Overcoming an optimization plateau in the directed evolution of highly efficient nerve agent bioscavengers

Current and emerging strategies for organophosphate decontamination: special focus on hyperstable enzymes

(2016) Pauline Jacquet et al.   ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH

Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability

(2016) Adi Goldenzweig et al.   MOLECULAR CELL

Efficient laboratory evolution of computationally designed enzymes with low starting activities using fluorescence-activated droplet sorting

(2016) Richard Obexer et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Single treatment of VX poisoned guinea pigs with the phosphotriesterase mutant C23AL: Intraosseous versus intravenous injection

(2016) Timo Wille et al.   TOXICOLOGY LETTERS

Catalytic bioscavengers in nerve agent poisoning: A promising approach?

(2016) Franz Worek et al.   TOXICOLOGY LETTERS

The Moderately Efficient Enzyme: Futile Encounters and Enzyme Floppiness

(2015) Arren Bar-Even et al.   BIOCHEMISTRY

Variants of Phosphotriesterase for the Enhanced Detoxification of the Chemical Warfare Agent VR

(2015) Andrew N. Bigley et al.   BIOCHEMISTRY

Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently

(2015) Andrew Currin et al.   CHEMICAL SOCIETY REVIEWS

Protein stability: computation, sequence statistics, and new experimental methods

(2015) Thomas J Magliery   CURRENT OPINION IN STRUCTURAL BIOLOGY

Negative Epistasis and Evolvability in TEM-1 β-Lactamase—The Thin Line between an Enzyme's Conformational Freedom and Disorder

(2015) Eynat Dellus-Gur et al.   JOURNAL OF MOLECULAR BIOLOGY

FireProt: Energy- and Evolution-Based Computational Design of Thermostable Multiple-Point Mutants

(2015) David Bednar et al.   PLoS Computational Biology

Efficacy of the rePON1 mutant IIG1 to prevent cyclosarin toxicity in vivo and to detoxify structurally different nerve agents in vitro

(2014) Franz Worek et al.   ARCHIVES OF TOXICOLOGY

Post-exposure treatment of VX poisoned guinea pigs with the engineered phosphotriesterase mutant C23: A proof-of-concept study

(2014) Franz Worek et al.   TOXICOLOGY LETTERS

Engineering V-Type Nerve Agents Detoxifying Enzymes Using Computationally Focused Libraries

(2013) Izhack Cherny et al.   ACS Chemical Biology

Computational design gains momentum in enzyme catalysis engineering

(2013) Hein J. Wijma et al.   FEBS Journal

Enzymatic Neutralization of the Chemical Warfare Agent VX: Evolution of Phosphotriesterase for Phosphorothiolate Hydrolysis

(2013) Andrew N. Bigley et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Patterns of Epistasis between Beneficial Mutations in an Antibiotic Resistance Gene

(2013) Martijn F. Schenk et al.   MOLECULAR BIOLOGY AND EVOLUTION

Evolved Stereoselective Hydrolases for Broad-Spectrum G-Type Nerve Agent Detoxification

(2012) Moshe Goldsmith et al.   CHEMISTRY & BIOLOGY

Stabilizing Proteins from Sequence Statistics: The Interplay of Conservation and Correlation in Triosephosphate Isomerase Stability

(2012) Brandon J. Sullivan et al.   JOURNAL OF MOLECULAR BIOLOGY

Optimization of affinity, specificity and function of designed influenza inhibitors using deep sequencing

(2012) Timothy A Whitehead et al.   NATURE BIOTECHNOLOGY

Computational redesign of a mononuclear zinc metalloenzyme for organophosphate hydrolysis

(2012) Sagar D Khare et al.   Nature Chemical Biology

Evolutionary transitions to new DNA methyltransferases through target site expansion and shrinkage

(2012) Liat Rockah-Shmuel et al.   NUCLEIC ACIDS RESEARCH

Robust design and optimization of retroaldol enzymes

(2012) Eric A. Althoff et al.   PROTEIN SCIENCE

Diminishing returns and tradeoffs constrain the laboratory optimization of an enzyme

(2012) Nobuhiko Tokuriki et al.   Nature Communications

The Moderately Efficient Enzyme: Evolutionary and Physicochemical Trends Shaping Enzyme Parameters

(2011) Arren Bar-Even et al.   BIOCHEMISTRY

Directed evolution of hydrolases for prevention of G-type nerve agent intoxication

(2011) Rinkoo D Gupta et al.   Nature Chemical Biology

RosettaScripts: A Scripting Language Interface to the Rosetta Macromolecular Modeling Suite

(2011) Sarel J. Fleishman et al.   PLoS One

Diminishing Returns Epistasis Among Beneficial Mutations Decelerates Adaptation

(2011) H.-H. Chou et al.   SCIENCE

Epistasis between Beneficial Mutations and the Phenotype-to-Fitness Map for a ssDNA Virus

(2011) Darin R. Rokyta et al.   PLoS Genetics

Initial Mutations Direct Alternative Pathways of Protein Evolution

(2011) Merijn L. M. Salverda et al.   PLoS Genetics

Enzyme Promiscuity: A Mechanistic and Evolutionary Perspective

(2010) Olga Khersonsky and Dan S. Tawfik   Annual Review of Biochemistry

Diminishing Returns From Beneficial Mutations and Pervasive Epistasis Shape the Fitness Landscape for Rifampicin Resistance in Pseudomonas aeruginosa

(2010) R. C. MacLean et al.   GENETICS

Role of conformational sampling in computing mutation-induced changes in protein structure and stability

(2010) Elizabeth H. Kellogg et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Ultrahigh-Throughput FACS-Based Screening for Directed Enzyme Evolution

(2009) Guangyu Yang et al.   CHEMBIOCHEM

Stability effects of mutations and protein evolvability

(2009) Nobuhiko Tokuriki et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

RosettaLigand Docking with Full Ligand and Receptor Flexibility

(2008) Ian W. Davis et al.   JOURNAL OF MOLECULAR BIOLOGY