Davies, Alessandra De Cesare, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Luisa Peixe, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Pier Sandro Cocconcelli, Pablo Salvador Fernandez Escamez, Miguel Prieto Maradona, Amparo Querol, Lolke Sijtsma, Juan Evaristo Suarez, Ingvar Sundh, Just Vlak, Fulvio Barizzone, Michaela Hempen, Lieve Herman
Summary: The qualified presumption of safety (QPS) approach is a generic pre-evaluation method for the safety of biological agents. It assesses published data for each agent based on taxonomic identity, relevant knowledge, and safety concerns. The QPS list has been updated to reflect the revised taxonomy, synonyms, and specific safety concerns.
Article
Environmental Sciences
Alessandro Foddai, Maarten Nauta, Johanne Ellis-Iversen
Summary: Simulating risk-based control of Campylobacter spp. in Danish broiler farms and flocks can reduce the potential risk of campylobacteriosis in consumers of poultry meat. Targeting high-risk farms/flocks for control measures could improve overall health outcomes.
MICROBIAL RISK ANALYSIS
(2022)
Article
Environmental Sciences
Maarten Nauta, Declan Bolton, Matteo Crotta, Johanne Ellis-Iversen, Thomas Alter, Michaela Hempen, Winy Messens, Marianne Chemaly
Summary: Quantitative microbiological risk assessment studies have shown that control options to reduce the concentration of Campylobacter spp. in broiler chicken caeca are effective at reducing the risk of human campylobacteriosis. However, recent studies suggest that the effectiveness of these control options may be less pronounced than previously believed, mainly due to lower values for the slope of the regression line.
MICROBIAL RISK ANALYSIS
(2022)
Article
Food Science & Technology
Konstantinos Koutsoumanis, Avelino Alvarez-Ordonez, Declan Bolton, Sara Bover-Cid, Marianne Chemaly, Robert Davies, Alessandra De Cesare, Lieve Herman, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Luisa Peixe, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Laurence Castle, Matteo Crotta, Konrad Grob, Maria Rosaria Milana, Annette Petersen, Artur Xavier Roig Sagues, Filipa Vinagre Silva, Eric Barthelemy, Anna Christodoulidou, Winy Messens, Ana Allende
Summary: High-pressure processing (HPP) is a non-thermal treatment method that uses isostatic pressures to inactivate microorganisms in food. The efficacy of HPP is influenced by various factors, including intrinsic properties of the food, external pressure and time applied, as well as the type and state of microorganisms. HPP does not pose additional safety concerns compared to other treatments like pasteurization. The effectiveness of HPP in reducing pathogens in milk is lower than the legal requirements for thermal pasteurization. Minimum HPP requirements can be identified to achieve specific reductions in pathogens in certain ready-to-eat cooked meat products.
Article
Food Science & Technology
Matthias Filter, Maarten Nauta, Sara M. Pires, Laurent Guillier, Tasja Buschhardt
Summary: Food microbiology researchers, risk assessment agencies, and food business operators face challenges in reusing data, models, and tools. The RAKIP Initiative has developed the FSKX knowledge exchange format and accompanying open-source software to facilitate knowledge sharing and application.
CURRENT OPINION IN FOOD SCIENCE
(2022)
Article
Food Science & Technology
Kostas Koutsoumanis, Ana Allende, Avelino Alvarez-Ordonez, Declan Bolton, Sara Bover-Cid, Marianne Chemaly, Robert Davies, Alessandra De Cesare, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Luisa Peixe, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Pier Sandro Cocconcelli, Pablo Salvador Fernandez Escamez, Miguel Prieto Maradona, Amparo Querol, Lolke Sijtsma, Juan Evaristo Suarez, Ingvar Sundh, Just Vlak, Fulvio Barizzone, Michaela Hempen, Sandra Correia, Lieve Herman
Summary: The qualified presumption of safety (QPS) approach provides a generic pre-evaluation of the safety of microorganisms in the food or feed chains. This assessment is regularly updated and supports the work of EFSA's Scientific Panels. The evaluation identified various microorganisms, but none of them were recommended for QPS status due to safety concerns or limited knowledge.
Article
Public, Environmental & Occupational Health
Maarten Nauta, Oliver McManus, Kristina Traeholt Franck, Ellinor Lindberg Marving, Lasse Dam Rasmussen, Stine Raith Richter, Steen Ethelberg
Summary: Wastewater surveillance and quantitative analysis of SARS-CoV-2 RNA are being used to monitor COVID-19 spread. A simulation model showed that there is a large variation in viral concentrations in wastewater samples, making it challenging to estimate incidence. Early detection of outbreaks using wastewater data is difficult, especially in small sampling areas and low-incidence situations. However, with a high number of shedders and consecutive testing, wastewater sampling performance is expected to improve significantly.
EPIDEMIOLOGY AND INFECTION
(2023)
Article
Public, Environmental & Occupational Health
Tariq Hantash, Ofosuhene O. O. Apenteng, Maarten Nauta, Hakan Vigre
Summary: A QMRA model was developed to evaluate the effectiveness of border control for Salmonella in frozen poultry products. The model consists of three modules and the current border control based on one sample per batch only achieves a 27% risk reduction.
Article
Food Science & Technology
Konstantinos Koutsoumanis, Ana Allende, Avelino Alvarez-Ordonez, Declan Bolton, Sara Bover-Cid, Marianne Chemaly, Alessandra De Cesare, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Luisa Peixe, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Pier Sandro Cocconcelli, Pablo Salvador Fernandez Escamez, Miguel Prieto Maradona, Amparo Querol, Lolke Sijtsma, Juan Evaristo Suarez, Ingvar Sundh, Just Vlak, Fulvio Barizzone, Sandra Correia, Lieve Herman
Summary: The qualified presumption of safety (QPS) approach is a regularly updated generic pre-evaluation of the safety of microorganisms in the food or feed chains. It is used to support the work of EFSA's Scientific Panels. In this study, 52 microorganisms were evaluated between April and September 2022.
Article
Food Science & Technology
Konstantinos Koutsoumanis, Ana Allende, Avelino Alvarez-Ordonez, Sara Bover-Cid, Marianne Chemaly, Alessandra De Cesare, Lieve Herman, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Luisa Peixe, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Bojan Blagojevic, Inge Van Damme, Michaela Hempen, Winy Messens, Declan Bolton
Summary: This study examines the impact of dry-ageing of beef and wet-ageing of beef, pork, and lamb on microbiological hazards and spoilage bacteria, and describes current practices. It differentiates between 'standard fresh' and wet-aged meat based on duration. The study collates data on key parameters (time, temperature, pH, and a(w)) using a literature survey and questionnaires. Various microbiological hazards and spoilage bacteria are identified in aged meats, and methods to prevent their growth are discussed. The study concludes that ageing meat under defined and controlled conditions can achieve similar or lower levels of microbiological hazards and spoilage bacteria than standard fresh meat preparation.
Article
Environmental Sciences
Alessandro Foddai, Maarten Nauta, Johanne Ellis-Iversen
Summary: The risk of human campylobacteriosis due to cross-contamination of Danish broiler flocks at slaughter was assessed. It was found that approximately 16% of the public health risk was attributable to cross-contaminated flocks. Reducing cross-contamination could significantly mitigate the risk of human campylobacteriosis.
MICROBIAL RISK ANALYSIS
(2023)
Article
Infectious Diseases
Ana Sofia R. Duarte, Ana Rita Marques, Vibe D. Andersen, Helle B. Korsgaard, Hanne Mordhorst, Frederik D. Moller, Thomas N. Petersen, Tine Hald, Frank M. Aarestrup
Summary: This study aimed to evaluate the relevance of indicator bacteria (Escherichia coli and Enterococcus faecalis) for AMR surveillance in pigs, and the utility of metagenomics. The results showed that metagenomics is a promising approach for AMR surveillance, as it allows for ranking of resistance genes and correlation analysis with antimicrobial use.
Article
Infectious Diseases
Sara Perestrelo, Ana Amaro, Michael S. M. Brouwer, Lurdes Clemente, Ana Sofia Ribeiro Duarte, Annemarie Kaesbohrer, Renata Karpiskova, Vicente Lopez-Chavarrias, Dearbhaile Morris, Deirdre Prendergast, Angela Pista, Leonor Silveira, Magdalena Skarzynska, Rosemarie Slowey, Kees T. Veldman, Magdalena Zajac, Catherine Burgess, Julio Alvarez
Summary: Antimicrobial resistance (AMR) is a significant threat to public health, and Escherichia coli resistant to extended spectrum cephalosporins (ESC-EC) exemplifies the One Health problem due to its global distribution. The complex epidemiology of ESC-EC infection involves multiple sources, requiring a comprehensive database comprising information from animal, human, and environmental sources. A database consisting of phenotypic and genetic information on 10,763 ESC-EC isolates from multiple sources and countries was assembled, providing a foundation for assessing geographical and temporal trends and transmission dynamics in animals and humans.
Article
Food Science & Technology
Konstantinos Koutsoumanis, Ana Allende, Avelino Alvarez-Ordonez, Declan Bolton, Sara Bover-Cid, Marianne Chemaly, Alessandra de Cesare, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Luisa Peixe, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Pier Sandro Cocconcelli, Pablo Salvador Fernandez Escamez, Miguel Prieto Maradona, Amparo Querol, Lolke Sijtsma, Juan Evaristo Suarez, Ingvar Sundh, Just Vlak, Fulvio Barizzone, Michaela Hempen, Sandra Correia, Lieve Herman
Summary: The qualified presumption of safety (QPS) is a generic pre-assessment method that supports the work of EFSA's Scientific Panels by evaluating the safety of microorganisms in the food or feed chains. The evaluation is based on the body of knowledge and safety concerns of the microorganisms. Some strains, such as filamentous fungi, bacteriophages, oomycetes, and certain bacteria, are excluded from the QPS assessment due to their potentially harmful traits. The QPS list is regularly reviewed and updated, and new TUs can receive QPS status if they meet the necessary qualifications.
Article
Public, Environmental & Occupational Health
Pedro Moura, Birgitte Borck Hog, Lis Alban, Ute Wolff Sonksen, Ana Sofia Ribeiro Duarte, Marianne Sandberg
Summary: This study evaluated the efficacy of the Danish Integrated Antimicrobial Resistance Monitoring and Research Program (DANMAP) using OH-EpiCap, a comprehensive evaluation tool. It found that DANMAP has a strong One Health approach for animals and humans, but lacks consideration for environmental aspects.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Food Science & Technology
Guangfei Wei, Yichuan Liang, Guozhuang Zhang, Zhaoyu Zhang, Yongqing Zhang, Shilin Chen, Linlin Dong
Summary: This study investigated the presence of fungi on the surface of Polygonum multiflorum Thunb and found that it is easily contaminated by toxigenic fungi and mycotoxins, posing a health risk for consumers. The results also showed that sampling location and processing can influence the assembly and network complexity of the surface microbiome of Polygonum multiflorum Thunb.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Gabriele Busetta, Giuliana Garofalo, Salvatore Claps, Maria Teresa Sardina, Elena Franciosi, Antonio Alfonzo, Nicola Francesca, Giancarlo Moschetti, Luca Settanni, Raimondo Gaglio
Summary: This study evaluates the microbial diversity of wooden shelves used for the ripening of PDO Pecorino di Filiano and PGI Canestrato di Moliterno cheeses. The results show a strict correlation between the microbiota of wooden shelves and cheese rinds, indicating the positive role of wooden shelves in the cheese ripening process.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Massimo Todaro, Giuliana Garofalo, Gabriele Busetta, Riccardo Gannuscio, Ambra Rita Di Rosa, Maria Luisa Scatassa, Cinzia Cardamone, Isabella Mancuso, Elena Franciosi, Fatima Rando, Monica Agnolucci, Vincenzo Chiofalo, Raimondo Gaglio, Luca Settanni
Summary: This study aimed to reduce the production time of PDO Pecorino Siciliano cheese by comparing two cooking methods and assessing their impact on microbiological composition and lactic acid bacteria populations. The results showed that the different cooking methods did not affect the physicochemical characteristics of the cheese, and sensory evaluation confirmed that the modified production protocol did not significantly alter the product characteristics and overall acceptance. Therefore, cooking under hot water proved to be an effective way to reduce the transformation duration while maintaining the typicality of PDO Pecorino Siciliano cheese.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Yingying Sun, Yana Liu, Weiwei Zhou, Lele Shao, Han Wang, Yijie Zhao, Bo Zou, Xingmin Li, Ruitong Dai
Summary: The study evaluates the effect of Ohmic heating (OH) at various voltage gradients and water bath on microbial inactivation, physicochemical and sensory properties, and microbial flora of pasteurized milk. Results show that OH with higher voltage effectively inactivates microorganisms, slows down lipid oxidation, and maintains sensory quality and amino acids content of milk. The microbial community in milk treated with OH remains relatively stable during storage.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Minjin Oh, Ana Cevallos-Urena, Byoung Sik Kim
Summary: In this study, two bacteriophages, PECP14 and PECP20, infecting Escherichia coli O157:H7 were isolated and found to be stable under different environmental conditions. These phages exhibited specificity in targeting different host receptors and demonstrated the ability to infect other foodborne pathogens. The efficacy of phage treatment was demonstrated through rapid adsorption and bacterial lysis. Furthermore, purified endolysins derived from these phages showed remarkable bacteriolytic activity not only against E. coli O157:H7 but also against other pathogens. These findings suggest that these phages and their endolysins can be used for biological control of E. coli O157:H7 in various stages of agricultural product production.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Review
Food Science & Technology
Filippo Marzoli, Michela Bertola, Juliane Pinarelli Fazion, Giulia Cento, Pietro Antonelli, Beatrice Dolzan, Lisa Barco, Simone Belluco
Summary: Insects are a sustainable and protein-rich food source, and Salmonella is a common pathogen in insects. This systematic review aims to collect and evaluate existing studies on the occurrence of Salmonella in crickets and mealworms. Although the number of studies is limited and there are some limitations, this review highlights the importance of obtaining reliable data on the presence of Salmonella in insects, considering the growing market and increased farming scale.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Review
Food Science & Technology
Lucas Chikhi, Melanie Mancier, Hubert Brugere, Bertrand Lombard, Lyazrhi Faouzi, Laurent Guillier, Nathalie Gnanou Besse
Summary: Listeriosis is a severe foodborne disease with a high mortality rate, mainly caused by L. monocytogenes. The EU regulation sets qualitative or quantitative food safety criteria for L. monocytogenes and recommends the use of EN ISO 11290-1 as the reference detection method. Our study compares alternative certified methods for detecting L. monocytogenes, highlighting the importance of diversifying available detection methods.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Christina A. M. DeWitt, Kevin A. Nelson, Hyung Joo Kim, David H. Kingsley
Summary: High pressure processing (HPP) is a non-thermal method that can effectively inactivate pathogens. Research has shown that applying high pressure to thawed samples in an ultra-low temperature environment enhances the inactivation of norovirus.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Suzane Manzini, Thaina Valente Bertozzo, Isabella Neves Aires, Nassarah Jabur Lot Rodrigues, Amanda Bezerra Bertolini, Marcela Alexandrino, Jackieline Sampaio Steinle, Renata Pimentel Bandeira de Melo, Rinaldo Aparecido Mota, Maria Izabel Merino de Medeiros, Virginia Bodelao Richini-Pereira, Vera Claudia Lorenzetti Magalhaes Curci, Simone Baldini Lucheis
Summary: This study detected Toxoplasma gondii DNA in raw bovine milk samples from expansion tanks in small properties located in different cities of the Midwest region of Sa similar to o Paulo, Brazil using PCR. The results showed that the TgNP1 and TgNP2 genes were more efficient in detecting T. gondii DNA, highlighting the importance of raw bovine milk as a potential source of human infections caused by this parasite, and emphasizing the need for proper pasteurization and further understanding of the epidemiology of this protozoan.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Luca Bolzoni, Mauro Conter, Luca Lamperti, Erika Scaltriti, Marina Morganti, Antonio Poeta, Marco Vecchi, Silvia Paglioli, Alessandra Rampini, Paolo Ramoni, Daniela De Vita, Cristina Bacci, Martina Rega, Laura Andriani, Stefano Pongolini, Silvia Bonardi
Summary: This study assessed the role of equids as carriers of Salmonella and the occurrence of contaminated equid carcasses during the slaughter process. Salmonella was detected in a small percentage of equids' caecal contents, but not in carcass samples. Additionally, a genetic match was found between Salmonella strains from horse caecal contents and human cases, suggesting a link between horse meat consumption and salmonellosis.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Daria Nowinski, Tomasz Czapka, Irena Maliszewska
Summary: The effect of multiple sublethal doses of non-thermal plasma treatments on phenotypical changes and the reduction in phytopathogenicity of three species of fungi was investigated. The plasma damaged the surface of the fungal hyphae, decreased their dry biomass, and affected the activity of pectinase and xylanase. Furthermore, the plasma treatments increased the germination rate of artificially infected cucumber seeds and resulted in plants with higher biomass and longer roots and stems. However, the reduction in phytopathogenicity was not complete after the multiple plasma treatments.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)