The Ssr protein (T1E_1405) fromPseudomonas putidaDOT-T1E enables oligonucleotide-based recombineering in platform strainP. putidaEM42

MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets

(2016) Sudhir Kumar et al.   MOLECULAR BIOLOGY AND EVOLUTION

A highly precise and portable genome engineering method allows comparison of mutational effects across bacterial species

(2016) Ákos Nyerges et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Pseudomonas putida—a versatile host for the production of natural products

(2015) Anita Loeschcke et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Pseudomonas putidaKT2440 Strain Metabolizes Glucose through a Cycle Formed by Enzymes of the Entner-Doudoroff, Embden-Meyerhof-Parnas, and Pentose Phosphate Pathways

(2015) Pablo I. Nikel et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Transient overexpression of DNA adenine methylase enables efficient and mobile genome engineering with reduced off-target effects

(2015) Rebecca M. Lennen et al.   NUCLEIC ACIDS RESEARCH

A functional recT gene for recombineering of Clostridium

(2014) Hongjun Dong et al.   JOURNAL OF BIOTECHNOLOGY

Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression

(2014) Esteban Martínez-García et al.   Microbial Cell Factories

Rapid editing and evolution of bacterial genomes using libraries of synthetic DNA

(2014) Ryan R Gallagher et al.   Nature Protocols

Biotechnological domestication of pseudomonads using synthetic biology

(2014) Pablo I. Nikel et al.   NATURE REVIEWS MICROBIOLOGY

Conditional DNA repair mutants enable highly precise genome engineering

(2014) Ákos Nyerges et al.   NUCLEIC ACIDS RESEARCH

SEVA 2.0: an update of the Standard European Vector Architecture for de-/re-construction of bacterial functionalities

(2014) Esteban Martínez-García et al.   NUCLEIC ACIDS RESEARCH

CRISPR–Cas9-assisted recombineering in Lactobacillus reuteri

(2014) Jee-Hwan Oh et al.   NUCLEIC ACIDS RESEARCH

Direct Mutagenesis of Thousands of Genomic Targets Using Microarray-Derived Oligonucleotides

(2014) Mads T. Bonde et al.   ACS Synthetic Biology

MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability

(2013) K. Katoh et al.   MOLECULAR BIOLOGY AND EVOLUTION

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Recombineering in Corynebacterium glutamicum combined with optical nanosensors: a general strategy for fast producer strain generation

(2013) Stephan Binder et al.   NUCLEIC ACIDS RESEARCH

Metabolic potential of the organic-solvent tolerantPseudomonas putida DOT-T1E deduced from its annotated genome

(2013) Zulema Udaondo et al.   Microbial Biotechnology

Industrial biotechnology of Pseudomonas putida and related species

(2012) Ignacio Poblete-Castro et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

The Entner-Doudoroff pathway empowersPseudomonas putida KT2440 with a high tolerance to oxidative stress

(2012) Max Chavarría et al.   ENVIRONMENTAL MICROBIOLOGY

High efficiency recombineering in lactic acid bacteria

(2012) Jan-Peter van Pijkeren et al.   NUCLEIC ACIDS RESEARCH

The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes

(2012) Rafael Silva-Rocha et al.   NUCLEIC ACIDS RESEARCH

Improving Lambda Red Genome Engineering in Escherichia coli via Rational Removal of Endogenous Nucleases

(2012) Joshua A. Mosberg et al.   PLoS One

Substrate and Target Sequence Length Influence RecTEPsy Recombineering Efficiency in Pseudomonas syringae

(2012) Zhongmeng Bao et al.   PLoS One

Multiplexed in Vivo His-Tagging of Enzyme Pathways for in Vitro Single-Pot Multienzyme Catalysis

(2012) Harris H. Wang et al.   ACS Synthetic Biology

Exploring optimization parameters to increase ssDNA recombineering inLactococcus lactisandLactobacillus reuteri

(2012) Jan-Peter van Pijkeren et al.   Bioengineered

pBAM1: an all-synthetic genetic tool for analysis and construction of complex bacterial phenotypes

(2011) Esteban Martínez-García et al.   BMC MICROBIOLOGY

Engineering multiple genomic deletions in Gram-negative bacteria: analysis of the multi-resistant antibiotic profile of Pseudomonas putida KT2440

(2011) Esteban Martínez-García et al.   ENVIRONMENTAL MICROBIOLOGY

Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement

(2011) Farren J. Isaacs et al.   SCIENCE

Recombineering Using RecTE from Pseudomonas syringae

(2010) B. Swingle et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Scarless and sequential gene modification in Pseudomonas using PCR product flanked by short homology regions

(2010) Rubing Liang et al.   BMC MICROBIOLOGY

Molecular characterization of Rifr mutations in Pseudomonas aeruginosa and Pseudomonas putida

(2009) Tatjana Jatsenko et al.   MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

The dif/Xer Recombination Systems in Proteobacteria

(2009) Christophe Carnoy et al.   PLoS One

Use of the lambda Red recombinase system to rapidly generate mutants in Pseudomonas aeruginosa

(2008) Biliana Lesic et al.   BMC MOLECULAR BIOLOGY

Identification and analysis of recombineering functions from Gram-negative and Gram-positive bacteria and their phages

(2008) S. Datta et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA