Article
Biochemistry & Molecular Biology
Santosh Phuyal, Elena Djaerff, Anabel-Lise Le Roux, Martin J. Baker, Daniela Fankhauser, Sayyed Jalil Mahdizadeh, Veronika Reiterer, Amirabbas Parizadeh, Edward Felder, Jennifer C. Kahlhofer, David Teis, Marcelo G. Kazanietz, Stephan Geley, Leif Eriksson, Pere Roca-Cusachs, Hesso Farhan
Summary: Mechanical strain induces the formation of ER exit sites and accelerates ER-to-Golgi trafficking. Impaired ER exit sites function results in reduced ability of cells to expand their surface area under mechanical stress and increases susceptibility to plasma membrane defects. Rac1 plays a crucial role in linking mechanical strain to ER export.
Article
Multidisciplinary Sciences
Yong Bhum Song, Seung-Yeol Park, Kunyou Park, Hayoung Hwang, Rona S. Carroll, Victor W. Hsu, Ursula B. Kaiser
Summary: G protein-coupled receptors (GPCRs) play crucial roles in physiological and pathological processes, and mutations can lead to diseases. This study focuses on PROKR2 and its mutant causing hypogonadotropic hypogonadism, and identifies distinct interactomes and post-ER itinerary that determine the fate of the wild-type and mutant receptors. The study also reveals the role of the post-ER itinerary in reducing ER stress induced by mutant PROKR2.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Ke Yang, Min Liu, Zhi Feng, Marta Rojas, Lingjian Zhou, Hongmei Ke, Jose Carlos Pastor-Pareja
Summary: Secretory cargos are collected at ERES before transport to Golgi. In Drosophila models, a pre-cis-Golgi region equivalent to the ERGIC in vertebrates is involved in both anterograde and retrograde transport, connecting ERES and the rest of the Golgi without evidence of separate compartments or large carriers. Many vesicles and pearled tubules are observed in this process.
Article
Biochemistry & Molecular Biology
Aubrey Weigel, Chi-Lun Chang, Gleb Shtengel, C. Shan Xu, David P. Hoffman, Melanie Freeman, Nirmala Iyer, Jesse Aaron, Satya Khuon, John Bogovic, Wei Qiu, Harald F. Hess, Jennifer Lippincott-Schwartz
Summary: Cellular versatility relies on accurate trafficking of proteins to their organelles, particularly through the secretory pathway which involves an elaborate network of interconnected tubules originating from the endoplasmic reticulum. COPII protein regulates cargo entry at the ER neck region, while COPI protein escorts tubular entities towards the Golgi apparatus via microtubule-directed movement.
Article
Biochemistry & Molecular Biology
Patricia Franzka, Svenja Caren Schueler, Takfarinas Kentache, Robert Storm, Andrea Bock, Istvan Katona, Joachim Weis, Katrin Buder, Christoph Kaether, Christian A. Hubner
Summary: Proteins of the secretory pathway undergo glycosylation in the ER and Golgi apparatus. Mutations in GMPPA can cause AAMR syndrome with various symptoms. Loss of GMPPA leads to Golgi fragmentation, altered protein abundance, reduced furin activity, and increased retention of alpha-DG.
Review
Biochemistry & Molecular Biology
Danette Kowal Seiler, Jesse C. Hay
Summary: Fluorescent proteins (FPs) have become essential tools for studying the constitutive secretory pathway in live cells since their discovery over fifty years ago. Genetically encoded chemical tags and innovative methods have also contributed to the understanding of sequential trafficking steps in the secretory pathway.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Rebecca J. J. Taylor, Grigory Tagiltsev, John A. G. Briggs
Summary: COPI-coated vesicles are involved in transport between Golgi stacks and retrograde transport from the Golgi to the endoplasmic reticulum. This process is regulated by small GTPases of the Arf family, which recruit the COPI coat to the membrane for vesicle formation. Understanding the interplay between coatomer, Arf GTPases, and their effectors is essential to comprehend the mechanism of COPI-coated vesicle trafficking.
Article
Cell Biology
Elena Morelli, Elisa A. Speranza, Enrica Pellegrino, Galina Beznoussenko, Francesca Carminati, Massimiliano Garre, Alexander A. Mironov, Marco Onorati, Thomas Vaccari
Summary: Snap29 is a crucial regulator of membrane fusion, essential for cellular processes such as autophagy and cell division. Mutations in Snap29 can lead to alterations in Golgi apparatus and disrupted ER to GA trafficking, revealing a new regulatory function of Snap29 in promoting secretory trafficking.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nuomin Li, Yunjie Qiu, Hao Wang, Juan Zhao, Hong Qing
Summary: Neuritic plaques, major pathological features of Alzheimer's disease, are formed by the aggregation of A beta protein derived from APP cleavage by BACE1 and gamma-secretase, with mutations in PSEN1 gene affecting the activity of gamma-secretase and contributing to early onset familial Alzheimer's disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Cell Biology
Hana Popelka, Daniel J. J. Klionsky
Summary: A recent study used cryo-electron tomography combined with computational analysis to gain insights into autophagosome biogenesis in yeast cells. This approach provided new information on autophagic structures, their contacts with organelles, membrane sources, and transition mechanisms. These findings open new avenues for autophagy research and highlight the potential of cryo-ET in cell biology.
Article
Plant Sciences
Baiying Li, Yonglun Zeng, Wenhan Cao, Wenxin Zhang, Lixin Cheng, Haidi Yin, Qian Wu, Xiangfeng Wang, Yan Huang, Wilson Chun Yu Lau, Zhong-Ping Yao, Yusong Guo, Liwen Jiang
Summary: The COPII protein complex regulates vesicle transport from the endoplasmic reticulum to the Golgi apparatus in response to plant hormones and abiotic stresses. Plant paralogues are tissue-specifically expressed and finely tuned by phytohormones during various developmental processes.
Article
Cell Biology
Sofia Dimou, Mariangela Dionysopoulou, Georgia Maria Sagia, George Diallinas
Summary: A study has found a non-conventional trafficking route that bypasses the Golgi for transporting nutrient transporters and other important membrane proteins to the fungal plasma membrane. This discovery expands the concept of Golgi-independent biogenesis and suggests that this could be a major and largely overlooked cargo-specific sorting route in eukaryotic cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Clinical Neurology
Maryline Santerre, Sterling P. Arjona, Charles N. S. Allen, Natalia Shcherbik, Bassel E. Sawaya
Summary: The NSPs of the virus play a crucial role in establishing the replication and transcription complex by hijacking the endoplasmic reticulum membrane, potentially leading to central nervous system damage and neurodegeneration. Research on these NSPs will not only reveal their specific role in viral infection but also aid in the discovery of novel targeted drugs.
JOURNAL OF NEUROLOGY
(2021)
Article
Cell Biology
Raffaella Gallo, Arpan Kumar Rai, Alexa B. R. Mcintyre, Katrina Meyer, Lucas Pelkmans
Summary: The dual-specificity kinase DYRK3 plays an important role in regulating the formation and dissolution of biomolecular condensates, affecting processes such as stress recovery and mitotic progression. This study shows that DYRK3 interacts with proteins associated with endoplasmic reticulum exit sites (ERESs), and its inhibition disrupts the organization of the ERES-Golgi interface and secretory trafficking. The regulation of ERES by DYRK3 depends on the N-terminal intrinsically disordered region (IDR) of the peripheral membrane protein SEC16A, which forms liquid-like condensates with ERES components. The findings suggest that the physical state of ERES is crucial for efficient membrane traffic in eukaryotic cells, and DYRK3 phosphorylation plays a role in modulating these material properties.
DEVELOPMENTAL CELL
(2023)
Article
Multidisciplinary Sciences
Ranen Aviner, Kathy H. Li, Judith Frydman, Raul Andino
Summary: This study examines how viral pathogens manipulate host polysomes to synthesize viral proteins and hinder host protein production. By analyzing the interactions between viruses and host cells, the research identifies potential targets for selective antiviral interventions through targeting specialized viral polysomes.
Article
Genetics & Heredity
Meena Balasubramanian, Raja Padidela, Rebecca C. Pollitt, Nicholas J. Bishop, M. Zulf Mughal, Amaka C. Offiah, Bart E. Wagner, Janine McCaughey, David J. Stephens
JOURNAL OF MEDICAL GENETICS
(2018)
Article
Cell Biology
Nicola L. Stevenson, Dylan J. M. Bergen, Amadeus Xu, Emily Wyatt, Freya Henry, Janine McCaughey, Laura Vuolo, Chrissy L. Hammond, David J. Stephens
JOURNAL OF CELL SCIENCE
(2018)
Article
Cell Biology
Janine McCaughey, Nicola L. Stevenson, Stephen Cross, David J. Stephens
JOURNAL OF CELL BIOLOGY
(2019)
Article
Biology
Laura Vuolo, Nicola L. Stevenson, Kate J. Heesom, David J. Stephens
Article
Genetics & Heredity
Meena Balasubramanian, Emma Hobson, Mars Skae, Janine McCaughey, David J. Stephens
MOLECULAR GENETICS & GENOMIC MEDICINE
(2019)
Article
Biochemistry & Molecular Biology
Andreas Gerondopoulos, Helen Strutt, Nicola L. Stevenson, Tomoaki Sobajima, Tim P. Levine, David J. Stephens, David Strutt, Francis A. Barr
Review
Cell Biology
Janine McCaughey, David J. Stephens
TRENDS IN CELL BIOLOGY
(2019)
Article
Cell Biology
Laura Vuolo, Nicola L. Stevenson, Aakash G. Mukhopadhyay, Anthony J. Roberts, David J. Stephens
JOURNAL OF CELL SCIENCE
(2020)
Article
Cell Biology
Nicola L. Stevenson, Dylan J. M. Bergen, Yinhui Lu, M. Esther Prada-Sanchez, Karl E. Kadler, Chrissy L. Hammond, David J. Stephens
Summary: Knockout of the golgin giantin leads to skeletal and craniofacial defects due to changes in glycosylation and extracellular matrix deposition. Our study reveals a conserved role for giantin in collagen biosynthesis and extracellular matrix assembly, as well as a giantin-dependent pathway for intracellular procollagen processing.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Janine McCaughey, Nicola L. Stevenson, Judith M. Mantell, Chris R. Neal, Alex Paterson, Kate Heesom, David J. Stephens
Summary: The MIA3 gene encodes two major forms of the TANGO1 protein, which play important roles in maintaining the structure and function of the secretory pathway. Disruption of TANGO1L has relatively minor effects, while loss of both long and short forms results in major defects in cell organization and secretion.
JOURNAL OF CELL SCIENCE
(2021)
Review
Cell Biology
John Hellicar, Nicola L. Stevenson, David J. Stephens, Martin Lowe
Summary: The biomechanical and biochemical properties of connective tissues are determined by the composition and quality of their extracellular matrix, which are highly influenced by the function and organization of the Golgi complex. Dysfunction of the Golgi has been found to have a significant impact on connective tissue in terms of disease and aging.
JOURNAL OF CELL SCIENCE
(2022)
Article
Cell Biology
Caroline Shak, Laura Vuolo, Borhan Uddin, Yohei Katoh, Tom Brown, Aakash G. Mukhopadhyay, Kate Heesom, Anthony J. Roberts, Nicola Stevenson, Kazuhisa Nakayama, David J. Stephens
Summary: The primary cilium is a sensory organelle that receives signals from the external environment and transports them into the cell. Mutations in proteins required for transport in the primary cilium result in ciliopathies, which lead to the malformation of organs and skeletal dysplasias. WDR34, a dynein-2 intermediate chain, is necessary for the maintenance of cilia function.
JOURNAL OF CELL SCIENCE
(2023)
Article
Cell Biology
Shunya Hiyamizu, Hantian Qiu, Laura Vuolo, Nicola L. Stevenson, Caroline Shak, Kate J. Heesom, Yuki Hamada, Yuta Tsurumi, Shuhei Chiba, Yohei Katoh, David J. Stephens, Kazuhisa Nakayama
Summary: The dynein-2 complex is transported anterogradely within cilia to drive retrograde trafficking of the intraflagellar transport (IFT) machinery containing IFT-A and IFT-B complexes. There are multiple interactions between the dynein-2 and IFT-B subunits, including WDR60 and the DYNC2H1-DYNC2LI1 dimer from dynein-2, and IFT54 and IFT57 from IFT-B. These interactions play a crucial role in the connection between dynein-2 and IFT-B.
JOURNAL OF CELL SCIENCE
(2023)
Letter
Medicine, Research & Experimental
Julia Etich, Oliver Semler, Nicola L. Stevenson, Alice Stephan, Roberta Besio, Nadia Garibaldi, Nadine Reintjes, Claudia Dafinger, Max Christoph Liebau, Ulrich Baumann, Matthias Moergelin, Antonella Forlino, David J. Stephens, Christian Netzer, Frank Zaucke, Mirko Rehberg
Summary: This study extends the range of TAPT1 mutations causing osteogenesis imperfecta (OI) and connects extracellular matrix changes to signaling regulation.
EMBO MOLECULAR MEDICINE
(2023)