Transient overexpression of DNA adenine methylase enables efficient and mobile genome engineering with reduced off-target effects

Rational design of a synthetic Entner–Doudoroff pathway for improved and controllable NADPH regeneration

(2015) Chiam Yu Ng et al.   METABOLIC ENGINEERING

Site-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway

(2015) Jae Seong Lee et al.   Scientific Reports

Combinatorial Strategies for Improving Multiple-Stress Resistance in Industrially Relevant Escherichia coli Strains

(2014) Rebecca M. Lennen et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Evolved Osmotolerant Escherichia coli Mutants Frequently Exhibit DefectiveN-Acetylglucosamine Catabolism and Point Mutations in Cell Shape-Regulating Protein MreB

(2014) James D. Winkler et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Evolution of Escherichia coli to 42 °C and Subsequent Genetic Engineering Reveals Adaptive Mechanisms and Novel Mutations

(2014) Troy E. Sandberg et al.   MOLECULAR BIOLOGY AND EVOLUTION

MODEST: a web-based design tool for oligonucleotide-mediated genome engineering and recombineering

(2014) Mads T. Bonde et al.   NUCLEIC ACIDS RESEARCH

Conditional DNA repair mutants enable highly precise genome engineering

(2014) Ákos Nyerges et al.   NUCLEIC ACIDS RESEARCH

Prediction of resistance development against drug combinations by collateral responses to component drugs

(2014) C. Munck et al.   Science Translational Medicine

Direct Mutagenesis of Thousands of Genomic Targets Using Microarray-Derived Oligonucleotides

(2014) Mads T. Bonde et al.   ACS Synthetic Biology

Probing the Limits of Genetic Recoding in Essential Genes

(2013) M. J. Lajoie et al.   SCIENCE

Genomically Recoded Organisms Expand Biological Functions

(2013) M. J. Lajoie et al.   SCIENCE

Newly Identified Genetic Variations in Common Escherichia coli MG1655 Stock Cultures

(2011) P. L. Freddolino et al.   JOURNAL OF BACTERIOLOGY

Modified bases enable high-efficiency oligonucleotide-mediated allelic replacement via mismatch repair evasion

(2011) H. H. Wang et al.   NUCLEIC ACIDS RESEARCH

Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement

(2011) Farren J. Isaacs et al.   SCIENCE

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE