Article
Food Science & Technology
Andrew M. Schwartz, Haley A. Marcotte, Crystal N. Johnson
Summary: Through experimentation, we found that the Cell Mosaic in-house conjugation method is a viable alternative to enumerate vibrios in oysters, and milk can be used as a blocking reagent instead of alkaline phosphatase-conjugated probes. These modifications are crucial for scientists to accurately quantify pathogens in food products.
Article
Plant Sciences
Qingcong He, Dongwei Wang, Bei Tang, Jian Wang, Deyong Zhang, Yong Liu, Feixue Cheng
Summary: In this study, specific primers were designed for rapid identification and quantification of the plant-parasitic nematode Meloidogyne graminicola in soil. Both LAMP and real-time PCR showed higher sensitivity in detecting the nematode compared to conventional PCR. These methods have the potential to provide a useful platform for rapid species identification and quantification of economically important PPNs.
Article
Food Science & Technology
Regina Klapper, Ute Schroder
Summary: Seafood products are vulnerable to food fraud, highlighting the importance of reliable authentication methods. This study developed a multiplex TaqMan real-time PCR assay for rapid identification of commercially important scallop species.
Article
Multidisciplinary Sciences
Walid Hossain, Mohabbat Hossain, Khalid Arafath, Subarna Sayed Ety, Mahade Hasan Shetu, Mazbahul Kabir, Farjana Akther Noor, Kalissar Mannoor
Summary: The study aimed to reduce the run time of multiplex RT-PCR for detecting SARS CoV-2 N1 and N2 sequences and human RNase P (RP) sequence. By optimizing the Fast PCR protocol and comparing it with conventional PCR, the researchers found that Fast PCR significantly shortened the run time while yielding comparable results to conventional PCR.
Article
Food Science & Technology
Christian Brenn, Ute Schroeder, Reinhold Hanel, Pedro Martinez Arbizu
Summary: The study presents a TaqManprobe based multiplex real-time PCR screening assay for identification of four commercially relevant crustacean species, showing high specificity and potential for rapid species identification.
Article
Chemistry, Multidisciplinary
Bong Kyun Kim, Sang-A Lee, Minju Park, Eui Ju Jeon, Mi Jung Kim, Jung Min Kim, Heesuk Kim, Seungwon Jung, Sang Kyung Kim
Summary: This article introduces a photonic PCR platform using hydrogel microparticles, which utilizes photothermal nanomaterials as heating elements for rapid and portable POCT. The researchers successfully demonstrate the ability to perform multiplex assays by loading multiple encoded pPIN microparticles in a single reaction.
Article
Microbiology
Hanan R. Shehata, Basma Hassane, Steven G. Newmaster
Summary: This study developed real-time PCR methods for strain specific identification and enumeration of L. paracasei 8700:2, which proved to be strain specific, highly sensitive, and able to count viable but non culturable cells.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Intiaz Amin Chowdhury, Guiping Yan
Summary: A rapid and accurate PCR method was developed for detecting and identifying a new species of root-lesion nematode. Species-specific primers showed high specificity in both conventional and quantitative real-time PCR assays, with high detection sensitivity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Astronomy & Astrophysics
Johannes V. Roth, Dominik Schweitzer, Leon J. Sieke, Lorenz von Smekal
Summary: This study develops and compares different real-time methods for calculating spectral functions, including classical-statistical simulations, the Gaussian state approximation, and functional renormalization group methods. Results show that at high temperatures, the different methods yield consistent results, but at low temperatures, the real-time FRG method qualitatively approaches the exact solution.
Article
Pathology
B. S. W. Chong, K. J. Kennedy
Summary: This study compared the accuracy and turnaround time of the BioFire FilmArray Meningitis/Encephalitis multiplex PCR panel to traditional testing algorithms for the diagnosis of meningitis-encephalitis. The ME panel showed high concordance with traditional testing, streamlined the laboratory workflow, and significantly reduced turnaround time. However, the failure of the ME panel to detect Cryptococcus spp. suggests the need for additional testing methods in cases where cryptococcal meningitis is suspected. Despite molecular assays targeting common causes of CNS infection, the diagnostic yield remains suboptimal.
Article
Agriculture, Dairy & Animal Science
V. Vigolo, M. Franzoi, F. Cendron, G. Salvadore, M. Penasa, M. Cassandro, M. De Marchi
Summary: This study compared different methods and technical engagement for identifying the A1 and A2 variants of 0-casein in milk. The findings showed that automated DNA extraction and chromatoallele-specific PCR techniques are fast and reliable options.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Karanth Padyana Anupama, Ashwath Nayak, Iddya Karunasagar, Indrani Karunasagar, Biswajit Maiti
Summary: The study successfully developed a LAMP assay for detecting pathogenic Vibrio parahaemolyticus, which exhibited good specificity and high sensitivity, enabling direct detection of the pathogen in seafood samples.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Infectious Diseases
Evonne N. Woodson, Samantha S. Katz, Sheree S. Mosley, Damien C. Danavall, Katherine E. Bowden, Kai-Hua Chi, Brian H. Raphael
Summary: A rapid real-time PCR method for differentiating Chlamydia trachomatis infections associated with LGV or non-LGV serovars showed a sensitivity of 87.5% for LGV detection and high specificity, accuracy, and reproducibility. The performance characteristics were between 93% and 100% with a detection limit of <= 100 copies/reaction, comparable to the reference method of Sanger sequencing.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2021)
Article
Food Science & Technology
Abel Guillermo Rios-Castillo, Carolina Ripolles-Avila, Jose Juan Rodriguez-Jerez
Summary: This study evaluated the capacity of real-time PCR and conventional culture methods to detect Salmonella enterica serovar Typhimurium and Listeria monocytogenes adhered to stainless steel surfaces used as food contact surfaces. Real-time PCR showed a higher detection capacity for low concentration samples, and even detected samples without pre-enrichment. However, pre-enrichment is still recommended to avoid false positive and false negative results.
Article
Environmental Sciences
Kevin J. Lyons, Jenni Ikonen, Anna-Maria Hokajarvi, Teemu Rasanen, Tarja Pitkanen, Ari Kauppinen, Katharina Kujala, Pekka M. Rossi, Ilkka T. Miettinen
Summary: Groundwater quality monitoring often relies on sporadic and slow manual sampling and laboratory analyses, which may result in undetected water quality deficiencies. In Finland, enhanced monitoring of untreated groundwater quality using real-time online monitoring, isotopic analysis, and microbial community analysis revealed indications of surface water intrusion and faecal contamination. The findings demonstrated the usefulness of supplementary methods and suggested their wider implementation to enhance groundwater quality monitoring.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Editorial Material
Microbiology
Jens A. Hammerl, Jennifer M. Ritchie, Francesca Leoni, Swapan Banerjee, Eckhard Strauch
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Fisheries
Marion Soree, Anna Le Meleder, Elise Maurouard, Solen Lozach, Mathias Papin, Christophe Stavrakakis, Corinne Audemard, Dominique Hervio Heath, Lionel Degremont
Summary: In this study, we evaluated the impact of ploidy in Crassostrea gigas oysters on contamination with indigenous Vibrio parahaemolyticus (Vp) and on the accumulation and depuration of Vp in laboratory experiments. The results showed that ploidy did not significantly influence contamination by indigenous Vp, although triploid oysters had a lower contamination trend in November. The pathogenic profiles of indigenous Vp varied between seasons, and depuration was higher during the summer months.
Article
Microbiology
Elisa Taviani, Harold van den Berg, Fernando Nhassengo, Eugenia Nguluve, Jussa Paulo, Olivia Pedro, Giuliana Ferrero
Summary: The microbiological quality of drinking water in Moamba, Mozambique was assessed, revealing the presence of fecal contamination and bacterial pathogens. Additionally, these pathogens exhibited multidrug resistance, further increasing the health risks for the population.
Article
Immunology
Carla Palacios-Gorba, Alexandra Moura, Yuval Markovich, Nathalie Tessaud-Rita, Angel Gomez-Martin, Helene Bracq-Dieye, Jesus Gomis, Guillaume Vales, Maria Pastor-Martin, Pierre Thouvenot, Carles Escrig, Alexandre Leclercq, Marc Lecuit, Juan J. Quereda
Summary: Two species of pathogenic Listeria, L. monocytogenes and L. ivanovii, can asymptomatically colonize udders and tonsils of ruminants without fecal shedding. Their whole-genome sequences contain known core virulence and antibiotic resistance genes. This study highlights the importance of udders and tonsils as intra-host sites of Listeria colonization.
MICROBES AND INFECTION
(2023)
Review
Microbiology
Elisa Albini, Massimiliano Orso, Francesco Cozzolino, Luca Sacchini, Francesca Leoni, Chiara Francesca Magistrali
Summary: This study investigated the prevalence of antimicrobial resistance (AMR) in bacterial isolates from bivalves through a systematic review. The results showed the presence of antibiotic-resistant bacteria, including those resistant to critically important antimicrobials (HPCIA), in marine bivalves, posing a risk to consumers.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Serena Simoni, Francesca Leoni, Laura Veschetti, Giovanni Malerba, Maria Carelli, Maria M. Lleo, Andrea Brenciani, Gianluca Morroni, Eleonora Giovanetti, Elena Rocchegiani, Francesca Barchiesi, Carla Vignaroli
Summary: This study reports the recovery and characterization of a multidrug-resistant, KPC-3-producing Klebsiella michiganensis isolated from Venus clam samples. Whole-genome sequencing analysis revealed the presence of multiple plasmids carrying various beta-lactamase genes. Curing of the strain resulted in the loss of beta-lactamase genes and rearrangement of plasmids. This study highlights the importance of understanding the transmission of carbapenem resistance outside of the hospital setting and the potential role of bivalves in the spread of pathogens and resistance genes.
MICROBIOLOGY SPECTRUM
(2023)
Article
Immunology
Deyan Stratev, Rumyana Fasulkova, Gergana Krumova-Valcheva
Summary: The study aimed to investigate the occurrence, pathogenic factors, and antimicrobial resistance of Vibrio parahaemolyticus in seafood sold in Bulgarian retail shops. A total of 180 seafood samples including sea fish, mussels, oysters, veined rapa whelks, shrimps, and squids were analyzed. PCR methods were used to identify V. parahaemolyticus and detect tdh and trh genes. The results showed that 24% of the samples were positive for V. parahaemolyticus, with no tdh-positive strains found and only one veined rapa whelk isolate carrying the trh gene. Antimicrobial susceptibility testing revealed susceptibility to most antibiotics, with intermediate resistance observed for a few. The study highlights the presence of pathogenic and multidrug-resistant V. parahaemolyticus strains in seafood, posing a risk to consumer health.
MICROBIAL PATHOGENESIS
(2023)
Article
Microbiology
Gergana Lyubomirova Krumova-Valcheva, Ilaria Di Bartolo, Richard Piers Smith, Eva Gyurova, Gergana Mateva, Mihail Milanov, Albena Dimitrova, Elke Burow, Hristo Daskalov
Summary: This study aimed to evaluate the presence of HEV RNA in farrow-to-finish pig farms in different regions of Bulgaria. The results showed that out of 32 tested farms, HEV RNA was detected in 12 (37.5%) farms. The highest percentage of HEV-positive samples was found in pooled fecal samples from finisher pigs (20.6%), while the detection rate was lower in dry sows and gilts. The study confirms the circulation of HEV in pig farms in Bulgaria and indicates a potential risk to public health.
Article
Food Science & Technology
Monika Ziomek, Michal Gondek, Beatrice Torracca, Francesca Marotta, Giuliano Garofolo, Kinga Wieczorek, Katarzyna Michalak, Filippo Fratini, Francesca Pedonese
Summary: A study examined the presence of Campylobacter species in wild boars in Tuscany to determine their contribution to human infection through the food chain. The study found that 44.56% of the animals and various samples were contaminated with Campylobacter spp., with C. coli and C. lanienae being the dominant species. The study emphasizes the importance of providing food safety information to hunters and consumers due to the contamination of meat and liver with Campylobacter spp.
Article
Biotechnology & Applied Microbiology
Carolina Rosa Rodrigues de Souza, Helene Bergis, Ng Patricia, Laurent Guillier, Benjamin Felix, Alexandre Leclercq, Nathalie Gnanou Besse
Summary: Listeria monocytogenes can grow and contaminate various food categories under stressful conditions. Advances in DNA sequencing-based identification methods have enabled more accurate characterization of the pathogen. Understanding the growth potentials of L. monocytogenes is crucial for risk assessment and detection across genetic diversity of different clonal complexes (CCs).
Article
Biotechnology & Applied Microbiology
Evelyn Pereira, Amanda Conrad, Adiam Tesfai, Alexandra Palacios, Rima Kandar, Ashley Kearney, Annie Locas, Fred Jamieson, Elisa Elliot, Mark Otto, Kerry Kurdilla, Mary Tijerina, Insook Son, James B. Pettengill, Yi Chen, Teresa Fox, Chris Lane, Ryan Aguillon, Jasmine Huffman, Mabel Sheau Fong Low, Matthew Wise, Lauren Edwards, Sally Bidol, Heather M. Blankenship, Hilary E. Rosen, Alexandre Leclercq, Marc Lecuit, Mathieu Tourdjman, Hubert Herber, Lauren S. Singleton, Stelios Viazis, Michael C. Bazaco
Summary: International collaboration is crucial for ensuring the safety of the global food supply. A recent outbreak of Listeria monocytogenes infections linked to imported enoki mushrooms required coordination and investigation by multiple countries.
JOURNAL OF FOOD PROTECTION
(2023)
Article
Cell Biology
Caroline Charlier, Coralie Noel, Lukas Hafner, Alexandra Moura, Claire Mathiaud, Aurelia Pitsch, Chakib Meziane, Lydie Jolly-Sanchez, Astrid de Pontfarcy, Sylvain Diamantis, Helene Bracq-Dieye, Olivier Disson, Pierre Thouvenot, Guillaume Vales, Nathalie Tessaud-Rita, Mathieu Tourdjman, Alexandre Leclercq, Marc Lecuit
Summary: We present a case of fulminant neonatal listeriosis caused by horizontal transmission of Listeria monocytogenes (Lm) in a neonatal double room. Genomic analysis demonstrates a close genetic relationship between clinical isolates, supporting the idea of cross-contamination. Oral inoculation experiments in both adult and neonatal mice show that newborns are susceptible to a low Lm inoculum due to the immaturity of their gut microbiota. Therefore, infected neonates should be isolated until they stop shedding Lm in their feces to prevent horizontal transmission and its severe consequences.
CELL REPORTS MEDICINE
(2023)
Review
Veterinary Sciences
Jacek Osek, Kinga Wieczorek
Summary: Listeria monocytogenes is a dangerous food-borne pathogen that poses a severe threat to human health. It has the ability to survive and multiply in various challenging environmental conditions, making it difficult to eradicate and increasing the risk of food contamination.
JOURNAL OF VETERINARY RESEARCH
(2023)
Article
Veterinary Sciences
Miguel Garcia-de la Virgen, Inmaculada Lopez-Almela, Alexandra Moura, Sergio Vazquez, Sara Perez-Montagud, Alexandre Leclercq, Marc Lecuit, Juan J. Quereda
Summary: This report describes a case of Listeria monocytogenes-associated mesenteric lymphadenitis in a cat, highlighting the importance of this syndrome for veterinary clinicians and microbiologists in terms of health and food safety for animals and humans.
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2023)
Article
Veterinary Sciences
Beata Lachtara, Kinga Wieczorek, Jacek Osek
Summary: Antibiotic resistance has emerged among L. monocytogenes strains isolated from food and food-production environments in Poland, posing a potential health risk to consumers. Most of the tested strains displayed resistance to multiple antibacterial substances.
JOURNAL OF VETERINARY RESEARCH
(2023)
Article
Food Science & Technology
Guangfei Wei, Yichuan Liang, Guozhuang Zhang, Zhaoyu Zhang, Yongqing Zhang, Shilin Chen, Linlin Dong
Summary: This study investigated the presence of fungi on the surface of Polygonum multiflorum Thunb and found that it is easily contaminated by toxigenic fungi and mycotoxins, posing a health risk for consumers. The results also showed that sampling location and processing can influence the assembly and network complexity of the surface microbiome of Polygonum multiflorum Thunb.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Gabriele Busetta, Giuliana Garofalo, Salvatore Claps, Maria Teresa Sardina, Elena Franciosi, Antonio Alfonzo, Nicola Francesca, Giancarlo Moschetti, Luca Settanni, Raimondo Gaglio
Summary: This study evaluates the microbial diversity of wooden shelves used for the ripening of PDO Pecorino di Filiano and PGI Canestrato di Moliterno cheeses. The results show a strict correlation between the microbiota of wooden shelves and cheese rinds, indicating the positive role of wooden shelves in the cheese ripening process.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Massimo Todaro, Giuliana Garofalo, Gabriele Busetta, Riccardo Gannuscio, Ambra Rita Di Rosa, Maria Luisa Scatassa, Cinzia Cardamone, Isabella Mancuso, Elena Franciosi, Fatima Rando, Monica Agnolucci, Vincenzo Chiofalo, Raimondo Gaglio, Luca Settanni
Summary: This study aimed to reduce the production time of PDO Pecorino Siciliano cheese by comparing two cooking methods and assessing their impact on microbiological composition and lactic acid bacteria populations. The results showed that the different cooking methods did not affect the physicochemical characteristics of the cheese, and sensory evaluation confirmed that the modified production protocol did not significantly alter the product characteristics and overall acceptance. Therefore, cooking under hot water proved to be an effective way to reduce the transformation duration while maintaining the typicality of PDO Pecorino Siciliano cheese.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Yingying Sun, Yana Liu, Weiwei Zhou, Lele Shao, Han Wang, Yijie Zhao, Bo Zou, Xingmin Li, Ruitong Dai
Summary: The study evaluates the effect of Ohmic heating (OH) at various voltage gradients and water bath on microbial inactivation, physicochemical and sensory properties, and microbial flora of pasteurized milk. Results show that OH with higher voltage effectively inactivates microorganisms, slows down lipid oxidation, and maintains sensory quality and amino acids content of milk. The microbial community in milk treated with OH remains relatively stable during storage.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Minjin Oh, Ana Cevallos-Urena, Byoung Sik Kim
Summary: In this study, two bacteriophages, PECP14 and PECP20, infecting Escherichia coli O157:H7 were isolated and found to be stable under different environmental conditions. These phages exhibited specificity in targeting different host receptors and demonstrated the ability to infect other foodborne pathogens. The efficacy of phage treatment was demonstrated through rapid adsorption and bacterial lysis. Furthermore, purified endolysins derived from these phages showed remarkable bacteriolytic activity not only against E. coli O157:H7 but also against other pathogens. These findings suggest that these phages and their endolysins can be used for biological control of E. coli O157:H7 in various stages of agricultural product production.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Review
Food Science & Technology
Filippo Marzoli, Michela Bertola, Juliane Pinarelli Fazion, Giulia Cento, Pietro Antonelli, Beatrice Dolzan, Lisa Barco, Simone Belluco
Summary: Insects are a sustainable and protein-rich food source, and Salmonella is a common pathogen in insects. This systematic review aims to collect and evaluate existing studies on the occurrence of Salmonella in crickets and mealworms. Although the number of studies is limited and there are some limitations, this review highlights the importance of obtaining reliable data on the presence of Salmonella in insects, considering the growing market and increased farming scale.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Review
Food Science & Technology
Lucas Chikhi, Melanie Mancier, Hubert Brugere, Bertrand Lombard, Lyazrhi Faouzi, Laurent Guillier, Nathalie Gnanou Besse
Summary: Listeriosis is a severe foodborne disease with a high mortality rate, mainly caused by L. monocytogenes. The EU regulation sets qualitative or quantitative food safety criteria for L. monocytogenes and recommends the use of EN ISO 11290-1 as the reference detection method. Our study compares alternative certified methods for detecting L. monocytogenes, highlighting the importance of diversifying available detection methods.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Christina A. M. DeWitt, Kevin A. Nelson, Hyung Joo Kim, David H. Kingsley
Summary: High pressure processing (HPP) is a non-thermal method that can effectively inactivate pathogens. Research has shown that applying high pressure to thawed samples in an ultra-low temperature environment enhances the inactivation of norovirus.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Suzane Manzini, Thaina Valente Bertozzo, Isabella Neves Aires, Nassarah Jabur Lot Rodrigues, Amanda Bezerra Bertolini, Marcela Alexandrino, Jackieline Sampaio Steinle, Renata Pimentel Bandeira de Melo, Rinaldo Aparecido Mota, Maria Izabel Merino de Medeiros, Virginia Bodelao Richini-Pereira, Vera Claudia Lorenzetti Magalhaes Curci, Simone Baldini Lucheis
Summary: This study detected Toxoplasma gondii DNA in raw bovine milk samples from expansion tanks in small properties located in different cities of the Midwest region of Sa similar to o Paulo, Brazil using PCR. The results showed that the TgNP1 and TgNP2 genes were more efficient in detecting T. gondii DNA, highlighting the importance of raw bovine milk as a potential source of human infections caused by this parasite, and emphasizing the need for proper pasteurization and further understanding of the epidemiology of this protozoan.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Luca Bolzoni, Mauro Conter, Luca Lamperti, Erika Scaltriti, Marina Morganti, Antonio Poeta, Marco Vecchi, Silvia Paglioli, Alessandra Rampini, Paolo Ramoni, Daniela De Vita, Cristina Bacci, Martina Rega, Laura Andriani, Stefano Pongolini, Silvia Bonardi
Summary: This study assessed the role of equids as carriers of Salmonella and the occurrence of contaminated equid carcasses during the slaughter process. Salmonella was detected in a small percentage of equids' caecal contents, but not in carcass samples. Additionally, a genetic match was found between Salmonella strains from horse caecal contents and human cases, suggesting a link between horse meat consumption and salmonellosis.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Daria Nowinski, Tomasz Czapka, Irena Maliszewska
Summary: The effect of multiple sublethal doses of non-thermal plasma treatments on phenotypical changes and the reduction in phytopathogenicity of three species of fungi was investigated. The plasma damaged the surface of the fungal hyphae, decreased their dry biomass, and affected the activity of pectinase and xylanase. Furthermore, the plasma treatments increased the germination rate of artificially infected cucumber seeds and resulted in plants with higher biomass and longer roots and stems. However, the reduction in phytopathogenicity was not complete after the multiple plasma treatments.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)