Review
Biochemistry & Molecular Biology
Mani S. Mahadevan, Ramesh S. Yadava, Mahua Mandal
Summary: DM1 is a multi-systemic disorder affecting various organs, with the heart being one of the most severely impacted. Cardiac conduction defects and arrhythmias are common in adults with DM1, leading to potential sudden death. Understanding of cardiac pathology in DM1 is still limited, with cardiac magnetic resonance imaging (CMR) emerging as a valuable biomarker.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Thiery De Serres-Berard, Siham Ait Benichou, Dominic Jauvin, Mohamed Boutjdir, Jack Puymirat, Mohamed Chahine
Summary: DM1 is a dominant genetic disease that can be treated by altering the chemical design of antisense oligonucleotides. Various strategies involving chemical modifications and conjugation with specific functional molecules have been shown to improve the potency in muscle and cardiac tissues. In addition, intrathecal administration can be used to treat central nervous system defects in DM1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Siham Ait Benichou, Dominic Jauvin, Thiery De Serres-Berard, Frank Bennett, Frank Rigo, Genevieve Gourdon, Mohamed Boutjdir, Mohamed Chahine, Jack Puymirat
Summary: This study evaluated the targeting effect of a new ligand-conjugated ASO in myotonic dystrophy type 1. The results showed that this ligand-conjugated ASO effectively degraded mutant hDMPK transcripts in a mouse model and improved muscle strength.
HUMAN GENE THERAPY
(2022)
Article
Multidisciplinary Sciences
Sneha Shah, Kevin J. Sharp, Sithara Raju Ponny, Jonathan Lee, Jonathan K. Watts, Elizabeth Berry- Kravis, Joel D. Richter
Summary: Aberrant alternative splicing of mRNAs leads to dysregulated gene expression in neurological disorders, such as fragile X syndrome (FXS). In this study, the researchers found hundreds of incorrectly expressed and spliced mRNAs in the blood and brain tissues of FXS individuals. They also discovered a previously unknown RNA isoform, FMR1-217, which is generated through mis-splicing of the FMR1 gene in FXS tissues. Treatment with antisense oligonucleotides (ASOs) could reduce FMR1-217 and restore normal gene expression and protein levels. These findings suggest that RNA-processing events in blood could serve as biomarkers for FXS and ASO treatment may be a potential therapy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Geriatrics & Gerontology
Giuseppe Biamonti, Angela Amato, Elisa Belloni, Anna Di Matteo, Lucia Infantino, Davide Pradella, Claudia Ghigna
Summary: Alzheimer's disease is the most common neurodegenerative disorder in the elderly, with approximately 20% of people over 80 affected. Recent studies have shown that Alternative Splicing is involved in gene expression reprogramming and splicing abnormalities in AD tissues. While the molecular causes of AD are still poorly understood, therapeutic approaches targeting aberrant AS regulation offer potential new treatments.
AGING CLINICAL AND EXPERIMENTAL RESEARCH
(2021)
Review
Clinical Neurology
Jean-Baptiste Brunet De Courssou, Alexandra Durr, David Adams, Jean-Christophe Corvol, Louise-Laure Mariani
Summary: Advances in targeted regulation of gene expression have opened up new therapeutic approaches for monogenic neurological diseases, with antisense therapies relying on modified nucleotide sequences showing promise in treating severe and previously untreatable disorders. Despite the success seen in various neurological disorders, there remain many unknowns and challenges to expanding these treatments to other diseases. Further research and development is needed to fully realize the potential of personalized medicine targeting the pathogenic roots of these conditions.
Article
Multidisciplinary Sciences
Josep Biayna, Helena Mazuelas, Bernat Gel, Ernest Terribas, Gabrijela Dumbovic, Inma Rosas, Juana Fernandez-Rodriguez, Ignacio Blanco, Elisabeth Castellanos, Meritxell Carrio, Conxi Lazaro, Eduard Serra
Summary: Neurofibromatosis Type 1 (NF1) is a genetic condition with cognitive impairment possibly related to alternative splicing of the NF1 gene. Modulation of the ratio between type I and type II isoforms can disrupt neuronal differentiation and deregulate signaling pathways. The study suggests a feedback loop involving neurofibromin-related signaling pathways.
SCIENTIFIC REPORTS
(2021)
Article
Medicine, Research & Experimental
Yuqin Tan, Tong Zheng, Zijun Su, Min Chen, Suxiang Chen, Rui Zhang, Ruojiao Wang, Ke Li, Ning Na
Summary: This study uncovers the regulatory axis of DNMT3B/NUDT21/APA/MORC2/DAPK1 in kidney renal clear cell carcinoma, revealing the role of APA in this cancer and the crosstalk between DNA methylation and APA.
Article
Biochemistry & Molecular Biology
Karima Relizani, Lucia Echevarria, Faouzi Zarrouki, Cecile Gastaldi, Chloe Dambrune, Philippine Aupy, Adrian Haeberli, Marek Komisarski, Thomas Tensorer, Thibaut Larcher, Fedor Svinartchouk, Cyrille Vaillend, Luis Garcia, Aurelie Goyenvalle
Summary: Tricyclo-DNA (tcDNA) is a promising oligonucleotide analog with therapeutic potential, especially when conjugated with palmitic acid for improved delivery to muscle tissues. This conjugation enhances the potency of tcDNA-ASO, resulting in functional improvement in dystrophic mice with significantly reduced dose, while also showing a promising safety profile for clinical development in neuromuscular diseases.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Mariapaola Izzo, Jonathan Battistini, Claudia Provenzano, Fabio Martelli, Beatrice Cardinali, Germana Falcone
Summary: Myotonic dystrophy type 1 (DM1) is a common muscular dystrophy that primarily affects skeletal and cardiac muscles, as well as the central nervous system. The disease is caused by the expansion of CTG repeats in the DMPK gene, leading to the production of a toxic CUG transcript. By directly targeting the CTG genomic tract, the expanded CUG transcript, or downstream signaling molecules, molecular therapeutic strategies show promise in treating DM1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Jessica Stoodley, Francisco Vallejo-Bedia, David Seone-Miraz, Manuel Debasa-Mouce, Matthew J. A. Wood, Miguel A. Varela
Summary: DM1 is a common muscular dystrophy that can potentially be treated with antisense therapy. Delivery of antisense molecules to target tissues remains a challenge, but conjugation to lipids, peptides, or antibodies has shown promise in improving therapeutic efficacy and tissue penetration. Clinical trials of antisense therapies are currently underway, raising hopes for an approved treatment for DM1 patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Genetics & Heredity
Muhammad Riaz Khan, Raymund J. Wellinger, Benoit Laurent
Summary: Long noncoding RNA (lncRNA) genes, like protein-coding genes, consist of introns and exons and undergo constitutive and/or alternative splicing after transcription. This review outlines the current understanding of lncRNA splice variants and their functional implications in cell biology.
TRENDS IN GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Junrui Zhou, Tingfang Li, Xin Geng, Lei Sui, Feng Wang
Summary: The study investigates the use of antisense oligonucleotides to inhibit telomerase activity as a potential new approach for cancer therapy. After chemical modification of some oligonucleotides, it was found that some of them could significantly reduce telomerase activity.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Pharmacology & Pharmacy
Sarah J. Overby, Estefania Cerro-Herreros, Jorge Espinosa-Espinosa, Irene Gonzalez-Martinez, Nerea Moreno, Juan M. Fernandez-Costa, Jordina Balaguer-Trias, Javier Ramon-Azcon, Manuel Perez-Alonso, Thorleif Moller, Beatriz Llamusi, Ruben Artero
Summary: The study demonstrates the therapeutic effects of using blockmiR antisense technology to increase MBNL protein in DM1 cells and mice. By blocking the binding sites of specific microRNAs, the mis-splicing, subcellular localization, and gene expression associated with Myotonic Dystrophy Type 1 can be rescued.
Article
Biochemistry & Molecular Biology
Natalia Bartys, Anna Pasternak, Jolanta Lisowiec-Wachnicka
Summary: This article presents detailed research on the optimization of bifunctional antisense oligonucleotides (BASOs) for modulating alternative splicing. The authors demonstrate the effectiveness of BASOs in regulating mutually exclusive alternative splicing of the PKM gene.
Article
Multidisciplinary Sciences
Praneet Chaturvedi, Yaseswini Neelamraju, Waqar Arif, Auinash Kalsotra, Sarath Chandra Janga
SCIENTIFIC REPORTS
(2015)
Article
Genetics & Heredity
Wencheng Li, Bei You, Mainul Hoque, Dinghai Zheng, Wenting Luo, Zhe Ji, Ji Yeon Park, Samuel I. Gunderson, Auinash Kalsotra, James L. Manley, Bin Tian
Article
Biology
Sandip Chorghade, Joseph Seimetz, Russell Emmons, Jing Yang, Stefan M. Bresson, Michael De Lisio, Gianni Parise, Nicholas K. Conrad, Auinash Kalsotra
Article
Biochemistry & Molecular Biology
Ravi K. Singh, Zheng Xia, Christopher S. Bland, Auinash Kalsotra, Marissa A. Scavuzzo, Tomaz Curk, Jernej Ule, Wei Li, Thomas A. Cooper
Article
Biochemistry & Molecular Biology
Sushant Bangru, Waqar Arif, Joseph Seimetz, Amruta Bhate, Jackie Chen, Edrees H. Rashan, Russ P. Carstens, Sayeepriyadarshini Anakk, Auinash Kalsotra
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2018)
Article
Multidisciplinary Sciences
Jimena Giudice, Zheng Xia, Eric T. Wang, Marissa A. Scavuzzo, Amanda J. Ward, Auinash Kalsotra, Wei Wang, Xander H. T. Wehrens, Christopher B. Burge, Wei Li, Thomas A. Cooper
NATURE COMMUNICATIONS
(2014)
Article
Medicine, Research & Experimental
Jeongeun Hyun, Zhaoli Sun, Ali Reza Ahmadi, Sushant Bangru, Ullas Chembazhi, Kuo Du, Tianyi Chen, Hidekazu Tsukamoto, Ivan Rusyn, Auinash Kalsotra, Anna Mae Diehl
JOURNAL OF CLINICAL INVESTIGATION
(2020)
Article
Biochemistry & Molecular Biology
Ullas Chembazhi, Sushant Bangru, Mikel Hernaez, Auinash Kalsotra
Summary: The liver has a unique ability to regenerate in response to injuries, with a subset of hepatocytes reactivating early-postnatal-like gene expression program to proliferate and another population of metabolically active cells compensating for temporary deficits in liver function. The study also reveals the spatial and metabolic dynamics of hepatocyte proliferation after partial hepatectomy, with midlobular cells proliferating nearby while portal and central vein proximal hepatocytes remain metabolically active to preserve liver functions. Intercellular crosstalk and cellular reprogramming play a crucial role in balancing metabolic and proliferative requirements during liver regeneration.
Article
Multidisciplinary Sciences
Bhoomika Mathur, Asif Shajahan, Waqar Arif, Qiushi Chen, Nicholas J. Hand, Lara K. Abramowitz, Kristina Schoonjans, Daniel J. Rader, Auinash Kalsotra, John A. Hanover, Parastoo Azadi, Sayeepriyadarshini Anakk
Summary: The nuclear receptors FXR and SHP play a role in maintaining glycoprotein diversity in the liver by regulating the N-glycosylation pathway. Activation of FXR suppressed Mgat5, while deletion of Shp induced St3gal6 and St6gal1, leading to an increase in glycan complexity and altered Golgi apparatus structure and secretion patterns.
Article
Biology
Jianhao Peng, Guillermo Serrano, Ian M. Traniello, Maria E. Calleja-Cervantes, Ullas V. Chembazhi, Sushant Bangru, Teresa Ezponda, Juan Roberto Rodriguez-Madoz, Auinash Kalsotra, Felipe Prosper, Idoia Ochoa, Mikel Hernaez
Summary: Single-cell RNA-Sequencing is a powerful tool for studying complex regulatory interactions between diverse cell phenotypes. However, current methods are limited in their ability to uncover complex regulatory relationships across related cell types. The presented framework, SimiC, overcomes this limitation by inferring distinct regulatory dynamics per phenotype. SimiC has shown to uncover key regulatory patterns missed by previous methods, with implications for systems biology.
COMMUNICATIONS BIOLOGY
(2022)
Article
Developmental Biology
Surabhi Sonam, Sushant Bangru, Kimberly J. Perry, Ullas Chembazhi, Auinash Kalsotra, Jonathan J. Henry
Summary: This study performed single-cell RNA sequencing of corneal cells and revealed an increase in cellular diversity and transcriptional profile during cornea development and maturation. The study also identified specific basal cell clusters in larval and adult cornea that expressed stem cell markers, potentially being larval and adult CESCs.
DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Jessica M. Derham, Auinash Kalsotra
Summary: Alternative splicing is a mechanism that is widely used in gene expression and functionality. It relies on RNA binding proteins to bind target sequences in pre-mRNAs, allowing for the inclusion or skipping of different exons. The epithelial splicing regulatory proteins (ESRPs) are a recently discovered family of RNA binding proteins. This article discusses their structure, physiological function, and their roles in coordinating splicing and functional output in various contexts.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2023)
Article
Gastroenterology & Hepatology
Bhoomika Mathur, Waqar Arif, Megan E. Patton, Rahiman Faiyaz, Jian Liu, Jennifer Yeh, Sanjiv Harpavat, Kristina Schoonjans, Auinash Kalsotra, Antony M. Wheatley, Sayeepriyadarshini Anakk
Review
Biochemistry & Molecular Biology
Waciar Arif, Gandhar Datar, Auinash Kalsotra
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2017)