Article
Chemistry, Multidisciplinary
Emmanouil Archontakis, Laura Woythe, Bas van Hoof, Lorenzo Albertazzi
Summary: Antibody-functionalized nanoparticles have advantages in drug delivery and biosensing, but controlling the orientation of antibodies on the nanoparticle surface is a challenge. This study introduces a super-resolution optical technique to quantitatively evaluate the orientation of antibodies on individual nanoparticles and identifies particles with different functional to total ratios.
NANOSCALE ADVANCES
(2022)
Article
Biochemistry & Molecular Biology
Sebastien Rinaldetti, Qiong Zhou, Joshua M. Abbott, Florus C. de Jong, Hector Esquer, James C. Costello, Dan Theodorescu, Daniel LaBarbera
Summary: This study discovered novel subtype-stratified therapy approaches for bladder cancer based on high-content screening, and correlated drug sensitivity with functional genomics to validate and understand the underlying mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Maria Galvez-Llompart, Riccardo Zanni, Ramon Garcia-Domenech, Jorge Galvez
Summary: Despite being considered an orphan disease, amyotrophic lateral sclerosis (ALS) is seeing a rapid increase in prevalence, making it difficult to find effective treatments. Computational methods based on receptors or ligands have the potential to improve the success rate of potential drug candidates and reduce resources and time. This study presents a computational strategy based on Molecular Topology to create a reliable tool for identifying and repurposing potential drugs for ALS treatment in the early stages.
Article
Multidisciplinary Sciences
Takeru Takagi, Tasuku Ueno, Keisuke Ikawa, Daisuke Asanuma, Yusuke Nomura, Shin-nosuke Uno, Toru Komatsu, Mako Kamiya, Kenjiro Hanaoka, Chika Okimura, Yoshiaki Iwadate, Kenzo Hirose, Tetsuo Nagano, Kaoru Sugimura, Yasuteru Urano
Summary: The discovery of a new actin-binding small molecule presents an important advance in the field, offering the opportunity to design and synthesize new functional molecules. This molecule enables visualization of the actin cytoskeleton and allows for highly specific control of actin filaments in living cells.
Review
Medicine, General & Internal
Kong-Fu Zhu, Chuang Yuan, Yong-Ming Du, Kai-Lei Sun, Xiao-Kang Zhang, Horst Vogel, Xu-Dong Jia, Yuan-Zhu Gao, Qin-Fen Zhang, Da-Ping Wang, Hua-Wei Zhang
Summary: Drug discovery is a crucial, albeit time- and effort-consuming, part of healthcare with tremendous benefits to human lifespan and life quality. Cryo-electron microscopy (cryo-EM) has emerged as a powerful tool in accelerating drug development, despite its limitations in resolution, speed, and throughput. Cryo-EM techniques are increasingly applied in various aspects of drug discovery, including structure-based drug design, fragment-based drug discovery, and antibody drug development. The combination of cryo-EM and artificial intelligence (AI) shows promise in overcoming the limitations of cryo-EM and shaping the future development of this technique.
MILITARY MEDICAL RESEARCH
(2023)
Review
Medicine, Research & Experimental
Long Nguyen, Carlos Caldas
Summary: Advances in sequencing technology have allowed detailed characterization of human malignancies, but translating this information into clinically relevant outcomes has been slow. Models to study human cancers have been established, leading to the identification of genetic dependencies that can be targeted with drug therapy. However, the discovery of biomarkers for predicting treatment response remains a challenge in many cancers.
EMBO MOLECULAR MEDICINE
(2021)
Review
Pharmacology & Pharmacy
Bolormaa Baljinnyam, Michael Ronzetti, Anton Simeonov
Summary: Luminescence-based technologies, including chemiluminescence and bioluminescence, have extensive applications in drug discovery due to their high signal-to-noise ratio and lack of excitation light source requirement. This review provides an overview of luciferase-based technologies and discusses their recent advances and use cases in drug discovery. Improved luminescent reporter systems will lead to broader applications of luminescence-based technologies in early-stage drug discovery.
EXPERT OPINION ON DRUG DISCOVERY
(2023)
Article
Immunology
Joerg P. J. Mueller, Michael Dobosz, Nils O'Brien, Nassri Abdoush, Anna Maria Giusti, Martin Lechmann, Franz Osl, Ann-Katrin Wolf, Estibaliz Arellano-Viera, Haroon Shaikh, Markus Sauer, Andreas Rosenwald, Frank Herting, Pablo Umana, Sara Colombetti, Thomas Poeschinger, Andreas Beilhack
Summary: Advancement of immunotherapy strategies requires refined tools for preclinical research. Light sheet fluorescence microscopy (LSFM) is a powerful imaging technique, but current tissue processing procedures limit its broad applications in immunological research. In this study, we developed a simple and harmonized protocol for processing, clearing, and imaging mouse organs and mouse bodies to enable comprehensive studies of drug targets. Using this protocol in combination with LSFM, we discovered new sites of high expression of EpCAM, a target for antibody-based immunotherapies, in mouse organs. These findings were confirmed in human samples and may have important implications for clinical translation of EpCAM-targeting immunotherapies. We propose that this new protocol, called ROCKETS, can set new standards for preclinical evaluation of immunotherapeutic strategies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Physical
Mingyu Jeon, Ohmin Kwon, Jihan Kim
Summary: In this study, a screening algorithm was developed to construct 2D-MOF@3D-MOF composite structures, prioritizing synthesizability. Several pairs of candidate materials were identified, with Ni-HHTP@UiO-66 being one of the potential candidates. The algorithm-passed 2D-MOF@3D-MOF composite exhibited superior mechanical strength compared to the mismatched composite.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Review
Pharmacology & Pharmacy
Anil K. Giri, Aleksander Ianevski
Summary: The interactions between leukemic blasts and cells within the bone marrow environment play a crucial role in oncogenesis, cancer stem cell survival, and drug resistance in hematological cancers. Recent advancements in drug screening technologies and computational algorithms are helping to target previously undruggable proteins and complex pathways, particularly in the context of leukemic cell-bone microenvironment interactions.
EXPERT OPINION ON DRUG DISCOVERY
(2022)
Review
Pharmacology & Pharmacy
Vojtech Dvorak, Giulio Superti-Furga
Summary: Solute carriers (SLCs) are the largest group of membrane transporters in the human genome, and they are central in controlling metabolism compartmentalization, with most of this superfamily being linked to human diseases. Despite being considered attractive therapeutic targets, many SLCs still lack functional and structural annotations.
EXPERT OPINION ON DRUG DISCOVERY
(2023)
Proceedings Paper
Computer Science, Artificial Intelligence
Luis Carlos Rivera Monroy, Leonhard Rist, Martin Eberhardt, Christian Ostalecki, Andreas Baur, Julio Vera, Katharina Breininger, Andreas Maier
Summary: Histopathology imaging is crucial for the diagnosis and treatment of skin diseases. This work proposes a pipeline that characterizes suspected melanoma samples using Multi-Epitope-Ligand Cartography (MELC) and trains a graph neural network to achieve high accuracy in skin disease classification.
2023 IEEE 20TH INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING, ISBI
(2023)
Article
Chemistry, Medicinal
Mingming Zhang, Wei Wei, Chengjun Peng, Xiaodong Ma, Xiao He, Heng Zhang, Mingkang Zhou
Summary: The study unveils a novel series of mTOR/HDAC bi-functional inhibitors, with compound 50 showing high dual-target inhibitory and anti-proliferative activities, making it a potential anti-cancer agent for further investigation.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Biochemical Research Methods
Jesse T. Chao, Calvin D. Roskelley, Christopher J. R. Loewen
Summary: This paper introduces a new method to rapidly assess variant function using cloud deep learning. A software toolkit MAPS is developed for extracting and classifying cell-level features using deep learning, which helps users leverage cloud-based services for their specific experimental conditions. By integrating high-content microscopy and MAPS, an effective variant functionalization workflow is demonstrated for assessing missense variants of the PTEN tumor suppressor.
BMC BIOINFORMATICS
(2021)
Article
Food Science & Technology
Gurjeet Kaur, Gagandeep Kaur Sidhu, Preetinder Kaur, Amarjit Kaur
Summary: The present study found that the combined ozonation-roasting treatment had a significant influence on the moisture content, color, functional, structural, and textural components, as well as aflatoxin content in groundnut kernels. This treatment led to a reduction in moisture content, an increase in total phenolic content and antioxidant capacity, and a more uniform particle orientation. Additionally, the treatment resulted in a considerable reduction in aflatoxin content.
JOURNAL OF TEXTURE STUDIES
(2022)
Article
Multidisciplinary Sciences
Reyk Hillert, Anne Gieseler, Andreas Krusche, Daniel Humme, Hans-Joachim Roewert-Huber, Wolfram Sterry, Peter Walden, Walter Schubert
SCIENTIFIC REPORTS
(2016)
Review
Biochemical Research Methods
Walter Schubert, Marcus Bode, Reyk Hillert, Andreas Krusche, Manuela Friedenberger
EXPERT REVIEW OF PROTEOMICS
(2008)
Article
Computer Science, Software Engineering
Steffen Oeltze, Wolfgang Freiler, Reyk Hillert, Helmut Doleisch, Bernhard Preim, Walter Schubert
IEEE TRANSACTIONS ON VISUALIZATION AND COMPUTER GRAPHICS
(2011)
Article
Biotechnology & Applied Microbiology
Julia Herold, Walter Schubert, Tim W. Nattkemper
JOURNAL OF BIOTECHNOLOGY
(2010)
Article
Biotechnology & Applied Microbiology
Andrei Barysenka, Andreas W. M. Dress, Walter Schubert
JOURNAL OF BIOTECHNOLOGY
(2010)
Review
Biotechnology & Applied Microbiology
Walter Schubert
JOURNAL OF BIOTECHNOLOGY
(2010)
Review
Biochemistry & Molecular Biology
Walter Schubert
JOURNAL OF MOLECULAR RECOGNITION
(2014)
Article
Biochemical Research Methods
Walter Schubert, Anne Gleseler, Andreas Krusche, Reyk Hillert
JOURNAL OF PROTEOME RESEARCH
(2009)
Article
Biochemical Research Methods
Sayantan Bhattacharya, George Mathew, Ernie Ruban, David B. A. Epstein, Andreas Krusche, Reyk Hillert, Walter Schubert, Michael Khan
JOURNAL OF PROTEOME RESEARCH
(2010)
Article
Biochemical Research Methods
Walter Schubert, Anne Gieseler, Andreas Krusche, Peter Serocka, Reyk Hillert
Letter
Multidisciplinary Sciences
Walter Schubert, Andreas Dress, Mika Ruonala, Andreas Krusche, Reyk Hillert, Anne Gieseler, Peter Walden
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2014)
Article
Biochemical Research Methods
Marcus Bode, Martin Irmler, Manuela Friedenberger, Caroline May, Klaus Jung, Christian Stephan, Helmut E. Meyer, Christiane Lach, Reyk Hillert, Andreas Krusche, Johannes Beckers, Katrin Marcus, Walter Schubert
Article
Mathematics, Applied
Andrei Barysenka, Andreas W. M. Dress, Walter Schubert
EAST ASIAN JOURNAL ON APPLIED MATHEMATICS
(2011)
Editorial Material
Biochemical Research Methods
Walter Schubert