Article
Multidisciplinary Sciences
Alessia Capone, Chiara Naro, Manuela Bianco, Marco De Bardi, Floriane Noel, Paolo Macchi, Luca Battistini, Vassili Soumelis, Elisabetta Volpe, Claudio Sette
Summary: By analyzing the transcriptome of human Th17 cells, researchers identified three time-regulated modules corresponding to early, late, and persistent stages of differentiation, with inflammatory genes predominantly found in the persistent module and regulatory genes induced in late or persistent stages. Key inflammatory genes identified include IL17A, IL17F, IL26, IL6, IFNG, IFNK, LTA, IL1A, PDGFA, HOPX, and SOX2. This study provides insights into the stepwise differentiation program of human Th17 cells and offers potential therapeutic targets for Th17-related autoimmune diseases.
Article
Chemistry, Physical
Wei Yin, Gaoxia Liu, Jinhong Li, Zhuan Bian
Summary: The study revealed various cell populations in human dental pulp, including dental pulp stem cells, T cells, macrophages, etc., which support each other through growth signals. Pulp cells have the greatest communication with other cell types, while T cells have the least. T cells and endothelial cells play a role in monitoring bacterial invasion through specific receptor expressions. These findings provide insights into the cellular composition of normal dental pulp.
Article
Developmental Biology
Jessica M. Toothaker, Oluwabunmi Olaloye, Blake T. McCourt, Collin C. McCourt, Tatiana N. Silva, Rebecca M. Case, Peng Liu, Dean Yimlamai, George Tseng, Liza Konnikova
Summary: Maintenance of a healthy pregnancy depends on the balance between the fetal and maternal immune systems. This study used advanced techniques to investigate the immune system in mid-gestation human placental villi and found multiple subtypes of fetal immune cells, including memory T cells. It was also discovered that T cells isolated from placental villi had enhanced proliferative capacity compared to adult T cells.
Article
Biochemistry & Molecular Biology
Sara Scaramuzzino, Delphine Potier, Robin Ordioni, Pierre Grenot, Dominique Payet-Bornet, Herve Luche, Bernard Malissen
Summary: The study demonstrated that γδ T-cell lineage development not only relies on the signaling of γδ TCR but also requires the regulatory role of LAT in mice lacking LAT expression.
Article
Immunology
Michael Delacher, Malte Simon, Lieke Sanderink, Agnes Hotz-Wagenblatt, Marina Wuttke, Kathrin Schambeck, Lisa Schmidleithner, Sebastian Bittner, Asmita Pant, Uwe Ritter, Thomas Hehlgans, Dania Riegel, Verena Schneider, Florian Kai Groeber-Becker, Andreas Eigenberger, Claudia Gebhard, Nicholas Strieder, Alexander Fischer, Michael Rehli, Petra Hoffmann, Matthias Edinger, Till Strowig, Jochen Huehn, Christian Schmidl, Jens M. Werner, Lukas Prantl, Benedikt Brors, Charles D. Imbusch, Markus Feuerer
Summary: Research identified features of human tissue Treg cells with tissue repair functions, which show similarities in gene expression profiles at the single-cell level with murine cells, and shared characteristics with T follicular helper-like cells. Inducing differentiation of human Treg cells into Tfh-like cells can partially replicate the regenerative characteristics of tissue Treg cells.
Review
Immunology
Yunmin Jung
Summary: This review summarizes the current understanding of the pre-organization of key surface receptors on the plasma membrane of resting T cells and discusses their potential role in early T cell activation events.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yanqiang Ding, Liuyang Zhao, Guoping Wang, Yu Shi, Gang Guo, Changan Liu, Zigui Chen, Olabisi Oluwabukola Coker, Junjun She, Jun Yu
Summary: This study used PacBio sequencing to reveal the DNA methylation landscape of gut phages. The research found that methylation plays an important role in the taxonomy and interaction with hosts of phages, and also discovered the presence of restriction-modification systems in some phage genomes, which aid in evading clearance by the host.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Immunology
Kevin Mulder, Amit Ashok Patel, Wan Ting Kong, Cecile Piot, Evelyn Halitzki, Garett Dunsmore, Shabnam Khalilnezhad, Sergio Erdal Irac, Agathe Dubuisson, Marion Chevrier, Xiao Meng Zhang, John Kit Chung Tam, Tony Kiat Hon Lim, Regina Men Men Wong, Rhea Pai, Ahmed Ibrahim Samir Khalil, Pierce Kah Hoe Chow, Suny Z. Wu, Ghamdan Al-Eryani, Daniel Roden, Alexander Swarbrick, Jerry Kok Yen Chan, Salvatore Albani, Lisa Derosa, Laurence Zitvogel, Ankur Sharma, Jinmiao Chen, Aymeric Silvin, Antonio Bertoletti, Camille Bleriot, Charles-Antoine Dutertre, Florent Ginhoux
Summary: This study integrated a large number of mononuclear phagocytes from 13 tissues to generate a MNP single-cell RNA compendium, revealing conserved gene signatures and specialized cell subsets across multiple tissues. Specific macrophage populations were found to be expanded in cancer and inflammation, with tumor-associated macrophage populations present in all neoplastic tissues.
Article
Biochemical Research Methods
Xiaoou Ren, Anthony E. Getschman, Samuel Hwang, Brian F. Volkman, Thomas Klonisch, David Levin, Min Zhao, Susy Santos, Song Liu, Jasmine Cheng, Francis Lin
Summary: A three-dimensional skin-on-chip model was developed to study the migration of human T lymphocytes in inflammatory microenvironments. Results showed that T cell migration can be inhibited by engineered CCL20 locked dimer and S1P background, while inflammatory cytokine stimulated epithelial cells can induce T cell transmigration in the model.
Article
Multidisciplinary Sciences
David M. Schauder, Jian Shen, Yao Chen, Moujtaba Y. Kasmani, Matthew R. Kudek, Robert Burns, Weiguo Cui
Summary: During acute viral infections, CD8 T cells encounter various antigenic and inflammatory signals, leading to differentiation into memory cells or death. Research has shown that memory precursor effector cells maintain open enhancers for key memory genes, with a high enrichment of E2A binding sites. E2A directly regulates the accessibility of enhancers for memory-related genes, increasing the frequency of memory precursor effector cells and accelerating memory cell formation while reducing short-lived effector cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Medicine, Research & Experimental
Wei Zhang, Qian Zhang, Chao Zhu, Zhiyuan Shi, Chen Shao, Yujie Chen, Nan Wang, Yanxia Jiang, Qing Liang, Kejia Wang
Summary: This study revealed diminished T-cell infiltration and increased T-cell receptor immune repertoire diversity in clear cell renal cell cancer (ccRCC). Furthermore, Vimentin expression in ccRCC tissue was positively correlated with the presence of positive T-cell markers. The immune repertoire sequencing results showed increased diversity in the T-cell receptor repertoire in ccRCC.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Edyta Janik-Karpinska, Michal Ceremuga, Marcin Niemcewicz, Ewelina Synowiec, Tomasz Sliwinski, Maksymilian Stela, Michal Bijak
Summary: This study demonstrates that T-2 toxin has genotoxic effects on human skin fibroblast cells, potentially causing damage to nuclear DNA and leading to changes in gene expression related to inflammation and repair processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Edyta Janik-Karpinska, Michal Ceremuga, Magdalena Wieckowska, Monika Szyposzynska, Marcin Niemcewicz, Ewelina Synowiec, Tomasz Sliwinski, Michal Bijak
Summary: T-2 toxin, produced by various Fusarium species, can infect crops such as wheat, barley, and corn. This in vitro study reveals that T-2 toxin induces necrosis in human skin fibroblast cells, providing new insights into its toxic mechanism on the skin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Vivian Hathuc, Friederike Kreisel
Summary: This review discusses the genetic landscape of peripheral T-cell lymphoma (PTCL) and its role in subclassification, prognosis, and potential targeted therapy. The article emphasizes the use of a multiparameter approach in the diagnosis and classification, and introduces the latest applications of gene expression profiling and next-generation sequencing (NGS) techniques in the pathobiology of PTCL.
Article
Genetics & Heredity
Xin Wang, Zhenyi Xu, Zhilin Liu, Weihao Lin, Zheng Cao, Xiaoli Feng, Yibo Gao, Jie He
Summary: This study aimed to investigate the immune cell infiltration in lung adenocarcinoma (LUAD) and develop a predictive system for clinical outcomes. Through transcriptomic analysis and classification of LUAD samples, it was found that TMEscore is a reliable and independent prognostic biomarker that can distinguish the survival rate and immune phenotype of LUAD patients.
FRONTIERS IN GENETICS
(2022)
Review
Biochemical Research Methods
Walter Schubert
Review
Biochemical Research Methods
Walter Schubert, Marcus Bode, Reyk Hillert, Andreas Krusche, Manuela Friedenberger
EXPERT REVIEW OF PROTEOMICS
(2008)
Article
Computer Science, Software Engineering
Steffen Oeltze, Wolfgang Freiler, Reyk Hillert, Helmut Doleisch, Bernhard Preim, Walter Schubert
IEEE TRANSACTIONS ON VISUALIZATION AND COMPUTER GRAPHICS
(2011)
Article
Biotechnology & Applied Microbiology
Julia Herold, Walter Schubert, Tim W. Nattkemper
JOURNAL OF BIOTECHNOLOGY
(2010)
Article
Biotechnology & Applied Microbiology
Andrei Barysenka, Andreas W. M. Dress, Walter Schubert
JOURNAL OF BIOTECHNOLOGY
(2010)
Review
Biotechnology & Applied Microbiology
Walter Schubert
JOURNAL OF BIOTECHNOLOGY
(2010)
Review
Biochemistry & Molecular Biology
Walter Schubert
JOURNAL OF MOLECULAR RECOGNITION
(2014)
Article
Biochemical Research Methods
Walter Schubert, Anne Gleseler, Andreas Krusche, Reyk Hillert
JOURNAL OF PROTEOME RESEARCH
(2009)
Article
Biochemical Research Methods
Sayantan Bhattacharya, George Mathew, Ernie Ruban, David B. A. Epstein, Andreas Krusche, Reyk Hillert, Walter Schubert, Michael Khan
JOURNAL OF PROTEOME RESEARCH
(2010)
Article
Biochemical Research Methods
Walter Schubert, Anne Gieseler, Andreas Krusche, Peter Serocka, Reyk Hillert
Letter
Multidisciplinary Sciences
Walter Schubert, Andreas Dress, Mika Ruonala, Andreas Krusche, Reyk Hillert, Anne Gieseler, Peter Walden
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2014)
Article
Biochemical Research Methods
Marcus Bode, Martin Irmler, Manuela Friedenberger, Caroline May, Klaus Jung, Christian Stephan, Helmut E. Meyer, Christiane Lach, Reyk Hillert, Andreas Krusche, Johannes Beckers, Katrin Marcus, Walter Schubert
Article
Mathematics, Applied
Andrei Barysenka, Andreas W. M. Dress, Walter Schubert
EAST ASIAN JOURNAL ON APPLIED MATHEMATICS
(2011)
Editorial Material
Biochemical Research Methods
Walter Schubert