A standardized workflow for surveying recombinases expands bacterial genome-editing capabilities

The Ssr protein (T1E_1405) fromPseudomonas putidaDOT-T1E enables oligonucleotide-based recombineering in platform strainP. putidaEM42

(2016) Tomás Aparicio et al.   Biotechnology Journal

A highly precise and portable genome engineering method allows comparison of mutational effects across bacterial species

(2016) Ákos Nyerges et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Ssr protein (T1E_1405) fromPseudomonas putidaDOT-T1E enables oligonucleotide-based recombineering in platform strainP. putidaEM42

(2016) Tomás Aparicio et al.   Biotechnology Journal

A high-efficiency recombineering system with PCR-based ssDNA in Bacillus subtilis mediated by the native phage recombinase GP35

(2015) Zhaopeng Sun et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Pseudomonas putida—a versatile host for the production of natural products

(2015) Anita Loeschcke et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Pseudomonas putidaKT2440 Strain Metabolizes Glucose through a Cycle Formed by Enzymes of the Entner-Doudoroff, Embden-Meyerhof-Parnas, and Pentose Phosphate Pathways

(2015) Pablo I. Nikel et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Transient overexpression of DNA adenine methylase enables efficient and mobile genome engineering with reduced off-target effects

(2015) Rebecca M. Lennen et al.   NUCLEIC ACIDS RESEARCH

FreeingPseudomonas putida KT2440 of its proviral load strengthens endurance to environmental stresses

(2014) Esteban Martínez-García et al.   ENVIRONMENTAL MICROBIOLOGY

Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression

(2014) Esteban Martínez-García et al.   Microbial Cell Factories

Rapid editing and evolution of bacterial genomes using libraries of synthetic DNA

(2014) Ryan R Gallagher et al.   Nature Protocols

Biotechnological domestication of pseudomonads using synthetic biology

(2014) Pablo I. Nikel et al.   NATURE REVIEWS MICROBIOLOGY

CRISPR–Cas9-assisted recombineering in Lactobacillus reuteri

(2014) Jee-Hwan Oh et al.   NUCLEIC ACIDS RESEARCH

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Recombineering in Corynebacterium glutamicum combined with optical nanosensors: a general strategy for fast producer strain generation

(2013) Stephan Binder et al.   NUCLEIC ACIDS RESEARCH

Industrial biotechnology of Pseudomonas putida and related species

(2012) Ignacio Poblete-Castro et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes

(2012) Rafael Silva-Rocha et al.   NUCLEIC ACIDS RESEARCH

Improving Lambda Red Genome Engineering in Escherichia coli via Rational Removal of Endogenous Nucleases

(2012) Joshua A. Mosberg et al.   PLoS One

pBAM1: an all-synthetic genetic tool for analysis and construction of complex bacterial phenotypes

(2011) Esteban Martínez-García et al.   BMC MICROBIOLOGY

Engineering multiple genomic deletions in Gram-negative bacteria: analysis of the multi-resistant antibiotic profile of Pseudomonas putida KT2440

(2011) Esteban Martínez-García et al.   ENVIRONMENTAL MICROBIOLOGY

Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement

(2011) Farren J. Isaacs et al.   SCIENCE

Recombineering Using RecTE from Pseudomonas syringae

(2010) B. Swingle et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Scarless and sequential gene modification in Pseudomonas using PCR product flanked by short homology regions

(2010) Rubing Liang et al.   BMC MICROBIOLOGY

Detection of novel recombinases in bacteriophage genomes unveils Rad52, Rad51 and Gp2.5 remote homologs

(2010) Anne Lopes et al.   NUCLEIC ACIDS RESEARCH

Rapid Oligonucleotide-Based Recombineering of the Chromosome of Salmonella enterica

(2009) R. G. Gerlach et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Molecular characterization of Rifr mutations in Pseudomonas aeruginosa and Pseudomonas putida

(2009) Tatjana Jatsenko et al.   MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Efficient point mutagenesis in mycobacteria using single-stranded DNA recombineering: characterization of antimycobacterial drug targets

(2008) Julia C. van Kessel et al.   MOLECULAR MICROBIOLOGY

Identification and analysis of recombineering functions from Gram-negative and Gram-positive bacteria and their phages

(2008) S. Datta et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Tracing explosives in soil with transcriptional regulators of Pseudomonas putida evolved for responding to nitrotoluenes

(2008) Junkal Garmendia et al.   Microbial Biotechnology

Genome-Scale Reconstruction and Analysis of the Pseudomonas putida KT2440 Metabolic Network Facilitates Applications in Biotechnology

(2008) Jacek Puchałka et al.   PLoS Computational Biology