Article
Oncology
Pan Wang, Sheng Gong, Bin Liao, Jinyu Pan, Junwei Wang, Dewei Zou, Lu Zhao, Shuanglong Xiong, Yangmin Deng, Qian Yan, Nan Wu
Summary: The study demonstrated that in a hypoxic microenvironment, the HIF1 alpha/HIF2 alpha-Sox2 network induced the formation of glioma stem cells through the dedifferentiation of differentiated glioma cells, thus promoting glioma cell chemoresistance. Both HIF1 alpha and HIF2 alpha, as genes upstream of Sox2, regulate the malignant progression of glioma through dedifferentiation. The knockout of HIF1 alpha, HIF2 alpha, and Sox2 in glioma cells under hypoxic conditions led to decreased CD133 and CD15 expression and inhibited sphere formation.
Article
Chemistry, Multidisciplinary
Huan Li, Chao Song, Yang Zhang, Guohao Liu, Hailong Mi, Yachao Li, Zhiye Chen, Xiaoyu Ma, Po Zhang, Lidong Cheng, Peng Peng, Hongtao Zhu, Zirong Chen, Minhai Dong, Sui Chen, Hao Meng, Qungen Xiao, Honglian Li, Qiulian Wu, Baofeng Wang, Suojun Zhang, Kai Shu, Feng Wan, Dongsheng Guo, Wenchao Zhou, Lin Zhou, Feng Mao, Jeremy N. Rich, Xingjiang Yu
Summary: Hypoxia induces the expression of actin-binding protein Transgelin (TAGLN) in Glioblastoma stem cells (GSCs), promoting their survival and self-renewal through the regulation of HIF1α and stabilization of HDAC2. Sodium valproate (VPA), a specific inhibitor of TAGLN/HDAC2 function, shows enhanced efficacy when combined with natural borneol (NB) in vivo.
Article
Biochemistry & Molecular Biology
Parveen Kumar, Vivek Verma, Dheeraj Mohania, Surbhi Gupta, Avneet K. Babbar, Bhawna Rathi, Rakesh S. Dhanda, Manisha Yadav
Summary: The study revealed that RUNX1T1 is mutated in different cancers and associated with poor overall survival in glioma patients, possibly affecting glioma growth and development through HIF1α regulation. Experimental results showed that downregulation of RUNX1T1 may lead to degradation of HIF1α, reducing proliferation and invasiveness of glioblastoma cells.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Wenjing Tu, Hui Zheng, Liangdong Li, Changshuai Zhou, Mingtao Feng, Lei Chen, Deheng Li, Xin Chen, Bin Hao, Huaping Sun, Yiqun Cao, Yang Gao
Summary: This study demonstrates that the GBM-secreted cytokine SPP1 regulates the expression of PSMA in human umbilical vein endothelial cells (HUVECs) through the transcription factor HIF1 alpha, and promotes cell migration and tube formation. SPP1 is abundantly expressed in GBM, associated with poor prognosis, and has high clinical diagnostic value. These findings provide insight into the angiogenic process and promising candidates for targeted GBM therapy.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2023)
Article
Genetics & Heredity
Shuanglong Xiong, Donglin Wang, Yin Tang, Songmei Lu, Lumi Huang, Zhijuan Wu, Shuangyi Lei, Guanzhong Liang, Dan Yang, Dairong Li, Yan Li
Summary: Objective: To investigate the role of HIF1 alpha and HIF2 alpha in regulating the dedifferentiation of lung cancer cells under hypoxic conditions through Sox2 and Oct4. Methods: The expression of HIF1 alpha, HIF2 alpha, Sox2, and Oct4 was analyzed in lung cancer tissues. Sphere formation and the expression of CD133, CD44, Sox2, Oct4, HIF1 alpha, and HIF2 alpha were examined in differentiated lung cancer cells cultured under hypoxia. Knockout experiments were performed for HIF1 alpha, HIF2 alpha, Sox2, and Oct4, and the effects on CD133 and CD44 expression were evaluated. Results: Differentiated lung cancer cells formed more spheres under hypoxic conditions and exhibited higher expression of CD133 and CD44. TCGA database analysis revealed elevated levels of HIF1 alpha and HIF2 alpha in lung cancer tissues. Knockout of HIF1 alpha and HIF2 alpha resulted in decreased expression of Sox2, Oct4, CD133, and CD44, while knockout of Sox2 or Oct4 led to decreased expression of CD133 and CD44. Conclusion: HIF1 alpha and HIF2 alpha play a crucial role in regulating the dedifferentiation of non-small-cell lung cancer cells through Sox2 and Oct4 under hypoxic conditions.
Article
Oncology
Ren Liu, Yuanfa Feng, Yulin Deng, Zhihao Zou, Jianheng Ye, Zhiduan Cai, Xuejin Zhu, Yingke Liang, Jianming Lu, Hui Zhang, Yong Luo, Zhaodong Han, Yangjia Zhuo, Qingling Xie, Chi Tin Hon, Yuxiang Liang, Chin-Lee Wu, Weide Zhong
Summary: In clear cell renal cell carcinoma (ccRCC), HIF1 alpha inhibits mitochondrial function and lipid metabolism by directly regulating GPD1, thus impacting tumor activity. This study provides new insights into the treatment of ccRCC and reveals a novel mechanism for the molecular basis of HIF1 alpha inhibition of tumor activity.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Cell Biology
Yingchi Zhang, Shicheng Wang, Jingliao Zhang, Chao Liu, Xinqi Li, Wenbo Guo, Yongjuan Duan, Xiaoyan Chen, Suyu Zong, Jiarui Zheng, Yixuan Wu, Xiaoli Chen, Xuelian Cheng, Yanxia Chang, Yue Wang, Feng Ding, Wenyu Yang, Xiaojuan Chen, Ye Guo, Li Zhang, Yumei Chen, Yao Zou, Xiaofan Zhu, Jin Gu, Tao Cheng
Summary: In this study, Zhang et al. used single-cell transcriptomics and B-cell receptor sequencing to analyze the transcriptomic profiles of B-cell acute lymphoblastic leukaemia cells at diagnosis, residual, and relapse stages. They found that the hypoxia pathway could be a potential target for residual leukaemia cells.
NATURE CELL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Jennifer K. Matsui, Haley K. Perlow, Alex R. Ritter, Rituraj Upadhyay, Raju R. Raval, Evan M. Thomas, Sasha J. Beyer, Clement Pillainayagam, Justin Goranovich, Shirley Ong, Pierre Giglio, Joshua D. Palmer
Summary: This review discusses the treatment modalities for glioblastoma, the mechanisms of radioresistance, and promising radiosensitizers. Small molecules and immunotherapy agents used in conjunction with radiotherapy have been studied in clinical trials. Recent preclinical studies regarding radiosensitizers for glioblastoma are also discussed.
Article
Oncology
Xueran Chen, Wanxiang Niu, Xiaoqing Fan, Haoran Yang, Chenggang Zhao, Junqi Fan, Xuebiao Yao, Zhiyou Fang
Summary: In this study, it was found that Oct4A can impact the tumorigenic activity of glioblastoma and its stability is maintained through palmitoylation. Oct4A palmitoylation helps to integrate Sox4 and Oct4A in the SOX2 enhancement subregion, thereby maintaining the stem performance of glioma stem cells. The study also designed Oct4A palmitoylation competitive inhibitors that can inhibit the self-renewal ability and tumorigenicity of glioma stem cells.
Review
Immunology
Boyuan Huang, Xuesong Li, Yuntao Li, Jin Zhang, Zhitao Zong, Hongbo Zhang
Summary: Glioblastoma multiforme (GBM) is the most common and aggressive malignant tumor in the central nervous system. Immunotherapy has shown promising results in the treatment of GBM, but future developments will require an integrated approach with various treatment modalities and specific targeted therapies for the tumor microenvironment.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Zhijie Zhang, Jiangzhou Shi, Qifang Wu, Zijian Zhang, Xiaoyan Liu, Anqi Ren, Guanlin Zhao, Ge Dong, Han Wu, Jiaxuan Zhao, Yuan Zhao, Jia Hu, Hui Li, Tongcun Zhang, Fuling Zhou, Haichuan Zhu
Summary: A new study reveals that high expression of JUN is associated with poor prognosis in T-ALL patients, and targeting the JNK-JUN-HIF1 alpha axis can enhance the death of Dex-resistant cells, providing a potential therapeutic strategy for T-ALL treatment.
Review
Oncology
Agathe L. Chedeville, Patricia A. Madureira
Summary: Glioblastoma (GB) is the deadliest type of primary brain tumor with a median survival time of 16 months post-diagnosis, and radiotherapy remains the most effective treatment despite the common resistance observed in GB patients. Understanding the role of low tumor oxygenation (hypoxia) in radioresistance is crucial for developing novel strategies to improve tumor cell sensitivity to radiotherapy.
Article
Multidisciplinary Sciences
Qing Guo, Shuai Shen, Gefei Guan, Chen Zhu, Cunyi Zou, Jingyuan Cao, Wen Cheng, Xiaoyan Xu, Juanhan Yu, Zhiguo Lin, Guoli Wang, Ling Chen, Peng Cheng, Anhua Wu
Summary: Glioblastoma (GBM) shares common signaling pathways involving TIM-3, an immune checkpoint, between tumor and non-tumor cells. The study reveals that TIM-3 in glioma cells not only regulates the malignant behaviors of glioma cells but also induces macrophage migration and transition to an anti-inflammatory/protumorigenic phenotype through the TIM-3/IL6 signal. Blocking this feedback loop may provide a novel therapeutic strategy for GBM.
Article
Cell Biology
Zhirui Zeng, Shan Lei, Zhiwei He, Tengxiang Chen, Jianxin Jiang
Summary: Hypoxia is involved in the development of pancreatic cancer, where the key hypoxia-associated gene YEATS2 is upregulated and promotes proliferation and metastasis of PC cells. HIF1α regulates the expression of YEATS2 by binding to its HRE, and both are coexpressed in PC tissues.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Cell Biology
Junxia Li, Yiming Xia, Zhizhong Huang, Yan Zhao, Renping Xiong, Xiaoxu Li, Qingyuan Huang, Fabo Shan
Summary: The study revealed that AECII-derived ISM1 is involved in regulating PMVEC hyperpermeability under hypoxia through the mechanism mediated by HIF1α. This finding highlights the critical role of ISM1 in modulating endothelial permeability in hypoxic conditions via HIF1α regulation.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Review
Clinical Neurology
Wenyan Li, Chenghai Zuo, Hua Feng, Hui Meng
Summary: This study reports the first case of spinal cord atrophy developing 16 months after resection of multiple intraspinal arachnoid cysts. The patient initially experienced back pain, which was successfully treated by cyst resection. However, the back pain recurred 16 months later, and magnetic resonance imaging revealed severe spinal cord atrophy.
BRITISH JOURNAL OF NEUROSURGERY
(2023)
Article
Clinical Neurology
Xiaoke Hao, Zongwei Zeng, Liang Liang, Zhou Feng, Wu Li, Binyuan Xiong, Peiwen Guo, Qiang Zhang, Yujie Chen, Hua Feng, Zhi Chen
Summary: This study found that neutrophil extracellular traps (NETs) play an important role in the formation of microthrombi after subarachnoid hemorrhage (SAH). Depletion of neutrophils and DNase I treatment significantly reduced the formation of NETs and microthrombi, and improved neurological deficits, brain edema, BBB disruption, and neuronal injury after SAH induction.
TRANSLATIONAL STROKE RESEARCH
(2023)
Article
Neurosciences
Min Xia, Yu-Jie Chen, Beike Chen, Xufang Ru, Jie Wang, Jie Lin, Xiaoqin Tang, Weixiang Chen, Rong Hu, Weina Li, Hua Feng
Summary: This study investigates the role of astrocytic transient receptor potential ankyrin 1 (TRPA1) in glial phenotype transformation in neuroinflammation after intracerebral hemorrhage (ICH). The results show that knockout of astrocytic TRPA1 decreases calcium ions of astrocytes and promotes proliferation and phenotypic transformation of astrocytes and microglia. Inhibiting the mitogen-activated protein kinase/nuclear factor kappa-B signaling pathway reduces neuroinflammation and improves neurological deficits. Therefore, astrocytic TRPA1 is a potential therapeutic target for rescuing neuroinflammation after ICH through modulation of glial phenotype.
Article
Medicine, Research & Experimental
Jie Liu, Pan Wang, Chao Tang, Hao-Tian Jiang, Gang Zhang, Nan Wu
Summary: Thanks to rapid development of endoscopic technology, the endoscopic endonasal transsphenoidal approach has become a preferred surgical method for resection of sellar and suprasellar tumors. A report of 21 cases of craniopharyngioma treated with this approach at Chongqing General Hospital showed successful resection with few complications and no recurrence during the follow-up period.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2023)
Article
Clinical Neurology
Haotian Jiang, Dewei Zou, Pan Wang, Longwei Zeng, Jie Liu, Chao Tang, Gang Zhang, Xiaorong Tan, Nan Wu
Summary: Fully endoscopic microvascular decompression is a safe and effective method for the treatment of trigeminal neuralgia, with excellent surgical outcomes and no obvious complications or recurrence after follow-up.
FRONTIERS IN NEUROLOGY
(2023)
Article
Neurosciences
Chuan Shao, Pan Wang, Bin Liao, Sheng Gong, Nan Wu
Summary: Multi-omics expression datasets were used to study the role of TK1 in glioma prognosis. TK1 was overexpressed in glioma and associated with poor overall survival. TK1 expression showed high diagnostic value for predicting glioma survival, and a model incorporating TK1 and clinical characteristics had excellent predictive ability. TK1 alterations were associated with better overall survival, while TK1 showed positive associations with proliferation, cell cycle, DNA repair, DNA damage, and epithelial-mesenchymal transition in glioma. Immunoinfiltration analysis suggested different roles of TK1 in glioma tumor microenvironments.
Article
Biotechnology & Applied Microbiology
Xuejiao Lei, Quan Hu, Hongfei Ge, Xuyang Zhang, Xufang Ru, Yujie Chen, Rong Hu, Hua Feng, Jun Deng, Yan Huang, Wenyan Li
Summary: This study successfully addresses two challenges in neural stem cell transplantation therapy for intracerebral hemorrhage - low survival rate and uncontrolled differentiation - by designing a nanogel system, promoting white matter regeneration and improving behavioral performance in ICH mice.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Surgery
Haotian Jiang, Dewei Zhou, Pan Wang, Longwei Zeng, Jie Liu, Chao Tang, Gang Zhang, Xiaorong Tan, Nan Wu
Summary: The efficacy of fully endoscopic microvascular decompression in the treatment of glossopharyngeal neuralgia was evaluated in this study. A patient with glossopharyngeal neuralgia was successfully treated with fully endoscopic microvascular decompression, resulting in significant pain relief and no recurrence at the 6-month follow-up. The advantages of endoscopy in microvascular decompression were demonstrated, highlighting fully endoscopic microvascular decompression as an effective method for glossopharyngeal neuralgia.
FRONTIERS IN SURGERY
(2023)
Article
Clinical Neurology
Gang Zhang, Pan Wang, Junwei Wang, Dewei Zou, Hui Yao, Jie Liu, Chao Tang, Haotian Jiang, Xiaorong Tan, Nan Wu
Summary: The study explores the effectiveness and safety of a surgical strategy for removing PitNETs without incomplete pseudocapsules. The pseudocapsule-based extracapsular resection technique is applied in areas with pseudocapsule, while the intensive excision technique bounded by adjacent normal structures is adopted in other areas. The majority of suspicious tissues showed fragmental pseudocapsule and small tumor remnants.
FRONTIERS IN NEUROLOGY
(2023)
Review
Clinical Neurology
Kang Ma, Dawei Zhao, Xuegang Li, Haijun Duan, Chaojun Yan, Shi Wang, Lan Zeng, Kai Xu, Ying Lai, Beike Chen, Ning Mu, Chuanyan Yang, Yulian Quan, Zhengyang Li, Xiaoming Wang, Hua Feng, Fei Li
Summary: This article presents a case of multiple brain metastases caused by a cardiac myxoma, emphasizing the importance of prioritizing cardiac lesions and suggesting the efficacy of gamma knife radiosurgery combined with temozolomide as treatment. Treating the cardiac myxoma before addressing the metastasis sites is necessary.
FRONTIERS IN NEUROLOGY
(2023)
Article
Neurosciences
Chuan Shao, Junwei Wang, Pan Wang, Nan Wu
Summary: Retrospective analysis was conducted on patients with Cushing's disease (CD) who underwent endoscopic transsphenoidal surgery (ETS) with a layered peel strategy. The study found a low rate of surgical complications postoperatively and no recurrence events during follow-up. However, further studies are needed to confirm the effectiveness and safety of this approach due to the limited number of cases.
Review
Neurosciences
Gang Zhang, Junwei Wang, Pan Wang, Nan Wu
Summary: A case of middle-third falcine meningioma treated using the endoscopic ipsilateral interhemispheric approach is reported. Gross total tumor resection was achieved without complications, and the patient had a favorable recovery with no neurological deficit during the 24-month follow-up. The endoscopic ipsilateral interhemispheric approach is a feasible surgical strategy for falcine meningiomas and should be further explored.
Article
Cell Biology
Chao Xu, Pan Wang, Haiyan Guo, Chuan Shao, Bin Liao, Sheng Gong, Yanghao Zhou, Bingjie Yang, Haotian Jiang, Gang Zhang, Nan Wu
Summary: Glioma-associated macrophages (GAMs) are crucial in the tumor immune microenvironment (TIME) and their derived extracellular vesicles (M2-EVs) play a significant role in the malignancy and progression of GBM. M2-EVs enhance GBM cell invasion, migration, and epithelial-mesenchymal transition (EMT) signatures. MiR-146a-5p, a key factor in TIME regulation, is deficient in M2-EVs and its absence activates the TRAF6-IRAK1 complex, promoting EMT behaviors in GBM cells. Mouse model experiments showed that overexpression of miR-146a-5p or knockdown of TRAF6/IRAK1 extended survival, highlighting a potential therapeutic strategy targeting the GBM TIME.
CELL DEATH DISCOVERY
(2023)
Article
Biochemistry & Molecular Biology
Zhaoqi Zhang, Peiwen Guo, Liang Liang, Shiju Jila, Xufang Ru, Qiang Zhang, Jingyu Chen, Zhi Chen, Hua Feng, Yujie Chen
Summary: Chinese researchers at the Third Military Medical University in Chongqing have discovered that inhibiting the NLRP3 inflammasome protein can prevent the build-up of fluid on the brain after a hemorrhage. This protein complex triggers the formation of lipid droplets in the choroid plexus, damaging the blood-brain barrier and leading to hydrocephalus. Blocking NLRP3 activity improves the function of the barrier and could be a new therapeutic target for posthemorrhagic hydrocephalus.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
Article
Environmental Sciences
Yang Yang, Wang Ju, Quan Yu Lian, Yang Chuan Yan, Chen Xue Zhu, Lei Xue Jiao, Tan Liang, Feng Hua, Li Fei, Chen Tu Nan
Summary: This study aimed to investigate the underlying mechanism of memory deficit in vibroacoustic disease (VAD) caused by exposure to high intensity, low frequency noise (HI-LFN). The expression of TRPV4 gene was upregulated after HI-LFN exposure, and TRPV4 knockout mice showed better memory performance and more integrated nerve fibers after exposure. These findings suggest that TRPV4 up-regulation may contribute to memory impairment in VAD.
BIOMEDICAL AND ENVIRONMENTAL SCIENCES
(2023)