Article
Biochemistry & Molecular Biology
Friedrich Braumann, Dennis Klug, Jessica Kehrer, Gaojie Song, Juan Feng, Timothy A. Springer, Friedrich Frischknecht
Summary: Eukaryotic cell adhesion and migration rely on surface adhesins connecting extracellular ligands to the intracellular actin cytoskeleton. Plasmodium sporozoites utilize adhesion and gliding motility for transmission and colonization purposes. The role of the TRAP adhesin and the importance of its conformational states in sporozoite behavior were studied, revealing that dynamic conformational change is required for ligand binding, gliding motility, and organ invasion, thus impacting sporozoite transmission.
Article
Multidisciplinary Sciences
Runchen Zhao, Siqi Cui, Zhuoxu Ge, Yuqi Zhang, Kaustav Bera, Lily Zhu, Sean X. Sun, Konstantinos Konstantopoulos
Summary: Cells transitioning from amoeboid to mesenchymal phenotypes in response to increasing hydraulic resistance exhibit oscillatory changes in actin network formation and focal adhesion maturation. Blocking the mechanosensor TRPM7 eliminates the dependence of transition period on resistance, and mathematical modeling reveals the role of intracellular calcium oscillations in this process. Hydraulic resistance emerges as a critical physical factor influencing cell phenotype, with a proposed framework for linking fluorescence signal fluctuations to morphological changes.
Article
Cell Biology
Daniel Feng, Peng Gao, Nathalie Henley, Marion Dubuissez, Nan Chen, Louis-Philippe Laurin, Virginie Royal, Vincent Pichette, Casimiro Gerarduzzi
Summary: SMOC2 is highly expressed in RCC tissue and is associated with shorter patient survival. SMOC2 induces EMT in kidney epithelial cells, promoting RCC metastasis. Mechanistically, SMOC2 activates EMT through the integrin beta 3, FAK, and paxillin pathway. In vivo studies demonstrate that SMOC2 overexpression leads to increased tumor growth and metastatic potential.
CELL DEATH & DISEASE
(2022)
Article
Multidisciplinary Sciences
Christopher L. Yankaskas, Kaustav Bera, Konstantin Stoletov, Selma A. Serra, Julia Carrillo-Garcia, Soontorn Tuntithavornwat, Panagiotis Mistriotis, John D. Lewis, Miguel A. Valverde, Konstantinos Konstantopoulos
Summary: The study reveals a molecular mechanism by which cells avoid high shear during intravasation by activating TRPM7 channel to promote extracellular calcium influx, which then activates different pathways to coordinate the reversal of migration direction and avoid shear stress. Cells with higher TRPM7 activity levels intravasate less efficiently and establish less invasive metastatic lesions. This study provides a mechanistic interpretation for the role of shear stress and its sensor, TRPM7, in tumor cell intravasation.
Article
Immunology
Mark E. Snyder, Kaveh Moghbeli, Anna Bondonese, Andrew Craig, Iulia Popescu, Li Fan, Tracy Tabib, Robert Lafyatis, Kong Chen, Humberto E. Trejo Bittar, Elizabeth Lendermon, Joseph Pilewski, Bruce Johnson, Silpa Kilaru, Yingze Zhang, Pablo G. Sanchez, Jonathan K. Alder, Peter A. Sims, John F. McDyer
Summary: During acute cellular rejection after lung transplantation, there is an intra-allograft oligoclonal expansion of cytotoxic CD8(+) T cells. Despite treatment with systemic glucocorticoids, expanded T cell clones persist as transcriptionally reprogrammed, airway-centric tissue resident memory T cells.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Tamaghna Gupta, Rakesh P. P. Sahu, Mohammadhossein Dabaghi, Lily Shengjia Zhong, Yaron Shargall, Jeremy A. A. Hirota, Carl D. D. Richards, Ishwar K. K. Puri
Summary: Soluble signaling molecules and extracellular matrix (ECM) play a crucial role in regulating cell dynamics. In this study, a non-destructive magnetic exclusion technique is used to form annular aggregates of bronchial epithelial cells on different surfaces. The effects of various signaling molecules on cell dynamics are investigated, and the topography and wettability of the surfaces are measured. The magnetic exclusion-based assay provides a rapid and versatile alternative to traditional wound healing assays.
Article
Chemistry, Physical
Neha Paddillaya, Kalyani Ingale, Chaitanya Gaikwad, Deepak Kumar Saini, Pramod Pullarkat, Paturu Kondaiah, Gautam Menon, Namrata Gundiah
Summary: This study quantified the critical adhesion strengths and cell tractions of different cell types and found that they were inversely correlated with cell invasion potentials. The results showed higher traction stresses in invasive cells compared to non-invasive cells. Immunofluorescence studies revealed differences in the distribution of the cytoskeleton and vinculin. These findings are important for cancer diagnostics and research on adhesion-related pathologies.
Article
Physics, Multidisciplinary
Macia Esteve Pallares, Irina Pi-Jauma, Isabela Corina Fortunato, Valeria Grazu, Manuel Gomez-Gonzalez, Pere Roca-Cusachs, Jesus M. de la Fuente, Ricard Alert, Raimon Sunyer, Jaume Casademunt, Xavier Trepat
Summary: The migratation of cellular clusters is important for various biological processes, but the mechanisms underlying collective durotaxis are not fully understood. This study reveals a connection between collective durotaxis and the wetting properties of cellular clusters. The findings show that the wetting behavior of cancer cells on substrates is dependent on the stiffness of the substrates. The study also demonstrates a physical mechanism of collective durotaxis based on the wetting properties of active droplets.
Article
Biochemical Research Methods
Emmet A. Francis, Volkmar Heinrich
Summary: The dynamic interplay between cell adhesion and protrusion is crucial for cell motility. Traditional mathematical models often consider passive cell adhesion as the primary driving force for cell spreading, but this is inconsistent with experimental results. In contrast, a protrusive zipper model agrees well with experimental findings and can reproduce the observed correlation between antibody density and maximum cell-substrate contact area. This integrative experimental/computational approach reveals that phagocytic spreading is driven by cellular protrusion and is limited by the density of adhesion sites.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Camila Ignacio da Silva, Cassiano Felippe Goncalves-de-Albuquerque, Bianca Portugal Tavares de Moraes, Diogo Gomes Garcia, Patricia Burth
Summary: NKA is a p-type transmembrane enzyme formed by three different subunits and plays a crucial role in transporting sodium and potassium through the cell membrane as well as intracellular signaling. Changes in NKA function can impact pathological conditions like cancer, affecting cell adhesion, motility, and migration.
Article
Physics, Fluids & Plasmas
C. Alex Safsten, Volodmyr Rybalko, Leonid Berlyand
Summary: This study focuses on the onset of motion of a living cell driven by myosin contraction. The results show that stable asymmetric moving states bifurcate from unstable radial stationary states, and these moving states have nonlinear asymptotic stability in modeling observable steady cell motion.
Article
Cell Biology
Victoria Levario-Diaz, Rebecca Elizabeth Alvarado, Cristina Marcela Rodriguez-Quinteros, Andreas Fink, Joel Christian, Wenqian Feng, Elisabetta Ada Cavalcanti-Adam
Summary: This study describes a novel method to fabricate 1D micro-nanopatterned stripes, and reveals the migratory behavior of fibroblasts in 1D environments by regulating integrin clustering and focal adhesion dynamics.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Physics, Multidisciplinary
Guanming Zhang, Julia M. Yeomans
Summary: We studied the influence of intercellular active forces on individual cell morphology and collective motion in a cell monolayer using a computational phase-field model and analytical analysis. We focused on the regime where intercellular forces dominate tissue dynamics and polar forces are negligible. Our results showed that contractile intercellular interactions lead to cell elongation and nematic ordering, resulting in active turbulence with motile topological defects. On the other hand, extensile interactions resulted in frustration and increased prevalence of perpendicular cell orientations. Furthermore, we found that contractile behavior can transition to extensile behavior when considering anisotropic fluctuations in cell shape.
PHYSICAL REVIEW LETTERS
(2023)
Article
Multidisciplinary Sciences
Nabila Founounou, Reza Farhadifar, Giovanna M. Collu, Ursula Weber, Michael J. Shelley, Marek Mlodzik
Summary: The study highlights the critical role of the Nemo molecule in regulating the transition from solid to viscoelastic tissues in the process of cell migration, emphasizing the importance of junctional remodeling regulation for tissue fluidity.
NATURE COMMUNICATIONS
(2021)
Review
Oncology
Dolly Dhaliwal, Trevor G. Shepherd
Summary: Epithelial ovarian cancer (EOC) is the most lethal gynecological malignancy in the developed world, with a unique metastasis process involving cell detachment and formation of spheroids. Integrins, a family of cell adhesion receptors, play a crucial role in EOC metastasis impacting various steps of cancer progression, suggesting a potential therapeutic target for advanced EOC.
CLINICAL & EXPERIMENTAL METASTASIS
(2022)
Article
Engineering, Biomedical
Charlotte Alibert, David Pereira, Nathan Lardier, Sandrine Etienne-Manneville, Bruno Goud, Atef Asnacios, Jean-Baptiste Manneville
Summary: Cells tend to soften during cancer progression, and mechanical phenotyping could be used as a diagnostic or prognostic method. By investigating the cell mechanics of gliomas, research shows that cell mechanics discriminates human glioma cells of different grades, suggesting the potential of combining rheology techniques for cancer cell mechanophenotyping in diagnostic or prognostic methods.
Review
Cell Biology
Emma J. van Bodegraven, Sandrine Etienne-Manneville
Summary: Cytoplasmic intermediate filaments play a key role in integrating cell and tissue mechanics, allowing cells to adapt to mechanical challenges. Changes in IF gene expression, network assembly dynamics, and post-translational modifications can tune the mechanical properties of cells and tissues to changing environments.
Article
Chemistry, Physical
Shailaja Seetharaman, Benoit Vianay, Vanessa Roca, Aaron J. Farrugia, Chiara De Pascalis, Batiste Boeda, Florent Dingli, Damarys Loew, Stephane Vassilopoulos, Alexander Bershadsky, Manuel Thery, Sandrine Etienne-Manneville
Summary: Mechanotransduction is a process where cells sense mechanical properties of their environment, with integrin-mediated focal adhesions being crucial sites. The crosstalk between microtubules and actin in mechanotransduction affects cell adhesion and migration. Substrate-rigidity-dependent microtubule acetylation controls YAP translocation, focal adhesion distribution, actomyosin contractility, and cell migration.
Article
Microbiology
Yanlei Zhu, Flavio Alvarez, Nicolas Wolff, Ariel Mechaly, Sebastien Brule, Benoit Neitthoffer, Sandrine Etienne-Manneville, Ahmed Haouz, Batiste Boeda, Celia Caillet-Saguy
Summary: The C-terminus of the SARS-CoV-2 protein E contains a PDZ-binding motif (PBM) that interacts with human PDZ-containing proteins involved in cellular junctions and polarity. Crystal structures of complexes between human LNX2, MLLT4, and MPP5 PDZs and SARS-CoV-2 E PBM were solved, revealing the binding preferences of the PBM for cellular targets. Mutations in the E protein near the PBM may have important effects on its structure and ion-channel activity, as well as the host machinery targeted by the variants during infection.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Filipe Nunes Vicente, Mickael Lelek, Jean-Yves Tinevez, Quang D. Tran, Gerard Pehau-Arnaudet, Christophe Zimmer, Sandrine Etienne-Manneville, Gregory Giannone, Cecile Leduc
Summary: Intermediate filaments play key roles in cellular functions and their mechanical properties. This study used super-resolution microscopy to reveal the molecular organization of vimentin filaments and provided evidence for the partial overlap of subunits during filament assembly. The researchers also found that the extensibility of vimentin is due to the unfolding of its subunits, establishing a direct link between structure and mechanics.
Article
Biology
Stephanie Portet, Sandrine Etienne-Manneville, Cecile Leduc, J. C. Dallon
Summary: This research investigates the effect of noise on the transport of intermediate filaments in cells and finds that noise promotes collective movement and enhances regulation efficiency of intermediate filaments through biochemical properties. The study reveals the impact of stochastic fluctuations on individual and ensemble transport.
JOURNAL OF THEORETICAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Florent Peglion, Lavinia Capuana, Isabelle Perfettini, Laurent Boucontet, Ben Braithwaite, Emma Colucci-Guyon, Emie Quissac, Karin Forsberg-Nilsson, Flora Llense, Sandrine Etienne-Manneville
Summary: Loss of PTEN promotes cell migration while targeting AMPK activity can counteract this effect. This suggests the important regulatory roles of PTEN and AMPK in tumor invasion and provides new therapeutic opportunities for glioblastoma.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Florent Peglion, Franck Coumailleau, Sandrine Etienne-Manneville
Summary: Glioblastoma (GBM) is a devastating malignant brain tumor characterized by its invasive properties, leading to treatment failure. This study presents a physiological model in which GBM cells are orthotopically xenografted into the zebrafish brain, allowing for subcellular intravital live imaging and analysis of microtubule dynamics during GBM invasion. The findings contribute to a better understanding of GBM motility and could potentially lead to the discovery of new targets for blocking tumor infiltration and improving patient outcomes.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Review
Cell Biology
Elvira Infante, Sandrine Etienne-Manneville
Summary: Cell migration is a dynamic process crucial for the development of multicellular organisms and immune system responses. The cytoskeletal component known as intermediate filaments plays a key role in cell migration by integrating mechanical properties and signaling functions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Abhijit Deb Roy, Evan G. Gross, Gayatri S. Pillai, Shailaja Seetharaman, Sandrine Etienne-Manneville, Takanari Inoue
Summary: The subcellular localization and microtubule acetylation levels of alpha-TAT1 are determined by its disordered region containing three functional elements. The dynamic intracellular localization of alpha-TAT1 and its catalytic activity play a crucial role in microtubule acetylation efficiency. The allosteric spatial regulation of alpha-TAT1 function may uncover a spatiotemporal code of microtubule acetylation in normal and aberrant cell behavior.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biochemical Research Methods
J. C. Dallon, Cecile Leduc, Christopher P. Grant, Emily J. Evans, Sandrine Etienne-Manneville, Stephanie Portet
Summary: Fluorescence Recovery After Photobleaching (FRAP) has been extensively used to understand molecular dynamics in cells. However, a new mathematical approach is needed to analyze FRAP data for anisotropic structures subjected to directed transport, such as cytoskeletal filaments or elongated organelles transported along microtubule tracks.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Review
Cell Biology
Chenglin Miao, Shuangshuang Zhao, Sandrine Etienne-Manneville, Yaming Jiu
Summary: Bacterial infection poses a significant threat to human health, necessitating a deeper understanding of bacteria-host interactions. Vimentin, an intermediate filament protein, plays a vital role during bacterial infection by interacting with host cytoskeletal networks. It can be found in the cytoplasm, secreted, or on the cell surface, and its presence influences bacterial invasion and survival, as well as bacterial-induced inflammation regulation. Bacteria can also manipulate vimentin in host cells. Targeting vimentin with drugs may offer a synergistic advantage as an anti-bacterial therapeutic approach.
JOURNAL OF CELL SCIENCE
(2023)
Review
Cell Biology
Florent Peglion, Sandrine Etienne-Manneville
Summary: The authors discuss the role of cell polarity alteration in cancer development and its potential application in cancer prediction, diagnosis, and treatment. Abnormal polarity is a hallmark of cancer cells, while healthy cells and tissues have well-organized cell polarity. Key evolutionarily conserved proteins frequently undergo alterations in cancer, but their specific roles in oncogenic processes are not always clear.
JOURNAL OF CELL BIOLOGY
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
Abhijit Deb Roy, Evan G. Gross, Gayatri S. Pillai, Shailaja Seetharaman, Sandrine Etienne-Manneville, Takanari Inoue
CIRCULATION RESEARCH
(2021)
